Cited by 183Muscle Physiology and DisordersAdipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and Metabolism

Myostatin, activin receptor IIb, and follistatin-like-3 gene expression are altered in adipose tissue and skeletal muscle of obese mice

Allen, David L., Cleary, Allison S., Speaker, Kristin J., Lindsay, Sarah F., Uyenishi, Jill, Reed, Jason M., Madden, Molly C., Mehan, Ryan S.·2008·American Journal of Physiology-Endocrinology and Metabolism

Abstract

Myostatin (MSTN) is a secreted growth inhibitor expressed in muscle and adipose. We sought to determine whether expression of MSTN, its receptor activin RIIb (ActRIIb), or its binding protein follistatin-like-3 (FSTL3) are altered in subcutaneous or visceral adipose or in skeletal muscle in response to obesity. MSTN and ActRIIb mRNA levels were low in subcutaneous (SQF) and visceral fat (VF) from wild-type mice but were 50- to 100-fold higher in both SQF and VF from ob/ob compared with wild-type mice. FSTL3 mRNA levels were increased in SQF but decreased in VF in ob/ob compared with wild-type mice. Moreover, MSTN mRNA levels were twofold greater in tibialis anterior (TA) from ob/ob mice, whereas ActRIIb and FSTL3 mRNA levels were unchanged. MSTN mRNA levels were also increased in TA and SQF from mice on a high-fat diet. Injection of ob/ob mice with recombinant leptin caused FSTL3 mRNA levels to decrease in both VF and SQF in ob/ob mice; MSTN and ActRIIb mRNA levels tended to decrease only in VF. Finally, MSTN mRNA levels and promoter activity were low in adipogenic 3T3-L1 cells, but an MSTN promoter-reporter construct was activated in 3T3-L1 cells by cotransfection with the adipogenic transcription factors SREBP-1c, C/EBPalpha, and PPARgamma. These results demonstrate that expression of MSTN and its associated binding proteins can be modulated in adipose tissue and skeletal muscle by chronic obesity and suggest that alterations in their expression may contribute to the changes in growth and metabolism of lean and fat tissues occurring during obesity.

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Crossref · 10.1152/ajpendo.00798.2007

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Authors
Allen, D. L., Cleary, A. S., Speaker, K. J., Lindsay, S. F., Uyenishi, J., Reed, J. M., Madden, M. C., & Mehan, R. S.
Affiliations
University of Colorado Boulder (US)
Venue
American Journal of Physiology-Endocrinology and Metabolism
Published
2008-05
Volume
294
Issue
5
Pages
E918-E927
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
OpenAlex
W1986156265
Cited by
183
References
50
Citations by year
2026: 3 · 2025: 5 · 2024: 7 · 2023: 5 · 2022: 11 · 2021: 11 · 2020: 8 · 2019: 10 · 2018: 9 · 2017: 8 · 2016: 8 · 2015: 17 · 2014: 18 · 2013: 17 · 2012: 14
FWCI
4.38
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Arthritis and Musculoskeletal and Skin Diseases (K01 AR-0505-01)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
myostatin, FSTL3, obesity, adipose tissue, skeletal muscle, subcutaneous adipose tissue, visceral fat, ob/ob mice, leptin, high-fat diet, adipogenic transcription factors, SREBP-1c, C/EBPα, PPARγ, 3T3-L1 cells, gene expression regulation
MeSH terms
Adipose Tissue, Animals, Cells, Cultured, Cloning, Molecular, Diet, Dietary Fats, DNA, Male, Mice, Inbred C57BL, Mice, Obese, Obesity, Proteins, RNA, Messenger, Transfection, Signal Transduction, Transforming Growth Factor beta, 3T3 Cells, Muscle, Skeletal, Reverse Transcriptase Polymerase Chain Reaction, Leptin, Activin Receptors, Type II, Follistatin-Related Proteins, Mice, Myostatin

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