Cited by 164Antifungal resistance and susceptibilityYeasts and Rust Fungi StudiesFungal and yeast genetics research

Confirmation of fifth Candida auris clade by whole genome sequencing

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Spruijtenburg, Bram, Badali, Hamid, Abastabar, Mahdi, Mirhendi, Hossein, Khodavaisy, Sadegh, Sharifisooraki, Joobin, Taghizadeh Armaki, Mojtaba, de Groot, Theun +1 more·2022·Emerging Microbes & Infections

Abstract

Candida auris has emerged globally as a multidrug-resistant pathogen causing outbreaks in health care facilities. Whole genome sequencing (WGS) analysis has identified four major clades, while earlier WGS data from a single Iranian isolate suggested the existence of a potential fifth clade. Here, we confirm the existence of this fifth clade by providing WGS data of another four Iranian isolates. These clade V isolates differed less than 100 single-nucleotide polymorphisms (SNPs) between each other, while they were separated from the other clades by more than 200,000 SNPs. Two of these isolates were resistant to fluconazole and were found to harbour mutations in the TAC1b and ERG11 genes.

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  1. [34]CLSI. Reference method for broth dilution antifungal susceptibility testing of yeasts: Approved Standard 3rd Edition. CLSI Document M27-A3. Clinical and Laboratory Standards Institute Wayne, PA; 2008

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Authors
Spruijtenburg, B., Badali, H., Abastabar, M., Mirhendi, H., Khodavaisy, S., Sharifisooraki, J., Taghizadeh Armaki, M., de Groot, T., & Meis, J. F.
Affiliations
  • Radboud University Medical Center (NL)
  • The University of Texas at San Antonio (US)
  • Mazandaran University of Medical Sciences (IR)
  • Isfahan University of Medical Sciences (IR)
  • Tehran University of Medical Sciences (IR)
  • Islamic Azad University, Tehran (IR)
  • Babol University of Medical Sciences
  • Canisius Wilhelmina Hospital
Corresponding author
Meis, Jacques F.
Venue
Emerging Microbes & Infections
Published
2022-10-18
Volume
11
Issue
1
Pages
2405-2411
ISSN
2222-1751 (ISSN-L)
OpenAlex
W4297229549
Cited by
164
References
44
Citations by year
2026: 37 · 2025: 30 · 2024: 57 · 2023: 39 · 2022: 1
FWCI
15.49
Type
journal article
Language
en
Open access
Locations
  • Emerging Microbes & Infections (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Radboud Repository (Radboud University) (submittedVersion)
  • Radboud Repository (Radboud University) (submittedVersion, other-oa)
Funders
Canisius Wilhelmina Hospital
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
Candida auris, fungal clades, multidrug resistance, fluconazole resistance, ERG11 mutations, single nucleotide polymorphisms, Iranian isolates, whole genome sequencing
MeSH terms
Whole Genome Sequencing, Candida auris, Antifungal Agents, Candida, Candidiasis, Humans, Iran, Microbial Sensitivity Tests, Fluconazole, Polymorphism, Single Nucleotide

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