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Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases

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Clarridge, Jill E.·2004·Clinical Microbiology Reviews
DOI 10.1128/cmr.17.4.840-862.2004
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Abstract

SUMMARY: The traditional identification of bacteria on the basis of phenotypic characteristics is generally not as accurate as identification based on genotypic methods. Comparison of the bacterial 16S rRNA gene sequence has emerged as a preferred genetic technique. 16S rRNA gene sequence analysis can better identify poorly described, rarely isolated, or phenotypically aberrant strains, can be routinely used for identification of mycobacteria, and can lead to the recognition of novel pathogens and noncultured bacteria. Problems remain in that the sequences in some databases are not accurate, there is no consensus quantitative definition of genus or species based on 16S rRNA gene sequence data, the proliferation of species names based on minimal genetic and phenotypic differences raises communication difficulties, and microheterogeneity in 16S rRNA gene sequence within a species is common. Despite its accuracy, 16S rRNA gene sequence analysis lacks widespread use beyond the large and reference laboratories because of technical and cost considerations. Thus, a future challenge is to translate information from 16S rRNA gene sequencing into convenient biochemical testing schemes, making the accuracy of the genotypic identification available to the smaller and routine clinical microbiology laboratories.

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Metadata

Authors
Clarridge, J. E.
Venue
Clinical Microbiology Reviews
Publisher
American Society for Microbiology
Published
2004-10
Volume
17
Issue
4
Pages
840-862
ISSN
  • 0893-8512 (ISSN-L)
  • 1098-6618
DOI
10.1128/cmr.17.4.840-862.2004
OpenAlex
W2133371096
Cited by
2,148
References
7
FWCI
17.84
Type
journal article
Language
en
Open access
bronze
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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Clarridge, J. E. (2004). Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases. Clinical Microbiology Reviews. https://doi.org/10.1128/cmr.17.4.840-862.2004

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