Proteomic analysis of hyperadhesive Candida glabrata clinical isolates reveals a core wall proteome and differential incorporation of adhesins
Abstract
Attachment to human host tissues or abiotic medical devices is a key step in the development of infections by Candida glabrata. The genome of this pathogenic yeast codes for a large number of adhesins, but proteomic work using reference strains has shown incorporation of only few adhesins in the cell wall. By making inventories of the wall proteomes of hyperadhesive clinical isolates and reference strain CBS138 using mass spectrometry, we describe the cell wall proteome of C. glabrata and tested the hypothesis that hyperadhesive isolates display differential incorporation of adhesins. Two clinical strains (PEU382 and PEU427) were selected, which both were hyperadhesive to polystyrene and showed high surface hydrophobicity. Cell wall proteome analysis under biofilm-forming conditions identified a core proteome of about 20 proteins present in all C. glabrata strains. In addition, 12 adhesin-like wall proteins were identified in the hyperadherent strains, including six novel adhesins (Awp8-13) of which only Awp12 was also present in CBS138. We conclude that the hyperadhesive capacity of these two clinical C. glabrata isolates is correlated with increased and differential incorporation of cell wall adhesins. Future studies should elucidate the role of the identified proteins in the establishment of C. glabrata infections.
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C. glabrata clinical isolates have varied numbers of adhesin-encoding genes and a distinct profile of cell wall proteins expressed at the cell surface.
“Moreover, C. glabrata clinical isolates are known to have varied number of adhesin-encoding genes, and a distinct profile of cell wall proteins expressed at the cell surface [41,48,49].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [41]
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- Authors
- Gómez-Molero, E., de Boer, A. D., Dekker, H. L., Moreno-Martínez, A., Kraneveld, E. A., Ichsan, Chauhan, N., Weig, M., de Soet, J. J., de Koster, C. G., Bader, O., & de Groot, P. W. J.
- Affiliations
- Universitätsmedizin Göttingen (DE)
- University of Amsterdam (NL)
- Rutgers, The State University of New Jersey (US)
- Venue
- FEMS Yeast Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-11-05
- Volume
- 15
- Issue
- 8
- Pages
- fov098
- ISSN
- 1567-1356 (ISSN-L)
- 1567-1364
- OpenAlex
- W2265623652
- Cited by
- 39
- References
- 44
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 3 · 2023: 1 · 2022: 5 · 2021: 7 · 2020: 3 · 2019: 5 · 2018: 4 · 2017: 4 · 2016: 3
- FWCI
- 2.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Yeast Research (publishedVersion)
- PubMed (publishedVersion)
- UvA-DARE (University of Amsterdam) (publishedVersion)
- Rutgers University Community Repository (Rutgers University) (submittedVersion)
- UvA-DARE (University of Amsterdam) (submittedVersion)
- GRO (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- Candida glabrata, cell wall proteome, adhesins, clinical isolates, biofilm formation, surface hydrophobicity, fungal virulence
- MeSH terms
- Candidiasis, Cell Wall, Fungal Proteins, Humans, Mass Spectrometry, Proteome, Proteomics, Candida glabrata
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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