CoChat
Sign Up
Cited by 207

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase–Cas1 fusion protein

Silas, Sukrit, Mohr, Georg, Sidote, David J., Markham, Laura M., Sanchez-Amat, Antonio, Bhaya, Devaki, Lambowitz, Alan M., Fire, Andrew Z.·2016·Science
DOI 10.1126/science.aad4234PMC PMC4898656
Open this paper in CoChat — free account

Abstract

CRISPR-Cas captures invading RNA: The CRISPR (clustered regularly interspaced short palindromic repeat) system provides bacteria with an adaptive immune response. DNA captured from viruses and plasmids by CRISPR-associated protein 1 (Cas1) is used by bacteria to target the invaders' for destruction. Silas et al. discover that certain classes of the Cas1 gene are fused to a reverse transcriptase gene ( RT-Cas1 ) (see the Perspective by Sontheimer and Marraffini). These RT-Cas1 proteins are able to capture and directly incorporate both DNA and RNA into CRISPR loci. RT-Cas1 systems could be effective against parasitic RNA species, or even to modulate bacterial gene expression. Science , this issue p. 10.1126/science.aad4234 ; see also p. 920

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1126/science.aad4234

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The type III-B Cas-system of Marinomonas mediterranea has Cas1 linked to a reverse transcriptase, meaning that spacers can be obtained from RNA-based invaders and subsequently reverse transcripted into DNA.

    “The type III-B Cas-system of Marinomonas mediterranea is particularly interesting because Cas1 is linked to a reverse transcriptase, meaning that spacers can be obtained from RNA-based invaders and subsequently reverse transcripted into DNA [31].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [31]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 47

  1. 10.1126/science.1138140 · DOI · Paper page
  2. 10.1038/nrg2749 · DOI · Paper page
  3. 10.1099/mic.0.28048-0 · DOI · Paper page
  4. 10.1007/s00239-004-0046-3 · DOI · Paper page
  5. 10.1099/mic.0.27437-0 · DOI · Paper page
  6. 10.1126/science.1159689 · DOI · Paper page
  7. 10.1038/nrmicro3279 · DOI
  8. 10.1038/nrmicro2577 · DOI · Paper page
  9. 10.1038/nrmicro3569 · DOI · Paper page
  10. 10.1186/1745-6150-1-7 · DOI · Paper page
  11. 10.1093/nar/gks216 · DOI · Paper page
  12. 10.1038/ncomms1937 · DOI · Paper page
  13. 10.1101/gad.257550.114 · DOI
  14. 10.1038/nature14245 · DOI
  15. 10.1126/science.1165771 · DOI · Paper page
  16. 10.1016/j.cell.2009.07.040 · DOI · Paper page
  17. 10.1016/j.molcel.2011.10.023 · DOI
  18. 10.1016/j.molcel.2014.09.027 · DOI
  19. 10.1038/nature13637 · DOI
  20. 10.1093/nar/gku1302 · DOI
  21. 10.1016/j.cell.2015.04.027 · DOI · Paper page
  22. 10.1016/0092-8674(85)90170-9 · DOI · Paper page
  23. 10.1016/0092-8674(85)90197-7 · DOI
  24. 10.1016/0092-8674(95)90027-6 · DOI
  25. 10.1126/science.1067467 · DOI
  26. 10.1093/molbev/msn081 · DOI
  27. 10.1093/nar/gkn867 · DOI
  28. 10.1371/journal.pone.0114083 · DOI
  29. 10.1093/oxfordjournals.molbev.a026164 · DOI
  30. 10.1261/rna.7181105 · DOI
  31. 10.1371/journal.pbio.1000391 · DOI
  32. 10.1099/00207713-49-3-1241 · DOI
  33. 10.1016/j.tibs.2004.01.002 · DOI
  34. 10.1016/j.bbrc.2013.10.122 · DOI
  35. 10.1101/SQB.1987.052.01.023 · DOI
  36. 10.1146/annurev.biochem.75.103004.142733 · DOI
  37. 10.1038/nature14237 · DOI · Paper page
  38. 10.1146/annurev.genet.38.072902.091600 · DOI
  39. 10.1146/annurev.bi.48.070179.002521 · DOI
  40. 10.1073/pnas.70.3.934 · DOI
  41. 10.1016/0092-8674(89)90917-3 · DOI
  42. 10.1146/annurev-micro-091313-103416 · DOI
  43. 10.1186/1471-2164-13-734 · DOI
  44. W. Ludwig H. P. Klenk in Bergey’s Manual of Systematic Bacteriology G. M. Garrity Ed. (Springer vol. 2 2001) pp. 49–65. · DOI
  45. 10.1261/rna.039743.113 · DOI
  46. 10.1128/JB.182.13.3754-3760.2000 · DOI
  47. 10.1038/nature14302 · DOI

Cited by

Sorted by citations

Metadata

Authors
Silas, S., Mohr, G., Sidote, D. J., Markham, L. M., Sanchez-Amat, A., Bhaya, D., Lambowitz, A. M., & Fire, A. Z.
Affiliations
  • Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas–Austin, Austin, TX 78712, USA.
  • Department of Genetics and Microbiology, Universidad de Murcia, Murcia 30100, Spain.
  • Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA.
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2016-02-26
Volume
351
Issue
6276
ISSN
  • 0036-8075
  • 1095-9203
DOI
10.1126/science.aad4234
PMC
PMC4898656
Cited by
207
References
3
Language
en
Funders
  • Howard Hughes Medical Institute
  • NIH (R01-GM37706, R01-GM37949, R01-GM37951)
  • Welch Foundation (F-1607)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review