Cited by 80Helminth infection and controlParasitic Diseases Research and TreatmentMollusks and Parasites Studies

Cross-reactions of sera from dogs infected with Angiostrongylus vasorum in commercially available Dirofilaria immitis test kits

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Schnyder, Manuela, Deplazes, Peter·2012·Parasites & Vectors

Abstract

BACKGROUND: Dirofilaria immitis and Angiostrongylus vasorum are both important potentially fatal canine nematodes with overlapping endemic areas, especially in Europe. The preadult and adult stages of both species are living in the Arteria pulmonalis and the right heart, and diagnostically detectable circulating parasite antigens have been demonstrated for both species. For the detection of D. immitis infections, a variety of commercial tests have been developed, however, they have not been evaluated for cross-reactions against circulating antigens of A. vasorum. METHODS: In this study, potential cross-reactions of sera from 16 dogs, which were experimentally infected with A. vasorum and which had circulating antigens as confirmed by a species-specific ELISA, were evaluated for the detection of A. vasorum antigen in six commercially available D. immitis test kits. RESULTS: In three fast tests (Witness® Dirofilaria, SensPERT® Canine Heartworm, SNAP® 4Dx® Plus), all sera were negative. One fast membrane ELISA (SNAP® HTWM RT Test) was positive with four sera (25%), and one serum delivered a non-valid result twice. In the PetChek® HTWM PF Test, depending on the interpretation protocol, 5 or 8 dogs (31.2 - 50%) were positive. With the DiroCHEK®-ELISA, a single A. vasorum-infected dog (6.2%) tested positive. CONCLUSIONS: Due to potential cross-reactions with A. vasorum in commercially available test kits for the detection of D. immitis antigen, the simultaneous use of highly specific diagnostic methods for the differentiation of these two canine heart worms is recommended.

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  • The prevalence of D. immitis found in this study may be underestimated because the methods used may fail to identify some infected dogs, especially those with low D. immitis burdens.

    “Importantly, both methods used in this study to evaluate the prevalence of D. immitis may fail in the identification of some infected dogs, especially those with low D. immitis burdens and it is possible that the prevalence found in this study is underestimated.”

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  1. McCall JW Genchi C Kramer LH Guerrero J Venco L Heartworm disease in animals and humans Adv Parasitol 2008 66 193 285 18486691

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Authors
Schnyder, M., & Deplazes, P.
Affiliations
University of Zurich (CH)
Corresponding author
Schnyder, Manuela
Venue
Parasites & Vectors
Published
2012-11-13
Volume
5
Issue
1
Pages
258-258
ISSN
1756-3305 (ISSN-L)
OpenAlex
W2132293270
Cited by
80
References
44
Citations by year
2026: 5 · 2025: 3 · 2024: 2 · 2023: 5 · 2022: 11 · 2021: 4 · 2020: 11 · 2019: 5 · 2018: 6 · 2017: 9 · 2016: 5 · 2015: 9 · 2014: 4 · 2012: 1
FWCI
6.44
Type
journal article
Language
en
Open access
Locations
  • Parasites & Vectors (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Zurich Open Repository and Archive (University of Zurich) (acceptedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Zurich Open Repository and Archive (University of Zurich)
Topics
  • Health Sciences › Veterinary › Small Animals › Helminth infection and control
  • Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Mollusks and Parasites Studies
Keywords
Angiostrongylus vasorum, Dirofilaria immitis, canine heartworm disease, cross-reactivity, circulating antigens, dogs, test kit evaluation, ELISA, rapid diagnostic tests
MeSH terms
Angiostrongylus, Animals, Antigens, Helminth, Cross Reactions, Dirofilaria immitis, Dirofilariasis, Dog Diseases, Dogs, Immunoassay, Reagent Kits, Diagnostic, Sensitivity and Specificity, Strongylida Infections

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