Quantitative Chemical Composition of Cortical GABAergic Neurons Revealed in Transgenic Venus-Expressing Rats
Abstract
Although neocortical GABAergic (gamma-aminobutyric acidergic) interneurons have been the focus of intense study, especially in the rat, a consensus view of the functional diversity and organization of inhibitory cortical neurons has not yet been achieved. To better analyze GABAergic neurons in the rat, we used a bacterial artificial chromosome (BAC) construct and established 2 lines of transgenic rats that coexpress Venus, a yellow fluorescent protein, with the vesicular GABA transporter. The brain GABA content from both transgenic lines was similar to the level found in wild-type rats. In the frontal cortex, Venus was expressed in >95% of GABAergic neurons, most of which also expressed at least one of 6 biochemical markers, including alpha-actitin-2, which preferentially labeled late-spiking neurogliaform cells. Taking advantage of the fact that Venus expression allows for targeted recording from all classes of nonpyramidal cells, irrespective of their somatic morphologies, we demonstrated that fast-spiking neurons, which were heterogeneous in somatic size as well as vertical dendritic projection, had relatively uniform horizontal dimensions, suggesting a cell type-specific columnar input territory. Our data demonstrate the benefits of VGAT-Venus rats for investigating GABAergic circuits, as well as the feasibility of using BAC technology in rats to label subsets of specific, genetically defined neurons.
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Dendrite targeting CCK-expressing interneurons are also found in the neocortex, most notably the vertically oriented bitufted double bouquet cells with ascending and descending tight radial axonal bundles targeting principal cell dendritic shafts and spines.
“Dendrite targeting CCK-expressing interneurons are also found in the neocortex, most notably the vertically oriented bitufted double bouquet cells with ascending and descending tight radial axonal bundles targeting principal cell dendritic shafts and spines (263, 355, 556, 569, 1034, 1085, 1150).”
From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1150]
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- Authors
- Uematsu, M., Hirai, Y., Karube, F., Ebihara, S., Kato, M., Abe, K., Obata, K., Yoshida, S., Hirabayashi, M., Yanagawa, Y., & Kawaguchi, Y.
- Affiliations
- Toyohashi University of Technology (JP)
- The Graduate University for Advanced Studies, SOKENDAI (JP)
- National Institute for Physiological Sciences (JP)
- RIKEN BioResource Research Center (JP)
- RIKEN (JP)
- Gunma University (JP)
- Venue
- Cerebral Cortex
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-05-20
- Volume
- 18
- Issue
- 2
- Pages
- 315-330
- ISSN
- 1047-3211 (ISSN-L)
- 1460-2199
- OpenAlex
- W2169652092
- Cited by
- 232
- References
- 60
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 3 · 2023: 10 · 2022: 8 · 2021: 15 · 2020: 13 · 2019: 15 · 2018: 17 · 2017: 19 · 2016: 19 · 2015: 14 · 2014: 13 · 2013: 17 · 2012: 11
- FWCI
- 3.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cerebral Cortex (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- GABAergic interneurons, neocortex, vesicular GABA transporter, Venus fluorescent protein, transgenic rats, bacterial artificial chromosome, fast-spiking neurons, neurogliaform cells, ACTN2, cell size, inhibitory circuits, frontal cortex
- MeSH terms
- Animals, Bacterial Proteins, Brain Mapping, Cerebral Cortex, gamma-Aminobutyric Acid, Interneurons, Luminescent Proteins, Microscopy, Fluorescence, Neural Inhibition, Synaptic Transmission, Tissue Distribution, Animals, Genetically Modified, Rats
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