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A highly conserved repetitive DNA sequence,(TTAGGG) n, present at the telomeres of human chromosomes

Moyzis, R K, Buckingham, J M, Cram, L S, Dani, M, Deaven, L L, Jones, M D, Meyne, J, Ratliff, R L, Wu, J R·1988·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.85.18.6622
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Abstract

A highly conserved repetitive DNA sequence, (TTAGGG)n, has been isolated from a human recombinant repetitive DNA library. Quantitative hybridization to chromosomes sorted by flow cytometry indicates that comparable amounts of this sequence are present on each human chromosome. Both fluorescent in situ hybridization and BAL-31 nuclease digestion experiments reveal major clusters of this sequence at the telomeres of all human chromosomes. The evolutionary conservation of this DNA sequence, its terminal chromosomal location in a variety of higher eukaryotes (regardless of chromosome number or chromosome length), and its similarity to functional telomeres isolated from lower eukaryotes suggest that this sequence is a functional human telomere.

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Cited by

Metadata

Authors
Moyzis, R. K., Buckingham, J. M., Cram, L. S., Dani, M., Deaven, L. L., Jones, M. D., Meyne, J., Ratliff, R. L., & Wu, J. R.
Affiliations
Genetics Group, LS-3, Los Alamos National Laboratory, University of California 87545.
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1988-09
Volume
85
Issue
18
Pages
6622-6626
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.85.18.6622
Cited by
1,820
References
1
Language
en

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