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Cited by 105Neuroendocrine regulation and behaviorGrowth Hormone and Insulin-like Growth FactorsNeuropeptides and Animal Physiology

Clearance Studies of Oxytocin in Humans Using Radioimmunoassay Measurements of the Hormone in Plasma and Urine*

AMICO, JANET A., ULBRECHT, JAN S., ROBINSON, ALAN G.·1987·The Journal of Clinical Endocrinology & Metabolism
DOI 10.1210/jcem-64-2-340
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Abstract

Synthetic oxytocin (OT) was infused iv in four men at 3 mU/min, and the rate was doubled every 90 min for a total of three infusion periods. The mean (+/- SEM) OT MCR was 16.4 +/- 1.7 ml/kg X min and was independent of the rate of infusion. A method for measuring OT in urine was developed using an octadecasilyl-silica column for extraction of the hormone. The extracted residue was reconstituted in potassium phosphate buffer, pH 7.4, for RIA. The minimum detectable level of OT in urine was 0.2 microU/ml (defined as a bound to free ratio of approximately 90%). The mean recovery of OT was 77 +/- 2%. The mean (+/- SEM) concentration of endogenous OT in urine was 10.2 +/- 1.4 microU/ml. Endogenous OT in urine eluted from a reverse phase high pressure liquid chromatography column as a single peak of OT immunoreactivity in the position of synthetic OT. Urinary OT excretion during infusion of synthetic OT was linearly correlated with plasma OT concentration whether calculated as microunits of urinary OT per mg creatinine (r = 0.89) or urinary OT per min (r = 0.93). Mean urinary fractional clearance of OT (OT clearance/creatinine clearance) was 3.6% renal clearance of OT (5.5 ml/min or 0.43% of MCR). Thus, OT MCR was constant over a wide range of physiological plasma OT levels and was similar to MCR in pregnant women studied previously in this laboratory. Less than 1% of OT was cleared in urine. This study defines the relationship between urinary and plasma OT during steady state infusion of physiological concentrations of the hormone and indicates that measurements of OT in urine by RIA may prove helpful for pharmacokinetic and physiological studies of OT-related events in humans.

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  • The clearance of oxytocin into urine in chimpanzees was taken to be 15 to 60 min after secretion, adapted from a human clearance study.

    “We took the clearance of oxytocin into urine in chimpanzees to be 15 to 60 min after secretion (19, 20), adapted from a human clearance study (43).”

    From Oxytocin reactivity during intergroup conflict in wild chimpanzees (2017) · cites this paper as [43]

  • Urine was collected 15-60 min after the target behaviour, the time window of urinary clearance of oxytocin for primates.

    “Specifically, urine was collected 15 -60 min after the target behaviour (time window of urinary clearance of oxytocin for primates [65]).”

    From Food sharing is linked to urinary oxytocin levels and bonding in related and unrelated wild chimpanzees (2014) · cites this paper as [65]

  • For humans and the common marmoset, radiolabeled studies indicate a time course from circulation into the urine as 30-60 min for peak levels to appear, and this window appears consistent across the primate order.

    “For humans and the common marmoset, radiolabeled studies have indicated a time course from circulation into the urine as 30–60 min for peak levels to appear (Amico et al., 1987; Seltzer and Ziegler, 2007). This indicates that when examining responses to an event, the desired collection time would be within this window and the window appears to be consistent across the primate order.”

    From Endogenous peripheral oxytocin measures can give insight into the dynamics of social relationships: a review (2014) · cites this paper as Amico et al., 1987

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Authors
AMICO, J. A., ULBRECHT, J. S., & ROBINSON, A. G.
Affiliations
University of Pittsburgh (US)
Venue
The Journal of Clinical Endocrinology & Metabolism
Publisher
The Endocrine Society
Published
1987-02
Volume
64
Issue
2
Pages
340-345
ISSN
  • 0021-972X (ISSN-L)
  • 1945-7197
DOI
10.1210/jcem-64-2-340
OpenAlex
W1969668906
Cited by
105
References
21
Citations by year
2026: 2 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 7 · 2021: 4 · 2020: 5 · 2019: 9 · 2018: 10 · 2017: 4 · 2016: 6 · 2015: 7 · 2014: 7 · 2013: 5 · 2012: 5
FWCI
2.03
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Pittsburgh
  • National Institutes of Health (5r01hd019787-03, 5r01am016166-12)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (1R01-HD-19787)
Topics
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
Keywords
oxytocin, renal clearance, endogenous oxytocin, pharmacokinetics
MeSH terms
Adult, Chromatography, High Pressure Liquid, Humans, Male, Mathematics, Metabolic Clearance Rate, Oxytocin, Radioimmunoassay

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