Candida glabrata displays pseudohyphal growth
Open accessAbstract
The ability to undergo morphological change has been reported as an advantageous trait in fungal pathogenesis. Here we demonstrate that Candida glabrata ATCC2001, like diploid Saccharomyces cerevisiae strains, forms elongated chains of pseudohyphal cells on solid nitrogen starvation media (SLAD). Constrictions were apparent between adjoining cells; no parallel-sided hyphae were seen and pseudohyphae invaded the agar. When SLAD was supplemented with ammonium sulfate both C. glabrata and diploid S. cerevisiae strains lost their ability to undergo pseudohyphal growth. However, on this media C. glabrata yeast cells invaded the agar in a similar fashion to the invasive growth mode exhibited by haploid strains of S. cerevisiae cultured on rich media such as YPD. C. glabrata was not capable of invading YPD demonstrating that the process of filamentation is distinct in these two fungi. To our knowledge this is the first report to demonstrate that C. glabrata can undergo morphological change and grow as an invasive filamentous organism.
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C. glabrata displayed pseudohyphal structures in response to nitrogen starvation and carbon dioxide exposure.
“Importantly, morphological switching from the yeast to the hyphal form has not been reported [28], although C. glabrata displayed pseudohyphal structures in response to nitrogen starvation and carbon dioxide exposure [70,71].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [70]
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- Authors
- Csank, C., & Haynes, K.
- Affiliations
- Biotechnology Research Institute (CA)
- Imperial College London (GB)
- Corresponding author
- Haynes, Ken
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-08
- Volume
- 189
- Issue
- 1
- Pages
- 115-120
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W1996364851
- Cited by
- 129
- References
- 28
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 3 · 2023: 4 · 2022: 6 · 2021: 3 · 2020: 6 · 2019: 7 · 2018: 3 · 2017: 5 · 2016: 3 · 2015: 10 · 2014: 13 · 2013: 4 · 2012: 6
- FWCI
- 1.45
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- NPARC (submittedVersion)
- INFM-OAR (INFN Catania) (submittedVersion, other-oa)
- Funders
- Medical Research Council Canada
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- Candida glabrata, pseudohyphal growth, filamentation, morphological switching, nitrogen starvation, invasive growth, Saccharomyces cerevisiae, fungal pathogenesis, ammonium sulfate
- MeSH terms
- Candida, Candida albicans, Culture Media, Humans, Nitrogen, Saccharomyces cerevisiae
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