Cited by 195Marine Invertebrate Physiology and EcologyDevelopmental Biology and Gene RegulationProtist diversity and phylogeny

Unexpected repertoire of metazoan transcription factors in the unicellular holozoan Capsaspora owczarzaki

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Sebe-Pedros, A., de Mendoza, A., Lang, B. F., Degnan, B. M., Ruiz-Trillo, I.·2011·Molecular Biology and Evolution

Abstract

How animals (metazoans) originated from their single-celled ancestors remains a major question in biology. As transcriptional regulation is crucial to animal development, deciphering the early evolution of associated transcription factors (TFs) is critical to understanding metazoan origins. In this study, we uncovered the repertoire of 17 metazoan TFs in the amoeboid holozoan Capsaspora owczarzaki, a representative of a unicellular lineage that is closely related to choanoflagellates and metazoans. Phylogenetic and comparative genomic analyses with the broadest possible taxonomic sampling allowed us to formulate new hypotheses regarding the origin and evolution of developmental metazoan TFs. We show that the complexity of the TF repertoire in C. owczarzaki is strikingly high, pushing back further the origin of some TFs formerly thought to be metazoan specific, such as T-box or Runx. Nonetheless, TF families whose beginnings antedate the origin of the animal kingdom, such as homeodomain or basic helix-loop-helix, underwent significant expansion and diversification along metazoan and eumetazoan stems.

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Crossref · 10.1093/molbev/msq309

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Authors
Sebe-Pedros, A., de Mendoza, A., Lang, B. F., Degnan, B. M., & Ruiz-Trillo, I.
Affiliations
  • Université de Montréal (CA)
  • The University of Queensland (AU)
Venue
Molecular Biology and Evolution
Published
2010-11-17
Volume
28
Issue
3
Pages
1241-1254
ISSN
  • 0737-4038 (ISSN-L)
  • 1537-1719
OpenAlex
W2108650715
Cited by
195
References
97
Citations by year
2026: 5 · 2025: 6 · 2024: 8 · 2023: 7 · 2022: 9 · 2021: 8 · 2020: 6 · 2019: 11 · 2018: 12 · 2017: 16 · 2016: 20 · 2015: 27 · 2014: 12 · 2013: 19 · 2012: 19
FWCI
10.79
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology and Evolution (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Harvard University
  • Broad Institute
  • Institució Catalana de Recerca i Estudis Avançats
  • Ministerio de Ciencia e Innovación (ERC-2007-StG-206883)
Topics
  • Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protist diversity and phylogeny
Keywords
Capsaspora owczarzaki, transcription factors, metazoan origin, T-box, RUNX, homeodomain, bHLH, transcription factor evolution, gene family expansion, choanoflagellates, transcriptional regulation
MeSH terms
Amino Acid Sequence, Animals, DNA-Binding Proteins, Molecular Sequence Data, Phylogeny, Transcription Factors, Evolution, Molecular, Genomics, Choanoflagellata

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