Simultaneous dopamine and single-unit recordings reveal accumbens GABAergic responses: implications for intracranial self-stimulation
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Intracranial self-stimulation (ICS) is a motivated behavior that results from contingent activation of the brain reward system. ICS with stimulating electrodes placed in the medial forebrain bundle (MFB) is particularly robust. However, the neurons that course through this pathway use a variety of neurotransmitters including dopamine and GABA. For this reason, the neurotransmitters that are central to this behavior, and the specific roles that they subserve, remain unclear. Here, we used extracellular electrophysiology and cyclic voltammetry at the same electrode in awake rats to simultaneously examine cell firing and dopamine release in the nucleus accumbens (NAc) during ICS and noncontingent stimulation of the MFB. ICS elicited dopamine release in the NAc and produced coincident time-locked changes (predominantly inhibitions) in the activity of a subset of NAc neurons. Similar responses were elicited with noncontingent stimulations. The changes in firing rate induced by noncontingent stimulations were reversed by the GABA(A) receptor antagonist bicuculline. Most time-locked unit activity was unaffected by D1 or D2-like dopamine-receptor antagonists, or by inhibition of evoked dopamine release, although, for a minority of units, the D1 dopamine-receptor antagonist SCH23390 attenuated neural activity. Thus, neurons in the NAc are preferentially inhibited by GABA(A) receptors after MFB stimulation, a mechanism that may also be important in ICS.
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Electrical brain stimulation at the medial forebrain bundle/VTA is rewarding and triggers dopamine release in the ventral striatum and investigatory responses of sniffing.
“Electrical brain stimulation at the medial forebrain bundle/VTA is rewarding and triggers dopamine release in the ventral striatum (Fiorino et al., 1993; Garris et al., 1999; Cheer et al., 2005; Hernandez et al., 2006) and investigatory responses of sniffing (Clarke and Trowill, 1971; Ikemoto and Panksepp, 1994).”
From Dopamine reward circuitry: Two projection systems from the ventral midbrain to the nucleus accumbens–olfactory tubercle complex (2007) · cites this paper as Cheer et al., 2005
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“, 2012), and often decreases the activity of VS medium spiny neurons (Peoples and West, 1996; Cheer et al., 2005; Roitman et al., 2005; Wheeler and Carelli, 2009).”
BackgroundKroemer, N. B., Guevara, A., Ciocanea Teodorescu, I., Wuttig, F., Kobiella, A., & Smolka, M. N. (2014) · NeuroImage · doi:10.1016/j.neuroimage.2014.07.060 · Cited by 64 · see citing works
- Authors
- Cheer, J. F., Heien, M. L. A. V., Garris, P. A., Carelli, R. M., & Wightman, R. M.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2005-12-27
- Volume
- 102
- Issue
- 52
- Pages
- 19150-19155
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC1323210
- OpenAlex
- W2027852160
- Cited by
- 143
- References
- 55
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 3 · 2022: 2 · 2021: 6 · 2020: 5 · 2019: 11 · 2018: 3 · 2017: 7 · 2016: 9 · 2015: 12 · 2014: 9 · 2013: 14 · 2012: 4
- FWCI
- 3.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Carolina Digital Repository (University of North Carolina at Chapel Hill) (submittedVersion)
- UNC Libraries
- Funders
- U.S. Public Health Service (017318, 10900)
- Medical Research and Materiel Command
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Neurology › Neurological disorders and treatments
- Keywords
- intracranial self-stimulation, nucleus accumbens, GABAergic neurons, dopamine release, medial forebrain bundle, GABAA receptor, single-unit activity, dopamine D1 receptor, dopamine D2 receptor, reward systems, bicuculline, SCH 23390
- MeSH terms
- Animals, Benzazepines, Bicuculline, Dopamine, Electrodes, Electrophysiology, Evoked Potentials, Male, Neurons, Time Factors, Rats, Sprague-Dawley, Receptors, Dopamine D1, Neurotransmitter Agents, Dopamine Antagonists, GABA Modulators, Rats
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