Preventive effect of dietary astaxanthin on UVA-induced skin photoaging in hairless mice
Open accessAbstract
Astaxanthin, a carotenoid found mainly in seafood, has potential clinical applications due to its antioxidant activity. In this study, we evaluated the effect of dietary astaxanthin derived from Haematococcus pluvialis on skin photoaging in UVA-irradiated hairless mice by assessing various parameters of photoaging. After chronic ultraviolet A (UVA) exposure, a significant increase in transepidermal water loss (TEWL) and wrinkle formation in the dorsal skin caused by UVA was observed, and dietary astaxanthin significantly suppressed these photoaging features. We found that the mRNA expression of lympho-epithelial Kazal-type-related inhibitor, steroid sulfatase, and aquaporin 3 in the epidermis was significantly increased by UVA irradiation for 70 days, and dietary astaxanthin significantly suppressed these increases in mRNA expression to be comparable to control levels. In the dermis, the mRNA expression of matrix metalloprotease 13 was increased by UVA irradiation and significantly suppressed by dietary astaxanthin. In addition, HPLC-PDA analysis confirmed that dietary astaxanthin reached not only the dermis but also the epidermis. Our results indicate that dietary astaxanthin accumulates in the skin and appears to prevent the effects of UVA irradiation on filaggrin metabolism and desquamation in the epidermis and the extracellular matrix in the dermis.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
AP-1 and NF-κB are associated with skin dryness, pigmentation, laxity, deep wrinkling, and apoptosis activation.
“These transcription factors are associated with skin dryness, pigmentation, laxity, deep wrinkling [55, 56], and apoptosis activation [57, 58].”
From Antioxidants from Plants Protect against Skin Photoaging (2018) · cites this paper as [56]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751 · see citing works
Ambati, R., Phang, S. M., Ravi, S., & Ravishankar, G. A. (2014) · Marine Drugs · doi:10.3390/md12010128 · Cited by 1,993 · see citing works
Haematococcus astaxanthin: applications for human health and nutrition
Guerin, M., Huntley, M. E., & Olaizola, M. (2003) · Trends in Biotechnology · doi:10.1016/s0167-7799(03)00078-7 · Cited by 1,495 · see citing works
Schweiger, D., Baufeld, C., Drescher, P., Oltrogge, B., Höpfner, S., Mess, A., Lüttke, J., Rippke, F., Filbry, A. W., & Max, H. (2013) · Skin Pharmacology and Physiology · doi:10.1159/000348473 · Cited by 1,348 · see citing works
Toxic effects of ultraviolet radiation on the skin
Matsumura, Y., & Ananthaswamy, H. N. (2004) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2003.08.019 · Cited by 1,270 · see citing works
Tanaka, M., Yamamoto, Y., Misawa, E., Nabeshima, K., Saito, M., Yamauchi, K., Abe, F., & Furukawa, F. (2016) · Skin Pharmacology and Physiology · doi:10.1159/000454718 · Cited by 1,092 · see citing works
Moisturization and skin barrier function
Rawlings, A. V., & Harding, C. R. (2004) · Dermatologic Therapy · doi:10.1111/j.1396-0296.2004.04s1005.x · Cited by 924 · see citing works
Filaggrin in the frontline: role in skin barrier function and disease
Sandilands, A., Sutherland, C. D., Irvine, A. D., & McLean, W. H. I. (2009) · Journal of Cell Science · doi:10.1242/jcs.033969 · Cited by 885 · see citing works
Epidermal Barrier Dysfunction in Atopic Dermatitis
Cork, M. J., Danby, S. G., Vasilopoulos, Y., Hadgraft, J., Lane, M. E., Moustafa, M., Guy, R. H., MacGowan, A., Ahnini, R. T., & Ward, S. J. (2009) · Journal of Investigative Dermatology · doi:10.1038/jid.2009.133 · Cited by 782 · see citing works
Slominski, A., Wortsman, J., Luger, T., Paus, R., & Solomon, S. (2000) · Physiological Reviews · doi:10.1152/physrev.2000.80.3.979 · Cited by 776 · see citing works
Matrix-Degrading Metalloproteinases in Photoaging
Quan, T., Qin, Z., Xia, W., Shao, Y., Voorhees, J. J., & Fisher, G. J. (2009) · Journal of Investigative Dermatology Symposium Proceedings · doi:10.1038/jidsymp.2009.8 · Cited by 740 · see citing works
Astaxanthin, a Carotenoid with Potential in Human Health and Nutrition
Hussein, G. M. E., Sankawa, U., Goto, H., Matsumoto, K., & Watanabe, H. (2005) · Journal of Natural Products · doi:10.1021/np050354+ · Cited by 653 · see citing works
Slominski, A. T., Zmijewski, M. A., Skobowiat, C., Zbytek, B., Slominski, R. M., & Steketee, J. D. (2012) · Advances in anatomy, embryology and cell biology · doi:10.1007/978-3-642-19683-6 · Cited by 502 · see citing works
Slominski (2012) · doi:10.1007/978-3-642-19683-6_1 · Cited by 461 · see citing works
Pashkow, F. J., Watumull, D. G., & Campbell, C. L. (2008) · The American Journal of Cardiology · doi:10.1016/j.amjcard.2008.02.010 · Cited by 446 · see citing works
A review of skin ageing and its medical therapy
GILCHREST, B. A. (1996) · British Journal of Dermatology · doi:10.1046/j.1365-2133.1996.d01-1088.x · Cited by 444 · see citing works
Key role of CRF in the skin stress response system
Slominski, A. T., Zmijewski, M. A., Zbytek, B., Tobin, D. J., Theoharides, T. C., & Rivier, J. (2013) · Endocrine Reviews · doi:10.1210/er.2012-1092 · Cited by 400 · see citing works
Impaired Stratum Corneum Hydration in Mice Lacking Epidermal Water Channel Aquaporin-3
Ma, T., Hara, M., Sougrat, R., Verbavatz, J., & Verkman, A. S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m200925200 · Cited by 274 · see citing works
Chew, B. P., Park, J. S., Wong, M. W., & Wong, T. S. (1999) · PubMed · Cited by 269 · see citing works
Cutaneous hypothalamic–pituitary–adrenal axis homolog: regulation by ultraviolet radiation
Skobowiat, C., Dowdy, J. C., Sayre, R. M., Tuckey, R. C., & Slominski, A. (2011) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00217.2011 · Cited by 269 · see citing works
Astaxanthin, cell membrane nutrient with diverse clinical benefits and anti-aging potential.
Kidd, P. M. (2011) · PubMed · Cited by 264 · see citing works
Caspase-14 reveals its secrets
Denecker, G., Ovaere, P., Vandenabeele, P., & Declercq, W. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200709098 · Cited by 252 · see citing works
Multifaceted pathways protect human skin from UV radiation
Natarajan, V. T., Ganju, P., Ramkumar, A., Grover, R., & Gokhale, R. S. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1548 · Cited by 244 · see citing works
Sugawara, T., Kushiro, M., Zhang, H., Ono, H., Nagao, A., & Nara, E. (2001) · Journal of Nutrition · doi:10.1093/jn/131.11.2921 · Cited by 240 · see citing works
Self-citationActivation of mammalian gene expression by the UV component of sunlight – from models to reality
Tyrrell, R. M. (1996) · BioEssays · doi:10.1002/bies.950180210 · Cited by 233 · see citing works
Potential Anti-Atherosclerotic Properties of Astaxanthin
Kishimoto, Y., Yoshida, H., & Kondo, K. N. (2016) · Marine Drugs · doi:10.3390/md14020035 · Cited by 222 · see citing works
Review of Animal Models in Carotenoid Research
Lee, C. M., Boileau, A. C., Boileau, T. W.-M., Williams, A. W., Swanson, K. S., Heintz, K. A., & Erdman, J. W. (1999) · Journal of Nutrition · doi:10.1093/jn/129.12.2271 · Cited by 200 · see citing works
On the role of skin in the regulation of local and systemic steroidogenic activities
Slominski, A. T., Manna, P. R., & Tuckey, R. C. (2015) · Steroids · doi:10.1016/j.steroids.2015.04.006 · Cited by 183 · see citing works
Ageing and photoageing of the skin: observations at the cellular and molecular level
Gilchrest, B. A., & Yaar, M. (1992) · British Journal of Dermatology · doi:10.1111/j.1365-2133.1992.tb16984.x · Cited by 171 · see citing works
Chemistry of carotenoid oxidation and free radical reactions
MARTIN, H. ‐., Ruck, C., Schmidt, M., Sell, S., Beutner, S., Mayer, B., & Walsh, R. (1999) · Pure and Applied Chemistry · doi:10.1351/pac199971122253 · Cited by 170 · see citing works
Melanocortin Receptor Ligands: New Horizons for Skin Biology and Clinical Dermatology
Böhm, M., Luger, T. A., Tobin, D. J., & García-Borrón, J. C. (2006) · Journal of Investigative Dermatology · doi:10.1038/sj.jid.5700421 · Cited by 165 · see citing works
Coral-Hinostroza, G., Ytrestøyl, T., Ruyter, B., & Bjerkeng, B. (2004) · Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology · doi:10.1016/j.cca.2004.09.011 · Cited by 152 · see citing works
Protective Effect of Fucoxanthin against UVB-Induced Skin Photoaging in Hairless Mice
URIKURA, I., Sugawara, T., & Hirata, T. (2011) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.110040 · Cited by 145 · see citing works
Self-citationUltraviolet radiation and skin cancer of humans
Urbach, F. (1997) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/s1011-1344(97)00029-8 · Cited by 136 · see citing works
Modulatory effects of an algal extract containing astaxanthin on UVA-irradiated cells in culture
Lyons, N. M., & O'Brien, N. M. (2002) · Journal of Dermatological Science · doi:10.1016/s0923-1811(02)00063-4 · Cited by 134 · see citing works
UVB activates hypothalamic-pituitary-adrenal axis in C57BL/6 mice
Skobowiat, C., & Slominski, A. T. (2015) · Journal of Investigative Dermatology · doi:10.1038/jid.2014.450 · Cited by 126 · see citing works
Increased expression of aquaporin 3 in atopic eczema
Olsson, M., Broberg, A., Jernås, M., Carlsson, L. M. S., Rudemo, M., Suurküla, M. B., Svensson, P., & Benson, M. (2006) · Allergy · doi:10.1111/j.1398-9995.2006.01151.x · Cited by 126 · see citing works
Role of cholesterol sulfate in epidermal structure and function: Lessons from X-linked ichthyosis
Elias, P. M., Williams, M. L. K., Choi, E. H., & Feingold, K. R. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2013.11.009 · Cited by 122 · see citing works
Upregulation of Aquaporin-3 Is Involved in Keratinocyte Proliferation and Epidermal Hyperplasia
Nakahigashi, K., Kabashima, K., Ikoma, A., Verkman, A. S., Miyachi, Y., & Hara‐Chikuma, M. (2010) · Journal of Investigative Dermatology · doi:10.1038/jid.2010.395 · Cited by 121 · see citing works
Kobayashi, M. (2000) · Applied Microbiology and Biotechnology · doi:10.1007/s002530000416 · Cited by 120 · see citing works
Skobowiat, C., Sayre, R. M., Dowdy, J. C., & Slominski, A. T. (2013) · British Journal of Dermatology · doi:10.1111/bjd.12096 · Cited by 115 · see citing works
Proteases: common culprits in human skin disorders
de Veer, S. J., Furio, L., Harris, J. M., & Hovnanian, A. (2014) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2013.11.005 · Cited by 107 · see citing works
Collagen Metabolism Is a Novel Target of the Neuropeptide α-Melanocyte-stimulating Hormone
Böhm, M., Raghunath, M., Sunderkötter, C. H., Schiller, M., Ständer, S., Brzoska, T., Cauvet, T., Schiöth, H. B., Schwarz, T., & Luger, T. A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m312549200 · Cited by 103 · see citing works
Breithaupt, D. E., Weller, P., Wolters, M., & Hahn, A. (2003) · British Journal Of Nutrition · doi:10.1079/bjn2003962 · Cited by 94 · see citing works
Jyonouchi, H., Sun, S., Tomita, Y., & Gross, M. D. (1995) · Journal of Nutrition · doi:10.1093/jn/125.10.2483 · Cited by 90 · see citing works
Skobowiat, C., Postlethwaite, A. E., & Slominski, A. T. (2016) · Photochemistry and Photobiology · doi:10.1111/php.12642 · Cited by 81 · see citing works
Sakabe, J., Yamamoto, M., Hirakawa, S., Motoyama, A., Ohta, I., Tatsuno, K., Ito, T., Kabashima, K., Hibino, T., & TOKURA, Y. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.476820 · Cited by 76 · see citing works
Tissue distribution of astaxanthin in rats following exposure to graded levels in the feed
Petri, D., & Lundebye, A. (2006) · Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology · doi:10.1016/j.cbpc.2006.12.008 · Cited by 73 · see citing works
The epidermal cholesterol sulfate cycle
Epstein, E. H., Williams, M. L. K., & Elias, P. M. (1984) · Journal of the American Academy of Dermatology · doi:10.1016/s0190-9622(84)80144-9 · Cited by 73 · see citing works
Loss of Collagen VII Is Associated with Reduced Transglutaminase 2 Abundance and Activity
Küttner, V., Mack, C., Gretzmeier, C., Bruckner‐Tuderman, L., & Dengjel, J. (2014) · Journal of Investigative Dermatology · doi:10.1038/jid.2014.185 · Cited by 59 · see citing works
Efficacy of Astaxanthin for the Treatment of Atopic Dermatitis in a Murine Model
Yoshihisa, Y., Andoh, T., Matsunaga, K., Rehman, M. U., Maoka, T., & Shimizu, T. (2016) · PLoS ONE · doi:10.1371/journal.pone.0152288 · Cited by 58 · see citing works
Lamore, S. D., & Wondrak, G. T. (2011) · Photochemical & Photobiological Sciences · doi:10.1039/c1pp05131h · Cited by 51 · see citing works
Bennett, K., Callard, R. E., Heywood, W., et al. (2010) · Journal of Proteome Research · doi:10.1021/pr1003467 · Cited by 50 · see citing works
Mariani, T. J., Sandefur, S. L., Roby, J. D., & Pierce, R. A. (1998) · Molecular Biology of the Cell · doi:10.1091/mbc.9.6.1411 · Cited by 49 · see citing works
Skobowiat, C., & Slominski, A. T. (2015) · Experimental Dermatology · doi:10.1111/exd.12890 · Cited by 39 · see citing works
Yamamoto, M., Miyai, M., Matsumoto, Y., Tsuboi, R., & Hibino, T. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.357467 · Cited by 37 · see citing works
Rapid, high‐quality and epidermal‐specific isolation of RNA from human skin
Trost, A., Bauer, J. W., Lanschuetzer, C. M., Laimer, M., Emberger, M., Hintner, H., & Önder, K. (2007) · Experimental Dermatology · doi:10.1111/j.1600-0625.2006.00534.x · Cited by 36 · see citing works
Miyazawa, T., Nakagawa, K., Kimura, F., et al. (2011) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.110368 · Cited by 34 · see citing works
An estimation of biological hazards due to solar radiation
Parisi, A. V., & Wong, J. C. F. (2000) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/s1011-1344(00)00006-3 · Cited by 32 · see citing works
Amelioration of ultraviolet-induced photokeratitis in mice treated with astaxanthin eye drops.
Lennikov, A., Kitaichi, N., Fukase, R., et al. (2012) · PubMed · Cited by 32 · see citing works
Shimada, E., Aida, K., Sugawara, T., & Hirata, T. (2011) · Journal of Oleo Science · doi:10.5650/jos.60.321 · Cited by 29 · see citing works
Self-citationUltraviolet radiation ( UVR ) activates central neuro‐endocrine‐immune system
Slominski, A. T. (2015) · Photodermatology Photoimmunology & Photomedicine · doi:10.1111/phpp.12165 · Cited by 26 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Astaxanthin in Skin Health, Repair, and Disease: A Comprehensive Review
Davinelli, S., Nielsen, M. E., & Scapagnini, G. (2018) · Nutrients · doi:10.3390/nu10040522 · Cited by 473 · see citing works
Antioxidants from Plants Protect against Skin Photoaging
Petruk, G., Del Giudice, R., Rigano, M. M., & Monti, D. M. (2018) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2018/1454936 · Cited by 283 · see citing works
Marine-Derived Compounds with Potential Use as Cosmeceuticals and Nutricosmetics
Alves, A., Sousa, E., Kijjoa, A., & Pinto, M. (2020) · Molecules · doi:10.3390/molecules25112536 · Cited by 169 · see citing works
Ito, N., Seki, S., & Ueda, F. (2018) · Nutrients · doi:10.3390/nu10070817 · Cited by 141 · see citing works
Do We Utilize Our Knowledge of the Skin Protective Effects of Carotenoids Enough?
Balić, A., & Mokos, M. (2019) · Antioxidants · doi:10.3390/antiox8080259 · Cited by 104 · see citing works
Effects of astaxanthin supplementation on skin health: A systematic review of clinical studies
Ng, Q. X., De Deyn, M. L. Z. Q., Loke, W., Foo, N. X., Chan, H. W., & Yeo, W. S. (2021) · Journal of Dietary Supplements · doi:10.1080/19390211.2020.1739187 · Cited by 57 · see citing works
Asada, K., Ohara, T., Muroyama, K., Yamamoto, Y., & Murosaki, S. (2019) · Journal of Cosmetic Dermatology · doi:10.1111/jocd.12890 · Cited by 32 · see citing works
Development of a whole-cell-based screening method for a carotenoid assay using aerial microalgae
Aburai, N., Kazama, H., Tsuruoka, A., Goto, M., & Abe, K. (2018) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.12.025 · Cited by 13 · see citing works
- Authors
- Komatsu, T., Sasaki, S., Manabe, Y., Hirata, T., & Sugawara, T.
- Affiliations
- Kyoto University (JP)
- Corresponding author
- Sugawara, Tatsuya
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2017-02-07
- Volume
- 12
- Issue
- 2
- Pages
- e0171178
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC5295690
- OpenAlex
- W2587629324
- Cited by
- 109
- References
- 62
- Citations by year
- 2026: 9 · 2025: 12 · 2024: 9 · 2023: 11 · 2022: 13 · 2021: 18 · 2020: 13 · 2019: 11 · 2018: 8 · 2017: 4
- FWCI
- 11.21
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Kyoto University Research Information Repository (Kyoto University) (submittedVersion, cc-by)
- Funders
- Fujifilm Corporation
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- astaxanthin, photoaging, UVA irradiation, Haematococcus pluvialis, dietary supplementation, transepidermal water loss, wrinkle formation, filaggrin, desquamation, AQP3, steroid sulfatase, matrix metalloproteinase-13, extracellular matrix, antioxidants, hairless mice
- MeSH terms
- Animals, Female, Mice, Hairless, RNA, Messenger, Skin, Ultraviolet Rays, Biomarkers, Skin Aging, Gene Expression, Dietary Supplements, Xanthophylls, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
