Effects of Lactobacillus gasseri OLL2809 and α-lactalbumin on university-student athletes: a randomized, double-blind, placebo-controlled clinical trial
Abstract
Strenuous exercise reduces immune cell function and increases the risk of respiratory and gastrointestinal infections. In addition, it affects mood state and causes physical fatigue. Athletes require both mental and physical conditioning to execute good performance. In this study, we conducted a randomized, double-blind, placebo-controlled clinical trial to evaluate the immunopotentiation and fatigue-alleviation effects of Lactobacillus gasseri OLL2809 (LG2809) and α-lactalbumin (αLA) in university-student athletes after strenuous exercise. A total of 44 university students who performed strenuous exercise daily were separated into 3 groups to receive a 4-week course of placebo, 100 mg LG2809, or 100 mg LG2809 in combination with 900 mg αLA, respectively. Before and after each dietary treatment, the subjects performed strenuous cycle ergometer exercise for 1 h. Before and after each exercise session, blood samples and visual analogue scale scores for fatigue were obtained. In addition, the mood of each subject before and after the dietary treatment was evaluated using the Profile of Mood States (POMS) questionnaire. LG2809 ingestion was effective in preventing reduced natural killer cell activity due to strenuous exercise and elevating mood from a depressed state. In addition, LG2809 + αLA was found to alleviate minor resting fatigue, which was supported objectively by the significant reduction in the serum reactive oxygen metabolites and transforming growth factor β1 levels. These effects could be helpful for athletes to maintain mental and physical condition.
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Four weeks of supplementation with Lactobacillus gasseri OLL2809 and alpha-lactalbumin in 44 university-student athletes did not improve cycle ergometer performance.
“Four weeks of supplementation with Lactobacillus gasseri OLL2809 and alpha-lactalbumin in 44 university-student athletes did not improve cycle ergometer performance [67].”
From International Society of Sports Nutrition Position Stand: Probiotics (2019) · cites this paper as [67]
The single strain interventions used in five studies did not produce an aerobic performance benefit.
“The single strain interventions used in these five studies did not produce an aerobic performance benefit.”
From International Society of Sports Nutrition Position Stand: Probiotics (2019) · cites this paper as [67]
L. gasseri OLL2809 supplementation for 4 weeks prevented reduced NK cell activity after strenuous exercise in 44 university-student athletes.
“The probiotic supplementation prevented reduced NK cell activity after strenuous exercise which may enhance resistance against infections.”
From International Society of Sports Nutrition Position Stand: Probiotics (2019) · cites this paper as [67]
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Hanaoka, Y., Shimizu, K., Washiya, K., Tamura, A., Takemura, M., Furukawa, T., Miyamoto, T., Watanabe, K., & Akama, T. (2015) · 体力科學 · doi:10.7600/jspfsm.64.315 · Cited by 2 · see citing works
SPORCULARDA PROBİYOTİK TAKVİYESİ VE MİKROBİYATANIN PERFORMANSA ETKİSİ
Bozdoğan, F. B. K., & Tazeoğlu, A. (2022) · Spor ve Performans Araştırmaları Dergisi · doi:10.17155/omuspd.1077375 · Cited by 1 · see citing works
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THE INFLUENCE OF GUT MICROBIOTA ON PERFORMANCE IN ATHLETES
Barszcz, G., Bębenek, N., Błądek, M., Drygała, K., Wit, S., Frączkiewicz, P., Harpula, I., Ramotowski, R., Bogdan, J., Baljon, B., et al. (2025) · International Journal of Innovative Technologies in Social Science · doi:10.31435/ijitss.4(48).2025.4463
Ahmed, A. M. F., Akhiljith, Balasubramanian, S., Afeeza, M., Nair, M., George, J., & Saraswathy, G. R. (2026) · Clinical Nutrition ESPEN · doi:10.1016/j.clnesp.2026.105119
- Authors
- Sashihara, T., Nagata, M., Mori, T., Ikegami, S., Gotoh, M., Okubo, K., Uchida, M., & Itoh, H.
- Affiliations
- Meiji (Japan) (JP)
- Nippon Medical School Hospital (JP)
- Venue
- Applied Physiology, Nutrition, and Metabolism
- Publisher
- Canadian Science Publishing
- Published
- 2013-12
- Volume
- 38
- Issue
- 12
- Pages
- 1228-1235
- ISSN
- 1715-5312 (ISSN-L)
- 1715-5320
- OpenAlex
- W2014515358
- Cited by
- 46
- References
- 61
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 6 · 2022: 6 · 2021: 3 · 2020: 6 · 2019: 8 · 2018: 4 · 2017: 1 · 2016: 3 · 2015: 2
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Applied Physiology, Nutrition, and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- alpha-lactalbumin, strenuous exercise, athletes, fatigue, mood, natural killer cells, immune function, reactive oxygen species, TGF-β1, probiotics, dietary supplementation, exercise-induced immunosuppression
- MeSH terms
- Double-Blind Method, Humans, Lactalbumin, Lactobacillus, Students, Universities, Exercise, Athletes
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