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Hippocampal CA1 interneurons: an in vivo intracellular labeling study

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Sik, A, Penttonen, M, Ylinen, A, Buzsaki, G·1995·The Journal of Neuroscience
DOI 10.1523/jneurosci.15-10-06651.1995PMC PMC6577981
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Abstract

Fast spiking interneurons in the CA1 area of the dorsal hippocampus were recorded from and filled with biocytin in anesthetized rats. The full extent of their dendrites and axonal arborizations as well as their calcium binding protein content were examined. Based on the spatial extent of axon collaterals, local circuit cells (basket and O- LM neurons) and long-range cells (bistratified, trilaminar, and backprojection neurons) could be distinguished. Basket cells were immunoreactive for parvalbumin and their axon collaterals were confined to the pyramidal layer. A single basket cell contacted more than 1500 pyramidal neurons and 60 other parvalbumin-positive interneurons. Commissural stimulation directly discharged basket cells, followed by an early and late IPSPs, indicating interneuronal inhibition of basket cells. The dendrites of another local circuit neuron (O-LM) were confined to stratum oriens and it had a small but high-density axonal terminal field in stratum lacunosum-moleculare. The fastest firing cell of all interneurons was a calbindin-immunoreactive bistratified neuron with axonal targets in stratum oriens and radiatum. Two neurons with their cell bodies in the alveus innervated the CA3 region (backprojection cells), in addition to rich axon collaterals in the CA1 region. The trilaminar interneuron had axon collaterals in strata radiatum, oriens and pyramidale with its dendrites confined to stratum oriens. Commissural stimulation evoked an early EPSP-IPSP-late depolarizing potential sequence in this cell. All interneurons formed symmetric synapses with their targets at the electron microscopic level. These findings indicate that interneurons with distinct axonal targets have differential functions in shaping the physiological patterns of the CA1 network.

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Crossref · 10.1523/jneurosci.15-10-06651.1995

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  • PV-INs connect to many principal neurons.

    “In addition, PV-INs connect to many principal neurons [74,75,87,88].”

    From Parvalbumin interneuron vulnerability and brain disorders (2021) · cites this paper as [88]

  • PVBCs predominantly display mostly aspiny, pyramidal-shaped, or bitufted dendritic trees spanning from the alveus to stratum lacunosum moleculare, positioning them to receive input from all excitatory afferent projections innervating the hippocampal CA1 region.

    “PVBCs predominantly display mostly aspiny, pyramidal-shaped, or bitufted dendritic trees spanning from the alveus to s.l.m., positioning them to receive input from all excitatory afferent projections innervating the hippocampal CA1 region (FIGURE 2C) (135, 768, 1014).”

    From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1014]

  • O-LMs are named for their striking anatomy with soma and dendrites restricted to s.o. and the alveus while the axon ascends with minimal branching through s.p. and s.r. to densely collateralize within s.l.m.

    “O-LMs are named for their striking anatomy with soma and dendrites restricted to s.o. and the alveus while the axon ascends with minimal branching through s.p. and s.r. to densely collateralize within s.l.m. (FIGURE 3C) (352, 719, 721, 768, 1014).”

    From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1014]

  • After emerging from the soma or a proximal dendrite, ~7% of an O-LM axon remains in s.o. while greater than 90% is targeted to s.l.m.

    “After emerging from the soma or a proximal dendrite, ~7% of an O-LM axon remains in s.o. while greater than 90% is targeted to s.l.m. (1014).”

    From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1014]

  • Individual O-LMs are estimated to contact around 1,450 pyramidal cells with an average of 10 synapses per connection.

    “Individual O-LMs are estimated to contact around 1,450 pyramidal cells with an average of 10 synapses per connection (102, 1014).”

    From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1014]

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Authors
Sik, A., Penttonen, M., Ylinen, A., & Buzsaki, G.
Affiliations
Rutgers, The State University of New Jersey (US)
ORCID
Venue
The Journal of Neuroscience
Publisher
Society for Neuroscience
Published
1995-10-01
Volume
15
Issue
10
Pages
6651-6665
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
DOI
10.1523/jneurosci.15-10-06651.1995
PMC
PMC6577981
OpenAlex
W1568625389
Cited by
669
References
62
Citations by year
2026: 3 · 2025: 18 · 2024: 10 · 2023: 12 · 2022: 15 · 2021: 11 · 2020: 26 · 2019: 26 · 2018: 23 · 2017: 27 · 2016: 29 · 2015: 18 · 2014: 27 · 2013: 14 · 2012: 23
FWCI
12.66
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • U.S. Public Health Service (34994)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
hippocampal CA1, fast-spiking interneurons, basket cells, parvalbumin, calbindin, axon collaterals, dendritic branching, inhibitory postsynaptic potentials, local circuit neurons, synaptic ultrastructure
MeSH terms
Animals, Axons, Electrophysiology, Hippocampus, Interneurons, Intracellular Membranes, Lysine, Microscopy, Electron, Nerve Tissue Proteins, Parvalbumins, Rats, Sprague-Dawley, Rats, Calbindins, S100 Calcium Binding Protein G

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