In vitro models for liver toxicity testing

Capturing complex 3D tissue physiology in vitro

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Toxicity Testing in the 21st Century: A Vision and a Strategy

Krewski, D., Acosta, D., Andersen, M., Anderson, H., Bailar, J. C., Boekelheide, K., Brent, R., Charnley, G., Cheung, V. G., Green, S., et al. (2010) · Journal of Toxicology and Environmental Health, Part B · doi:10.1080/10937404.2010.483176 · Cited by 1,688

The Liver: Biology and Pathobiology.

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Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells

Si-Tayeb, K., Noto, F. K., Nagaoka, M., Li, J., Battle, M. A., Duris, C., North, P. E., Dalton, S., & Duncan, S. A. (2010) · Hepatology · doi:10.1002/hep.23354 · Cited by 1,282

Toxicity testing in the 21st century: A vision and a strategy

Gibb, S. (2008) · Reproductive Toxicology · doi:10.1016/j.reprotox.2007.10.013 · Cited by 1,257

Microscale culture of human liver cells for drug development

Khetani, S. R., & Bhatia, S. N. (2008) · Nature Biotechnology · doi:10.1038/nbt1361 · Cited by 1,219

Induced Pluripotent Stem Cells and Embryonic Stem Cells Are Distinguished by Gene Expression Signatures

Chin, M. H., Mason, M. J., Xie, W., Volinia, S., Singer, M., Peterson, C., Ambartsumyan, G., Aimiuwu, O., Richter, L., Zhang, J., et al. (2009) · Cell Stem Cell · doi:10.1016/j.stem.2009.06.008 · Cited by 1,035

Effect of cell–cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and nonparenchymal cells

Bhatia, S. N., Balis, U. J., Yarmush, M. L., & Toner, M. (1999) · The FASEB Journal · doi:10.1096/fasebj.13.14.1883 · Cited by 890

Variation in the safety of induced pluripotent stem cell lines

Miura, K., Okada, Y., Aoi, T., Okada, A., Takahashi, K., Okita, K., Nakagawa, M., Koyanagi, M., Tanabe, K., Ohnuki, M., et al. (2009) · Nature Biotechnology · doi:10.1038/nbt.1554 · Cited by 877

Hepatocyte function and extracellular matrix geometry: long‐term culture in a sandwich configuration

Dunn, J. C. Y., Yarmush, M. L., Koebe, H. G., & Tompkins, R. G. (1989) · The FASEB Journal · doi:10.1096/fasebj.3.2.2914628 · Cited by 739

Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies

Hewitt, N. J., Gómez Lechón, M. J., Houston, J. B., Hallifax, D., Brown, H. S., Maurel, P., Kenna, J. G., Gustavsson, L., Lohmann, C., Skonberg, C., et al. (2007) · Drug Metabolism Reviews · doi:10.1080/03602530601093489 · Cited by 735

EXPRESSION OF CYTOCHROMES P450, CONJUGATING ENZYMES AND NUCLEAR RECEPTORS IN HUMAN HEPATOMA HepaRG CELLS

Aninat, C., Piton, A., Glaise, D., Le Charpentier, T., Langouët, S., Morel, F., Guguen-Guillouzo, C., & Guillouzo, A. (2006) · Drug Metabolism and Disposition · doi:10.1124/dmd.105.006759 · Cited by 703

Long‐Term in Vitro Function of Adult Hepatocytes in a Collagen Sandwich Configuration

Dunn, J. C. Y., Tompkins, R. G., & Yarmush, M. L. (1991) · Biotechnology Progress · doi:10.1021/bp00009a007 · Cited by 694

Directed differentiation of human embryonic stem cells into functional hepatic cells†

Cai, J., Zhao, Y., Liu, Y., Ye, F., Song, Z., Qin, H., Meng, S., Chen, Y., Zhou, R., Song, X., et al. (2007) · Hepatology · doi:10.1002/hep.21582 · Cited by 634

In Vitro Screening of Environmental Chemicals for Targeted Testing Prioritization: The ToxCast Project

Judson, R. S., Houck, K. A., Kavlock, R. J., Knudsen, T. B., Martin, M. T., Mortensen, H. M., Reif, D. M., Rotroff, D. M., Shah, I., Richard, A. M., et al. (2010) · Environmental Health Perspectives · doi:10.1289/ehp.0901392 · Cited by 608

Replacement of Diseased Mouse Liver by Hepatic Cell Transplantation

Rhim, J. A., Sandgren, E. P., Degen, J. L., Palmiter, R. D., & Brinster, R. L. (1994) · Science · doi:10.1126/science.8108734 · Cited by 577

A pilot study of ex vivo gene therapy for homozygous familial hypercholesterolaemia

Grossman, M., Rader, D. J., Muller, D. W. M., Kolansky, D. M., Kozarsky, K., Clark, B. J., Stein, E. A., Lupien, P. J., Brewer, H. B., Raper, S. E., et al. (1995) · Nature Medicine · doi:10.1038/nm1195-1148 · Cited by 530

The Toxicity Data Landscape for Environmental Chemicals

Judson, R., Richard, A., Dix, D. J., Houck, K., Martin, M., Kavlock, R., Dellarco, V., Henry, T., Holderman, T., Sayre, P., et al. (2009) · Environmental Health Perspectives · doi:10.1289/ehp.0800168 · Cited by 508

Generation of Functional Hepatocytes From Human Embryonic Stem Cells Under Chemically Defined Conditions That Recapitulate Liver Development

Touboul, T., Hannan, N. R. F., Corbineau, S., Martinez, A., Martinet, C., Branchereau, S., Mainot, S., Strick-Marchand, H., Pedersen, R., Di Santo, J., et al. (2010) · Hepatology · doi:10.1002/hep.23506 · Cited by 504

Human hepatocyte culture systems for the in vitro evaluation of cytochrome P450 expression and regulation

LeCluyse, E. L. (2001) · European Journal of Pharmaceutical Sciences · doi:10.1016/s0928-0987(01)00135-x · Cited by 477

Survival and Function of Hepatocytes on a Novel Three-Dimensional Synthetic Biodegradable Polymer Scaffold With an Intrinsic Network of Channels

Kim, S. S., Utsunomiya, H., Koski, J. A., Wu, B. M., Cima, M. J., Sohn, J., Mukai, K., Griffith, L. G., & Vacanti, J. P. (1998) · Annals of Surgery · doi:10.1097/00000658-199807000-00002 · Cited by 451

Perfused multiwell plate for 3D liver tissue engineering

Domansky, K., Inman, W., Serdy, J., Dash, A., Lim, M. H. M., & Griffith, L. G. (2010) · Lab Chip · doi:10.1039/b913221j · Cited by 444

Generation of Hepatocyte-Like Cells from Human Embryonic Stem Cells

Rambhatla, L., Chiu, C.-P., Kundu, P., Peng, Y., & Carpenter, M. K. (2003) · Cell Transplantation · doi:10.3727/000000003783985179 · Cited by 437

Hepatic maturation in differentiating embryonic stem cells in vitro

Hamazaki, T., Iiboshi, Y., Oka, M., Papst, P. J., Meacham, A. M., Zon, L. I., & Terada, N. (2001) · FEBS Letters · doi:10.1016/s0014-5793(01)02423-1 · Cited by 432

Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro

Baharvand, H., Hashemi, S. M., Kazemi Ashtiani, S., & Farrokhi, A. (2006) · The International Journal of Developmental Biology · doi:10.1387/ijdb.052072hb · Cited by 429

Efficient Differentiation of Functional Hepatocytes from Human Embryonic Stem Cells

Agarwal, S., Holton, K. L., & Lanza, R. (2008) · Stem Cells · doi:10.1634/stemcells.2007-1102 · Cited by 405

Liver cell models in in vitro toxicology.

Guillouzo, A. (1998) · Environmental Health Perspectives · doi:10.1289/ehp.98106511 · Cited by 385

Long-Term, Near-Total Liver Replacement by Transplantation of Isolated Hepatocytes in Rats Treated with Retrorsine

Laconi, E., Oren, R., Mukhopadhyay, D. K., Hurston, E., Laconi, S., Pani, P., Dabeva, M. D., & Shafritz, D. A. (1998) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)65574-5 · Cited by 381

The Design and Fabrication of Three-Chamber Microscale Cell Culture Analog Devices with Integrated Dissolved Oxygen Sensors

Sin, A., Chin, K. C., Jamil, M. F., Kostov, Y., Rao, G., & Shuler, M. L. (2008) · Biotechnology Progress · doi:10.1021/bp034077d · Cited by 368

A Microscale In Vitro Physiological Model of the Liver: Predictive Screens for Drug Metabolism and Enzyme Induction

Sivaraman, A., Leach, J., Townsend, S., Iida, T., Hogan, B., Stolz, D., Fry, R., Samson, L., Tannenbaum, S., & Griffith, L. (2005) · Current Drug Metabolism · doi:10.2174/138920005774832632 · Cited by 310

Transcriptional Signature and Memory Retention of Human-Induced Pluripotent Stem Cells

Marchetto, M. C. N., Yeo, G. W., Kainohana, O., Marsala, M., Gage, F. H., & Muotri, A. R. (2009) · PLoS ONE · doi:10.1371/journal.pone.0007076 · Cited by 310

Gene Expression in Two Hepatic Cell Lines, Cultured Primary Hepatocytes, and Liver Slices Compared to the in Vivo Liver Gene Expression in Rats: Possible Implications for Toxicogenomics Use of in Vitro Systems

Boess, F. (2003) · Toxicological Sciences · doi:10.1093/toxsci/kfg064 · Cited by 306

Microfabrication of Hepatocyte/Fibroblast Co-cultures: Role of Homotypic Cell Interactions

Bhatia, S. N., Balis, U. J., Yarmush, M. L., & Toner, M. (1998) · Biotechnology Progress · doi:10.1021/bp980036j · Cited by 304

Differentiation and isolation of hepatic-like cells from human embryonic stem cells

Lavon, N., Yanuka, O., & Benvenisty, N. (2004) · Differentiation · doi:10.1111/j.1432-0436.2004.07205002.x · Cited by 262

Generation of Endoderm-Derived Human Induced Pluripotent Stem Cells From Primary Hepatocytes

Liu, H., Ye, Z., Kim, Y., Sharkis, S., & Jang, Y.-Y. (2010) · Hepatology · doi:10.1002/hep.23626 · Cited by 250

General Review on In Vitro Hepatocyte Models and Their Applications

Guguen-Guillouzo, C., & Guillouzo, A. (2010) · Methods in Molecular Biology · doi:10.1007/978-1-60761-688-7_1 · Cited by 239

In Vitro Zonation and Toxicity in a Hepatocyte Bioreactor

Allen, J. W. (2005) · Toxicological Sciences · doi:10.1093/toxsci/kfi052 · Cited by 233

Correlation of Biliary Excretion in Sandwich-Cultured Rat Hepatocytes and In Vivo in Rats

Liu, X., Chism, J. P., LeCluyse, E. L., Brouwer, K. R., & Brouwer, K. L. R. (1999) · Drug Metabolism and Disposition · doi:10.1016/s0090-9556(24)15299-3 · Cited by 228

Immune interactions in endometriosis

Herington, J. L., Bruner-Tran, K. L., Lucas, J. A., & Osteen, K. G. (2011) · Expert Review of Clinical Immunology · doi:10.1586/eci.11.53 · Cited by 228

HepaRG human hepatic cell line utility as a surrogate for primary human hepatocytes in drug metabolism assessment in vitro

Lübberstedt, M., Müller-Vieira, U., Mayer, M., Biemel, K. M., Knöspel, F., Knobeloch, D., Nüssler, A. K., Gerlach, J. C., & Zeilinger, K. (2011) · Journal of Pharmacological and Toxicological Methods · doi:10.1016/j.vascn.2010.04.013 · Cited by 227

Modulation of functional activities in cultured rat hepatocytes

Guguen-Guillouzo, C., & Guillouzo, A. (1983) · Molecular and Cellular Biochemistry · doi:10.1007/bf00225245 · Cited by 217

A microfluidic hepatic coculture platform for cell-based drug metabolism studies

Novik, E., Maguire, T. J., Chao, P., Cheng, K. C., & Yarmush, M. L. (2010) · Biochemical Pharmacology · doi:10.1016/j.bcp.2009.11.010 · Cited by 212

Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes

LeCluyse, E. L., Bullock, P. L., & Parkinson, A. (1996) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(96)00418-8 · Cited by 193

Amelioration of radiation-induced liver damage in partially hepatectomized rats by hepatocyte transplantation.

Guha, C., Sharma, A., Gupta, S. K., Alfieri, A. A., Gorla, G. R., Gagandeep, S., Sokhi, R. P., Roy-Chowdhury, N., Tanaka, K. E., Vikram, B., et al. (1999) · PubMed · Cited by 189

Precision-cut liver slices: characteristics and use for in vitro pharmaco-toxicology

Lerche-Langrand, C., & Toutain, H. J. (2000) · Toxicology · doi:10.1016/s0300-483x(00)00316-4 · Cited by 188

In Vitro Organogenesis of Liver Tissuea

GRIFFITH, L. G., WU, B., CIMA, M. J., POWERS, M. J., CHAIGNAUD, B., & VACANTI, J. P. (1997) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1997.tb52212.x · Cited by 170

Differentiation and Characterization of Metabolically Functioning Hepatocytes from Human Embryonic Stem Cells

Duan, Y., Ma, X., Zou, W., Wang, C., Bahbahan, I. S., Ahuja, T. P., Tolstikov, V., & Zern, M. A. (2010) · Stem Cells · doi:10.1002/stem.315 · Cited by 169

Evaluation of a microfluidic based cell culture platform with primary human hepatocytes for the prediction of hepatic clearance in human

Chao, P., Maguire, T., Novik, E., Cheng, K.-C., & Yarmush, M. L. (2009) · Biochemical Pharmacology · doi:10.1016/j.bcp.2009.05.013 · Cited by 161

Liver tissue engineering in the evaluation of drug safety

Dash, A., Inman, W., Hoffmaster, K., Sevidal, S., Kelly, J., Obach, R. S., Griffith, L. G., & Tannenbaum, S. R. (2009) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425250903160664 · Cited by 158

The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies

Marion, M., Hantz, O., & Durantel, D. (2010) · Methods in molecular biology · doi:10.1007/978-1-60761-688-7_13 · Cited by 154

Prolonged Maintenance of Active Cytochrome P-450 in Adult Rat Hepatocytes Co-Cultured with Another Liver Cell Type

Bégué, J., Guguen‐Guillouzo, C., Pasdeloup, N., & Guillouzo, A. (1984) · Hepatology · doi:10.1002/hep.1840040507 · Cited by 151

Evolving concepts in liver tissue modeling and implications forin vitrotoxicology

Guillouzo, A., & Guguen‐Guillouzo, C. (2008) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425255.4.10.1279 · Cited by 151

Assessment of a Micropatterned Hepatocyte Coculture System to Generate Major Human Excretory and Circulating Drug Metabolites

Wang, W. W., Khetani, S. R., Krzyzewski, S., Duignan, D. B., & Obach, R. S. (2010) · Drug Metabolism and Disposition · doi:10.1124/dmd.110.034876 · Cited by 151

Differentiation of Human and Mouse Embryonic Stem Cells along a Hepatocyte Lineage

Shirahashi, H., Wu, J., Yamamoto, N., Catana, A. M., Wege, H., Wager, B., Okita, K., & Zern, M. Α. (2004) · Cell Transplantation · doi:10.3727/000000004783984016 · Cited by 149

Efficient assembly of rat hepatocyte spheroids for tissue engineering applications

Wu, F. J., Friend, J. R., Hsiao, C. C., Zilliox, M. J., Ko, W., Cerra, F. B., & Hu, W. (1996) · Biotechnology and Bioengineering · Cited by 141

Defined Conditions for Development of Functional Hepatic Cells from Human Embryonic Stem Cells

Schwartz, R. E., Linehan, J. L., Painschab, M., Hu, W., Verfaillie, C. M., & Kaufman, D. S. (2005) · Stem Cells and Development · doi:10.1089/scd.2005.14.643 · Cited by 138

Three-dimensional culture of hepatocytes for prediction of drug-induced hepatotoxicity

Meng, Q. (2010) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425251003674356 · Cited by 138

Activity profiles of 309 ToxCast™ chemicals evaluated across 292 biochemical targets

Knudsen, T. B., Houck, K. A., Sipes, N. S., Singh, A. V., Judson, R., Martin, M. T., Weissman, A. D., Kleinstreuer, N., Mortensen, H. M., Reif, D. M., et al. (2011) · Toxicology · doi:10.1016/j.tox.2010.12.010 · Cited by 134

Synergistic drug–cytokine induction of hepatocellular death as an in vitro approach for the study of inflammation-associated idiosyncratic drug hepatotoxicity

Cosgrove, B. D., King, B. M., Hasan, M. A., Alexopoulos, L. G., Farazi, P. A., Hendriks, B. S., Griffith, L. G., Sorger, P. K., Tidor, B., Xu, J. J., et al. (2009) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2009.04.002 · Cited by 134

The Liver: Biology and Pathobiology

Iber, F. L. (2002) · JAMA · doi:10.1001/jama.287.4.524 · Cited by 128

Hepatic tissue engineering for adjunct and temporary liver support: Critical technologies

Chan, C., Francois Berthiaume, B., Nath, B., Tilles, A. W., Toner, M., & Yarmush, M. L. (2004) · Liver Transplantation · doi:10.1002/lt.20229 · Cited by 125

Hepatocyte-like cells derived from human embryonic stem cells specifically via definitive endoderm and a progenitor stage

Brolén, G., Sivertsson, L., Björquist, P., Eriksson, G., Ek, M., Semb, H., Johansson, I., Andersson, T. B., Ingelman‐Sundberg, M., & Heins, N. (2009) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2009.11.007 · Cited by 124

Idiosyncratic Drug-Induced Liver Injury and the Role of Inflammatory Stress with an Emphasis on an Animal Model of Trovafloxacin Hepatotoxicity

Shaw, P. J., Ganey, P. E., & Roth, R. A. (2010) · Toxicological Sciences · doi:10.1093/toxsci/kfq168 · Cited by 123

Exploring interactions between rat hepatocytes and nonparenchymal cells using gene expression profiling

Khetani, S. R., Szulgit, G. K., Rio, J. A. D., Barlow, C., & Bhatia, S. N. (2004) · Hepatology · doi:10.1002/hep.20351 · Cited by 122

Long-Term Culture of Functional Liver Tissue: Three-Dimensional Coculture of Primary Hepatocytes and Stellate Cells

Riccalton-Banks, L., Liew, C., Bhandari, R., Fry, J. R., & Shakesheff, K. M. (2003) · Tissue Engineering · doi:10.1089/107632703322066589 · Cited by 119

Scaling Down of a Clinical Three-Dimensional Perfusion Multicompartment Hollow Fiber Liver Bioreactor Developed for Extracorporeal Liver Support to an Analytical Scale Device Useful for Hepatic Pharmacological In Vitro Studies

Zeilinger, K., Schreiter, T., Darnell, M., Söderdahl, T., Lübberstedt, M., Dillnér, B., Knobeloch, D., Nussler, A. K., Gerlach, J. C., & Andersson, T. B. (2011) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2010.0580 · Cited by 118

Layered Patterning of Hepatocytes in Co-Culture Systems using Microfabricated Stencils

Cho, C. H., Park, J., Tilles, A. W., Francois Berthiaume, B., Toner, M., & Yarmush, M. L. (2010) · BioTechniques · doi:10.2144/000113317 · Cited by 117

The current status of primary hepatocyte culture

Mitaka, T. (1998) · International Journal of Experimental Pathology · doi:10.1046/j.1365-2613.1998.00083.x · Cited by 115

Rat liver sinusoidal endothelial cells survive without exogenous VEGF in 3D perfused co‐cultures with hepatocytes

Hwa, A. J., Fry, R. C., Sivaraman, A., So, P. T. C., Samson, L. D., Stolz, D. B., & Griffith, L. G. (2007) · The FASEB Journal · doi:10.1096/fj.06-7473com · Cited by 110

3D hepatocyte monolayer on hybrid RGD/galactose substratum

Du, Y., Chia, S., Han, R., Chang, S., Tang, H., & Yu, H. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.07.018 · Cited by 105

Comparison of five incubation systems for rat liver slices using functional and viability parameters

Olinga, P., Groen, K., Hof, I. H., de Kanter, R., Koster, H. J., Leeman, W. R., Rutten, A. A. J. J. L., van Twillert, K., & Groothuis, G. M. M. (1997) · Journal of Pharmacological and Toxicological Methods · doi:10.1016/s1056-8719(97)00060-9 · Cited by 102

Stem cell-derived hepatocytes and their use in toxicology

Guguen‐Guillouzo, C., Corlu, A., & Guillouzo, A. (2009) · Toxicology · doi:10.1016/j.tox.2009.09.019 · Cited by 98

Maintaining hepatocyte differentiation in vitro through co-culture with hepatic stellate cells

Krause, P., Saghatolislam, F., Koenig, S., Unthan‐Fechner, K., & Probst, I. (2009) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-008-9166-1 · Cited by 97

Modeling inflammation–drug interactions in vitro: A rat Kupffer cell-hepatocyte coculture system

Tukov, F. F., Maddox, J. F., Amacher, D. E., Bobrowski, W. F., Roth, R. A., & Ganey, P. E. (2006) · Toxicology in Vitro · doi:10.1016/j.tiv.2006.04.005 · Cited by 88

P450 enzyme expression patterns in the NCI human tumor cell line panel.

Yu, L. J., Matias, J., Scudiero, D. A., Hite, K. M., Monks, A. P., Sausville, E. A., & Waxman, D. J. (2001) · PubMed · Cited by 86

A Stable Long‐Term Hepatocyte Culture System for Studies of Physiologic Processes: Cytokine Stimulation of the Acute Phase Response in Rat and Human Hepatocytes

Bader, A., Rinkes, I. H. M. B., Closs, E. I., Ryan, C. M., Toner, M., Cunningham, J. M., Tompkins, R. G., & Yarmush, M. L. (1992) · Biotechnology Progress · doi:10.1021/bp00015a007 · Cited by 85

Formation of plasma membrane domains in rat hepatocytes and hepatoma cell lines in culture

Maurice, M., Rogier, E., Cassio, D., & Feldmann, G. (1988) · Journal of Cell Science · doi:10.1242/jcs.90.1.79 · Cited by 83

Chitosan nanofiber scaffold enhances hepatocyte adhesion and function

Chu, X.-H., Shi, X., Feng, Z., Gu, Z., & Ding, Y. (2008) · Biotechnology Letters · doi:10.1007/s10529-008-9892-1 · Cited by 80

Regulation of Hepatic Nitric Oxide Synthase by Reactive Oxygen Intermediates and Glutathione

Duval, D. L., Sieg, D. J., & Billings, R. E. (1995) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1995.1093 · Cited by 77

Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes

Mathijs, K., Kienhuis, A. S., Brauers, K. J. J., Jennen, D. G. J., Lahoz, A., Kleinjans, J. C. S., & van Delft, J. H. M. (2009) · Drug Metabolism and Disposition · doi:10.1124/dmd.108.025775 · Cited by 74

Three-Dimensional Perfusion Bioreactor Culture Supports Differentiation of Human Fetal Liver Cells

Schmelzer, E., Triolo, F., Turner, M. E., Thompson, R. L., Zeilinger, K., Reid, L. M., Gridelli, B. G., & Gerlach, J. C. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0569 · Cited by 70

Evidence that the loss of rat liver cytochrome P450 in vitro is not solely associated with the use of collagenase, the loss of cell-cell contacts and/or the absence of an extracellular matrix

Wright, M. C., & Paine, A. J. (1992) · Biochemical Pharmacology · doi:10.1016/0006-2952(92)90283-o · Cited by 67

PREDICTION OF IN VITRO INTRINSIC CLEARANCE FROM HEPATOCYTES: COMPARISON OF SUSPENSIONS AND MONOLAYER CULTURES

Griffin, S. J., & Houston, J. B. (2005) · Drug Metabolism and Disposition · doi:10.1124/dmd.33.1.115 · Cited by 67

Gene expression analysis of precision-cut human liver slices indicates stable expression of ADME-Tox related genes

Elferink, M. G. L., Olinga, P., van Leeuwen, E. M., Bauerschmidt, S., Polman, J., Schoonen, W. G. E. J., Heisterkamp, S. H., & Groothuis, G. M. M. (2011) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2011.03.010 · Cited by 65

Working in partnership to advance the 3Rs in toxicity testing

Holmes, A. M., Creton, S. K., & Chapman, K. L. (2009) · Toxicology · doi:10.1016/j.tox.2009.11.006 · Cited by 62

Bioengineering of liver assist devices

Tilles, A. W., Berthiaume, F., Yarmush, M. L., Tompkins, R. G., & Toner, M. (2002) · Journal of Hepato-Biliary-Pancreatic Surgery · doi:10.1007/s005340200095 · Cited by 59

Cytokine-associated drug toxicity in human hepatocytes is associated signaling network dysregulation

Hendriks, B. S., Cosgrove, B. D., Alexopoulos, L. G., Hang, T.-C., Sorger, P. K., Griffith, L. G., & Lauffenburger, D. A. (2009) · DSpace@MIT (Massachusetts Institute of Technology) · Cited by 59

Primary culture of rat hepatocytes in 96-well plates: Effects of extracellular matrix configuration on cytochrome P450 enzyme activity and inducibility, and its application in in vitro cytotoxicity screening

Mingoia, R. T., Nabb, D. L., Yang, C., & Han, X. (2006) · Toxicology in Vitro · doi:10.1016/j.tiv.2006.10.012 · Cited by 58

Quantitative differences in phase I and II metabolism between rat precision-cut liver slices and isolated hepatocytes.

Ekins, S., Murray, G. I., Burke, M. D., Williams, J. A., Marchant, N., & HAWKSWORTH, G. M. (1995) · Drug Metabolism and Disposition · doi:10.1016/s0090-9556(25)06827-8 · Cited by 57

Co-culture of hepatocytes and fibroblasts by micropatterned immobilization of β-galactose derivatives

Kang, I., Kim, G. J., Kwon, O. H., & Ito, Y. (2003) · Biomaterials · doi:10.1016/j.biomaterials.2003.11.004 · Cited by 55

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