Cited by 282Lipid metabolism and biosynthesisCancer, Lipids, and MetabolismPeroxisome Proliferator-Activated Receptors

Increased Rates of Fatty Acid Uptake and Plasmalemmal Fatty Acid Transporters in Obese Zucker Rats

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Luiken, Joost J.F.P., Arumugam, Yoga, Dyck, David J., Bell, Rhonda C., Pelsers, Maurice M.L., Turcotte, Lorraine P., Tandon, Narendra N., Glatz, Jan F.C. +1 more·2001·Journal of Biological Chemistry

Abstract

Giant vesicles were used to study the rates of uptake of long-chain fatty acids by heart, skeletal muscle, and adipose tissue of obese and lean Zucker rats. With obesity there was an increase in vesicular fatty acid uptake of 1.8-fold in heart, muscle and adipose tissue. In some tissues only fatty acid translocase (FAT) mRNA (heart, +37%; adipose, +80%) and fatty acid-binding protein (FABPpm) mRNA (heart, +148%; adipose, +196%) were increased. At the protein level FABPpm expression was not changed in any tissues except muscle (+14%), and FAT/CD36 protein content was altered slightly in adipose tissue (+26%). In marked contrast, the plasma membrane FAT/CD36 protein was increased in heart (+60%), muscle (+80%), and adipose tissue (+50%). The plasma membrane FABPpm was altered only in heart (+50%) and adipose tissues (+70%). Thus, in obesity, alterations in fatty acid transport in metabolically important tissues are not associated with changes in fatty acid transporter mRNAs or altered fatty acid transport protein expression but with their increased abundance at the plasma membrane. We speculate that in obesity fatty acid transporters are relocated from an intracellular pool to the plasma membrane in heart, muscle, and adipose tissues.

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Crossref · 10.1074/jbc.m100052200

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  • CD36 has an important role in long-chain fatty acid uptake into skeletal muscle in rodents and humans, and its concentration and activity increase after exercise.

    “There is abundant evidence which indicates that CD36 plays an important role in LCFA uptake into skeletal muscle in rodents and human and its concentration and activity are increased following exercise [89–96].”

    From Pathogenesis of insulin resistance in skeletal muscle (2010) · cites this paper as [92]

  • Increased plasmalemma skeletal muscle CD36 is associated with increased FFA uptake and insulin resistance in animal models and obese and diabetic human subjects.

    “The potential importance of CD36 in regulating insulin sensitivity is supported by studies conducted in animal models and in obese and diabetic human subjects that found increased plasmalemma skeletal muscle CD36 was associated with increased FFA uptake and insulin resistance (25,30,31)”

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Authors
Luiken, J. J. F. P., Arumugam, Y., Dyck, D. J., Bell, R. C., Pelsers, M. M. L., Turcotte, L. P., Tandon, N. N., Glatz, J. F. C., & Bonen, A.
Affiliations
  • University of Waterloo (CA)
  • University of Guelph (CA)
  • University of Alberta (CA)
  • University of Southern California (US)
  • Maastricht University (NL)
Corresponding author
Bonen, Arend
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-11
Volume
276
Issue
44
Pages
40567-40573
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2069262764
Cited by
282
References
42
Citations by year
2026: 1 · 2025: 4 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 17 · 2020: 10 · 2019: 5 · 2018: 6 · 2017: 6 · 2016: 10 · 2015: 5 · 2014: 7 · 2013: 14 · 2012: 14
FWCI
8.37
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r29ar045168-04)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR 45168)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords
fatty acid uptake, obesity, Zucker rats, fatty acid translocase, FAT/CD36, fatty acid binding protein, plasma membrane localization, heart, skeletal muscle, adipose tissue, vesicular uptake
MeSH terms
Animals, Fatty Acids, Female, Kinetics, Membrane Glycoproteins, Obesity, Rats, Zucker, RNA, Messenger, CD36 Antigens, Organic Anion Transporters, Rats

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