High-intensity exercise causes greater irisin response compared with low-intensity exercise under similar energy consumption
Open accessAbstract
Irisin is mainly released from skeletal muscle (myocytes) and promotes thermogenesis by browning of the white adipose tissue. Although exercise has been shown to increase irisin concentration in blood and myocytes via up-regulation peroxisome proliferator receptor γ coactivator-1α (PGC-1α) expression, the influence of exercise intensity on irisin secretion remains unclear. Therefore, we determined circulating irisin responses following a single bout of running at different intensities. Six sedentary males underwent treadmill running under two different conditions: a low-intensity (40% of VO2max) exercise trial (LIE) or a high-intensity (80% of VO2max) exercise trial (HIE). The exercises in LIE and HIE were lasted for 20 and 40 min, respectively. All subjects underwent the two trials on separate days, and a randomized cross-over design was used. Blood samples were collected before (Pre) and immediately after exercise, at 3, 6, and 19 h after exercise. Energy consumption during exercise did not significantly differ between the two trials. HIE significantly increased blood lactate and serum lactate dehydrogenase levels (P < 0.05). Compared with pre-exercise levels, the irisin concentrations were elevated at 6 h (18% increase) and 19 h (23% increase) after HIE, but significantly decreased after LIE. The relative irisin concentrations (compared with pre-exercise levels) were significantly greater in HIE than in LIE immediately after exercise, and at 6 and 19 h after exercise (P < 0.05). These findings suggest that irisin secretion after acute running exercise is affected by exercise intensity, independent of energy consumption.
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Training status can affect both baseline and exercise-induced myokine levels; individuals with metabolic syndrome exhibit attenuated irisin responses, and failure to distinguish acute and chronic measurement time points may have contributed to contradictory findings.
“Training status can affect both baseline and exercise-induced myokine levels. For instance, individuals with metabolic syndrome have been found to exhibit attenuated irisin responses [19], and failure to distinguish between acute and chronic measurement time points may have contributed to some contradictory findings in the literature [18,42,50,80].”
From Exercise-Induced Myokines in Obesity-Related Metabolic Disorders and Cardiovascular Protection: A Narrative Review (2026) · cites this paper as [18]
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- Authors
- Tsuchiya, Y., Ando, D., Goto, K., Kiuchi, M., Yamakita, M., & Koyama, K.
- Affiliations
- Ritsumeikan University (JP)
- National Defence Academy (IN)
- University of Yamanashi (JP)
- Yamanashi Prefectural University (JP)
- Venue
- The Tohoku Journal of Experimental Medicine
- Publisher
- Tohoku University Medical Press
- Published
- 2014
- Volume
- 233
- Issue
- 2
- Pages
- 135-140
- ISSN
- 0040-8727 (ISSN-L)
- 1349-3329
- OpenAlex
- W2086079683
- Cited by
- 176
- References
- 17
- Citations by year
- 2026: 9 · 2025: 14 · 2024: 11 · 2023: 16 · 2022: 23 · 2021: 25 · 2020: 19 · 2019: 13 · 2018: 14 · 2017: 13 · 2016: 6 · 2015: 10 · 2014: 3
- FWCI
- 10.35
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Tohoku Journal of Experimental Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (26282180)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- irisin, exercise intensity, high-intensity exercise, low-intensity exercise, treadmill running, skeletal muscle, PGC-1α, thermogenesis, white adipose tissue browning, blood lactate, lactate dehydrogenase, energy expenditure
- MeSH terms
- Energy Metabolism, Fibronectins, Gases, Humans, Male, Oxygen Consumption, Time Factors, Exercise, Young Adult
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