Cited by 76Cardiovascular Disease and AdiposityAdipose Tissue and MetabolismAdipokines, Inflammation, and Metabolic Diseases

Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes

Open access
Burgeiro, Ana, Fuhrmann, Amelia, Cherian, Sam, Espinoza, Daniel, Jarak, Ivana, Carvalho, Rui A., Loureiro, Marisa, Patrício, Miguel +2 more·2016·American Journal of Physiology-Endocrinology and Metabolism

Abstract

Type 2 diabetes mellitus is a complex metabolic disease, and cardiovascular disease is a leading complication of diabetes. Epicardial adipose tissue surrounding the heart displays biochemical, thermogenic, and cardioprotective properties. However, the metabolic cross-talk between epicardial fat and the myocardium is largely unknown. This study sought to understand epicardial adipose tissue metabolism from heart failure patients with or without diabetes. We aimed to unravel possible differences in glucose and lipid metabolism between human epicardial and subcutaneous adipocytes and elucidate the potential underlying mechanisms involved in heart failure. Insulin-stimulated [(14)C]glucose uptake and isoproterenol-stimulated lipolysis were measured in isolated epicardial and subcutaneous adipocytes. The expression of genes involved in glucose and lipid metabolism was analyzed by reverse transcription-polymerase chain reaction in adipocytes. In addition, epicardial and subcutaneous fatty acid composition was analyzed by high-resolution proton nuclear magnetic resonance spectroscopy. The difference between basal and insulin conditions in glucose uptake was significantly decreased (P= 0.006) in epicardial compared with subcutaneous adipocytes. Moreover, a significant (P< 0.001) decrease in the isoproterenol-stimulated lipolysis was also observed when the two fat depots were compared, and it was strongly correlated with lipolysis, lipid storage, and inflammation-related gene expression. Moreover, the fatty acid composition of these tissues was significantly altered by diabetes. These results emphasize potential metabolic differences between both fat depots in the presence of heart failure and highlight epicardial fat as a possible therapeutic target in situ in the cardiac microenvironment.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1152/ajpendo.00384.2015

Cites

95 of 95 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 3

  1. lucose uptake and lipid metabolism are impaired in epicardial adipose t... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824138/?report=printable 12 de 37 15-05-2019, 15:50
  2. Open bars, basal; gray bars, insulin. Glucose uptake and lipid metabolism are impaired in epicardial adipose t... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824138/?report=printable 26 de 37 15-05-2019, 15:50 1 Glucose uptake and lipid metabolism are impaired in epicardial adipose t... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824138/?report=printable 32 de 37 15-05-2019, 15:50
  3. adrenergic receptor (B1AR), and PKA]. Gene expression was normalized using the reference gene ACTB that was selected based on our previous results demonstrating that it does not change under these conditions. A: correlation between lipid storage and lipolysis gene expression. B: lipid storage and lipolysis gene expression based on disease status. C: lipid storage and lipolysis gene expression based on tissue type. Statistical analysis: correlation between lipolysis and lipid storage in A was evaluated using the Spearman correlation coefficient; n = 74, ρ(67) = 0.839, P < 0.001. Lipid storage group in B and C was compared between factors (disease status and tissue type) using a nonparametric 2-way ANOVA. P value is as indicated. Light gray bars, lipid storage; dark gray bars, lipolysis. Glucose uptake and lipid metabolism are impaired in epicardial adipose t... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824138/?report=printable 34 de 37 15-05-2019, 15:50

Cited by

Sorted by citations

Authors
Burgeiro, A., Fuhrmann, A., Cherian, S., Espinoza, D., Jarak, I., Carvalho, R. A., Loureiro, M., Patrício, M., Antunes, M., & Carvalho, E.
Affiliations
  • Centro de Neurociências e Biologia Celular (PT)
  • Quest International University Perak (MY)
  • University of Coimbra (PT)
  • Unidade Local de Saúde de Coimbra (PT)
  • Arkansas Children's Hospital (US)
Corresponding author
Carvalho, Rui A.
Venue
American Journal of Physiology-Endocrinology and Metabolism
Published
2016-04-01
Volume
310
Issue
7
Pages
E550-E564
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
OpenAlex
W2264833793
Cited by
76
References
95
Citations by year
2026: 5 · 2025: 8 · 2024: 11 · 2023: 4 · 2022: 9 · 2021: 3 · 2020: 6 · 2019: 5 · 2018: 8 · 2017: 14 · 2016: 3
FWCI
8.26
Type
journal article
Language
en
Open access
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
Funders
  • SPD/GIFT award
  • European Foundation for the Study of Diabetes (EFSD)
  • Fundacao para a Ciencia e Tecnologia (FCT) (SFRH/BPD/26837/2006, PTDC/SAU-OSM/104124/2008, UID/NEU/04539/2013, EXCL/DTP-PIC/0069/2012)
  • Foundation for the National Institutes of Health (FNIH) (NIH P30AG028718, NIH RO1 AG033761)
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
epicardial adipose tissue, heart failure, type 2 diabetes, glucose uptake, insulin resistance, lipolysis, subcutaneous adipose tissue, fatty acid composition, adipocyte gene expression, inflammation, cardiac microenvironment
MeSH terms
Adipose Tissue, Adrenergic beta-Agonists, Aged, Blood Glucose, Carbon Radioisotopes, Diabetes Mellitus, Type 2, Fatty Acids, Female, Glucose, Heart Failure, Humans, Hypoglycemic Agents, Insulin, Isoproterenol, Lipolysis, Male, Middle Aged, Pericardium, Case-Control Studies, Adipocytes, Lipid Metabolism

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review