TGFβ2 knockout mice have multiple developmental defects that are non-overlapping with other TGFβ knockout phenotypes

Sanford, L. Philip; Ormsby, Ilona; Groot, Adriana C. Gittenberger-de; Sariola, Hannu; Friedman, Rick; Boivin, Gregory P.; Cardell, Emma Lou; Doetschman, Thomas

doi:10.1242/dev.124.13.2659

Cited by 1,447Developmental Biology and Gene RegulationTGF-β signaling in diseasesCraniofacial Disorders and Treatments

TGFβ2 knockout mice have multiple developmental defects that are non-overlapping with other TGFβ knockout phenotypes

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Sanford, L. Philip, Ormsby, Ilona, Groot, Adriana C. Gittenberger-de, Sariola, Hannu, Friedman, Rick, Boivin, Gregory P., Cardell, Emma Lou, Doetschman, Thomas·1997·Development

DOI 10.1242/dev.124.13.2659PMC PMC3850286

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Abstract

The growth and differentiation factor transforming growth factor-beta2 (TGFbeta2) is thought to play important roles in multiple developmental processes. Targeted disruption of the TGFbeta2 gene was undertaken to determine its essential role in vivo. TGFbeta2-null mice exhibit perinatal mortality and a wide range of developmental defects for a single gene disruption. These include cardiac, lung, craniofacial, limb, spinal column, eye, inner ear and urogenital defects. The developmental processes most commonly involved in the affected tissues include epithelial-mesenchymal interactions, cell growth, extracellular matrix production and tissue remodeling. In addition, many affected tissues have neural crest-derived components and simulate neural crest deficiencies. There is no phenotypic overlap with TGFbeta1- and TGFbeta3-null mice indicating numerous non-compensated functions between the TGFbeta isoforms.

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Two differentially expressed targets of MXI1, TGFB2 and NFIB, are involved in intellectual disability and craniofacial defects.
“Notably, two differentially expressed targets of MXI1, TGFB2 and NFIB, are also involved in intellectual disability and craniofacial defects (30, 36, 37).”
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Authors: Sanford, L. P., Ormsby, I., Groot, A. C. G.-D., Sariola, H., Friedman, R., Boivin, G. P., Cardell, E. L., & Doetschman, T.
Affiliations: University of Cincinnati (US)
Leiden University (NL)
University of Helsinki (FI)
Corresponding author: Doetschman, Thomas
ORCID: Groot, Adriana C. Gittenberger-de
Sariola, Hannu
Friedman, Rick
Venue: Development
Publisher: The Company of Biologists
Published: 1997-07-01
Volume: 124
Issue: 13
Pages: 2659-2670
ISSN: 0950-1991 (ISSN-L)
1477-9129
DOI: 10.1242/dev.124.13.2659
PMC: PMC3850286
OpenAlex: W2101314011
Cited by: 1,447
References: 76
Citations by year: 2026: 19 · 2025: 18 · 2024: 43 · 2023: 40 · 2022: 33 · 2021: 48 · 2020: 44 · 2019: 41 · 2018: 40 · 2017: 50 · 2016: 43 · 2015: 48 · 2014: 53 · 2013: 61 · 2012: 55
FWCI: 19.20
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: www.biologists.com/user-licence-1-1/ (vor)
Locations: Development (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Heart, Lung, and Blood Institute (P01HL041496, HL41496)
National Institute on Deafness and Other Communication Disorders (DC 00119)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 26471, R01HD026471)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Craniofacial Disorders and Treatments
Keywords: TGF-beta2, knockout mice, developmental defects, perinatal mortality, cardiac defects, lung development, craniofacial defects, limb malformations, eye defects, epithelial-mesenchymal interactions, extracellular matrix, tissue remodeling, neural crest, TGF-beta isoforms, compensatory function
MeSH terms: Abnormalities, Multiple, Animals, Bone and Bones, Cleft Palate, Cyanosis, Embryonic Induction, Epithelium, Eye Abnormalities, Genes, Homeobox, Heart Defects, Congenital, Ear, Inner, Mesoderm, Mice, Inbred C57BL, Phenotype, Tretinoin, Urogenital Abnormalities, Transforming Growth Factor beta, Mice, Knockout, Craniofacial Abnormalities, Mice

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TGFβ2 knockout mice have multiple developmental defects that are non-overlapping with other TGFβ knockout phenotypes

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