Muscle Irisin Response to Aerobic vs HIIT in Overweight Female Adolescents
Open accessAbstract
BACKGROUND: Exercise stimulates the production of fibronectin type III domain-containing protein 5 (FNDC5), which is cleaved to release a protein called irisin. This protein induces browning of white adipose tissue resulting in increased thermogenesis. Different studies have measured circulating irisin at baseline and in response to exercise among a wide variety of individuals; yet, regarding the effect of different exercise intensities in obese adolescent girls, limited insight is available. This study compares the effect of acute aerobic exercise of moderate intensity and high-intensity interval training (HIIT) on irisin levels in skeletal muscle and plasma of sedentary overweight or obese female adolescents. METHODS: ), and two vastus lateralis muscle biopsies before and after session of workout. The session of aerobic exercise included cycling at 65% of their peak heart rate (HRpeak) for 40 min. In the HIIT group, exercise included six bouts of 1 min at 85-95% HRpeak separated by 1 min of recovery. Irisin levels were evaluated in samples of skeletal muscle (western blot) and plasma (ELISA). RESULTS: The levels of expression of irisin in skeletal muscle increased significantly after a session of HIIT (p < 0.05), while aerobic exercise no affect irisin levels. No significant differences between the groups in plasma irisin levels were found. CONCLUSIONS: The increase in muscle irisin levels was observed only following HIIT session. No increases in plasma irisin concentration were observed.
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Irisin secretion is preferentially upregulated in response to anaerobic stimuli.
“suggesting that irisin secretion is preferentially upregulated in response to anaerobic stimuli.”
From Exercise-Induced Muscle–Fat Crosstalk: Molecular Mediators and Their Pharmacological Modulation for the Maintenance of Metabolic Flexibility in Aging (2025) · cites this paper as [55]
The Results and Conclusions (no page given) report that a bout of HIIT increased irisin levels in skeletal muscle, while moderate aerobic exercise did not. They found no change in plasma irisin, however, so the study supports a preferential muscle-level response to HIIT, not preferential secretion in response to anaerobic stimuli generally. (LLM, conf=0.88)
Experimental — accuracy not yet measuredIn sedentary overweight or obese female adolescents, a session of anaerobic HIIT significantly increased skeletal muscle expression of irisin, whereas no such effect was observed following aerobic exercise.
“In sedentary overweight or obese female adolescents, a session of anaerobic high-intensity interval training (HIIT) significantly increased the skeletal muscle expression of irisin, whereas no such effect was observed following aerobic exercise [55]”
From Exercise-Induced Muscle–Fat Crosstalk: Molecular Mediators and Their Pharmacological Modulation for the Maintenance of Metabolic Flexibility in Aging (2025) · cites this paper as [55]
The Abstract Results report a significant increase in skeletal-muscle irisin after one HIIT session and no effect after aerobic exercise. The Methods > Subjects identify the participants as sedentary overweight or obese female adolescents. The Methods > Exercise session describe the HIIT protocol but do not establish that it was anaerobic, so that characterization is unsupported. (LLM, conf=0.84)
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- Authors
- Archundia-Herrera, C., Macias-Cervantes, M., Ruiz-Muñoz, B., Vargas-Ortiz, K., Kornhauser, C., & Perez-Vazquez, V.
- Affiliations
- Universidad de Guanajuato (MX)
- Venue
- Diabetology & Metabolic Syndrome
- Published
- 2017-12
- Volume
- 9
- Issue
- 1
- Pages
- 101-101
- ISSN
- 1758-5996 (ISSN-L)
- PMC
- PMC5746008
- OpenAlex
- W2778209067
- Cited by
- 72
- References
- 45
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 13 · 2023: 7 · 2022: 11 · 2021: 11 · 2020: 8 · 2019: 7 · 2018: 3
- FWCI
- 3.64
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Diabetology & Metabolic Syndrome (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Universidad de Guanajuato (MX) (DAIP/2013)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- irisin, FNDC5, high-intensity interval training, aerobic exercise, skeletal muscle, adipose tissue browning, thermogenesis, adolescent overweight, adolescent girls, exercise intensity, vastus lateralis
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