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ADD1/SREBP-1c Is Required in the Activation of Hepatic Lipogenic Gene Expression by Glucose
Open accessAbstract
The transcription of genes encoding proteins involved in the hepatic synthesis of lipids from glucose is strongly stimulated by carbohydrate feeding. It is now well established that in the liver, glucose is the main activator of the expression of this group of genes, with insulin having only a permissive role. While ADD1/SREBP-1 has been implicated in lipogenic gene expression through temporal association with food intake and ectopic gain-of-function experiments, no genetic evidence for a requirement for this factor in glucose-mediated gene expression has been established. We show here that the transcription of ADD1/SREBP-1c in primary cultures of hepatocytes is controlled positively by insulin and negatively by glucagon and cyclic AMP, establishing a link between this transcription factor and carbohydrate availability. Using adenovirus-mediated transfection of a powerful dominant negative form of ADD1/SREBP-1c in rat hepatocytes, we demonstrate that this factor is absolutely necessary for the stimulation by glucose of L-pyruvate kinase, fatty acid synthase, S14, and acetyl coenzyme A carboxylase gene expression. These results demonstrate that ADD1/SREBP-1c plays a crucial role in mediating the expression of lipogenic genes induced by glucose and insulin.
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Saltiel, A. R., & Kahn, C. R. (2001) · Nature · doi:10.1038/414799a · Cited by 5,326
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Horton, J. D., Goldstein, J. L., & Brown, M. S. (2002) · Journal of Clinical Investigation · doi:10.1172/jci15593 · Cited by 4,794
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Kersten, S., Desvergne, B., & Wahli, W. (2000) · Nature · doi:10.1038/35013000 · Cited by 1,956
Michael, M. D., Kulkarni, R. N., Postic, C., Previs, S. F., Shulman, G. I., Magnuson, M. A., & Kahn, C. R. (2000) · Molecular Cell · doi:10.1016/s1097-2765(05)00015-8 · Cited by 1,296
Kammoun, H. L., Chabanon, H., Hainault, I., Luquet, S., Magnan, C., Koike, T., Ferré, P., & Foufelle, F. (2009) · Journal of Clinical Investigation · doi:10.1172/jci37007 · Cited by 732
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Kersten, S. (2001) · The EMBO Reports · doi:10.1093/embo-reports/kve071 · Cited by 700
Liang, G., Yang, J., Horton, J. D., Hammer, R. E., Goldstein, J. L., & Brown, M. S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m111421200 · Cited by 669
Osborne, T. F. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.r000017200 · Cited by 521
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Saltiel, A. R. (2021) · Journal of Clinical Investigation · doi:10.1172/jci142241 · Cited by 446
Miyazaki, M., Flowers, M. T., Sampath, H., Chu, K., Otzelberger, C., Liu, X., & Ntambi, J. M. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.10.014 · Cited by 414
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Iynedjian, P. B. (2009) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-008-8322-9 · Cited by 365
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Li, J., Thorne, L. N., Punjabi, N. M., Sun, C.-K., Schwartz, A. R., Smith, P. L., Marino, R. L., Rodriguez, A., Hubbard, W. C., O’Donnell, C. P., et al. (2005) · Circulation Research · doi:10.1161/01.res.0000183879.60089.a9 · Cited by 335
Kabir, M., Catalano, K. J., Ananthnarayan, S., Kim, S. P., Van Citters, G. W., Dea, M. K., & Bergman, R. N. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00203.2004 · Cited by 327
Dentin, R. (2005) · Journal of Clinical Investigation · doi:10.1172/jci25256 · Cited by 315
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Seo, J. B., Moon, H. M., Kim, W. S., Lee, Y. S., Jeong, H. W., Yoo, E. J., Ham, J., Kang, H., Park, M.-G., Steffensen, K. R., et al. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.8.3430-3444.2004 · Cited by 250
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Amemiya-Kudo, M., Shimano, H., Yoshikawa, T., Yahagi, N., Hasty, A. H., Okazaki, H., Tamura, Y., Shionoiri, F., Iizuka, Y., Ohashi, K., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m005353200 · Cited by 226
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Ramírez, C. M., Goedeke, L., Rotllan, N., Yoon, J.-H., Cirera-Salinas, D., Mattison, J. A., Suárez, Y., de Cabo, R., Gorospe, M., & Fernández-Hernando, C. (2013) · Molecular and Cellular Biology · doi:10.1128/mcb.00016-13 · Cited by 154
Hirano, Y., Yoshida, M., Shimizu, M., & Sato, R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m105200200 · Cited by 151
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Bideyan, L., Nagari, R., & Tontonoz, P. (2021) · Genes & Development · doi:10.1101/gad.348340.121 · Cited by 126
Lee, G., Kim, Y. Y., Jang, H., Han, J. S., Nahmgoong, H., Park, Y. J., Han, S. M., Cho, C., Lim, S., Noh, J.-R., et al. (2022) · Cell Metabolism · doi:10.1016/j.cmet.2022.03.010 · Cited by 111
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Kim, S.-Y., Kim, H.-I., Kim, T.-H., Im, S.-S., Park, S.-K., Lee, I.-K., Kim, K.-S., & Ahn, Y.-H. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m313223200 · Cited by 108
Sánchez, J., Palou, A., & Picó, C. (2009) · Endocrinology · doi:10.1210/en.2009-0466 · Cited by 98
Yang, M., Liu, W., Pellicane, C., Sahyoun, C., Joseph, B. K., Gallo-Ebert, C., Donigan, M., Pandya, D., Giordano, C., Bata, A., et al. (2014) · Journal of Lipid Research · doi:10.1194/jlr.m041335 · Cited by 93
Huang, Q., Ma, C., Chen, L., Luo, D., Chen, R., & Liang, F. (2018) · Frontiers in Endocrinology · doi:10.3389/fendo.2018.00370 · Cited by 78
Warrier, M., Hinds, T. D., Ledford, K. J., Cash, H. A., Patel, P. R., Bowman, T. A., Stechschulte, L. A., Yong, W., Shou, W., Najjar, S. M., et al. (2010) · Endocrinology · doi:10.1210/en.2009-1158 · Cited by 57
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Metadata
- Authors
- Foretz, M., Pacot, C., Dugail, I., Lemarchand, P., Guichard, C., le Lièpvre, X., Berthelier-Lubrano, C., Spiegelman, B., Kim, J. B., Ferré, P., & Foufelle, F.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1999-05-01
- Volume
- 19
- Issue
- 5
- Pages
- 3760-3768
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2158465207
- Cited by
- 548
- References
- 0
- FWCI
- 18.95
- Type
- journal article
- Language
- en
- Open access
- green
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