The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Mycobacterium tuberculosis is extraordinarily sensitive to killing by a vitamin C-induced Fenton reaction
Open accessAbstract
Drugs that kill tuberculosis more quickly could shorten chemotherapy significantly. In Escherichia coli, a common mechanism of cell death by bactericidal antibiotics involves the generation of highly reactive hydroxyl radicals via the Fenton reaction. Here we show that vitamin C, a compound known to drive the Fenton reaction, sterilizes cultures of drug-susceptible and drug-resistant Mycobacterium tuberculosis, the causative agent of tuberculosis. While M. tuberculosis is highly susceptible to killing by vitamin C, other Gram-positive and Gram-negative pathogens are not. The bactericidal activity of vitamin C against M. tuberculosis is dependent on high ferrous ion levels and reactive oxygen species production, and causes a pleiotropic effect affecting several biological processes. This study enlightens the possible benefits of adding vitamin C to an anti-tuberculosis regimen and suggests that the development of drugs that generate high oxidative burst could be of great use in tuberculosis treatment. New approaches to kill Mycobacterium tuberculosis are required to improve TB therapy. Vilchèze et al. now demonstrate that vitamin C-derived reactive oxygen species lead to sterilization of drug-susceptible and drug-resistant M. tuberculosisoffering new possibilities for drug development.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
LXXIII.—Oxidation of tartaric acid in presence of iron
Fenton, H. J. H. (1894) · J. Chem. Soc., Trans. · doi:10.1039/ct8946500899 · Cited by 3,179
A Common Mechanism of Cellular Death Induced by Bactericidal Antibiotics
Kohanski, M. A., Dwyer, D. J., Hayete, B., Lawrence, C. A., & Collins, J. J. (2007) · Cell · doi:10.1016/j.cell.2007.06.049 · Cited by 2,982
The catalytic decomposition of hydrogen peroxide by iron salts
Haber, F., & Weiss, J. (1934) · Proceedings of the Royal Society of London. Series A - Mathematical and Physical Sciences · doi:10.1098/rspa.1934.0221 · Cited by 2,571
Vitamin C as an Antioxidant: Evaluation of Its Role in Disease Prevention
Padayatty, S. J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J.-H., Chen, S., Corpe, C., Dutta, A., Dutta, S. K., et al. (2003) · Journal of the American College of Nutrition · doi:10.1080/07315724.2003.10719272 · Cited by 1,949
Ascorbate is an outstanding antioxidant in human blood plasma
Frei, B., England, L., & Ames, B. N. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.16.6377 · Cited by 1,870
Vitamin C in Human Health and Disease Is Still a Mystery? An Overview
Naidu, K. A. (2003) · Nutrition Journal · doi:10.1186/1475-2891-2-7 · Cited by 810
Vitamin C exhibits pro-oxidant properties
Podmore, I. D., Griffiths, H. R., Herbert, K. E., Mistry, N., Mistry, P., & Lunec, J. (1998) · Nature · doi:10.1038/33308 · Cited by 800
Cell Death from Antibiotics Without the Involvement of Reactive Oxygen Species
Liu, Y., & Imlay, J. A. (2013) · Science · doi:10.1126/science.1232751 · Cited by 593
Kohanski, M. A., Dwyer, D. J., Wierzbowski, J., Cottarel, G., & Collins, J. J. (2008) · Cell · doi:10.1016/j.cell.2008.09.038 · Cited by 574
Colorimetric ferrozine-based assay for the quantitation of iron in cultured cells
Riemer, J., Hoepken, H. H., Czerwinska, H., Robinson, S. R., & Dringen, R. (2004) · Analytical Biochemistry · doi:10.1016/j.ab.2004.03.049 · Cited by 563
Killing by Bactericidal Antibiotics Does Not Depend on Reactive Oxygen Species
Keren, I., Wu, Y., Inocencio, J., Mulcahy, L. R., & Lewis, K. (2013) · Science · doi:10.1126/science.1232688 · Cited by 550
Oxidation of the Guanine Nucleotide Pool Underlies Cell Death by Bactericidal Antibiotics
Foti, J. J., Devadoss, B., Winkler, J. A., Collins, J. J., & Walker, G. C. (2012) · Science · doi:10.1126/science.1219192 · Cited by 497
Rodriguez, G. M., Voskuil, M. I., Gold, B., Schoolnik, G. K., & Smith, I. (2002) · Infection and Immunity · doi:10.1128/iai.70.7.3371-3381.2002 · Cited by 490
Evolution of Drug Resistance in Mycobacterium tuberculosis : Clinical and Molecular Perspective
Gillespie, S. H. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.2.267-274.2002 · Cited by 412
Dwyer, D. J., Camacho, D. M., Kohanski, M. A., Callura, J. M., & Collins, J. J. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.04.027 · Cited by 404
Biosynthesis and Functions of Mycothiol, the Unique Protective Thiol of Actinobacteria
Newton, G. L., Buchmeier, N., & Fahey, R. C. (2008) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00008-08 · Cited by 353
Vilchèze, C., Morbidoni, H. R., Weisbrod, T. R., Iwamoto, H., Kuo, M., Sacchettini, J. C., & Jacobs, W. R. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.14.4059-4067.2000 · Cited by 283
Self-citationHODGES, R. E., BAKER, E. M., HOOD, J., SAUBERLICH, H. E., & MARCH, S. C. (1969) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/22.5.535 · Cited by 239
A Comparative Lipidomics Platform for Chemotaxonomic Analysis of Mycobacterium tuberculosis
Layre, E., Sweet, L., Hong, S., Madigan, C. A., Desjardins, D., Young, D. C., Cheng, T.-Y., Annand, J. W., Kim, K., Shamputa, I. C., et al. (2011) · Chemistry & Biology · doi:10.1016/j.chembiol.2011.10.013 · Cited by 205
Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis
Vilchèze, C., Av‐Gay, Y., Attarian, R., Liu, Z., Hazbón, M. H., Colangeli, R., Chen, B., Liu, W., Alland, D., Sacchettini, J. C., et al. (2008) · Molecular Microbiology · doi:10.1111/j.1365-2958.2008.06365.x · Cited by 168
Self-citationIoerger, T. R., Koo, S., No, E.-G., Chen, X., Larsen, M. H., Jacobs, W. R., Pillay, M., Sturm, A. W., & Sacchettini, J. C. (2009) · PLoS ONE · doi:10.1371/journal.pone.0007778 · Cited by 165
Self-citationGilleron, M., Ronet, C., Mempel, M., Monsarrat, B., Gachelin, G., & Puzo, G. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m103908200 · Cited by 161
Biosynthesis of Mycobacterial Lipoarabinomannan
Besra, G. S., Morehouse, C. B., Rittner, C. M., Waechter, C. J., & Brennan, P. J. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.29.18460 · Cited by 161
Model Studies of the Iron-Catalysed Haber-Weiss Cycle and the Ascorbate-Driven Fenton Reaction
Burkitt, M. J., & Gilbert, B. C. (1990) · Free Radical Research Communications · doi:10.3109/10715769009149895 · Cited by 161
Sartain, M. J., Dick, D. L., Rithner, C. D., Crick, D. C., & Belisle, J. T. (2011) · Journal of Lipid Research · doi:10.1194/jlr.m010363 · Cited by 156
Taneja, N. K., Dhingra, S., Mittal, A., Naresh, M., & Tyagi, J. S. (2010) · PLoS ONE · doi:10.1371/journal.pone.0010860 · Cited by 130
Newton, G. L., Unson, M. D., Anderberg, S. J., Aguilera, J. A., Oh, N. N., delCardayré, S. B., Av‐Gay, Y., & Fahey, R. C. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0156 · Cited by 97
Preparation of Cell-Wall Fractions from Mycobacteria
Besra, G. S. (2003) · Humana Press eBooks · doi:10.1385/0-89603-471-2:91 · Cited by 94
Spontaneous Emergence of Multiple Drug Resistance in Tuberculosis before and during Therapy
Colijn, C., Cohen, T., Ganesh, A. J., & Murray, M. B. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018327 · Cited by 88
The Healing Factor: "Vitamin C" Against Disease
Stone, I. (1972) · Cited by 75
Beste, D. J. V., Peters, J., Hooper, T., Avignone-Rossa, C. A., Bushell, M. E., & McFadden, J. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.5.1677-1684.2005 · Cited by 75
Metaferia, B. B., Fetterolf, B., Shahzad‐ul‐Hussan, S., Moravec, M. D., Smith, J. A., Ray, S., Gutierrez‐Lugo, M., & Bewley, C. A. (2007) · Journal of Medicinal Chemistry · doi:10.1021/jm070669h · Cited by 74
Vilchèze, C., & Jacobs, W. R. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00832-12 · Cited by 71
Self-citationNAD+ auxotrophy is bacteriocidal for the tubercle bacilli
Vilchèze, C., Weinrick, B. C., Wong, K., Chen, B., & Jacobs, W. R. (2010) · Molecular Microbiology · doi:10.1111/j.1365-2958.2010.07099.x · Cited by 61
Self-citationVitamin C, the Common Cold and the Flu
Pauling, L. C. (1976) · Cited by 59
LANGLEY, K. E., Hawrot, E., & Kennedy, E. P. (1982) · Journal of Bacteriology · doi:10.1128/jb.152.3.1033-1041.1982 · Cited by 58
Selkon, J. B., Devadatta, S. C., Kulkarni, K. G., et al. (1964) · PubMed · Cited by 56
Waddell, S. J., Chung, G. A. C., Gibson, K. J. C., Everett, M. J., Minnikin, D. E., Besra, G. S., & Butcher, P. D. (2005) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2005.01659.x · Cited by 49
THE RELATION OF VITAMIN C DEFICIENCY TO INTESTINAL TUBERCULOSIS IN THE GUINEA PIG
McConkey, M., & Smith, D. T. (1933) · The Journal of Experimental Medicine · doi:10.1084/jem.58.4.503 · Cited by 39
Newton, G. L., Buchmeier, N. A., Clair, J. J. L., & Fahey, R. C. (2011) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2011.05.028 · Cited by 32
Cited by
Enzyme Mimicry for Combating Bacteria and Biofilms
Chen, Z., Wang, Z., Ren, J., & Qu, X. (2018) · Accounts of Chemical Research · doi:10.1021/acs.accounts.8b00011 · Cited by 499
A major role for ferroptosis in Mycobacterium tuberculosis –induced cell death and tissue necrosis
Amaral, E. P., Costa, D. L., Namasivayam, S., Riteau, N., Kamenyeva, O., Mittereder, L., Mayer-Barber, K. D., Andrade, B. B., & Sher, A. (2019) · Journal of Experimental Medicine · doi:10.1084/jem.20181776 · Cited by 464
Effects of metabolites derived from gut microbiota and hosts on pathogens
Li, Z., Quan, G., Jiang, X., Yang, Y., Ding, X., Zhang, D., Wang, X., Hardwidge, P. R., Ren, W., & Zhu, G. (2018) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2018.00314 · Cited by 157
Campos, A. I., & Zampieri, M. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.04.001 · Cited by 79
Chemistry and Redox Biology of Mycothiol
Reyes, A. M., Pedre, B., De Armas, M. I., Tossounian, M.-A., Radi, R., Messens, J., & Trujillo, M. (2018) · Antioxidants & Redox Signaling · doi:10.1089/ars.2017.7074 · Cited by 64
Metadata
- Authors
- Vilchèze, C., Hartman, T., Weinrick, B., & Jacobs, W. R.
- Venue
- Nature Communications
- Published
- 2013-05-21
- Volume
- 4
- Issue
- 1
- Pages
- 1881-1881
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2140867711
- Cited by
- 334
- References
- 40
- FWCI
- 14.36
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
