Cited by 39Antifungal resistance and susceptibilityFungal and yeast genetics researchYeasts and Rust Fungi Studies

Proteomic analysis of hyperadhesive Candida glabrata clinical isolates reveals a core wall proteome and differential incorporation of adhesins

Gómez-Molero, Emilia, de Boer, Albert D., Dekker, Henk L., Moreno-Martínez, Ana, Kraneveld, Eef A., Ichsan, Chauhan, Neeraj, Weig, Michael +4 more·2015·FEMS Yeast Research

Abstract

Attachment to human host tissues or abiotic medical devices is a key step in the development of infections by Candida glabrata. The genome of this pathogenic yeast codes for a large number of adhesins, but proteomic work using reference strains has shown incorporation of only few adhesins in the cell wall. By making inventories of the wall proteomes of hyperadhesive clinical isolates and reference strain CBS138 using mass spectrometry, we describe the cell wall proteome of C. glabrata and tested the hypothesis that hyperadhesive isolates display differential incorporation of adhesins. Two clinical strains (PEU382 and PEU427) were selected, which both were hyperadhesive to polystyrene and showed high surface hydrophobicity. Cell wall proteome analysis under biofilm-forming conditions identified a core proteome of about 20 proteins present in all C. glabrata strains. In addition, 12 adhesin-like wall proteins were identified in the hyperadherent strains, including six novel adhesins (Awp8-13) of which only Awp12 was also present in CBS138. We conclude that the hyperadhesive capacity of these two clinical C. glabrata isolates is correlated with increased and differential incorporation of cell wall adhesins. Future studies should elucidate the role of the identified proteins in the establishment of C. glabrata infections.

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Crossref · 10.1093/femsyr/fov098

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  • C. glabrata clinical isolates have varied numbers of adhesin-encoding genes and a distinct profile of cell wall proteins expressed at the cell surface.

    “Moreover, C. glabrata clinical isolates are known to have varied number of adhesin-encoding genes, and a distinct profile of cell wall proteins expressed at the cell surface [41,48,49].”

    From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [41]

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Authors
Gómez-Molero, E., de Boer, A. D., Dekker, H. L., Moreno-Martínez, A., Kraneveld, E. A., Ichsan, Chauhan, N., Weig, M., de Soet, J. J., de Koster, C. G., Bader, O., & de Groot, P. W. J.
Affiliations
  • Universitätsmedizin Göttingen (DE)
  • University of Amsterdam (NL)
  • Rutgers, The State University of New Jersey (US)
Venue
FEMS Yeast Research
Published
2015-11-05
Volume
15
Issue
8
Pages
fov098
ISSN
  • 1567-1356 (ISSN-L)
  • 1567-1364
OpenAlex
W2265623652
Cited by
39
References
44
Citations by year
2026: 3 · 2025: 1 · 2024: 3 · 2023: 1 · 2022: 5 · 2021: 7 · 2020: 3 · 2019: 5 · 2018: 4 · 2017: 4 · 2016: 3
FWCI
2.86
Type
journal article
Language
en
Open access
closed
Locations
  • FEMS Yeast Research (publishedVersion)
  • PubMed (publishedVersion)
  • UvA-DARE (University of Amsterdam) (publishedVersion)
  • Rutgers University Community Repository (Rutgers University) (submittedVersion)
  • UvA-DARE (University of Amsterdam) (submittedVersion)
  • GRO (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
Keywords
Candida glabrata, cell wall proteome, adhesins, clinical isolates, biofilm formation, surface hydrophobicity, fungal virulence
MeSH terms
Candidiasis, Cell Wall, Fungal Proteins, Humans, Mass Spectrometry, Proteome, Proteomics, Candida glabrata

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