mVps34 is activated following high‐resistance contractions
Open accessAbstract
Following resistance exercise in the fasted state, both protein synthesis and degradation in skeletal muscle are increased. The addition of essential amino acids potentiates the synthetic response suggesting that an amino acid sensor, which is involved in both synthesis and degradation, may be activated by resistance exercise. One such candidate protein is the class 3 phosphatidylinositol 3OH‐kinase (PI3K) Vps34. To determine whether mammalian Vps34 (mVps34) is modulated by high‐resistance contractions, mVps34 and S6K1 (an index of mTORC1) activity were measured in the distal hindlimb muscles of rats 0.5, 3, 6 and 18 h after acute unilateral high‐resistance contractions with the contralateral muscles serving as a control. In the lengthening tibialis anterior (TA) muscle, S6K1 (0.5 h = 366.3 ± 112.08%, 3 h = 124.7 ± 15.96% and 6 h = 129.2 ± 0%) and mVps34 (3 h = 68.8 ± 15.1% and 6 h = 36.0 ± 8.79%) activity both increased, whereas in the shortening soleus and plantaris (PLN) muscles the increase was significantly lower (PLN S6K1 0.5 h = 33.1 ± 2.29% and 3 h = 47.0 ± 6.65%; mVps34 3 h = 24.5 ± 7.92%). HPLC analysis of the TA demonstrated a 25% increase in intramuscular leucine concentration in rats 1.5 h after exercise. A similar level of leucine added to C2C12 cells in vitro increased mVps34 activity 3.2‐fold. These data suggest that, following high‐resistance contractions, mVps34 activity is stimulated by an influx of essential amino acids such as leucine and this may prolong mTORC1 signalling and contribute to muscle hypertrophy.
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Following resistance exercise there is a significant and sustained increase in the activity of mTORC1, as determined by an increase in S6K phosphorylation and activity.
“Following resistance exercise there is a significant and sustained increase in the activity of mTORC1, as determined by an increase in S6K phosphorylation [8] and activity [9].”
From Using Molecular Biology to Maximize Concurrent Training (2014) · cites this paper as [9]
mTORC1 activity can be maintained for at least 18 h after resistance exercise.
“mTORC1 activity can be maintained for at least 18 h after resistance exercise [8, 9].”
From Using Molecular Biology to Maximize Concurrent Training (2014) · cites this paper as [9]
In the hours after resistance exercise there is an increase in the rate of amino acid uptake from the blood into the muscle.
“In the hours after resistance exercise there is also an increase in the rate of amino acid uptake from the blood into the muscle.”
From Using Molecular Biology to Maximize Concurrent Training (2014) · cites this paper as [9]
Leucine and glutamine are increased within the working muscle after resistance exercise.
“Specifically, leucine and glutamine are increased within the working muscle [9, 15].”
From Using Molecular Biology to Maximize Concurrent Training (2014) · cites this paper as [9]
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- Authors
- MacKenzie, M. G., Hamilton, D. L., Murray, J. T., Taylor, P. M., & Baar, K.
- Affiliations
- University of Dundee (GB)
- Queen's University Belfast (GB)
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2009-01
- Volume
- 587
- Issue
- 1
- Pages
- 253-260
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC2670038
- OpenAlex
- W2172067307
- Cited by
- 94
- References
- 35
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 4 · 2018: 7 · 2017: 6 · 2016: 5 · 2015: 9 · 2014: 12 · 2013: 6 · 2012: 3
- FWCI
- 4.38
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Discovery Research Portal (University of Dundee) (publishedVersion)
- Stirling Online Research Repository (University of Stirling) (submittedVersion, public-domain)
- Research Portal (Queen's University Belfast) (submittedVersion)
- Funders
- Wellcome Trust (077426)
- British Heart Foundation (FS/05/059)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Keywords
- mTORC1, S6K1, resistance training, leucine, essential amino acids, amino acid sensing, skeletal muscle protein synthesis, muscle protein degradation, muscle hypertrophy, tibialis anterior, soleus muscle, plantaris, C2C12 myoblasts
- MeSH terms
- Animals, Cell Line, Enzyme Activation, Physical Exertion, Female, Hypertrophy, Leucine, Muscle Contraction, Stress, Mechanical, Transcription Factors, Signal Transduction, Rats, Wistar, Muscle, Skeletal, Phosphatidylinositol 3-Kinases, Ribosomal Protein S6 Kinases, Mice, Rats, Resistance Training
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