Acute and chronic effects of resistance training on skeletal muscle markers of mitochondrial remodeling in older adults
Open accessAbstract
We investigated the acute and chronic effects of resistance training (RT) on skeletal muscle markers of mitochondrial content and remodeling in older, untrained adults. Sixteen participants (n = 6 males, n = 10 females; age = 59 ± 4 years) completed 10 weeks of full-body RT (2 day/week). Muscle biopsies from the vastus lateralis were obtained prior to RT (Pre), 24 hr following the first training session (Acute), and 72 hr following the last training session (Chronic). Protein levels of mitochondrial electron transport chain complexes I-V (+39 to +180%, p ≤ .020) and markers of mitochondrial fusion Mfn1 (+90%, p = .003), Mfn2 (+110%, p < .001), and Opa1 (+261%, p = .004) increased following chronic RT. Drp1 protein levels also increased (+134%, p = .038), while Fis1 protein levels did not significantly change (-5%, p = .584) following chronic RT. Interestingly, protein markers of mitochondrial biogenesis (i.e., PGC-1α, TFAM, and NRF1) or mitophagy (i.e., Pink1 and Parkin) were not significantly altered (p > .050) after 10 weeks of RT. In summary, chronic RT promoted increases in content of electron transport chain proteins (i.e., increased protein levels of all five OXPHOS complexes) and increase in the levels of proteins related to mitochondrial dynamics (i.e., increase in fusion protein markers) in skeletal muscle of older adults. These results suggest that chronic RT could be a useful strategy to increase mitochondrial protein content in older individuals.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In that cohort, there was no change in PGC-1a and TFAM protein content, and activation of these signaling pathways was not interrogated.
“However, there was no change in PGC-1a and TFAM protein content, and the activation of these signaling pathways were not interrogated.”
From Skeletal Muscle Ribosome and Mitochondrial Biogenesis in Response to Different Exercise Training Modalities (2021) · cites this paper as Mesquita et al., 2020
The same cohort showed an increase in protein content of mitochondrial electron transport chain complexes and markers of mitochondrial remodeling.
“The same cohort of participants showed an increase in protein content of the mitochondrial electron transport chain complexes and markers of mitochondrial remodeling (Mesquita et al., 2020).”
From Skeletal Muscle Ribosome and Mitochondrial Biogenesis in Response to Different Exercise Training Modalities (2021) · cites this paper as Mesquita et al., 2020
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Wu, Z., Puigserver, P., Andersson, U., Zhang, C., Adelmant, G., Mootha, V., Troy, A., Cinti, S., Lowell, B., Scarpulla, R. C., et al. (1999) · Cell · doi:10.1016/s0092-8674(00)80611-x · Cited by 4,139 · see citing works
Mitochondrial Fission, Fusion, and Stress
Youle, R. J., & van der Bliek, A. M. (2012) · Science · doi:10.1126/science.1219855 · Cited by 3,674 · see citing works
Youle, R. J., & Narendra, D. P. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3028 · Cited by 3,273 · see citing works
Biochemical Adaptations in Muscle
Holloszy, J. O. (1967) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)96046-1 · Cited by 1,517 · see citing works
The mitochondrial basis of aging
Sun, N., Youle, R. J., & Finkel, T. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.01.028 · Cited by 1,441 · see citing works
Mitochondrial dynamics: overview of molecular mechanisms
Tilokani, L., Nagashima, S., Paupe, V., & Prudent, J. (2018) · Essays in Biochemistry · doi:10.1042/ebc20170104 · Cited by 1,397 · see citing works
Disruption of Fusion Results in Mitochondrial Heterogeneity and Dysfunction
Chen, H., Chomyn, A., & Chan, D. C. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m503062200 · Cited by 1,376 · see citing works
Folland, J. P., & Williams, A. G. (2007) · Sports Medicine · doi:10.2165/00007256-200737020-00004 · Cited by 1,347 · see citing works
Decline in skeletal muscle mitochondrial function with aging in humans
Short, K. R., Bigelow, M. L., Kahl, J., Singh, R., Coenen-Schimke, J., Raghavakaimal, S., & Nair, K. S. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501559102 · Cited by 1,333 · see citing works
Regulation of mitochondrial biogenesis
Jornayvaz, F. R., & Shulman, G. I. (2010) · Essays in Biochemistry · doi:10.1042/bse0470069 · Cited by 1,119 · see citing works
Handschin, C., & Spiegelman, B. M. (2006) · Endocrine Reviews · doi:10.1210/er.2006-0037 · Cited by 1,090 · see citing works
Mitofusin-2 Determines Mitochondrial Network Architecture and Mitochondrial Metabolism
Bach, D., Pich, S., Soriano, F. X., Vega, N., Baumgartner, B., Oriola, J., Daugaard, J. R., Lloberas, J., Camps, M., Zierath, J. R., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212754200 · Cited by 832 · cited 1× · see citing works
“Even though such observations regarding markers of mitochondrial remodeling are scarce, studies have also demonstrated that markers of mitochondrial fusion (e.g., Mfn1 and Mfn2) can directly impact mitochondrial function (Bach et al., 2003; Chen, Chomyn, & Chan, 2005).”
BackgroundYoon, Y., Krueger, E. W., Oswald, B. J., & McNiven, M. A. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.15.5409-5420.2003 · Cited by 820 · see citing works
Pernas, L. F., & Scorrano, L. (2015) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021115-105011 · Cited by 814 · see citing works
Mitochondrial dysfunction and sarcopenia of aging: from signaling pathways to clinical trials
Marzetti, E., Calvani, R., Cesari, M., Buford, T. W., Lorenzi, M., Behnke, B. J., & Leeuwenburgh, C. (2013) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2013.06.024 · Cited by 627 · see citing works
Robinson, M. M., Dasari, S., Konopka, A. R., Johnson, M. L., Manjunatha, S., Esponda, R. R., Carter, R. E., Lanza, I. R., & Nair, K. S. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.02.009 · Cited by 539 · see citing works
Transcriptomic profiling of skeletal muscle adaptations to exercise and inactivity
Pillon, N. J., Gabriel, B. M., Dollet, L., Smith, J. A. B., Sardón Puig, L., Botella, J., Bishop, D. J., Krook, A., & Zierath, J. R. (2020) · Nature Communications · doi:10.1038/s41467-019-13869-w · Cited by 457 · see citing works
The role of mitochondria in aging
Jang, J. Y., Blum, A., Liu, J., & Finkel, T. (2018) · Journal of Clinical Investigation · doi:10.1172/jci120842 · Cited by 358 · see citing works
Mitochondrial fusion supports increased oxidative phosphorylation during cell proliferation
Yao, C.-H., Wang, R., Wang, Y., Kung, C.-P., Weber, J. D., & Patti, G. J. (2019) · eLife · doi:10.7554/elife.41351 · Cited by 338 · see citing works
Visser, M., & Schaap, L. A. (2011) · Clinics in Geriatric Medicine · doi:10.1016/j.cger.2011.03.006 · Cited by 320 · see citing works
Resistance exercise training alters mitochondrial function in human skeletal muscle
PORTER, C., REIDY, P. T., BHATTARAI, N., SIDOSSIS, L. S., & RASMUSSEN, B. B. (2015) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0000000000000605 · Cited by 288 · see citing works
Molecular mechanisms for mitochondrial adaptation to exercise training in skeletal muscle
Drake, J. C., Wilson, R. J., & Yan, Z. (2016) · The FASEB Journal · doi:10.1096/fj.15-276337 · Cited by 263 · see citing works
Hood, D. A. (2009) · Applied Physiology, Nutrition, and Metabolism · doi:10.1139/h09-045 · Cited by 242 · see citing works
Large energetic adaptations of elderly muscle to resistance and endurance training
Jubrias, S. A., Esselman, P. C., Price, L. B., Cress, M. E., & Conley, K. E. (2001) · Journal of Applied Physiology · doi:10.1152/jappl.2001.90.5.1663 · Cited by 213 · see citing works
Parise, G., BROSE, A., & Tarnopolsky, M. G. (2004) · Experimental Gerontology · doi:10.1016/j.exger.2004.09.002 · Cited by 196 · see citing works
Mitochondria, Muscle Health, and Exercise with Advancing Age
Carter, H. N., Chen, C. C. W., & Hood, D. A. (2015) · Physiology · doi:10.1152/physiol.00039.2014 · Cited by 191 · see citing works
Konopka, A. R., Suer, M. K., Wolff, C. A., & Harber, M. P. (2014) · The Journals of Gerontology: Series A · doi:10.1093/gerona/glt107 · Cited by 187 · see citing works
Mitochondrial Dynamics in Basal and Stressful Conditions
Zemirli, N., Morel, É., & Molino, D. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19020564 · Cited by 186 · see citing works
Ordureau, A., Paulo, J. A., Zhang, W., Ahfeldt, T., Zhang, J., Cohn, E. F., Hou, Z., Heo, J., Rubin, L. L., Sidhu, S. S., et al. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.03.012 · Cited by 184 · see citing works
González‐Freire, M., Scalzo, P., D’Agostino, J., Moore, A. Z., Díaz‐Ruiz, A., Fabbri, E., Zane, A. C., Chen, B. H., Becker, K. G., Lehrmann, E., et al. (2018) · Aging Cell · doi:10.1111/acel.12725 · Cited by 183 · see citing works
Herbst, E. A. F., Paglialunga, S., Gerling, C., Whitfield, J., Mukai, K., Chabowski, A., Heigenhauser, G. J. F., Spriet, L. L., & Holloway, G. P. (2014) · The Journal of Physiology · doi:10.1113/jphysiol.2013.267336 · Cited by 179 · see citing works
Sarcopenia: prevalence, mechanisms, and functional consequences
Berger, M. J., & Doherty, T. J. (2010) · Interdisciplinary topics in gerontology and geriatrics · doi:10.1159/000319997 · Cited by 175 · see citing works
Resistance training, sarcopenia, and the mitochondrial theory of aging
Johnston, A. P. W., De Lisio, M., & Parise, G. (2008) · Applied Physiology Nutrition and Metabolism · doi:10.1139/h07-141 · Cited by 174 · see citing works
Mitochondria as a Target for Mitigating Sarcopenia
Coen, P. M., Musci, R., Hinkley, J., & Miller, B. F. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2018.01883 · Cited by 140 · see citing works
Combined Training Enhances Skeletal Muscle Mitochondrial Oxidative Capacity Independent of Age
Irving, B. A., Lanza, I. R., Henderson, G. C., Rao, R. R., Spiegelman, B. M., & Nair, K. S. (2015) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-3081 · Cited by 139 · see citing works
Holloway, G. P., Holwerda, A. M., Miotto, P. M., Dirks, M. L., Verdijk, L. B., & van Loon, L. J. C. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.02.069 · Cited by 134 · see citing works
Arribat, Y., Broskey, N. T., Greggio, C., Boutant, M., Conde Alonso, S., Kulkarni, S. S., Lagarrigue, S., Carnero, E. A., Besson, C., Cantó, C., et al. (2019) · Acta Physiologica · doi:10.1111/apha.13179 · Cited by 112 · see citing works
Sex differences in mitochondrial respiratory function in human skeletal muscle
Miotto, P. M., McGlory, C., Holloway, T. M., Phillips, S. M., & Holloway, G. P. (2018) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00025.2018 · Cited by 109 · see citing works
Balan, E., Schwalm, C., Naslain, D., Nielens, H., Francaux, M., & Deldicque, L. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.01088 · Cited by 104 · see citing works
Zhong, X., Cui, P., Cai, Y., Wang, L., He, X., Long, P., Lu, K., Yan, R., Zhang, Y., Pan, X., et al. (2018) · Cell Metabolism · doi:10.1016/j.cmet.2018.11.007 · Cited by 95 · see citing works
PINK1-dependent phosphorylation of PINK1 and Parkin is essential for mitochondrial quality control
Zhuang, N., Li, L., Chen, S., & Wang, T. (2016) · Cell Death and Disease · doi:10.1038/cddis.2016.396 · Cited by 92 · see citing works
Human Skeletal Muscle Mitochondrial Adaptations Following Resistance Exercise Training
Parry, H. A., Roberts, M. D., & Kavazis, A. N. (2020) · International Journal of Sports Medicine · doi:10.1055/a-1121-7851 · Cited by 92 · see citing works
Self-citationZampieri, S., Mammucari, C., Romanello, V., Barberi, L., Pietrangelo, L., Fusella, A., Mosole, S., Gherardi, G., Höfer, C., Löfler, S., et al. (2016) · Physiological Reports · doi:10.14814/phy2.13005 · Cited by 88 · see citing works
Resistance exercise–induced changes in muscle phenotype are load dependent
LIM, C., KIM, H. J., MORTON, R. W., HARRIS, R., PHILLIPS, S. M., JEONG, T. S., & KIM, C. K. (2019) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0000000000002088 · Cited by 81 · see citing works
Rescue of Pink1 Deficiency by Stress-Dependent Activation of Autophagy
Zhang, Y., Nguyen, D. T., Olzomer, E. M., Poon, G. P., Cole, N. J., Puvanendran, A., Phillips, B. R., & Hesselson, D. (2017) · Cell chemical biology · doi:10.1016/j.chembiol.2017.03.005 · Cited by 64 · see citing works
Campbell, G. R., To, R. K., & Spector, S. A. (2019) · mBio · doi:10.1128/mbio.02638-19 · Cited by 63 · see citing works
Islam, H., Hood, D. A., & Gurd, B. J. (2019) · Applied Physiology, Nutrition, and Metabolism · doi:10.1139/apnm-2019-0069 · Cited by 62 · see citing works
Lamb, D. A., Moore, J. H., Mesquita, P. H. C., Smith, M. A., Vann, C. G., Osburn, S. C., Fox, C. D., Lopez, H. L., Ziegenfuss, T. N., Huggins, K. W., et al. (2020) · Aging · doi:10.18632/aging.103218 · Cited by 62 · see citing works
Self-citationTarnopolsky, M. A. (2009) · Applied Physiology Nutrition and Metabolism · doi:10.1139/h09-022 · Cited by 59 · see citing works
Kitaoka, Y., Ogasawara, R., Tamura, Y., Fujita, S., & Hatta, H. (2015) · Applied Physiology Nutrition and Metabolism · doi:10.1139/apnm-2015-0184 · Cited by 54 · see citing works
Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1
Liu, X., Peyton, K. J., & Durante, W. (2013) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00872.2012 · Cited by 52 · see citing works
Ogborn, D. I., McKay, B. R., Crane, J. D., Safdar, A., Akhtar, M., Parise, G., & Tarnopolsky, M. A. (2015) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00005.2014 · Cited by 49 · see citing works
Nuclear respiratory factor-1 and bioenergetics in tamoxifen-resistant breast cancer cells
Radde, B. N., Ivanova, M. M., Mai, H. X., Alizadeh‐Rad, N., Piell, K. M., Hoose, P. M. V., Cole, M. P., Muluhngwi, P., Kalbfleisch, T., Rouchka, E. C., et al. (2016) · Experimental Cell Research · doi:10.1016/j.yexcr.2016.08.006 · Cited by 49 · see citing works
Miller, M. S., Callahan, D. M., Tourville, T. W., Slauterbeck, J. R., Kaplan, A., Fiske, B. R., Savage, P. D., Ades, P. A., Beynnon, B. D., & Toth, M. J. (2017) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00830.2016 · Cited by 44 · see citing works
Bovine Milk Extracellular Vesicles (EVs) Modification Elicits Skeletal Muscle Growth in Rats
Parry, H. A., Mobley, C. B., Mumford, P. W., Romero, M. A., Haun, C. T., Zhang, Y., Roberson, P. A., Zempleni, J., Ferrando, A. A., Vechetti, I. J., et al. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.00436 · Cited by 41 · see citing works
Self-citationAlvehus, M., Boman, N., Söderlund, K., Svensson, M., & Burén, J. (2014) · European Journal of Applied Physiology · doi:10.1007/s00421-014-2879-9 · Cited by 39 · see citing works
Resistance exercise training and in vitro skeletal muscle oxidative capacity in older adults
Flack, K. D., Davy, B. M., DeBerardinis, M., Boutagy, N. E., McMillan, R. P., Hulver, M. W., Frisard, M. I., Anderson, A. S., Savla, J. T., & Davy, K. P. (2016) · Physiological Reports · doi:10.14814/phy2.12849 · Cited by 34 · see citing works
Kephart, W. C., Mobley, C. B., Fox, C. D., Pascoe, D. D., Sefton, J. M., Wilson, T. J., Goodlett, M. D., Kavazis, A. N., Roberts, M. D., & Martin, J. S. (2015) · Experimental Physiology · doi:10.1113/ep085160 · Cited by 32 · see citing works
Self-citationSkeletal Muscle Mitochondrial Adaptations to Maximal Strength Training in Older Adults
Berg, O. K., Kwon, O. S., Hureau, T. J., Clifton, H. L., Thurston, T. S., Fur, Y. L., Jeong, E., Trinity, J. D., Richardson, R. S., Wang, E., et al. (2020) · The Journals of Gerontology Series A · doi:10.1093/gerona/glaa082 · Cited by 21 · see citing works
Mitochondrial Adaptations in Aged Skeletal Muscle: Effect of Exercise Training
Ziaaldini, M. M., Hosseini, S. R. A., & Fathi, M. (2017) · Physiological Research · doi:10.33549/physiolres.933329 · Cited by 16 · see citing works
Mitochondrial basis for sex-differences in metabolism and exercise performance
Ferreira, L. F. (2018) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00077.2018 · Cited by 14 · see citing works
Induced in vivo knockdown of the Brca1 gene in skeletal muscle results in skeletal muscle weakness
Tarpey, M. D., Valencia, A. P., Jackson, K. C., Amorese, A. J., Balestrieri, N. P., Renegar, R. H., Pratt, S. J. P., RYAN, T. E., McClung, J. M., Lovering, R. M., et al. (2018) · The Journal of Physiology · doi:10.1113/jp276863 · Cited by 12 · see citing works
Postmenopausal Effects of Resistance Training on Muscle Damage and Mitochondria
Manfredi, T. G., Monteiro, M. A., Lamont, L. S., et al. (2012) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0b013e318277a1e4 · Cited by 10 · see citing works
Islam, H., & Bonafiglia, J. T. (2019) · The Journal of Physiology · doi:10.1113/jp278538 · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Mitophagy in human health, ageing and disease
Picca, A., Faitg, J., Auwerx, J., Ferrucci, L., & D’Amico, D. (2023) · Nature Metabolism · doi:10.1038/s42255-023-00930-8 · Cited by 635 · see citing works
Mesquita, P. H. C., Vann, C. G., Phillips, S. M., McKendry, J., Young, K. C., Kavazis, A. N., & Roberts, M. D. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.725866 · Cited by 62 · see citing works
Self-citation
- Authors
- Mesquita, P. H. C., Lamb, D. A., Parry, H. A., Moore, J. H., Smith, M. A., Vann, C. G., Osburn, S. C., Fox, C. D., Ruple, B. A., Huggins, K. W., Fruge, A. D., Young, K. C., Kavazis, A. N., & Roberts, M. D.
- Affiliations
- Auburn University (US)
- Department of Nutrition, Dietetics and Hospitality Management Auburn University Auburn AL USA
- Corresponding author
- Roberts, Michael D.
- ORCID
- Venue
- Physiological Reports
- Publisher
- Wiley
- Published
- 2020-08
- Volume
- 8
- Issue
- 15
- Pages
- e14526-e14526
- ISSN
- 2051-817X (ISSN-L)
- PMC
- PMC7399374
- OpenAlex
- W3047221068
- Cited by
- 66
- References
- 64
- Citations by year
- 2026: 13 · 2025: 8 · 2024: 14 · 2023: 13 · 2022: 11 · 2021: 5 · 2020: 2
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Physiological Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Peanut Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- resistance training, mitochondrial remodeling, skeletal muscle, older adults, mitochondrial fusion, mitochondrial fission, respiratory chain complexes, oxidative phosphorylation, Mfn1, mitofusin 2, OPA1, Drp1, PGC-1α, mitophagy, vastus lateralis
- MeSH terms
- Aged, Female, Humans, Male, Membrane Proteins, Middle Aged, Mitochondria, Muscle, Oxidative Phosphorylation, Muscle, Skeletal, GTP Phosphohydrolases, Mitochondrial Proteins, Mitochondrial Membrane Transport Proteins, Resistance Training, Mitochondrial Dynamics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
