Cited by 253Neuroscience and Neuropharmacology ResearchMemory and Neural MechanismsNeurotransmitter Receptor Influence on Behavior

Dopaminergic stimulation of local protein synthesis enhances surface expression of GluR1 and synaptic transmission in hippocampal neurons

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Smith, W. Bryan, Starck, Shelley R., Roberts, Richard W., Schuman, Erin M.·2005·Neuron

Abstract

The use-dependent modification of synapses is strongly influenced by dopamine, a transmitter that participates in both the physiology and pathophysiology of animal behavior. In the hippocampus, dopaminergic signaling is thought to play a key role in protein synthesis-dependent forms of synaptic plasticity. The molecular mechanisms by which dopamine influences synaptic function, however, are not well understood. Using a GFP-based reporter, as well as a small-molecule reporter of endogenous protein synthesis, we show that dopamine D1/D5 receptor activation stimulates local protein synthesis in the dendrites of hippocampal neurons. We also identify the GluR1 subunit of AMPA receptors as one protein upregulated by dopamine receptor activation, with increased incorporation of surface GluR1 at synaptic sites. The insertion of new GluRs is accompanied by an increase in the frequency of miniature synaptic events. Together, these data suggest a local protein synthesis-dependent activation of previously silent synapses as a result of dopamine receptor stimulation.

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Crossref · 10.1016/j.neuron.2005.01.015

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Authors
Smith, W. B., Starck, S. R., Roberts, R. W., & Schuman, E. M.
Affiliations
  • Howard Hughes Medical Institute (US)
  • California Institute of Technology (US)
Corresponding author
Schuman, Erin M.
Venue
Neuron
Publisher
Elsevier BV
Published
2005-03
Volume
45
Issue
5
Pages
765-779
ISSN
0896-6273 (ISSN-L)
OpenAlex
W2091597912
Cited by
252
References
63
Citations by year
2026: 4 · 2025: 12 · 2024: 10 · 2023: 10 · 2022: 3 · 2021: 7 · 2020: 7 · 2019: 7 · 2018: 9 · 2017: 8 · 2016: 7 · 2015: 12 · 2014: 10 · 2013: 19 · 2012: 17
FWCI
8.04
Type
journal article
Language
en
Open access
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
Keywords
dopamine, local protein synthesis, GluR1, AMPA receptors, synaptic plasticity, hippocampal neurons, dendritic protein synthesis, cell surface receptor expression, silent synapses, synaptic transmission
MeSH terms
Animals, Cells, Cultured, Gene Expression Regulation, Hippocampus, Synaptic Transmission, Neurons, Proteins, Receptors, Dopamine, Synapses, Rats, Sprague-Dawley, Receptors, AMPA, Dopamine Agonists, Rats

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