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PreprintCited by 8Barrier Structure and Function StudiesLysosomal Storage Disorders ResearchNeuroinflammation and Neurodegeneration Mechanisms

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Unraveling the link between Neuropathy Target Esterase NTE/SWS, lysosomal storage diseases, inflammation, and abnormal fatty acid metabolism

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Tsap, Mariana I., Yatsenko, Andriy S., Hegermann, Jan, Beckmann, Bibiana, Tsikas, Dimitrios, Shcherbata, Halyna R.·2023·eLife
DOI 10.1101/2023.08.11.552934PMC PMC11090517

Preprint — published as 10.7554/elife.98020 · Paper page

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Abstract

Mutations in Drosophila Swiss Cheese (SWS) gene or its vertebrate orthologue Neuropathy Target Esterase (NTE) lead to progressive neuronal degeneration in flies and humans. Despite its enzymatic function as a phospholipase is well-established, the molecular mechanism responsible for maintaining nervous system integrity remains unclear. In this study, we found that SWS is present in surface glia that form the blood-brain-barrier (BBB) and that SWS is important to maintain its structure and permeability. Importantly, BBB glia-specific expression of Drosophila SWS or human NTE in the sws mutant background fully rescues surface glial organization and partially restores BBB integrity, suggesting a conserved function of NTE/SWS. Interestingly, sws mutant glia showed abnormal organization of plasma membrane domains and tight junction rafts accompanied by the accumulation of lipid droplets, lysosomes, and multilamellar bodies. Since the observed cellular phenotypes closely resemble the characteristics described in a group of metabolic disorders known as lysosomal storage diseases (LSDs), our data established a novel connection between NTE/SWS and these conditions. We found that mutants with defective BBB exhibit elevated levels of fatty acids, which are precursors of eicosanoids and are involved in the inflammatory response. Also, as a consequence of a permeable BBB, several innate immunity factors are upregulated. Treatment with anti-inflammatory agents prevents the abnormal architecture of the BBB, suggesting that inflammation contributes to the maintenance of a healthy brain barrier. Since a defective BBB is associated with many neurodegenerative diseases, a better understanding of the molecular mechanisms of inflammation may help to promote the use of anti-inflammatory therapy for age-related neurodegeneration.

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Metadata

Authors
Tsap, M. I., Yatsenko, A. S., Hegermann, J., Beckmann, B., Tsikas, D., & Shcherbata, H. R.
Affiliations
  • Medizinische Hochschule Hannover (DE)
  • Mount Desert Island Biological Laboratory (US)
Corresponding author
Shcherbata, Halyna R.
ORCID
Venue
eLife
Publisher
openRxiv
Published
2023-08-12
Volume
13
ISSN
2050-084X (ISSN-L)
DOI
10.1101/2023.08.11.552934
PMC
PMC11090517
OpenAlex
W4395454199
Cited by
8
References
1
Citations by year
2026: 3 · 2025: 5
FWCI
1.24
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • eLife (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • European Molecular Biology Organization
  • Deutsche Forschungsgemeinschaft (INST 192/574-1 FUGG, 521749003)
  • Volkswagen Foundation (90218, 97750)
  • National Institutes of Health
  • National Institute of General Medical Sciences (P20GM104318, P20GM103423)
Topics
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Health Sciences › Medicine › Genetics (clinical) › Lysosomal Storage Disorders Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
neuropathy target esterase, blood-brain barrier, lysosomal storage disease, fatty acid metabolism, eicosanoids, neuroinflammation, innate immunity, lipid droplets, multilamellar bodies, plasma membrane domains, age-related neurodegeneration, anti-inflammatory therapy, Drosophila
MeSH terms
Animals, Blood-Brain Barrier, Carboxylic Ester Hydrolases, Drosophila melanogaster, Fatty Acids, Humans, Inflammation, Neuroglia, Lysosomal Storage Diseases, Drosophila Proteins

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