Cited by 227Gut microbiota and healthInflammatory Bowel DiseaseGastrointestinal motility and disorders

Ulcerative colitis and irritable bowel patients exhibit distinct abnormalities of the gut microbiota

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Noor, Samah O, Ridgway, Karyn, Scovell, Louise, Kemsley, E Katherine, Lund, Elizabeth K, Jamieson, Crawford, Johnson, Ian T, Narbad, Arjan·2010·BMC Gastroenterology

Abstract

Background: Previous studies suggest a link between gut microbiota and the development of ulcerative colitis (UC) and irritable bowel syndrome (IBS). Our aim was to investigate any quantitative differences in faecal bacterial compositions in UC and IBS patients compared to healthy controls, and to identify individual bacterial species that contribute to these differences. Methods: Faecal microbiota of 13 UC patients, 11 IBS patients and 22 healthy volunteers were analysed by PCR-Denaturing Gradient Gel Electrophoresis (DGGE) using universal and Bacteroides specific primers. The data obtained were normalized using in-house developed statistical method and interrogated by multivariate approaches. The differentiated bands were excised and identified by sequencing the V3 region of the 16S rRNA genes. Results: Band profiles revealed that number of predominant faecal bacteria were significantly different between UC, IBS and control group (p < 10-4). By assessing the mean band numbers in UC (37 ± 5) and IBS (39 ± 6), compared to the controls (45 ± 3), a significant decrease in bacterial species is suggested (p = 0.01). There were no significant differences between IBS and UC. Biodiversity of the bacterial species was significantly lower in UC (μ = 2.94, σ = 0.29) and IBS patients (μ = 2.90, σ = 0.38) than controls (μ = 3.25, σ = 0.16; p = 0.01). Moreover, similarity indices revealed greater biological variability of predominant bacteria in UC and IBS compared to the controls (median Dice coefficients 76.1% (IQR 70.9 - 83.1), 73.8% (IQR 67.0 - 77.5) and 82.9% (IQR 79.1 - 86.7) respectively). DNA sequencing of discriminating bands suggest that the presence of Bacteroides vulgatus, B. ovatus, B. uniformis, and Parabacteroides sp. in healthy volunteers distinguishes them from IBS and UC patients. DGGE profiles of Bacteroides species revealed a decrease of Bacteroides community in UC relative to IBS and controls. Conclusion: Molecular profiling of faecal bacteria revealed abnormalities of intestinal microbiota in UC and IBS patients, while different patterns of Bacteroides species loss in particular, were associated with UC and IBS.

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  • Bacteroides and Prevotella have been found to be increased in other colitis models or patients with IBD.

    “We also found higher relative abundances of the genera Bacteroides and Prevotella in mice with less developed mucus, bacteria that have been found to be increased also in other colitis models or patients with IBD 39, 43.”

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Authors
Noor, S. O., Ridgway, K., Scovell, L., Kemsley, E. K., Lund, E. K., Jamieson, C., Johnson, I. T., & Narbad, A.
Affiliations
  • Norwich Research Park (GB)
  • Norfolk and Norwich University Hospital (GB)
Corresponding author
Narbad, Arjan
Venue
BMC Gastroenterology
Published
2010-11-12
Volume
10
Issue
1
Pages
134-134
ISSN
1471-230X (ISSN-L)
OpenAlex
W2086580205
Cited by
227
References
56
Citations by year
2026: 4 · 2025: 7 · 2024: 12 · 2023: 15 · 2022: 10 · 2021: 12 · 2020: 17 · 2019: 22 · 2018: 16 · 2017: 6 · 2016: 15 · 2015: 27 · 2014: 23 · 2013: 15 · 2012: 15
FWCI
5.35
Type
journal article
Language
en
Open access
Locations
  • BMC Gastroenterology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • UEA Digital Repository (University of East Anglia) (submittedVersion)
Funders
Biotechnology and Biological Sciences Research Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
ulcerative colitis, irritable bowel syndrome, gut microbiota, Bacteroides, Bacteroides vulgatus, Bacteroides ovatus, Bacteroides uniformis, Parabacteroides, bacterial diversity, 16S rRNA gene sequencing, PCR-DGGE, inflammatory bowel disease, dysbiosis
MeSH terms
Adult, Bacteroides, Colitis, Ulcerative, Feces, Female, Humans, Male, Middle Aged, Multivariate Analysis, Case-Control Studies, Polymerase Chain Reaction, Gastrointestinal Tract, Irritable Bowel Syndrome, Young Adult, Denaturing Gradient Gel Electrophoresis, Molecular Typing

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