Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes
Open accessAbstract
Type 2 diabetes mellitus is a complex metabolic disease, and cardiovascular disease is a leading complication of diabetes. Epicardial adipose tissue surrounding the heart displays biochemical, thermogenic, and cardioprotective properties. However, the metabolic cross-talk between epicardial fat and the myocardium is largely unknown. This study sought to understand epicardial adipose tissue metabolism from heart failure patients with or without diabetes. We aimed to unravel possible differences in glucose and lipid metabolism between human epicardial and subcutaneous adipocytes and elucidate the potential underlying mechanisms involved in heart failure. Insulin-stimulated [(14)C]glucose uptake and isoproterenol-stimulated lipolysis were measured in isolated epicardial and subcutaneous adipocytes. The expression of genes involved in glucose and lipid metabolism was analyzed by reverse transcription-polymerase chain reaction in adipocytes. In addition, epicardial and subcutaneous fatty acid composition was analyzed by high-resolution proton nuclear magnetic resonance spectroscopy. The difference between basal and insulin conditions in glucose uptake was significantly decreased (P= 0.006) in epicardial compared with subcutaneous adipocytes. Moreover, a significant (P< 0.001) decrease in the isoproterenol-stimulated lipolysis was also observed when the two fat depots were compared, and it was strongly correlated with lipolysis, lipid storage, and inflammation-related gene expression. Moreover, the fatty acid composition of these tissues was significantly altered by diabetes. These results emphasize potential metabolic differences between both fat depots in the presence of heart failure and highlight epicardial fat as a possible therapeutic target in situ in the cardiac microenvironment.
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EAT provides FAs directly to myocardium at times of high energy demand and implements local triacylglycerol storage as request decreases.
“To that end, EAT provides FAs directly to myocardium at times of high energy demand and implements the local triacylglycerol storage as request decreases [34,35].”
From Dysregulated epicardial adipose tissue as a risk factor and potential therapeutic target of heart failure with preserved ejection fraction in diabetes (2022) · cites this paper as [34]
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Research works printed but not linked: 3
- lucose uptake and lipid metabolism are impaired in epicardial adipose t... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824138/?report=printable 12 de 37 15-05-2019, 15:50
- Open bars, basal; gray bars, insulin. Glucose uptake and lipid metabolism are impaired in epicardial adipose t... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824138/?report=printable 26 de 37 15-05-2019, 15:50 1 Glucose uptake and lipid metabolism are impaired in epicardial adipose t... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824138/?report=printable 32 de 37 15-05-2019, 15:50
- adrenergic receptor (B1AR), and PKA]. Gene expression was normalized using the reference gene ACTB that was selected based on our previous results demonstrating that it does not change under these conditions. A: correlation between lipid storage and lipolysis gene expression. B: lipid storage and lipolysis gene expression based on disease status. C: lipid storage and lipolysis gene expression based on tissue type. Statistical analysis: correlation between lipolysis and lipid storage in A was evaluated using the Spearman correlation coefficient; n = 74, ρ(67) = 0.839, P < 0.001. Lipid storage group in B and C was compared between factors (disease status and tissue type) using a nonparametric 2-way ANOVA. P value is as indicated. Light gray bars, lipid storage; dark gray bars, lipolysis. Glucose uptake and lipid metabolism are impaired in epicardial adipose t... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824138/?report=printable 34 de 37 15-05-2019, 15:50
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- Authors
- Burgeiro, A., Fuhrmann, A., Cherian, S., Espinoza, D., Jarak, I., Carvalho, R. A., Loureiro, M., Patrício, M., Antunes, M., & Carvalho, E.
- Affiliations
- Centro de Neurociências e Biologia Celular (PT)
- Quest International University Perak (MY)
- University of Coimbra (PT)
- Unidade Local de Saúde de Coimbra (PT)
- Arkansas Children's Hospital (US)
- Corresponding author
- Carvalho, Rui A.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2016-04-01
- Volume
- 310
- Issue
- 7
- Pages
- E550-E564
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- PMC
- PMC4824138
- OpenAlex
- W2264833793
- Cited by
- 76
- References
- 95
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 11 · 2023: 4 · 2022: 9 · 2021: 3 · 2020: 6 · 2019: 5 · 2018: 8 · 2017: 14 · 2016: 3
- FWCI
- 8.26
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Funders
- SPD/GIFT award
- European Foundation for the Study of Diabetes (EFSD)
- Fundacao para a Ciencia e Tecnologia (FCT) (SFRH/BPD/26837/2006, PTDC/SAU-OSM/104124/2008, UID/NEU/04539/2013, EXCL/DTP-PIC/0069/2012)
- Foundation for the National Institutes of Health (FNIH) (NIH P30AG028718, NIH RO1 AG033761)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- epicardial adipose tissue, heart failure, type 2 diabetes, glucose uptake, insulin resistance, lipolysis, subcutaneous adipose tissue, fatty acid composition, adipocyte gene expression, inflammation, cardiac microenvironment
- MeSH terms
- Adipose Tissue, Adrenergic beta-Agonists, Aged, Blood Glucose, Carbon Radioisotopes, Diabetes Mellitus, Type 2, Fatty Acids, Female, Glucose, Heart Failure, Humans, Hypoglycemic Agents, Insulin, Isoproterenol, Lipolysis, Male, Middle Aged, Pericardium, Case-Control Studies, Adipocytes, Lipid Metabolism
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