Cited by 26Neuroscience and Neuropharmacology ResearchNeural dynamics and brain functionNeurogenesis and neuroplasticity mechanisms

Morphological and Functional Characterization of Non-fast-Spiking GABAergic Interneurons in Layer 4 Microcircuitry of Rat Barrel Cortex

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Emmenegger, Vishalini, Qi, Guanxiao, Wang, Haijun, Feldmeyer, Dirk·2018·Cerebral Cortex

Abstract

GABAergic interneurons are notorious for their heterogeneity, despite constituting a small fraction of the neuronal population in the neocortex. Classification of interneurons is crucial for understanding their widespread cortical functions as they provide a complex and dynamic network, balancing excitation and inhibition. Here, we investigated different types of non-fast-spiking (nFS) interneurons in Layer 4 (L4) of rat barrel cortex using whole-cell patch-clamp recordings with biocytin-filling. Based on a quantitative analysis on a combination of morphological and electrophysiological parameters, we identified 5 distinct types of L4 nFS interneurons: 1) trans-columnar projecting interneurons, 2) locally projecting non-Martinotti-like interneurons, 3) supra-granular projecting Martinotti-like interneurons, 4) intra-columnar projecting VIP-like interneurons, and 5) locally projecting neurogliaform-like interneurons. Trans-columnar projecting interneurons are one of the most striking interneuron types, which have not been described so far in Layer 4. They feature extensive axonal collateralization not only in their home barrel but also in adjacent barrels. Furthermore, we identified that most of the L4 nFS interneurons express somatostatin, while few are positive for the transcription factor Prox1. The morphological and electrophysiological characterization of different L4 nFS interneuron types presented here provides insights into their synaptic connectivity and functional role in cortical information processing.

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Crossref · 10.1093/cercor/bhx352

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  • Previous studies reported three types of fast-spiking (FS), parvalbumin-positive (PV+) interneurons and five types of non-FS interneurons, all with distinct morphologies.

    “Previous studies have reported three types of fast-spiking (FS), parvalbumin-positive (PV+) interneurons11 and five types of non-FS interneurons12, all of which have distinct morphologies.”

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  1. Ma Y, Hu H, Berrebi AS, Mathers PH, Agmon A 2006b. Distinct subtypes of somatostatin-containing neocortical interneurons revealed in transgenic mice. J Neurosci. 26: 5069– 5082. 16687498 PMC2020857

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Authors
Emmenegger, V., Qi, G., Wang, H., & Feldmeyer, D.
Affiliations
Forschungszentrum Jülich (DE)
Corresponding author
Feldmeyer, Dirk
Venue
Cerebral Cortex
Published
2018-01-10
Volume
28
Issue
4
Pages
1439-1457
ISSN
  • 1047-3211 (ISSN-L)
  • 1460-2199
OpenAlex
W2751409073
Cited by
26
References
110
Citations by year
2026: 1 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 5 · 2019: 3 · 2018: 2
FWCI
1.34
Type
journal article
Language
en
Open access
Locations
  • Cerebral Cortex (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • RWTH Publications (RWTH Aachen) (submittedVersion, other-oa)
  • Repository for Publications and Research Data (ETH Zurich) (submittedVersion, cc-by-nc)
  • ETH Zürich Research Collection
Funders
  • Helmholtz Society, the DFG Research Group
  • DFG International Research Training Group (1328)
  • Natural Science Fund for Colleges
  • Universities in the Jiangsu (14KJB140007)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
GABAergic interneurons, barrel cortex, layer 4, interneuron classification, somatostatin, Prox1, synaptic connectivity, cortical microcircuitry
MeSH terms
Animals, Animals, Newborn, Axons, Cerebral Cortex, Dendrites, Female, Lysine, Male, Membrane Potentials, Models, Neurological, Nerve Net, Vasoactive Intestinal Peptide, Patch-Clamp Techniques, Imaging, Three-Dimensional, Principal Component Analysis, Rats, GABAergic Neurons, In Vitro Techniques

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