The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Lipid Droplet Biogenesis
Open accessAbstract
Lipid droplets (LDs) are ubiquitous organelles that store neutral lipids for energy or membrane synthesis and act as hubs for metabolic processes. Cells generate LDs de novo, converting cells to emulsions with LDs constituting the dispersed oil phase in the aqueous cytoplasm. Here we review our current view of LD biogenesis. We present a model of LD formation from the ER in distinct steps and highlight the biology of proteins that govern this biophysical process. Areas of incomplete knowledge are identified, as are connections with physiology and diseases linked to alterations in LD biology.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Thematic review series: Glycerolipids. DGAT enzymes and triacylglycerol biosynthesis
Yen, C.-L. E., Stone, S. J., Koliwad, S., Harris, C., & Farese, R. V. (2008) · Journal of Lipid Research · doi:10.1194/jlr.r800018-jlr200 · Cited by 1,105
Self-citationCloning of DGAT2, a Second Mammalian Diacylglycerol Acyltransferase, and Related Family Members
Cases, S., Stone, S. J., Zhou, P., Yen, E., Tow, B., Lardizabal, K. D., Voelker, T., & Farese, R. V. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m106219200 · Cited by 818
Self-citationStone, S. J., Levin, M. C., Zhou, P., Han, J., Walther, T. C., & Farese, R. V. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m805768200 · Cited by 390
Self-citationPnpla3I148M knockin mice accumulate PNPLA3 on lipid droplets and develop hepatic steatosis
Smagris, E., BasuRay, S., Li, J., Huang, Y., Lai, K. V., Gromada, J., Cohen, J. C., & Hobbs, H. H. (2015) · Hepatology · doi:10.1002/hep.27242 · Cited by 379
DGAT2 Is a New Diacylglycerol Acyltransferase Gene Family
Lardizabal, K. D., Mai, J. T., Wagner, N. W., Wyrick, A., Voelker, T., & Hawkins, D. J. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m106168200 · Cited by 371
Evolutionarily conserved gene family important for fat storage
Kadereit, B., Kumar, P., Wang, W.-J., Miranda, D., Snapp, E. L., Severina, N., Torregroza, I., Evans, T., & Silver, D. L. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0708579105 · Cited by 252
Fat Storage-inducing Transmembrane Protein 2 Is Required for Normal Fat Storage in Adipose Tissue
Miranda, D. A., Kim, J.-H., Nguyen, L. N., Cheng, W., Tan, B. C., Goh, V. J., Tan, J. S. Y., Yaligar, J., KN, B. P., Velan, S. S., et al. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.547687 · Cited by 71
Cited by
Lipid droplet biogenesis and functions in health and disease
Zadoorian, A., Du, X., & Yang, H. (2023) · Nature Reviews Endocrinology · doi:10.1038/s41574-023-00845-0 · Cited by 617
Lipotoxicity and Diabetic Nephropathy: Novel Mechanistic Insights and Therapeutic Opportunities
Opazo-Ríos, L., Mas, S., Marín-Royo, G., Mezzano, S., Gómez-Guerrero, C., Moreno, J. A., & Egido, J. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21072632 · Cited by 363
Benador, I. Y., Veliova, M., Liesa, M., & Shirihai, O. S. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.02.011 · Cited by 363
APOE4 disrupts intracellular lipid homeostasis in human iPSC-derived glia
Sienski, G., Narayan, P., Bonner, J. M., Kory, N., Boland, S., Arczewska, A. A., Ralvenius, W. T., Akay, L., Lockshin, E., He, L., et al. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.aaz4564 · Cited by 336
Fanning, S., Haque, A., Imberdis, T., Baru, V., Barrasa, M. I., Nuber, S., Termine, D., Ramalingam, N., Ho, G. P. H., Noble, T., et al. (2019) · Molecular Cell · doi:10.1016/j.molcel.2018.11.028 · Cited by 285
Self-citationLipid droplets in the nervous system
Ralhan, I., Chang, C.-L., Lippincott-Schwartz, J., & Ioannou, M. S. (2021) · Journal of Cell Biology · doi:10.1083/jcb.202102136 · Cited by 281
Lipid droplets in neurodegenerative disorders
Farmer, B. C., Walsh, A. E., Kluemper, J. C., & Johnson, L. A. (2020) · Frontiers in Neuroscience · doi:10.3389/fnins.2020.00742 · Cited by 270
Chitraju, C., Walther, T. C., & Farese, R. V. (2019) · Journal of Lipid Research · doi:10.1194/jlr.m093112 · Cited by 206
Self-citationSpastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III
Chang, C.-L., Weigel, A. V., Ioannou, M. S., Pasolli, H. A., Xu, C. S., Peale, D. R., Shtengel, G., Freeman, M., Hess, H. F., Blackstone, C., et al. (2019) · Journal of Cell Biology · doi:10.1083/jcb.201902061 · Cited by 206
Lipid Droplets as Immune Modulators in Myeloid Cells
den Brok, M. H., Raaijmakers, T. K., Collado-Camps, E., & Adema, G. J. (2018) · Trends in Immunology · doi:10.1016/j.it.2018.01.012 · Cited by 203
TREM2-dependent lipid droplet biogenesis in phagocytes is required for remyelination
Gouna, G., Klose, C., Bosch-Queralt, M., Liu, L., Gokce, O., Schifferer, M., Cantuti-Castelvetri, L., & Simons, M. (2021) · Journal of Experimental Medicine · doi:10.1084/jem.20210227 · Cited by 186
Microglia and lipids: how metabolism controls brain innate immunity
Chausse, B., Kakimoto, P. A., & Kann, O. (2021) · Seminars in Cell & Developmental Biology · doi:10.1016/j.semcdb.2020.08.001 · Cited by 181
Lipid droplets and polyunsaturated fatty acid trafficking: Balancing life and death
Danielli, M., Perne, L., Jarc Jovičić, E., & Petan, T. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2023.1104725 · Cited by 180
Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma cells
Du, J., Su, Y., Qian, C., Yuan, D., Miao, K., Lee, D., Ng, A. H. C., Wijker, R. S., Ribas, A., Levine, R. D., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-18376-x · Cited by 171
Autophagy in hepatic adaptation to stress
Hazari, Y., Bravo-San Pedro, J. M., Hetz, C., Galluzzi, L., & Kroemer, G. (2020) · Journal of Hepatology · doi:10.1016/j.jhep.2019.08.026 · Cited by 151
Decoding microglial immunometabolism: a new frontier in Alzheimer's disease research
Jung, E. S., Choi, H., & Mook-Jung, I. (2025) · Molecular Neurodegeneration · doi:10.1186/s13024-025-00825-0 · Cited by 124
Mejhert, N., Kuruvilla, L., Gabriel, K. R., Elliott, S. D., Guie, M.-A., Wang, H., Lai, Z. W., Lane, E. A., Christiano, R., Danial, N. N., et al. (2020) · Molecular Cell · doi:10.1016/j.molcel.2020.01.014 · Cited by 121
Self-citationStructure and catalytic mechanism of a human triacylglycerol-synthesis enzyme
Sui, X., Wang, K., Gluchowski, N. L., Elliott, S. D., Liao, M., Walther, T. C., & Farese, R. V. (2020) · Nature · doi:10.1038/s41586-020-2289-6 · Cited by 120
Self-citationSegovia-Miranda, F., Morales-Navarrete, H., Kücken, M., Moser, V., Seifert, S., Repnik, U., Rost, F., Brosch, M., Hendricks, A., Hinz, S., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0660-7 · Cited by 109
Effects of TM6SF2 E167K on hepatic lipid and very low-density lipoprotein metabolism in humans
Borén, J., Adiels, M., Björnson, E., Matikainen, N., Söderlund, S., Rämö, J., Ståhlman, M., Ripatti, P., Ripatti, S., Palotie, A., et al. (2020) · JCI Insight · doi:10.1172/jci.insight.144079 · Cited by 81
Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation
Roberts, M. A., Deol, K. K., Mathiowetz, A. J., Lange, M., Leto, D. E., Stevenson, J., Hashemi, S. H., Morgens, D. W., Easter, E., Heydari, K., et al. (2023) · Developmental Cell · doi:10.1016/j.devcel.2023.07.001 · Cited by 81
Functional multi-organelle units control inflammatory lipid metabolism of macrophages
Zimmermann, J. A., Lucht, K., Stecher, M., Badhan, C., Glaser, K. M., Epple, M. W., Koch, L. R., Deboutte, W., Manke, T., Ebnet, K., et al. (2024) · Nature Cell Biology · doi:10.1038/s41556-024-01457-0 · Cited by 79
Selective Autophagy Receptors in Neuronal Health and Disease
Conway, O., Akpinar, H. A., Rogov, V. V., & Kirkin, V. (2020) · Journal of Molecular Biology · doi:10.1016/j.jmb.2019.10.013 · Cited by 75
Wang, C., Lu, J., Sha, X., Qiu, Y., Chen, H., & Yu, Z. (2023) · Experimental & Molecular Medicine · doi:10.1038/s12276-023-00935-z · Cited by 70
Roy, D., & Tedeschi, A. (2021) · Cells · doi:10.3390/cells10051078 · Cited by 68
Reducing microglial lipid load enhances β amyloid phagocytosis in an Alzheimer’s disease mouse model
Wu, X., Miller, J. A., Lee, B. T. K., Wang, Y., & Ruedl, C. (2025) · Science Advances · doi:10.1126/sciadv.adq6038 · Cited by 67
Effects of APOE4 allelic dosage on lipidomic signatures in the entorhinal cortex of aged mice
Miranda, A. M., Ashok, A., Chan, R. B., Zhou, B., Xu, Y., McIntire, L. B., Area-Gomez, E., Di Paolo, G., Duff, K. E., Oliveira, T. G., et al. (2022) · Translational Psychiatry · doi:10.1038/s41398-022-01881-6 · Cited by 60
MOSPD2 is an endoplasmic reticulum–lipid droplet tether functioning in LD homeostasis
Zouiouich, M., Di Mattia, T., Martinet, A., Eichler, J., Wendling, C., Tomishige, N., Grandgirard, E., Fuggetta, N., Fromental-Ramain, C., Mizzon, G., et al. (2022) · Journal of Cell Biology · doi:10.1083/jcb.202110044 · Cited by 40
Lipodystrophies—Disorders of the Fatty Tissue
Knebel, B., Müller-Wieland, D., & Kotzka, J. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21228778 · Cited by 39
A fluorogenic complementation tool kit for interrogating lipid droplet–organelle interaction
Li, X., Gamuyao, R., Wu, M.-L., Cho, W. J., King, S. V., Petersen, R. A., Stabley, D. R., Lindow, C., Climer, L. K., Shirinifard, A., et al. (2024) · Journal of Cell Biology · doi:10.1083/jcb.202311126 · Cited by 31
Casey, C. A., Donohue, T. M., Kubik, J. L., Kumar, V., Naldrett, M. J., Woods, N. T., Frisbie, C. P., McNiven, M. A., & Thomes, P. G. (2021) · Journal of Lipid Research · doi:10.1016/j.jlr.2021.100049 · Cited by 23
PI(3)P and DFCP1 regulate the biogenesis of lipid droplets
Lukmantara, I., Chen, F., Mak, H. Y., Zadoorian, A., Du, X., Xiao, F. N., Norris, D. M., Pandzic, E., Whan, R., Zhong, Q., et al. (2022) · Molecular Biology of the Cell · doi:10.1091/mbc.e22-07-0279 · Cited by 20
Tld1 is a regulator of triglyceride lipolysis that demarcates a lipid droplet subpopulation
Speer, N. O., Braun, R. J., Reynolds, E. G., Brudnicka, A., Swanson, J. M. J., & Henne, W. M. (2024) · Journal of Cell Biology · doi:10.1083/jcb.202303026 · Cited by 19
Revisiting the Pathogenesis of X-Linked Adrenoleukodystrophy
Bougnères, P., & Le Stunff, C. (2025) · Genes · doi:10.3390/genes16050590 · Cited by 11
Metadata
- Authors
- Walther, T. C., Chung, J., & Farese, R. V.
- Venue
- Annual Review of Cell and Developmental Biology
- Publisher
- Annual Reviews
- Published
- 2017-10-06
- Volume
- 33
- Issue
- 1
- Pages
- 491-510
- ISSN
- 1081-0706 (ISSN-L)
- 1530-8995
- OpenAlex
- W2743619083
- Cited by
- 762
- References
- 7
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
