Cited by 117Thyroid Disorders and TreatmentsAdrenal Hormones and DisordersGrowth Hormone and Insulin-like Growth Factors

Induction of Type 3 Deiodinase Activity in Inflammatory Cells of Mice with Chronic Local Inflammation

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Boelen, A., Kwakkel, J., Alkemade, A., Renckens, R., Kaptein, E., Kuiper, G., Wiersinga, W. M., Visser, T. J.·2005·Endocrinology

Abstract

During illness, changes in thyroid hormone metabolism occur, so-called nonthyroidal illness (NTI). NTI has been characterized by a fall of serum T(3) due to decreased extrathyroidal conversion of T(4) into T(3) by liver type 1 deiodinase (D1), without an increase in serum TSH. Type 3 deiodinase (D3) was thought not to play an important role during NTI, but recently it has been shown that D3 activity is up-regulated in liver and skeletal muscle of critically ill patients related to hypoxia. We studied D3 gene expression and activity in liver and muscle/subcutis of mice during illness, which was induced by two different stimuli: bacterial endotoxin (lipopolysaccharide) administration, resulting in an acute systemic response, and a turpentine injection in each hindlimb, resulting in a local sc abscess. Lipopolysaccharide induced a rapid decrease in liver D1 and D3 activity but not skeletal muscle of hindlimb. In contrast, local inflammation induced by turpentine did not decrease liver D1 and D3 activity but increased markedly D3 activity in the muscle/subcutis sample containing the abscess, associated with strongly increased IL-1beta and IL-6 mRNA expression. Inflammatory cells, surrounding the abscess showed D3 and T(3)-transporter monocarboxylate transporter-8 immunoreactivity, whereas muscle cells did not show any immunoreactivity. In conclusion, local inflammation strongly induces D3 activity in inflammatory cells, especially in invading polymorphonuclear granulocytes, suggesting enhanced local degradation of T(3).

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  • DIO3 protein is highly expressed in infiltrating murine neutrophils during both bacterial infection and sterile inflammation.

    “DIO3 protein is highly expressed in infiltrating murine neutrophils during both bacterial infection and sterile inflammation [32, 33].”

    From An update on non-thyroidal illness syndrome (2021) · cites this paper as [32]

    The Turpentine-induced sterile abscess section reports D3 staining in inflammatory cells around a murine abscess, including polymorphonuclear granulocytes. It does not establish the same finding during bacterial infection: the LPS administration section reports low hindlimb muscle/subcutis D3 activity after bacterial-endotoxin treatment and does not report D3 staining in infiltrating neutrophils. (LLM, conf=0.90)

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  1. Wiersinga WM 2000 Nonthyroidal illness. In: Braverman LE, Utiger RD, eds. The thyroid. Philadelphia: Lippincott; 281-295

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Authors
Boelen, A., Kwakkel, J., Alkemade, A., Renckens, R., Kaptein, E., Kuiper, G., Wiersinga, W. M., & Visser, T. J.
Affiliations
  • Amsterdam UMC Location University of Amsterdam (NL)
  • Max Planck Institute for Metabolism Research (DE)
  • Erasmus MC (NL)
Corresponding author
Boelen, A.
Venue
Endocrinology
Published
2005-12
Volume
146
Issue
12
Pages
5128-5134
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
OpenAlex
W2036261582
Cited by
117
References
34
Citations by year
2026: 2 · 2025: 4 · 2024: 3 · 2023: 6 · 2022: 3 · 2021: 5 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 9 · 2016: 7 · 2015: 4 · 2014: 6 · 2013: 8 · 2012: 3
FWCI
4.55
Type
journal article
Language
en
Open access
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
  • Pure Amsterdam UMC (publishedVersion)
  • EUR Research Repository (Erasmus University Rotterdam) (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
  • RePub (Erasmus University Rotterdam) (submittedVersion, other-oa)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
Keywords
type 3 deiodinase, low T3 syndrome, thyroid hormone metabolism, local inflammation, lipopolysaccharide, type I deiodinase, type 2 deiodinase, IL-1β, interleukin-6, MCT8, polymorphonuclear leukocytes, skeletal muscle, liver, hypoxia
MeSH terms
Abscess, Animals, Chronic Disease, Female, Hindlimb, Immunohistochemistry, Inflammation, Injections, Intraperitoneal, Injections, Subcutaneous, Interleukin-1, Iodide Peroxidase, Irritants, Lipopolysaccharides, Liver, Mice, Inbred BALB C, Mice, Inbred C57BL, Muscular Diseases, RNA, Messenger, Turpentine, Interleukin-6, Muscle, Skeletal, Membrane Transport Proteins, Monocarboxylic Acid Transporters, Symporters, Subcutaneous Tissue, Mice

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