Brevican-containing perineuronal nets of extracellular matrix in dissociated hippocampal primary cultures

John, Nora; Krügel, Hans; Frischknecht, Renato; Smalla, Karl-Heinz; Schultz, Christian; Kreutz, Michael R.; Gundelfinger, Eckart D.; Seidenbecher, Constanze I.

doi:10.1016/j.mcn.2006.01.011

Cited by 122Proteoglycans and glycosaminoglycans researchNeuroscience and Neuropharmacology ResearchNeurogenesis and neuroplasticity mechanisms

Brevican-containing perineuronal nets of extracellular matrix in dissociated hippocampal primary cultures

John, Nora, Krügel, Hans, Frischknecht, Renato, Smalla, Karl-Heinz, Schultz, Christian, Kreutz, Michael R., Gundelfinger, Eckart D., Seidenbecher, Constanze I.·2006·Molecular and Cellular Neuroscience

DOI 10.1016/j.mcn.2006.01.011

Open this paper in CoChat — free account

Abstract

Perineuronal nets (PNN) are specialized extracellular matrix structures enwrapping CNS neurons, which are important regulators for neuronal and synaptic functions. Brevican, a chondroitin sulfate proteoglycan, is an integral component of PNN. Here, we have investigated the appearance of these structures in hippocampal primary cultures. The expression profile of brevican in mixed cultures resembles the in vivo pattern with a strong upregulation of all isoforms during the second and 3rd weeks in culture. Brevican is primarily synthesized by co-cultured glial fibrillary acidic protein (GFAP-)-positive astrocytes and co-assembles with its interaction partners in PNN-like structures on neuronal somata and neurites as identified by counterstaining with the PNN marker Vicia villosa lectin. Both excitatory and inhibitory synapses are embedded into PNN. Furthermore, axon initial segments are strongly covered by a dense brevican coat. Altogether, we show that mature primary cultures can form PNN, and that basic features of these extracellular matrix structures may be studied in vitro.

Integrity signals

Registry

Crossref · 10.1016/j.mcn.2006.01.011

Cites

54 of 54 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The ECM in the AIS seems to be mainly comprised of brevican.
“However, the ECM seems to be mainly comprised of brevican (John et al., 2006).”
From The hyaluronan and proteoglycan link proteins: Organizers of the brain extracellular matrix and key molecules for neuronal function and plasticity (2015) · cites this paper as John et al., 2006
Brevican enrichment in the AIS is mediated via direct interaction with NF186, which is diminished by knockdown of NF186.
“Brevican enrichment in the AIS was mediated via direct interaction with NF186, which was diminished by a knockdown of NF186 (John et al., 2006;Hedstrom et al., 2007).”
From The hyaluronan and proteoglycan link proteins: Organizers of the brain extracellular matrix and key molecules for neuronal function and plasticity (2015) · cites this paper as John et al., 2006

From Ionic Currents to Molecular Mechanisms
Catterall, W. A. (2000) · Neuron · doi:10.1016/s0896-6273(00)81133-2 · Cited by 2,124 · see citing works
Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues.
Bennett, V., & Baines, A. (2001) · Physiological Reviews · doi:10.1152/physrev.2001.81.3.1353 · Cited by 993 · see citing works
Proteoglycans of the extracellular environment: clues from the gene and protein side offer novel perspectives in molecular diversity and function
Iozzo, R. V., & Murdoch, A. D. (1996) · The FASEB Journal · doi:10.1096/fasebj.10.5.8621059 · Cited by 647 · see citing works
Lecticans: organizers of the brain extracellular matrix
Yamaguchi, Y. (2000) · Cellular and Molecular Life Sciences · doi:10.1007/pl00000690 · Cited by 642 · see citing works
Extracellular matrix molecules and synaptic plasticity
Dityatev, A., & Schachner, M. (2003) · Nature Reviews Neuroscience · doi:10.1038/nrn1115 · Cited by 564 · see citing works
Perineuronal nets: past and present
Celio, M. R., Spreafico, R., De Biasi, S., & Vitellaro-Zuccarello, L. (1998) · Trends in Neurosciences · doi:10.1016/s0166-2236(98)01298-3 · Cited by 497 · see citing works
Bassoon, a novel zinc-finger CAG/glutamine-repeat protein selectively localized at the active zone of presynaptic nerve terminals
Dieck, S., Sanmartí-Vila, L., Langnaese, K., Richter, K., Kindler, S., Soyke, A., Wex, H., Smalla, K.-H., Kämpf, U., Fränzer, J.-T., et al. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.142.2.499 · Cited by 482 · see citing works
Self-citation
Brain extracellular matrix
Ruoslahti, E. (1996) · Glycobiology · doi:10.1093/glycob/6.5.489 · Cited by 467 · see citing works
The Presynaptic Particle Web
Phillips, G. R., Huang, J. K., Wang, Y., Tanaka, H., Shapiro, L. S., Zhang, W., Shan, W., Arndt, K., Frank, M., Gordon, R. E., et al. (2001) · Neuron · doi:10.1016/s0896-6273(01)00450-0 · Cited by 438 · see citing works
Golgi-disturbing agents
Dinter, A., & Berger, E. G. (1998) · Histochemistry and Cell Biology · doi:10.1007/s004180050256 · Cited by 389 · see citing works
Perineuronal nets — a specialized form of extracellular matrix in the adult nervous system
Celio, M. R., & Blümcke, I. (1994) · Brain Research Reviews · doi:10.1016/0165-0173(94)90006-x · Cited by 386 · see citing works
Ankyrin-Based Subcellular Gradient of Neurofascin, an Immunoglobulin Family Protein, Directs GABAergic Innervation at Purkinje Axon Initial Segment
Ango, F., Cristo, G. D., Higashiyama, H., Bennett, V., Wu, P., & Huang, Z. J. (2004) · Cell · doi:10.1016/j.cell.2004.10.004 · Cited by 371 · see citing works
Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization
Nakada, C., Ritchie, K. P., Oba, Y., Nakamura, M., Hotta, Y., Iino, R., Kasai, R. S., Yamaguchi, K., Fujiwara, T. K., & Kusumi, A. (2003) · Nature Cell Biology · doi:10.1038/ncb1009 · Cited by 360 · see citing works
Perineuronal nets provide a polyanionic, glia‐associated form of microenvironment around certain neurons in many parts of the rat brain
Brückner, G., Brauer, K., Härtig, W., Wolff, J. R., Rickmann, M. J., Derouiche, A., Delpech, B., Girard, N., Oertel, W. H., & Reichenbach, A. (1993) · Glia · doi:10.1002/glia.440080306 · Cited by 358 · see citing works
Aggrecan Glycoforms Contribute to the Molecular Heterogeneity of Perineuronal Nets
Matthews, R. T., Kelly, G. M., Zerillo, C. A., Gray, G., Tiemeyer, M., & Hockfield, S. (2002) · The Journal of Neuroscience · doi:10.1523/jneurosci.22-17-07536.2002 · Cited by 341 · see citing works
Cortical neurons immunoreactive for the potassium channel Kv3.1b subunit are predominantly surrounded by perineuronal nets presumed as a buffering system for cations
Härtig, W., Derouiche, A., Welt, K., Brauer, K., Grosche, J., Mäder, M., Reichenbach, A., & Brückner, G. (1999) · Brain Research · doi:10.1016/s0006-8993(99)01784-9 · Cited by 339 · see citing works
The extracellular matrix of the central and peripheral nervous systems: structure and function
Rutka, J. T., Apodaca, G., Stern, R., & Rosenblum, M. L. (1988) · Journal of neurosurgery · doi:10.3171/jns.1988.69.2.0155 · Cited by 307 · see citing works
The C-type lectin domains of lecticans, a family of aggregating chondroitin sulfate proteoglycans, bind tenascin-R by protein– protein interactions independent of carbohydrate moiety
Aspberg, A., Miura, R., Bourdoulous, S., Shimonaka, M., Heinegård, D., Schachner, M., Ruoslahti, E., & Yamaguchi, Y. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.19.10116 · Cited by 275 · see citing works
Brevican-Deficient Mice Display Impaired Hippocampal CA1 Long-Term Potentiation but Show No Obvious Deficits in Learning and Memory
Brakebusch, C., Seidenbecher, C. I., Asztely, F., Rauch, U., Matthies, H., Meyer, H., Krug, M., Böckers, T. M., Zhou, X., Kreutz, M. R., et al. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.21.7417-7427.2002 · Cited by 264 · see citing works
Self-citation
Proline-Rich Synapse-Associated Protein-1/Cortactin Binding Protein 1 (ProSAP1/CortBP1) Is a PDZ-Domain Protein Highly Enriched in the Postsynaptic Density
Boeckers, T. M., Kreutz, M. R., Winter, C., Zuschratter, W., Smalla, K.-H., Sanmarti-Vila, L., Wex, H., Langnaese, K., Bockmann, J., Garner, C. C., et al. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-15-06506.1999 · Cited by 257 · see citing works
Self-citation
Interaction of voltage-gated sodium channels with the extracellular matrix molecules tenascin-C and tenascin-R
Srinivasan, J., Schachner, M., & Catterall, W. A. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15753 · Cited by 249 · see citing works
Perineuronal nets potentially protect against oxidative stress
Morawski, M., Brückner, M. K., Riederer, P., Brückner, G., & Arendt, T. (2004) · Experimental Neurology · doi:10.1016/j.expneurol.2004.04.017 · Cited by 246 · see citing works
Proteomics Analysis of Rat Brain Postsynaptic Density
Li, K. W., Hornshaw, M. P., van der Schors, R. C., Watson, R. B., Tate, S. A., Casetta, B., Jiménez, C. R., Gouwenberg, Y., Gundelfinger, E. D., Smalla, K., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m303116200 · Cited by 246 · see citing works
Self-citation
The Brain Chondroitin Sulfate Proteoglycan Brevican Associates with Astrocytes Ensheathing Cerebellar Glomeruli and Inhibits Neurite Outgrowth from Granule Neurons
Yamada, H., Fredette, B. J., Shitara, K., Hagihara, K., Miura, R., Ranscht, B., Stallcup, W. B., & Yamaguchi, Y. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-20-07784.1997 · Cited by 228 · see citing works
Brain-enriched Hyaluronan Binding (BEHAB)/Brevican Cleavage in a Glioma Cell Line Is Mediated by a Disintegrin and Metalloproteinase with Thrombospondin Motifs (ADAMTS) Family Member
Matthews, R. T., Gary, S. C., Zerillo, C. A., Pratta, M. A., Solomon, K. A., Arner, E. C., & Hockfield, S. J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m909764199 · Cited by 197 · see citing works
Brevican, a Chondroitin Sulfate Proteoglycan of Rat Brain, Occurs as Secreted and Cell Surface Glycosylphosphatidylinositol-anchored Isoforms
Seidenbecher, C. I., Richter, K., Rauch, U., Fässler, R., Garner, C. C., & Gundelfinger, E. D. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.45.27206 · Cited by 149 · see citing works
Self-citation
Neurocan: a brain chondroitin sulfate proteoglycan
Rauch, U., Feng, K., & Zhou, X.-H. (2001) · Cellular and Molecular Life Sciences · doi:10.1007/pl00000822 · Cited by 136 · see citing works
Proteomic analysis of synaptosomes using isotope‐coded affinity tags and mass spectrometry
Schrimpf, S., Meskenaite, V., Brunner, E., Rutishauser, D., Walther, P., Eng, J., Aebersold, R., & Sonderegger, P. (2005) · Proteomics · doi:10.1002/pmic.200401198 · Cited by 122 · see citing works
The Proteoglycan Lectin Domain Binds Sulfated Cell Surface Glycolipids and Promotes Cell Adhesion
Miura, R., Aspberg, A., Ethell, I. M., Hagihara, K., Schnaar, R. L., Ruoslahti, E. I., & Yamaguchi, Y. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.16.11431 · Cited by 119 · see citing works
Molecular cloning of Bral2, a novel brain-specific link protein, and immunohistochemical colocalization with brevican in perineuronal nets☆
Bekku, Y., Su, W.-D., Hirakawa, S., Fässler, R., Ohtsuka, A., Kang, J. S., Sanders, J., Murakami, T., Ninomiya, Y., & Oohashi, T. (2003) · Molecular and Cellular Neuroscience · doi:10.1016/s1044-7431(03)00133-7 · Cited by 114 · see citing works
Functional regions of the presynaptic cytomatrix protein bassoon: significance for synaptic targeting and cytomatrix anchoring
Dresbach, T., Hempelmann, A., Spilker, C., Dieck, S. T., Altrock, W. D., Zuschratter, W., Garner, C. C., & Gundelfinger, E. D. (2003) · Molecular and Cellular Neuroscience · doi:10.1016/s1044-7431(03)00015-0 · Cited by 113 · see citing works
Self-citation
Diffuse perineuronal nets and modified pyramidal cells immunoreactive for glutamate and the GABAA receptor α1 subunit form a unique entity in rat cerebral cortex
Wegner, F., Härtig, W., Bringmann, A., Grosche, J., Wohlfarth, K., Zuschratter, W., & Brückner, G. (2003) · Experimental Neurology · doi:10.1016/s0014-4886(03)00313-3 · Cited by 107 · see citing works
Neurons Produce a Neuronal Cell Surface-Associated Chondroitin Sulfate Proteoglycan
Lander, C., Zhang, H., & Hockfield, S. (1998) · The Journal of Neuroscience · doi:10.1523/jneurosci.18-01-00174.1998 · Cited by 107 · see citing works
[6] Aggrecan-versican-neurocan family of proteoglycans
Margolis, R. U., & Margolis, R. K. (1994) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(94)45008-0 · Cited by 93 · see citing works
Extracellular matrix alterations in brains lacking four of its components
Rauch, U., Zhou, X.-H., & Roos, G. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.01.026 · Cited by 88 · see citing works
Transcripts for secreted and GPI‐anchored brevican are differentially distributed in rat brain
Seidenbecher, C. I., Gundelfinger, E. D., Böckers, T. M., Trotter, J., & Kreutz, M. R. (1998) · European Journal of Neuroscience · doi:10.1046/j.1460-9568.1998.00166.x · Cited by 77 · see citing works
Self-citation
Proteomic comparison of two fractions derived from the transsynaptic scaffold
Phillips, G. R., Florens, L. A., Tanaka, H., Khaing, Z. Z., Fidler, L., Yates, J. R., & Colman, D. (2005) · Journal of Neuroscience Research · doi:10.1002/jnr.20614 · Cited by 74 · see citing works
Modeling an extracellular environment for axonal pathfinding and fasciculation in the central nervous system
Rauch, U. (1997) · Cell and Tissue Research · doi:10.1007/s004410050940 · Cited by 69 · see citing works
Protein components of a rat brain synaptic junctional protein preparation
Langnaese, K., Seidenbecher, C. I., Wex, H., Seidel, B., Hartung, K., APPELTAUER, U., Garner, A. M., Voss, B., Mueller, B., Garner, C. C., et al. (1996) · Molecular Brain Research · doi:10.1016/s0169-328x(96)00147-7 · Cited by 66 · see citing works
Self-citation
Brevican in the developing hippocampal fimbria: Differential expression in myelinating oligodendrocytes and adult astrocytes suggests a dual role for brevican in central nervous system fiber tract development
Ogawa, T., Hagihara, K., Suzuki, M., & Yamaguchi, Y. (2001) · The Journal of Comparative Neurology · doi:10.1002/cne.1103 · Cited by 61 · see citing works
Synaptogenesis in hippocampal cultures
Verderio, C., Coco, S., Pravettoni, E., Bacci, A., & Matteoli, M. (1999) · Cellular and Molecular Life Sciences · doi:10.1007/s000180050384 · Cited by 59 · see citing works
Brevican isoforms associate with neural membranes
Seidenbecher, C. I., Smalla, K., Fischer, N., Gundelfinger, E. D., & Kreutz, M. R. (2002) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2002.01183.x · Cited by 55 · see citing works
Self-citation
Altered production and proteolytic processing of brevican by transforming growth factor β in cultured astrocytes
Hamel, M. G., Mayer, J., & Gottschall, P. E. (2005) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2005.03144.x · Cited by 55 · see citing works
Perisynaptic barrier of proteoglycans in the mature brain and spinal cord
MURAKAMI, T., & Ohtsuka, A. (2003) · Archivum histologicum japonicum · doi:10.1679/aohc.66.195 · Cited by 53 · see citing works
BEHAB/brevican: a brain-specific lectican implicated in gliomas and glial cell motility
Gary, S. C., Kelly, G. M., & Hockfield, S. J. (1998) · Current Opinion in Neurobiology · doi:10.1016/s0959-4388(98)80083-4 · Cited by 50 · see citing works
Perineuronal nets show intrinsic patterns of extracellular matrix differentiation in organotypic slice cultures
Grosche, J., & Br�ckner, G. (2001) · Experimental Brain Research · doi:10.1007/s002210000617 · Cited by 50 · see citing works
Glial Tumor Invasion: A Role for the Upregulation and Cleavage of BEHAB/Brevican
Nutt, C. L., Matthews, R. T., & Hockfield, S. J. (2001) · The Neuroscientist · doi:10.1177/107385840100700206 · Cited by 49 · see citing works
Confocal microscopic estimation of GABAergic nerve terminals in the central nervous system
Hiscock, J. J., Murphy, S., & Willoughby, J. O. (2000) · Journal of Neuroscience Methods · doi:10.1016/s0165-0270(99)00163-6 · Cited by 48 · see citing works
Brain enriched hyaluronan binding (BEHAB)/brevican increases aggressiveness of CNS-1 gliomas in Lewis rats.
Nutt, C. L., Zerillo, C. A., Kelly, G. M., & Hockfield, S. J. (2001) · PubMed · Cited by 46 · see citing works
Carbohydrate–protein interactions between HNK‐1‐reactive sulfoglucuronyl glycolipids and the proteoglycan lectin domain mediate neuronal cell adhesion and neurite outgrowth
Miura, R., Ethell, I. M., & Yamaguchi, Y. (2001) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2001.00042.x · Cited by 39 · see citing works
Sequence and Chromosomal Localization of the Mouse Brevican Gene
Rauch, U., Meyer, H., Brakebusch, C., Seidenbecher, C. I., Gundelfinger, E. D., Beier, D. R., & Fässler, R. (1997) · Genomics · doi:10.1006/geno.1997.4853 · Cited by 25 · see citing works
Self-citation
Postnatal development of perineuronal nets in wild-type mice and in a mutant deficient in tenascin-R
Br�ckner, G., Grosche, J., Schmidt, S., H�rtig, W., Margolis, R. U., Delpech, B., Seidenbecher, C. I., Czaniera, R., & Schachner, M. (2000) · The Journal of Comparative Neurology · doi:10.1002/1096-9861(20001225)428:4
Self-citation
Untitled work
Hagihara (1999) · doi:10.1002/(sici)1096-9861(19990726)410:2
Autoradiographic and in situ hybridization localization of corticotropin-releasing factor 1 and 2 receptors in nonhuman primate brain
S�nchez, M. M., Young, L. J., Plotsky, P. M., & Insel, T. R. (1999) · The Journal of Comparative Neurology · doi:10.1002/(sici)1096-9861(19990607)408:3
Self-citation

Flagged first, then by citations

1 of the 55 references couldn’t be matched to a known paper.

Sorted by citations

The roles of perineuronal nets and the perinodal extracellular matrix in neuronal function
Fawcett, J. W., Oohashi, T., & Pizzorusso, T. (2019) · Nature Reviews Neuroscience · doi:10.1038/s41583-019-0196-3 · Cited by 589 · see citing works
Brain extracellular matrix affects AMPA receptor lateral mobility and short-term synaptic plasticity
Frischknecht, R., Heine, M., Perrais, D., Seidenbecher, C. I., Choquet, D., & Gundelfinger, E. D. (2009) · Nature Neuroscience · doi:10.1038/nn.2338 · Cited by 554 · see citing works
Self-citation
Extracellular matrix of the central nervous system: from neglect to challenge
Zimmermann, D. R., & Dours-Zimmermann, M. T. (2008) · Histochemistry and Cell Biology · doi:10.1007/s00418-008-0485-9 · Cited by 465 · see citing works
“are in vitro primarily astrocyte-derived (John et al. 2006), although perineuronal net-like structures develop apparently also in the virtual absence of glial cells (Miyata et al.”
Background
The axon initial segment and the maintenance of neuronal polarity
Rasband, M. N. (2010) · Nature Reviews Neuroscience · doi:10.1038/nrn2852 · Cited by 442 · see citing works
“Thus, the unique extracellular matrix found at the AI”
Background
The perineuronal net and the control of CNS plasticity
Wang, D., & Fawcett, J. (2012) · Cell and Tissue Research · doi:10.1007/s00441-012-1375-y · Cited by 373 · see citing works
Activity‐dependent formation and functions of chondroitin sulfate‐rich extracellular matrix of perineuronal nets
Dityatev, A., Brückner, G., Dityateva, G., Grosche, J., Kleene, R., & Schachner, M. (2007) · Developmental Neurobiology · doi:10.1002/dneu.20361 · Cited by 365 · see citing works
“Another recent study revealed that both excitatory and inhibitory synapses in dissociated hippocampal cultures are embedded in the extracellular matrix of PNs (John et al., 2006).”
BackgroundInfluential
Neurofascin assembles a specialized extracellular matrix at the axon initial segment
Hedstrom, K. L., Xu, X., Ogawa, Y., Frischknecht, R., Seidenbecher, C. I., Shrager, P., & Rasband, M. N. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200705119 · Cited by 268 · see citing works
“The guinea pig anti-brevican antibodies were previously described (John et al., 2006).”
BackgroundResultInfluentialSelf-citation
Crosstalk between glia, extracellular matrix and neurons
Song, I., & Dityatev, A. E. (2017) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2017.03.003 · Cited by 263 · see citing works
Compartmentalization from the outside: the extracellular matrix and functional microdomains in the brain
Dityatev, A. E., Seidenbecher, C. I., & Schachner, M. (2010) · Trends in Neurosciences · doi:10.1016/j.tins.2010.08.003 · Cited by 229 · see citing works
Self-citation
Perineuronal net formation and structure in aggrecan knockout mice
Giamanco, K. A., Morawski, M., & Matthews, R. T. (2010) · Neuroscience · doi:10.1016/j.neuroscience.2010.08.032 · Cited by 207 · see citing works
Contributions of astrocytes to synapse formation and maturation — Potential functions of the perisynaptic extracellular matrix
Faissner, A., Pyka, M., Geissler, M., Sobik, T., Frischknecht, R., Gundelfinger, E. D., & Seidenbecher, C. (2010) · Brain Research Reviews · doi:10.1016/j.brainresrev.2010.01.001 · Cited by 207 · see citing works
Self-citation
Tissue-specific extracellular matrix accelerates the formation of neural networks and communities in a neuron-glia co-culture on a multi-electrode array
Lam, D., Enright, H. A., Cadena, J., Peters, S. K. G., Sales, A. P., Osburn, J. J., Soscia, D. A., Kulp, K. S., Wheeler, E. K., & Fischer, N. O. (2019) · Scientific Reports · doi:10.1038/s41598-019-40128-1 · Cited by 205 · see citing works
Axon initial segments: structure, function, and disease
Huang, C. Y., & Rasband, M. N. (2018) · Annals of the New York Academy of Sciences · doi:10.1111/nyas.13718 · Cited by 187 · see citing works
Chondroitin sulfate proteoglycans: Key modulators in the developing and pathologic central nervous system
Dyck, S. M., & Karimi‐Abdolrezaee, S. (2015) · Experimental Neurology · doi:10.1016/j.expneurol.2015.04.006 · Cited by 189 · see citing works
Extracellular matrix abnormalities in schizophrenia
Berretta, S. (2012) · Neuropharmacology · doi:10.1016/j.neuropharm.2011.08.010 · Cited by 179 · see citing works
<i>In vitro</i> modeling of perineuronal nets: hyaluronan synthase and link protein are necessary for their formation and integrity
Kwok, J. C. F., Carulli, D., & Fawcett, J. W. (2010) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2010.06878.x · Cited by 170 · see citing works
“Neurons have some ability to make PNNs in long-term culture (Miyata et al. 2005; John et al. 2006).”
Background
Formation and remodeling of the brain extracellular matrix in neural plasticity: Roles of chondroitin sulfate and hyaluronan
Miyata, S., & Kitagawa, H. (2017) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2017.06.010 · Cited by 171 · see citing works
“Although several PNN components such as brevican and tenascin-R are synthesized by astrocytes [175,176], the contribution of astrocytes to PNN formation is less clear.”
Background
Protein kinase CK2 contributes to the organization of sodium channels in axonal membranes by regulating their interactions with ankyrin G
Bréchet, A., Fache, M., Brachet, A., Ferracci, G., Baude, A., Irondelle, M., Pereira, S., Leterrier, C., & Dargent, B. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200805169 · Cited by 172 · see citing works
The Brain’s Extracellular Matrix and Its Role in Synaptic Plasticity
Frischknecht, R., & Gundelfinger, E. D. (2012) · Advances in Experimental Medicine and Biology · doi:10.1007/978-3-7091-0932-8_7 · Cited by 156 · see citing works
Self-citation
Upregulation of aggrecan, link protein 1, and hyaluronan synthases during formation of perineuronal nets in the rat cerebellum
Carulli, D., Rhodes, K. E., & Fawcett, J. W. (2007) · Journal of Comparative Neurology · doi:10.1002/cne.21231 · Cited by 158 · see citing works
“However, a recent study showed that brevican surrounding hippocampal neurons in vitro is produced by cocultured astrocytes, indicating that neurons have the capability to attract brevican synthesized from other cells into their nets (John et al., 2006).”
Background
Losing the sugar coating: Potential impact of perineuronal net abnormalities on interneurons in schizophrenia
Berretta, S., Pantazopoulos, H., Markota, M., Brown, C., & Batzianouli, E. T. (2015) · Schizophrenia Research · doi:10.1016/j.schres.2014.12.040 · Cited by 152 · see citing works
“contribute to the formation andmaintenance of PNNs (John et al., 2006; Faissner et al., 2010; Klausmeyer et al., 2011; Wiese et al., 2012).”
Primary Hippocampal Neurons, Which Lack Four Crucial Extracellular Matrix Molecules, Display Abnormalities of Synaptic Structure and Function and Severe Deficits in Perineuronal Net Formation
Geissler, M., Gottschling, C., Aguado, A., Rauch, U., Wetzel, C. H., Hatt, H., & Faissner, A. (2013) · The Journal of Neuroscience · doi:10.1523/jneurosci.3275-12.2013 · Cited by 140 · see citing works
“1) confirms earlier reports that highlighted the localization at the axon hillock (John et al., 2006; Hedstrom et al., 2007).”
BackgroundResult
Deconstructing the perineuronal net: Cellular contributions and molecular composition of the neuronal extracellular matrix
Giamanco, K. A., & Matthews, R. T. (2012) · Neuroscience · doi:10.1016/j.neuroscience.2012.05.055 · Cited by 138 · see citing works
“Given the complexity of the extracellular environment in vivo, many laboratories have opted to utilize culture models to study PNN formation and development (Brückner and Grosche, 2001; Miyata et al., 2005; John et al., 2006; Dityatev et al., 2007; Reimers et al., 2007).”
BackgroundInfluential
Converting juvenile into adult plasticity: a role for the brain's extracellular matrix
Gundelfinger, E. D., Frischknecht, R., Choquet, D., & Heine, M. (2010) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2010.07253.x · Cited by 136 · see citing works
“CNS areas after prolonged time in culture (Miyata et al., 2005; John et al., 2006; Dityatev et al., 2007).”
BackgroundInfluentialSelf-citation
Chondroitin sulfate proteoglycans regulate astrocyte‐dependent synaptogenesis and modulate synaptic activity in primary embryonic hippocampal neurons
Pyka, M., Wetzel, C., Aguado, A., Geissler, M., Hatt, H., & Faissner, A. (2011) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2011.07690.x · Cited by 131 · see citing works
“Expression of ECM glycoproteins and emergence of perineural net structures in the cell-insert co-culture system Recent studies have reported on the ECM of PNNs formed in dissociated hippocampal primary cultures (John et al., 2006; Frischknecht et al., 2009).”
BackgroundResultInfluential
The hyaluronan and proteoglycan link proteins: Organizers of the brain extracellular matrix and key molecules for neuronal function and plasticity
Oohashi, T., Edamatsu, M., Bekku, Y., & Carulli, D. (2015) · Experimental Neurology · doi:10.1016/j.expneurol.2015.09.010 · Cited by 131 · see citing works
In Sickness and in Health: Perineuronal Nets and Synaptic Plasticity in Psychiatric Disorders
Pantazopoulos, H., & Berretta, S. (2015) · Neural Plasticity · doi:10.1155/2016/9847696 · Cited by 118 · see citing works
The functional organization and assembly of the axon initial segment
Ogawa, Y., & Rasband, M. N. (2008) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2008.08.008 · Cited by 114 · see citing works
Ankyrin-Dependent and -Independent Mechanisms Orchestrate Axonal Compartmentalization of L1 Family Members Neurofascin and L1/Neuron–Glia Cell Adhesion Molecule
Boiko, T., Vakulenko, M., Ewers, H., Yap, C. C., Norden, C., & Winckler, B. (2007) · Journal of Neuroscience · doi:10.1523/jneurosci.4302-06.2007 · Cited by 112 · see citing works
“Importantly, multiple complex extracellular interactions take place at the IS in vivo (Ango et al., 2004; Eshed et al., 2005; Bruckner et al., 2006; John et al., 2006).”
Background
Brevican: A key proteoglycan in the perisynaptic extracellular matrix of the brain
Frischknecht, R., & Seidenbecher, C. I. (2012) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2012.03.022 · Cited by 112 · see citing works
Self-citation
Tenascin-R promotes assembly of the extracellular matrix of perineuronal nets via clustering of aggrecan
Morawski, M., Dityatev, A., Hartlage-Rübsamen, M., Blosa, M., Holzer, M., Flach, K., Pavlica, S., Dityateva, G., Grosche, J., Brückner, G., et al. (2014) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2014.0046 · Cited by 100 · see citing works
“The molecular composition of PNs in vivo and in vitro appears to be similar in regard to ECM components, such as aggrecan [6,7], brevican [3,8] and the binding sites for the lectin Wisteria floribunda agglutinin (WFA) [2,5].”
Background
Building and maintaining the axon initial segment
Grubb, M. S., & Burrone, J. (2010) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2010.04.012 · Cited by 106 · see citing works
Brevican distinctively assembles extracellular components at the large diameter nodes of Ranvier in the CNS
Bekku, Y., Rauch, U., Ninomiya, Y., & Oohashi, T. (2009) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2009.05873.x · Cited by 104 · see citing works
“More recently, it was also recognized in both the axonal nodes and the related initial axonal segments, where it accumulated in a neurofascin 186 dependent manner (John et al. 2006; Hedstrom et al. 2007).”
Background
Keratan Sulfate Restricts Neural Plasticity after Spinal Cord Injury
Imagama, S., Sakamoto, K., Tauchi, R., Shinjo, R., Ohgomori, T., Ito, Z., Zhang, H. M., Nishida, Y., Asami, N., Takeshita, S., et al. (2011) · Journal of Neuroscience · doi:10.1523/jneurosci.5120-10.2011 · Cited by 98 · see citing works
“Perineuronal nets are prominent on GABAergic interneurons, but can be detected on virtually all neurons (Celio and Blümcke, 1994; Celio et al., 1998; John et al., 2006).”
Background
Brain extracellular matrix retains connectivity in neuronal networks
Bikbaev, A., Frischknecht, R., & Heine, M. (2015) · Scientific Reports · doi:10.1038/srep14527 · Cited by 88 · see citing works
Self-citation
Organization and maintenance of molecular domains in myelinated axons
Buttermore, E. D., Thaxton, C., & Bhat, M. A. (2013) · Journal of Neuroscience Research · doi:10.1002/jnr.23197 · Cited by 92 · see citing works
Increased Excitability and Reduced Excitatory Synaptic Input Into Fast-Spiking CA2 Interneurons After Enzymatic Attenuation of Extracellular Matrix
Hayani, H., Song, I., & Dityatev, A. (2018) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2018.00149 · Cited by 84 · see citing works
The crosstalk of hyaluronan-based extracellular matrix and synapses
Frischknecht, R., & Seidenbecher, C. I. (2008) · Neuron Glia Biology · doi:10.1017/s1740925x09990226 · Cited by 84 · see citing works
Self-citation
The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure
Eill, G. J., Sinha, A., Morawski, M., Viapiano, M. S., & Matthews, R. T. (2020) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)49907-8 · Cited by 32 · see citing works
Microglia Depletion-Induced Remodeling of Extracellular Matrix and Excitatory Synapses in the Hippocampus of Adult Mice
Strackeljan, L., Baczynska, E., Cangalaya, C., Baidoe-Ansah, D., Wlodarczyk, J., Kaushik, R., & Dityatev, A. (2021) · Cells · doi:10.3390/cells10081862 · Cited by 71 · see citing works
“The following primary reagents were used: chicken anti-PV (1:500, Synaptic Systems, Göttingen, Germany, 195006), biotinylated Wisteria floribunda agglutinin (WFA, 1:1000, Vector laboratories B-1355, Burlingame, CA, USA), rabbit anti-Bcan [32,33] (1:1000), guinea pig anti-vGluT1 (1:1500, Synaptic Systems, 135304), mous…”
Method
The Axon Initial Segment, 50 Years Later
Leterrier, C. (2015) · Current topics in membranes · doi:10.1016/bs.ctm.2015.10.005 · Cited by 79 · see citing works
Diverse functions of perineuronal nets
Karetko, M., & Skangiel-Kramska, J. (2009) · Acta Neurobiologiae Experimentalis · doi:10.55782/ane-2009-1766 · Cited by 76 · see citing works
“These results indicate that tenascin-R is essential for ECM proteoglycans to form the lattice-like structure (Weber et al. 1999, Haunso et al. 2000).”
BackgroundInfluential
Expression and role of the cell surface protease seprase/fibroblast activation protein-α (FAP-α) in astroglial tumors
Mentlein, R., Hattermann, K., Hemion, C., Jungbluth, A. A., & Held‐Feindt, J. (2010) · Biological Chemistry · doi:10.1515/bc.2010.119 · Cited by 76 · see citing works
“Brevican exists in two splice variants, the dominant secreted form with a 97 kDa core protein and approximately 100 kDa of attached chondroitin sulfate, and a glycosylphosphatidylinositol-anchored, truncated form; both are primarily synthesized by astroglial cells (Gary et al., 2000; John et al., 2006).”
Background
Matrix-Dependent Local Retention of Secretory Vesicle Cargo in Cortical Neurons
de Wit, J., Toonen, R. F. G., & Verhage, M. (2009) · Journal of Neuroscience · doi:10.1523/jneurosci.3931-08.2009 · Cited by 73 · see citing works
“In these cases release was strictly synaptic and the extracellular presence may therefore be attributable to interactions with the ECM, which is concentrated at synapses (John et al., 2006).”
Background
Astrocytes in Neural Circuits: Key Factors in Synaptic Regulation and Potential Targets for Neurodevelopmental Disorders
Liu, X., Ying, J., Wang, X., Zheng, Q., Zhao, T., Yoon, S., Yu, W., Yang, D., Yang, F., & Hua, F. (2021) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2021.729273 · Cited by 70 · see citing works
Pinceau Organization in the Cerebellum Requires Distinct Functions of Neurofascin in Purkinje and Basket Neurons during Postnatal Development
Buttermore, E. D., Piochon, C., Wallace, M. L., Philpot, B. D., Hansel, C., & Bhat, M. A. (2012) · Journal of Neuroscience · doi:10.1523/jneurosci.5602-11.2012 · Cited by 68 · see citing works
“, 2010), and rabbit anti-Brevican (John et al., 2006).”
Background
Intrinsic and extrinsic determinants of ion channel localization in neurons
Hedstrom, K. L., & Rasband, M. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.04001.x · Cited by 67 · see citing works
“Finally, two recent reports showed that there is an enrichment of the extracellular matrix (ECM) molecules aggrecan and brevican around the AIS (Bruckner et al. 2006; John et al. 2006).”
Background
Multimodal signaling by the ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs) promotes neurite extension
Hamel, M. G., Ajmo, J. M., Leonardo, C. C., Zuo, F., Sandy, J. D., & Gottschall, P. E. (2007) · Experimental Neurology · doi:10.1016/j.expneurol.2007.11.014 · Cited by 60 · see citing works
“The glial scar is an accumulation of reactive astrocytes and extracellular matrix (ECM) molecules such as chondroitin sulfate (CS)-substituted PGs, tenascin and other proteins that inhibit the re-growth of axons and the migration of certain cells into the damaged region (Davies, et al., 1999, Davies, et al., 1997, Lay…”
MethodInfluential
Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body – Implications for physiological functions
Blosa, M., Sonntag, M., Brückner, G., Jäger, C., Seeger, G., Matthews, R. T., Rübsamen, R., Arendt, T., & Morawski, M. (2012) · Neuroscience · doi:10.1016/j.neuroscience.2012.10.003 · Cited by 59 · see citing works
Composition, assembly, and maintenance of excitable membrane domains in myelinated axons
Rasband, M. N. (2010) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2010.09.010 · Cited by 59 · see citing works
“The chondroitin sulfate proteoglycans brevican and versican are both found at the AIS and CNS nodes of Ranvier [47-49].”
Background
Mimicking the Brain Extracellular Matrix in Vitro: A Review of Current Methodologies and Challenges
Rauti, R., Renous, N., & Maoz, B. M. (2019) · Israel Journal of Chemistry · doi:10.1002/ijch.201900052 · Cited by 57 · see citing works
Expression of chondroitin sulfate proteoglycans in barrel field of mouse and rat somatosensory cortex
Nakamura, M., Nakano, K., Morita, S., Nakashima, T., Oohira, A., & Miyata, S. (2009) · Brain Research · doi:10.1016/j.brainres.2008.11.022 · Cited by 54 · see citing works
“CSPGs consist of a core protein and sulfated glycosaminoglycans (GAGs) (Bandtlow and Zimmermann, 2000; Yamaguchi, 2000) and are generally known to inhibit neurite outgrowth (Oohira et al., 1991; Katoh-Senba et al., 1995).”
Neural ECM molecules in axonal and synaptic homeostatic plasticity
Frischknecht, R., Chang, K.-J., Rasband, M. N., & Seidenbecher, C. I. (2014) · Progress in brain research · doi:10.1016/b978-0-444-63486-3.00004-9 · Cited by 53 · see citing works
Self-citation
Matrix Metalloproteinase-19 is Highly Expressed in Astroglial Tumors and Promotes Invasion of Glioma Cells
Lettau, I. D., Hattermann, K., Held‐Feindt, J., Brauer, R., Sedlacek, R., & Mentlein, R. (2010) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/nen.0b013e3181ce9f67 · Cited by 52 · see citing works
“Brevican exists in 2 splice variants, the dominant secreted form with a 97-kd core protein and approximately 60 kd of attached chondroitin sulfate, and a glycosylphosphatidylinositol-anchored truncated form; both are primarily synthesized by astroglial cells (41).”
Background
Hyaluronan-based extracellular matrix under conditions of homeostatic plasticity
Valenzuela, J. C., Heise, C., Franken, G., Singh, J. B., Schweitzer, B., Seidenbecher, C. I., & Frischknecht, R. (2014) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2013.0606 · Cited by 46 · see citing works
Self-citation
Hyaluronan synthesis by developing cortical neurons in vitro
Fowke, T. M., Karunasinghe, R. N., Bai, J.-Z., Jordan, S., Gunn, A. J., & Dean, J. M. (2017) · Scientific Reports · doi:10.1038/srep44135 · Cited by 47 · see citing works
“In the present study, we found no obvious overlap of hyaluronan with synaptophysin or PSD-95, which is supported by evidence that perineuronal net components enclose synapses, but are absent from the sites of synaptic contact 46,58 .”
Influential
Dopamine Receptor Activation Modulates the Integrity of the Perisynaptic Extracellular Matrix at Excitatory Synapses
Mitlöhner, J., Kaushik, R., Niekisch, H., Blondiaux, A., Gee, C. E., Happel, M. F. K., Gundelfinger, E., Dityatev, A., Frischknecht, R., & Seidenbecher, C. (2020) · Cells · doi:10.3390/cells9020260 · Cited by 47 · see citing works
“As previously reported by us and others, at this time-point, the cultures had reliably developed a HAbased ECM with similar composition as in vivo [17,18,20].”
Self-citation
The Synaptic Extracellular Matrix: Long-Lived, Stable, and Still Remarkably Dynamic
Dankovich, T. M., & Rizzoli, S. O. (2022) · Frontiers in Synaptic Neuroscience · doi:10.3389/fnsyn.2022.854956 · Cited by 44 · see citing works
Excitable Domains of Myelinated Nerves
Chang, K.-J., & Rasband, M. N. (2013) · Current topics in membranes · doi:10.1016/b978-0-12-417027-8.00005-2 · Cited by 42 · see citing works
Extracellular matrixes and neuroinflammation
Jang, D. G., Sim, H. J., Song, E. K., Kwon, T., & Park, T. J. (2020) · BMB Reports · doi:10.5483/bmbrep.2020.53.10.156 · Cited by 42 · see citing works
“It is expressed by neurons, astrocytes, and oligodendrocytes (25-27).”
Background
Neural ECM and synaptogenesis
Heikkinen, A., Pihlajaniemi, T., Faissner, A., & Yuzaki, M. (2014) · Progress in Brain Research · doi:10.1016/b978-0-444-63486-3.00002-5 · Cited by 42 · see citing works
Abnormal post‐translational and extracellular processing of brevican in plaque‐bearing mice over‐expressing APPsw
Ajmo, J. M., Bailey, L. A., Howell, M. D., Cortez, L. K., Pennypacker, K. R., Mehta, H. N., Morgan, D., Gordon, M. N., & Gottschall, P. E. (2010) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2010.06647.x · Cited by 41 · see citing works
“Brevican-containing, dense network of ECM in the nervous system, wraps around synapses where irregularly deposited proteins bind to anchored membrane ‘receptors’ to form surface compartments around the synapse (John et al. 2006; Frischknecht et al. 2009).”
Enzymatic Degradation of Cortical Perineuronal Nets Reverses GABAergic Interneuron Maturation
Willis, A., Pratt, J. A., & MORRIS, B. J. (2022) · Molecular Neurobiology · doi:10.1007/s12035-022-02772-z · Cited by 35 · see citing works
“1c), a CSPG which is prevalent throughout the mature PNN structure but is thought to be enriched around the axonal initial segment [64, 65], after ChABC treatment.”
Background
Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex
Virgintino, D., Perissinotto, D., Girolamo, F., Mucignat, M. T., Montanini, L., Errede, M., Kaneiwa, T., Yamada, S., Sugahara, K., Roncali, L., et al. (2009) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2009.00694.x · Cited by 36 · see citing works
“All members of the lectican family have been shown to be synthetic products of cultured multipotent neural progenitor cells [12, 27], capable of reproducing PNN-like structures [28], and aggrecan seems to be specifically involved in neural cell–cell aggregation phenomena [29–30].”
Background
Total number, distribution, and phenotype of cells expressing chondroitin sulfate proteoglycans in the normal human amygdala
Pantazopoulos, H., Murray, E. A., & Berretta, S. (2008) · Brain Research · doi:10.1016/j.brainres.2008.02.036 · Cited by 34 · see citing works
Expression profiling of human glial precursors
Campanelli, J. T., Sandrock, R. W., Wheatley, W., Xue, H., Zheng, J., Liang, F., Chesnut, J. D., Zhan, M., Rao, M. S., & Liu, Y. M. (2008) · BMC Developmental Biology · doi:10.1186/1471-213x-8-102 · Cited by 34 · see citing works
“Brevican is a neural-specific chondroitin sulfate proteoglycan and is expressed in mature astrocytes and downregulated in adult rat spinal cords [50,51].”
Loss of Interneuron-Derived Collagen XIX Leads to a Reduction in Perineuronal Nets in the Mammalian Telencephalon
Su, J., Cole, J., & Fox, M. A. (2017) · ASN NEURO · doi:10.1177/1759091416689020 · Cited by 33 · see citing works
Presynaptic secretion of mind‐the‐gap organizes the synaptic extracellular matrix‐integrin interface and postsynaptic environments
Rushton, E., Rohrbough, J. C., & Broadie, K. S. (2009) · Developmental Dynamics · doi:10.1002/dvdy.21864 · Cited by 34 · see citing works
The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure
Eill, G. J., Sinha, A., Morawski, M., Viapiano, M. S., & Matthews, R. T. (2019) · Journal of Biological Chemistry · doi:10.1074/jbc.ra119.010830 · Cited by 32 · see citing works
Hyaluronan oligosaccharides promote functional recovery after spinal cord injury in rats
Wakao, N., Imagama, S., Zhang, H., Tauchi, R., Muramoto, A., Natori, T., Takeshita, S., Ishiguro, N., Matsuyama, Y., & Kadomatsu, K. (2010) · Neuroscience Letters · doi:10.1016/j.neulet.2010.11.051 · Cited by 31 · see citing works
The emerging role of the chondroitin sulfate proteoglycan family in neurodegenerative diseases
Lin, J., Duan, M.-R., Lin, N., & Zhao, W. (2021) · Reviews in the Neurosciences · doi:10.1515/revneuro-2020-0146 · Cited by 29 · see citing works
Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices
Ueno, H., Suemitsu, S., Murakami, S., Kitamura, N., Wani, K., Matsumoto, Y., Okamoto, M., & Ishihara, T. (2018) · IBRO Reports · doi:10.1016/j.ibror.2018.11.006 · Cited by 26 · see citing works
“Brevican has been shown to be significantly involved in nerve excitability in the axon initial segment (John et al., 2006).”
Background
Modulatory properties of extracellular matrix glycosaminoglycans and proteoglycans on neural stem cells behavior: Highlights on regenerative potential and bioactivity
Shabani, Z., Ghadiri, T., Karimipour, M., Sadigh‐Eteghad, S., Mahmoudi, J., Mehrad, H., & Farhoudi, M. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.01.006 · Cited by 28 · see citing works
Linking epileptic phenotypes and neural extracellular matrix remodeling signatures in mouse models of epilepsy
Blondiaux, A., Jia, S., Annamneedi, A., Çalışkan, G., Nebel, J., Montenegro‐Venegas, C., Wykes, R. C., Fejtová, A., Walker, M. C., Stork, O., et al. (2023) · Neurobiology of Disease · doi:10.1016/j.nbd.2023.106324 · Cited by 26 · see citing works
“…1:1000 for Western blots [WB] and 1:500 for immunohistochemistry [IHC]) and rabbit anti-Brevican (1:1000 for WB) (Seidenbecher et al., 1995; John et al., 2006); rabbit anti-Aggrecan (Millipore, AB1031, 1:500 for WB; RRID:AB_90460); sheep anti-Neurocan (R & D Systems, AF5800; 1:200 for WB;…”
Self-citation
Influence of glial‐derived matrix molecules, especially chondroitin sulfates, on neurite growth and survival of cultured mouse embryonic motoneurons
Klausmeyer, A., Conrad, R., Faissner, A., & Wiese, S. (2011) · Journal of Neuroscience Research · doi:10.1002/jnr.22531 · Cited by 24 · see citing works
“…isoforms of phosphacan/RPTPb (rabbit polyclonal 1:50; Klausmeyer et al., 2007); pAb NC-2 antineurocan (rabbit polyclonal 1:200; Retzler et al., 1996); pAb antibrevican (guinea pig polyclonal 1:200; John et al., 2006); and pAb KAF14 anti-tenascin-C (rabbit polyclonal 1:200; Faissner et al., 1990).”
Background
A Composite Sketch of Fast-Spiking Parvalbumin-Positive Neurons
Bartholomé, O., Bonardeaux, O. D. L. B., Neirinckx, V., & Rogister, B. (2020) · Cerebral Cortex Communications · doi:10.1093/texcom/tgaa026 · Cited by 21 · see citing works
“In cortical and hippocampal PV+ neurons, it has been shown that the majority of AMPARs lacks the GluA2 subunit, making it permeable to Ca2+ (Jonas et al. 1994; Geiger et al. 1995; Talos et al. 2006).”
Background
The brain as a “hyper-network”: the key role of neural networks as main producers of the integrated brain actions especially via the “broadcasted” neuroconnectomics
Agnati, L. F., Marcoli, M., Maura, G., Woods, A. S., & Guidolin, D. (2018) · Journal of Neural Transmission · doi:10.1007/s00702-018-1855-7 · Cited by 22 · see citing works
“These data have been the experimental background for introducing the new concept of ‘tetra-partite synapse’ for the complex formed by the classical synaptic contact of the glial processes (in particular the PAPs) surrounding them and, in addition, ECM peri-synaptic condensations (John et al. 2006; Dityatev et al. 2010…”
Diverse impact of acute and long-term extracellular proteolytic activity on plasticity of neuronal excitability
Wójtowicz, T., Brzdąk, P., & Mozrzymas, J. W. (2015) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2015.00313 · Cited by 20 · see citing works
“Of note, tenascins can bind to neuronal sodium channels and play an important role in regulation of sodium channel density at axon initial segments (AISs) and nodes of Ranvier (Srinivasan et al., 1998).”
BackgroundInfluential
Deficits in developmental neurogenesis and dendritic spine maturation in mice lacking the serine protease inhibitor neuroserpin
Hermann, M., Reumann, R., Schostak, K., Kement, D., Gelderblom, M., Bernreuther, C., Frischknecht, R., Schipanski, A., Marik, S., Krasemann, S., et al. (2019) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2019.103420 · Cited by 21 · see citing works
“2007), aggrecan (1:50, Millipore AB1031), brevican (1:50) (John et al. 2006), GFAP (1:500, Millipore MAB360), Iba1 (1:500, Wako 019-19741) or NeuN (1:50, Millipore MAB377) in blocking buffer containing 0,2 % Triton X-100.”
Self-citation
Membrane-Bound Catechol-O-Methyl Transferase in Cortical Neurons and Glial Cells is Intracellularly Oriented
Schott, B. H., Frischknecht, R., Dębska–Vielhaber, G., John, N., Behnisch, G., Düzel, E., Gundelfinger, E. D., & Seidenbecher, C. I. (2010) · Frontiers in Psychiatry · doi:10.3389/fpsyt.2010.00142 · Cited by 20 · see citing works
Self-citation
Hyaluronic acid based extracellular matrix regulates surface expression of GluN2B containing NMDA receptors
Schweitzer, B., Singh, J., Fejtova, A., Groc, L., Heine, M., & Frischknecht, R. (2017) · Scientific Reports · doi:10.1038/s41598-017-07003-3 · Cited by 19 · see citing works
Self-citation
Do Perineuronal Nets Stabilize the Engram of a Synaptic Circuit?
Lev‐Ram, V., Lemieux, S. P., Deerinck, T. J., Bushong, E. A., Perez, A. J., Pritchard, D. R., Toyama, B. H., Park, S. K. R., McClatchy, D. B., Savas, J. N., et al. (2024) · Cells · doi:10.3390/cells13191627 · Cited by 15 · see citing works
“…using light microscopy (LM) in cultured neurons [27] and in vivo [26], but this has never been confirmed by high-resolution 3D-EM. above observation has been reported using light microscopy (LM) in cultured neurons [27] and in vivo [26], but this has never been confirmed by high-resolution 3D-EM.”
BackgroundMethod
Spatial patterns and cell surface clusters in perineuronal nets
Arnst, N., Kuznetsova, S., Lipachev, N., Shaikhutdinov, N., Melnikova, A., Олегович, М. М., Uvarov, P., Baltina, T., Rauvala, H., Osin, Y. N., et al. (2016) · Brain Research · doi:10.1016/j.brainres.2016.07.020 · Cited by 15 · see citing works
“Accordingly, the diffusion rates of AMPA receptors in cultured hippocampal neurons are reduced between the 2 nd and 3 rd weeks in culture – at the time when the PNN-like structure is formed on the neuronal surface (Borgdorff and Choquet 2002; John et al., 2006).”
Neurocan regulates axon initial segment organization and neuronal activity
Baidoe‐Ansah, D., Mirzapourdelavar, H., Aleshin, S. E., Schott, B. H., Seidenbecher, C. I., Kaushik, R., & Dityatev, A. E. (2025) · Matrix Biology · doi:10.1016/j.matbio.2025.01.001 · Cited by 13 · see citing works
Self-citation
Hyalectanase Activities by the ADAMTS Metalloproteases
Fontanil, T., Mohamedi, Y., Casado, J. E., Obaya, Á. J., Cobo, T., & Cal, S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22062988 · Cited by 13 · see citing works
“This hyalectan is involved in physiological processes affecting brain plasticity, and it is located in perineuronal nets and in the axon initial segment [120].”
Background
Partial microglial depletion through inhibition of colony-stimulating factor 1 receptor improves synaptic plasticity and cognitive performance in aged mice
Strackeljan, L., Baidoe‐Ansah, D., Mirzapourdelavar, H., Jia, S., Kaushik, R., Cangalaya, C., & Dityatev, A. E. (2025) · Experimental Neurology · doi:10.1016/j.expneurol.2025.115186 · Cited by 14 · see citing works
Spatiotemporal distribution of proteoglycans in the developing rat's barrel field and the effects of early deafferentation
Bahia, C. P., Houzel, J., Diniz, C. W. P., & Pereira, A. (2008) · The Journal of Comparative Neurology · doi:10.1002/cne.21781 · Cited by 13 · see citing works
“The extracellular matrix (EM) of the brain is a rich source of a class of macromolecules known as proteoglycans, which are composed of glycosaminoglycan chains covalently bound to a protein core (Cooper and Steindler, 1986b; Ruoslahti, 1996; Rauch, 1997; John et al., 2006).”
Background
Tackling Obstacles for Gene Therapy Targeting Neurons: Disrupting Perineural Nets with Hyaluronidase Improves Transduction
Wanisch, K., Kovac, S., & Schorge, S. (2013) · PLoS ONE · doi:10.1371/journal.pone.0053269 · Cited by 11 · see citing works
“It has been shown that between week 2 and 3 in vitro cultures start to build up the ECM which is similar to that of the adult brain and mainly based on HA and chondroitin sulphate proteoglycans, [45].”
The effect of pharmacological inhibition of Serine Proteases on neuronal networks in vitro
Vijver, S. V. D., Missault, S., Soom, J. V., der Veken, P. V., Augustyns, K., Joossens, J., Dedeurwaerdere, S., & Giugliano, M. (2019) · PeerJ · doi:10.7717/peerj.6796 · Cited by 10 · see citing works
“We observed significant alterations in the collective emergent activity in vitro within just 1 h following the application of the SP inhibitor, consistent with the rapid in vivo action of ECM proteases (i.e., over minutes to hours (Wójtowicz, Brzdak & Mozrzymas, 2015)).”
BackgroundInfluential
Under the ECM Dome: The Physiological Role of the Perinodal Extracellular Matrix as an Ion Diffusion Barrier
Bekku, Y., & Oohashi, T. (2019) · Advances in experimental medicine and biology · doi:10.1007/978-981-32-9636-7_8 · Cited by 8 · see citing works
Astrocyte‐derived factors regulate CNS myelination
Seiler, S., Rudolf, F., Gomes, F. R., Pavlovic, A., Nebel, J., Seidenbecher, C. I., & Foo, L. C. (2024) · Glia · doi:10.1002/glia.24596 · Cited by 6 · see citing works
“…mouse anti-SMI31 (Biolegend 801,601), 1:1000 rabbit anti-CASPR (Invitrogen PA5-68409), 1:500 guinea pig anti-BCAN was previously described by (John et al., 2006), 1:1000 goat anti-CNTN1 (EIAab P15020), 1:1000 rabbit anti-GFAP (DAKO Z0334), 1:1000 mouse anti-NF200 (Sigma N4142), 1:500 rabbit…”
Self-citation
Dynamic Organization of Neuronal Extracellular Matrix Revealed by HaloTag-HAPLN1
Sterin, I., Niazi, A., Kim, J. S., Park, J., & Park, S. (2024) · Journal of Neuroscience · doi:10.1523/jneurosci.0666-24.2024 · Cited by 9 · see citing works
Chondroitin Sulphate Proteoglycan Axonal Coats in the Human Mediodorsal Thalamic Nucleus
Pantazopoulos, H., Hossain, N. M., Chelini, G., Durning, P. T., Barbas, H., Zikopoulos, B., & Berretta, S. (2022) · Frontiers in Integrative Neuroscience · doi:10.3389/fnint.2022.934764 · Cited by 5 · see citing works
An in vitro neuronal model replicating the in vivo maturation and heterogeneity of perineuronal netsPreprint
Dickens, S., Goodenough, A., & Kwok, J. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.01.22.477344 · Cited by 5 · see citing works
Extracellular baskets in inner hair cells and perineuronal nets in auditory nerves: Changes in noise-induced hearing loss rats
Kim, S. Y., Jeon, J. C., Park, B., & Kim, D. E. (2025) · Neuroscience Letters · doi:10.1016/j.neulet.2025.138147 · Cited by 4 · see citing works
Activity-dependent extracellular proteolytic cascade cleaves the ECM component brevican to promote structural plasticity
Singh, J. B., Perelló‐Amorós, B., Schneeberg, J., Mirzapourdelavar, H., Seidenbecher, C. I., Fejtová, A., Dityatev, A. E., & Frischknecht, R. (2025) · EMBO Reports · doi:10.1038/s44319-025-00644-w · Cited by 5 · see citing works
“Astrocytes play a critical role in synaptic plasticity and regulate several ECM proteins, including brevican (Favuzzi et al, 2017; Hamel et al, 2005; John et al, 2006).”
Self-citation
A New Interpretative Paradigm for Conformational Protein Diseases
Agnati, L. F., Guidolin, D., Woods, A. S., Ciruela, F., Carone, C., Vallelunga, A., Escuela, D. O. B., Genedani, S., & Fuxé, K. (2013) · Current Protein and Peptide Science · doi:10.2174/1389203711314020006 · Cited by 4 · see citing works
Reduced synaptic tagging by complement protein C3 is associated with elevated extracellular matrix in the middle-aged cerebellum of mice
Düsedau, H. P., Cangalaya, C., Stoyanov, S., Dityatev, A. E., & Dunay, I. R. (2025) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2025.1616390 · Cited by 3 · see citing works
Cell-generated mechanical forces play a role in epileptogenesis after injury
Dalir, L., Tatic-Lucic, S., & Berdichevsky, Y. (2025) · bioRxiv · doi:10.1016/j.expneurol.2025.115376 · Cited by 2 · see citing works
“However, production of new ECM near the cells (John et al., 2006) could be expected to push beads away from the slice, while matrix degradation by matrix metalloproteases should result in release of beads into the culture medium.”
Cerebellar Resistance to Amyloid Plaque Deposition and Elevated Microglial ECM Proteoglycan Uptake in 5xFAD Mice
Cangalaya, C., Düsedau, H. P., Dunay, I. R., Dityatev, A. E., & Stoyanov, S. (2026) · Cells · doi:10.3390/cells15020182 · Cited by 2 · see citing works
“…#019-19741, 1:500; rat anti-CD68, Bio-Rad, Hercules, CA, USA, #MCA1957, 1:500; chicken anti-VGLUT1, Synaptic Systems, Göttingen, Germany, #135316, 1:1000; rabbit anti-aggrecan, Merck Millipore, Darmstadt, Germany, #AB1031, 1:1000; guinea pig anti-brevican, John et al., 2006 [23]) for 48 h at 4 ◦ C.”

Deconstructing the perineuronal net: Cellular contributions and molecular composition of the neuronal extracellular matrix
Giamanco, K. A., & Matthews, R. T. (2012) · Neuroscience · doi:10.1016/j.neuroscience.2012.05.055 · Cited by 138 · see citing works
Brevican organizes hyaluronan-binding extracellular assemblies distinctively at the large diameter nodes of Ranvier in the CNS
Bekku, Y., Ninomiya, Y., & Oohashi, T. (2009) · Neuroscience Research · doi:10.1016/j.neures.2009.09.254
Perineuronal nets show intrinsic patterns of extracellular matrix differentiation in organotypic slice cultures
Grosche, J., & Br�ckner, G. (2001) · Experimental Brain Research · doi:10.1007/s002210000617 · Cited by 50 · see citing works
Brevican isoforms associate with neural membranes
Seidenbecher, C. I., Smalla, K., Fischer, N., Gundelfinger, E. D., & Kreutz, M. R. (2002) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2002.01183.x · Cited by 55 · see citing works
Self-citation
An in vitro neuronal model replicating the in vivo maturation and heterogeneity of perineuronal netsPreprint
Dickens, S., Goodenough, A., & Kwok, J. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.01.22.477344 · Cited by 5 · see citing works

Authors: John, N., Krügel, H., Frischknecht, R., Smalla, K.-H., Schultz, C., Kreutz, M. R., Gundelfinger, E. D., & Seidenbecher, C. I.
Affiliations: Leibniz Institute for Neurobiology (DE)
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI) (DE)
Goethe University Frankfurt (DE)
Corresponding author: Seidenbecher, Constanze I.
ORCID: John, Nora
Frischknecht, Renato
Smalla, Karl-Heinz
Schultz, Christian
Kreutz, Michael R.
Gundelfinger, Eckart D.
Seidenbecher, Constanze I.
Venue: Molecular and Cellular Neuroscience
Publisher: Elsevier BV
Published: 2006-04
Volume: 31
Issue: 4
Pages: 774-784
ISSN: 1044-7431 (ISSN-L)
DOI: 10.1016/j.mcn.2006.01.011
OpenAlex: W1965503179
Cited by: 122
References: 55
Citations by year: 2026: 4 · 2025: 8 · 2024: 6 · 2023: 2 · 2022: 4 · 2021: 7 · 2020: 4 · 2019: 8 · 2018: 5 · 2017: 6 · 2016: 2 · 2015: 10 · 2014: 6 · 2013: 5 · 2012: 7
FWCI: 2.89
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
doi.org/10.15223/policy-017 (stm-asf)
doi.org/10.15223/policy-037 (stm-asf)
doi.org/10.15223/policy-012 (stm-asf)
doi.org/10.15223/policy-029 (stm-asf)
doi.org/10.15223/policy-004 (stm-asf)
Locations: Molecular and Cellular Neuroscience (publishedVersion)
PubMed (publishedVersion)
Funders: Deutsche Forschungsgemeinschaft
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords: perineuronal nets, brevican, chondroitin sulfate proteoglycans, extracellular matrix, hippocampus, astrocytes, glial fibrillary acidic protein, synapses, axon initial segment, Vicia villosa lectin, primary neuronal cultures
MeSH terms: Animals, Cells, Cultured, Extracellular Matrix, Hippocampus, Humans, Monensin, Nerve Tissue Proteins, Neuroglia, Neurons, Chondroitin Sulfate Proteoglycans, Synapses, Biomarkers, Coculture Techniques, Protein Isoforms, Lectins, C-Type, Rats, Brevican

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Brevican-containing perineuronal nets of extracellular matrix in dissociated hippocampal primary cultures

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Cites

Cited by

Related

Record

About these checks