Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy
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INTRODUCTION: Current gene therapy involves replacement of defective gene by delivery of healthy genetic material to precede normal function. Virus-mediated gene delivery is the most successful and efficient method for gene therapy, but it has been challenged due to serious safety concerns. Conversely, gene delivery using plasmid DNA (pDNA) is considered safer, but its transfection efficiency is much lower than virus-mediated gene transfer. Recently, mRNA has been suggested as an alternative option to avoid undesired insertion of delivered DNA sequences with higher transfection efficiency and stability. AREA COVERED: In this review, we summarize the currently available strategies of mRNA modification to increase the therapeutic efficacy; we also highlight the recent improvements of mRNA delivery for in vivo applications of gene therapy. EXPERT OPINION: The use of mRNA-based gene transfer could indeed be a promising new strategy for gene therapy. Notable advantages include no risk of integration into the genomic DNA, adjustable gene expression and easier modulation of the immune system. By reducing or utilizing the immunogenic properties, mRNA offers a promising tool for gene/or transcript replacement.
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A non-replicating mRNA contains an open reading frame (ORF) encoding the gene coding for the target antigen flanked by 5′ and 3′ UTR; the 5′ end contains a 7-methylguanosine cap structure, whereas the 3′ end contains a poly(A) tail structure.
“A non-replicating mRNA contains an open reading frame (ORF) encoding the gene coding for the target antigen flanked by 5′ and 3′ UTR. The 5′ end contains a 7-methylguanosine cap structure (5′-cap, m7G), whereas the 3′ end contains a poly(A) tail structure.”
From Advances in COVID-19 mRNA vaccine development (2022) · cites this paper as [114]
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Cites
Sharp, P. M., & Li, W.-H. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.3.1281 · Cited by 3,992 · see citing works
Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA
Diebold, S. S., Kaisho, T., Hemmi, H., Akira, S., & Reis e Sousa, C. (2004) · Science · doi:10.1126/science.1093616 · Cited by 3,444 · see citing works
Karikó, K., Buckstein, M., Ni, H., & Weissman, D. (2005) · Immunity · doi:10.1016/j.immuni.2005.06.008 · Cited by 2,728 · see citing works
eIF4 Initiation Factors: Effectors of mRNA Recruitment to Ribosomes and Regulators of Translation
Gingras, A., Raught, B., & Sonenberg, N. (1999) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.68.1.913 · Cited by 2,103 · see citing works
Caput, D., Beutler, B., Hartog, K., Thayer, R. M., Brown-Shimer, S. L., & Cerami, A. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.6.1670 · Cited by 1,516 · see citing works
P bodies and the control of mRNA translation and degradation
Parker, R., & Sheth, U. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.02.011 · Cited by 1,335 · see citing works
The highways and byways of mRNA decay
Garneau, N. L., Wilusz, J., & Wilusz, C. J. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2104 · Cited by 1,294 · see citing works
Regulating the fate of mRNA: the control of cellular iron metabolism
Klausner, R. D., Rouault, T. A., & Harford, J. B. (1993) · Cell · doi:10.1016/0092-8674(93)90046-s · Cited by 1,210 · see citing works
Dendritic cells as therapeutic vaccines against cancer
Banchereau, J., & Palucka, A. K. (2005) · Nature reviews. Immunology · doi:10.1038/nri1592 · Cited by 1,195 · see citing works
mRNA is an endogenous ligand for toll-like receptor 3
Karikó, K., Ni, H., Capodici, J., Lamphier, M., & Weissman, D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310175200 · Cited by 1,062 · see citing works
Gene Therapy Progress and Prospects: Nonviral vectors
Niidome, T., & Huang, L. (2002) · Gene Therapy · doi:10.1038/sj.gt.3301923 · Cited by 967 · see citing works
Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo.
Boczkowski, D., Nair, S. K., Snyder, D., & Gilboa, E. (1996) · The Journal of experimental medicine · doi:10.1084/jem.184.2.465 · Cited by 915 · see citing works
Cationic liposome-mediated RNA transfection.
Malone, R. W., Felgner, P. L., & Verma, I. M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.16.6077 · Cited by 911 · see citing works
Karikó, K., Muramatsu, H., Ludwig, J., & Weissman, D. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr695 · Cited by 898 · see citing works
The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency.
Gallie, D. R. (1991) · Genes & Development · doi:10.1101/gad.5.11.2108 · Cited by 868 · see citing works
The RNA modification database, RNAMDB: 2011 update
Cantara, W. A., Crain, P. F., Rozenski, J., McCloskey, J. A., Harris, K. A., Zhang, X., Vendeix, F. A. P., Fabris, D., & Agris, P. F. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq1028 · Cited by 822 · see citing works
Expression of therapeutic proteins after delivery of chemically modified mRNA in mice
Kormann, M. S. D., Hasenpusch, G., Aneja, M. K., Nica, G., Flemmer, A. W., Herber-Jonat, S., Huppmann, M., Mays, L. E., Illenyi, M., Schams, A., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1733 · Cited by 733 · see citing works
MicroRNA Inhibition of Translation Initiation in Vitro by Targeting the Cap-Binding Complex eIF4F
Mathonnet, G., Fabian, M., Svitkin, Y., Parsyan, A., Huck, L., Murata, T., Biffo, S., Merrick, W., Darżynkiewicz, E., Pillai, R., et al. (2007) · Science · doi:10.1126/science.1146067 · Cited by 636 · see citing works
Holtkamp, S., Kreiter, S., Selmi, A., Simon, P., Koslowski, M., Huber, C., Türeci, O., & Sahin, U. (2006) · Blood · doi:10.1182/blood-2006-04-015024 · Cited by 625 · see citing works
Ligand-targeted liposome design: challenges and fundamental considerations
Noble, G. T., Stefanick, J. F., Ashley, J. D., Kiziltepe, T., & Bilgiçer, B. (2013) · Trends in biotechnology · doi:10.1016/j.tibtech.2013.09.007 · Cited by 543 · see citing works
A nuclear cap binding protein complex involved in pre-mRNA splicing
Izaurralde, E., Lewis, J., McGuigan, C., Jankowska, M., Darzynkiewicz, E., & Mattaj, I. W. (1994) · Cell · doi:10.1016/0092-8674(94)90530-4 · Cited by 517 · see citing works
Coller, J., & Parker, R. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.074032 · Cited by 484 · see citing works
5'-terminal cap structure in eucaryotic messenger ribonucleic acids
Banerjee, A. K. (1980) · Microbiological Reviews · doi:10.1128/mr.44.2.175-205.1980 · Cited by 477 · see citing works
Van Tendeloo, V. F. I., Ponsaerts, P., Lardon, F., Nijs, G., Lenjou, M., Van Broeckhoven, C., Van Bockstaele, D. R., & Berneman, Z. N. (2001) · Blood · doi:10.1182/blood.v98.1.49 · Cited by 468 · see citing works
TAIL-seq: Genome-wide Determination of Poly(A) Tail Length and 3′ End Modifications
Chang, H., Lim, J., Ha, M., & Kim, V. N. (2014) · Molecular Cell · doi:10.1016/j.molcel.2014.02.007 · Cited by 454 · see citing works
The Control of mRNA Decapping and P-Body Formation
Franks, T. M., & Lykke‐Andersen, J. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.11.001 · Cited by 399 · see citing works
(2001) · PubMed Central · Cited by 342 · see citing works
(1995) · PubMed · Cited by 330 · see citing works
Balanced Codon Usage Optimizes Eukaryotic Translational Efficiency
Qian, W., Yang, J.-R., Pearson, N. M., Maclean, C. J., & Zhang, J. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002603 · Cited by 303 · see citing works
Sheridan, C. (2011) · Nature Biotechnology · doi:10.1038/nbt.1769 · Cited by 302 · see citing works
Gene Therapies for Cancer: Strategies, Challenges and Successes
Das, S. K., Menezes, M. E., Bhatia, S., Wang, X., Emdad, L., Sarkar, D., & Fisher, P. B. (2014) · Journal of Cellular Physiology · doi:10.1002/jcp.24791 · Cited by 285 · see citing works
Gallie, D. R. (1998) · Gene · doi:10.1016/s0378-1119(98)00318-7 · Cited by 280 · see citing works
The Role of the Poly(A) Sequence in Mammalian Messenger RN
Brawerman, G. (1981) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238109114634 · Cited by 252 · see citing works
mRNA as gene therapeutic: How to control protein expression
Tavernier, G., Andries, O., Demeester, J., Sanders, N. N., De Smedt, S. C., & Rejman, J. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2010.10.020 · Cited by 232 · see citing works
Bettinger, T. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.18.3882 · Cited by 224 · see citing works
Noncytopathic Sindbis virus RNA vectors for heterologous gene expression
Agapov, E. V., Frolov, I. V., Lindenbach, B. D., Prágai, B. M., Schlesinger, S., & Rice, C. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.22.12989 · Cited by 214 · see citing works
Preclinical Evaluation of TriMix and Antigen mRNA-Based Antitumor Therapy
Lint, S. V., Goyvaerts, C., Maenhout, S. K., Goethals, L., Disy, A., Benteyn, D., Pen, J. J., Bonehill, A., Heirman, C., Breckpot, K., et al. (2012) · Cancer Research · doi:10.1158/0008-5472.can-11-2957 · Cited by 210 · see citing works
Current prospects for mRNA gene delivery
Yamamoto, A., Kormann, M., Rosenecker, J., & Rudolph, C. (2008) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2008.09.016 · Cited by 193 · see citing works
Diken, M., Kreiter, S., Selmi, A., Britten, C. M., Huber, C. G., Türeci, Ö., & Şahin, U. (2011) · Gene Therapy · doi:10.1038/gt.2011.17 · Cited by 192 · see citing works
Wang, Y.-H., Su, H.-H., Yang, Y., Hu, Y.-X., Zhang, L., Blancafort, P., & Huang, L. (2013) · Molecular Therapy · doi:10.1038/mt.2012.250 · Cited by 189 · see citing works
5'-terminal cap structure in eucaryotic messenger ribonucleic acids.
Banerjee, A. K. (1980) · Microbiological Reviews · doi:10.1128/mmbr.44.2.175-205.1980 · Cited by 188 · see citing works
Modulation of mRNA stability as a novel therapeutic approach
Eberhardt, W., Doller, A., Akool, E.-S., & Pfeilschifter, J. (2007) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2007.01.002 · Cited by 173 · see citing works
Phua, K. K. L., Leong, K. W., & Nair, S. K. (2013) · Journal of Controlled Release · doi:10.1016/j.jconrel.2012.12.029 · Cited by 159 · see citing works
Cap-Poly(A) Synergy in Mammalian Cell-free Extracts
Michel, Y. M., Poncet, D., Pirón, M., Kean, K. M., & Borman, A. M. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m004304200 · Cited by 159 · see citing works
A cap binding protein that may mediate nuclear export of RNA polymerase II-transcribed RNAs.
Izaurralde, E., Stȩpiński, J., Darżynkiewicz, E., & Mattaj, I. W. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.118.6.1287 · Cited by 153 · see citing works
Reverse 5' caps in RNAs made in vitro by phage RNA polymerases.
Pasquinelli, A. E., Dahlberg, J. E., & Lund, E. (1995) · PubMed · Cited by 148 · see citing works
Sæbøe‐Larssen, S., Fossberg, E., & Gaudernack, G. (2002) · Journal of Immunological Methods · doi:10.1016/s0022-1759(01)00506-3 · Cited by 140 · see citing works
Strobel, I., Berchtold, S., Götze, A., Schulze, U., Schuler, G., & Steinkasserer, A. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301326 · Cited by 137 · see citing works
Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity
Phua, K. K. L., Staats, H. F., Leong, K. W., & Nair, S. K. (2014) · Scientific Reports · doi:10.1038/srep05128 · Cited by 123 · see citing works
Single-cell mRNA transfection studies: Delivery, kinetics and statistics by numbers
Leonhardt, C., Schwake, G., Stögbauer, T. R., Rappl, S., Kuhr, J.-T., Ligon, T. S., & Rädler, J. O. (2014) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2013.11.008 · Cited by 117 · see citing works
Cancer immunotherapy using RNA-loaded dendritic cells
Ponsaerts, P., Tendeloo, V. F. V., & Berneman, Z. N. (2003) · Clinical & Experimental Immunology · doi:10.1046/j.1365-2249.2003.02286.x · Cited by 114 · see citing works
Control of mRNA stability in eukaryotes
Newbury, S. F. (2005) · Biochemical Society Transactions · doi:10.1042/bst20060030 · Cited by 96 · see citing works
Song, L., & Ma, Z. (2001) · Current Gene Therapy · doi:10.2174/1566523013348814 · Cited by 94 · see citing works
Control of mRNA stability in eukaryotes
Newbury, S. F. (2006) · Biochemical Society Transactions · doi:10.1042/bst0340030 · Cited by 91 · see citing works
SCHWARTZ, D., Decker, C. J., & Parker, R. (2003) · RNA · doi:10.1261/rna.2171203 · Cited by 86 · see citing works
Sendai virus vectors as an emerging negative‐strand RNA viral vector system
Bitzer, M., Armeanu, S., Lauer, U. M., & Neubert, W. J. (2003) · The Journal of Gene Medicine · doi:10.1002/jgm.426 · Cited by 85 · see citing works
Drosophila miR2 Primarily Targets the m7GpppN Cap Structure for Translational Repression
Zdanowicz, A., Thermann, R., Kowalska, J., Jemielity, J., Duncan, K. E., Preiß, T., Darżynkiewicz, E., & Hentze, M. W. (2009) · Molecular Cell · doi:10.1016/j.molcel.2009.09.009 · Cited by 79 · see citing works
Nallagatla, S. R., Toroney, R., & Bevilacqua, P. C. (2008) · RNA Biology · doi:10.4161/rna.5.3.6839 · Cited by 78 · see citing works
Zohra, F. T., Chowdhury, E. H., Tada, S., Hoshiba, T., & Akaike, T. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.04.059 · Cited by 71 · see citing works
Huth, S., Hoffmann, F., von Gersdorff, K., Laner, A., Reinhardt, D., Rosenecker, J., & Rudolph, C. (2006) · The Journal of Gene Medicine · doi:10.1002/jgm.975 · Cited by 68 · see citing works
Optimized transfection of mRNA transcribed from a d(A/T)100 tail-containing vector
Elango, N., Elango, S., Shivshankar, P., & Katz, M. S. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.03.067 · Cited by 57 · see citing works
The transmembrane domain of diphtheria toxin improves molecular conjugate gene transfer
Fisher, K. J., & Wilson, J. M. (1997) · Biochemical Journal · doi:10.1042/bj3210049 · Cited by 55 · see citing works
Strenkowska, M., Kowalska, J., Łukaszewicz, M., Zuberek, J., Su, W., Rhoads, R. E., Darżynkiewicz, E., & Jemielity, J. (2010) · New Journal of Chemistry · doi:10.1039/b9nj00644c · Cited by 42 · see citing works
Multi-Level Kinetic Model of mRNA Delivery via Transfection of Lipoplexes
Ligon, T. S., Leonhardt, C., & Rädler, J. O. (2014) · PLoS ONE · doi:10.1371/journal.pone.0107148 · Cited by 35 · see citing works
Amano, T., Kajiwara, K., Yoshikawa, K., Morioka, J., Nomura, S., Fujisawa, H., Kato, S., Fujii, M., Fukui, M., Hinoda, Y., et al. (2007) · Journal of neurosurgery · doi:10.3171/jns.2007.106.4.638 · Cited by 33 · see citing works
Interaction of Poliovirus-Specific RNAs with HeLa Cells and E. coli
Koch, G. (1973) · Modern aspects of electrochemistry · doi:10.1007/978-3-642-65772-6_4 · Cited by 32 · see citing works
Transferrin-Lipoplexes with Protamine-Condensed DNA for Serum-Resistant Gene Delivery
de Ilarduya, C. T., Arangoa, M. A., & Düzgüneş, N. (2003) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(03)73022-5 · Cited by 26 · see citing works
In‐vivo particle mediated delivery of mRNA to mammalian tissues: ballistic and biologic effects
Sohn, R. L., Murray, M. T., Schwarz, K., Nyitray, J., Purray, P., Franko, A. P., Palmer, K. C., Diebel, L. N., & Dulchavsky, S. A. (2001) · Wound Repair and Regeneration · doi:10.1046/j.1524-475x.2001.00287.x · Cited by 25 · see citing works
In Vivo and In Vitro Analysis of Poly(A) Length Effects on mRNA Translation
Peng, J., Murray, E. L., & Schoenberg, D. R. (2008) · Methods in molecular biology · doi:10.1007/978-1-59745-033-1_15 · Cited by 23 · see citing works
Protection of mRNA against nucleases in cytoplasmic extracts of mouse sarcoma ascites cells
Coutts, M., Krówczyńska, A., & Brawerman, G. (1993) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/0167-4781(93)90241-5 · Cited by 23 · see citing works
Highly Efficient mRNA-Based Gene Transfer in Feeder-Free Cultured H9 Human Embryonic Stem Cells
Ponsaerts, P., van der Sar, S., Tendeloo, V. F. V., Jorens, P. G., Berneman, Z. N., & Singh, P. B. (2004) · Cloning and Stem Cells · doi:10.1089/clo.2004.6.211 · Cited by 23 · see citing works
Zhang, H., Zhang, L., Ren, J., Fan, L., Si, X., & Liu, W. (2006) · Cellular Immunology · doi:10.1016/j.cellimm.2006.05.004 · Cited by 19 · see citing works
RNA Transfection of Dendritic Cells
Grünebach, F., Müller, M., & Brossart, P. (2004) · Humana Press eBooks · doi:10.1385/1-59259-862-5:047 · Cited by 18 · see citing works
Hoerr, I. (2000) · doi:10.1002/1521-4141(200001)30:1
Flagged first, then by citations
Research works printed but not linked: 1
- Phua KK Leong KW Nair SK Transfection efficiency and transgene expression kinetics of mRNA delivered in naked and nanoparticle format J Control Release 2013 166 227 33 23306021 PMC3594075
Cited by
Sorted by citations
CRISPR/Cas9 therapeutics: progress and prospects
Li, T., Yang, Y., Qi, H., Cui, W., Zhang, L., Fu, X., He, X., Liu, M., Li, P., & Yu, T. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01309-7 · Cited by 770 · see citing works
Advances in COVID-19 mRNA vaccine development
Fang, E., Liu, X., Li, M., Zhang, Z., Song, L., Zhu, B., Wu, X., Liu, J., Zhao, D., & Li, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00950-y · Cited by 601 · see citing works
Polymeric Delivery of Therapeutic Nucleic Acids
Kumar, R., Chalarca, C. F. S., Bockman, M. R., Bruggen, C. V., Grimme, C. J., Dalal, R. J., Hanson, M. G., Hexum, J. K., & Reineke, T. M. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00997 · Cited by 370 · see citing works
Cancer‐Targeting Nanoparticles for Combinatorial Nucleic Acid Delivery
Vaughan, H. J., Green, J. J., & Tzeng, S. Y. (2019) · Advanced Materials · doi:10.1002/adma.201901081 · Cited by 257 · see citing works
Boosting intracellular delivery of lipid nanoparticle-encapsulated mRNA
Patel, S., Ashwanikumar, N., Robinson, E., DuRoss, A., Sun, C., Murphy-Benenato, K. E., Mihai, C., Almarsson, Ö., & Sahay, G. (2017) · Nano Letters · doi:10.1021/acs.nanolett.7b02664 · Cited by 251 · see citing works
Mechanism of action of mRNA-based vaccines
Iavarone, C., O’hagan, D. T., Yu, D., Delahaye, N. F., & Ulmer, J. B. (2017) · Expert Review of Vaccines · doi:10.1080/14760584.2017.1355245 · Cited by 234 · see citing works
Paving the Road for RNA Therapeutics
Dammes, N., & Peer, D. (2020) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2020.08.004 · Cited by 220 · see citing works
Kwon, H., Kim, M., Seo, Y., Moon, Y. S., Lee, H. J., Lee, K., & Lee, H. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2017.11.034 · Cited by 208 · see citing works
MicroRNAs Enable mRNA Therapeutics to Selectively Program Cancer Cells to Self-Destruct
Jain, R., Frederick, J. P., Huang, E. Y., Burke, K. E., Mauger, D. M., Andrianova, E. A., Farlow, S. J., Siddiqui, S., Pimentel, J., Cheung-Ong, K., et al. (2018) · Nucleic Acid Therapeutics · doi:10.1089/nat.2018.0734 · Cited by 164 · see citing works
López‐Cebral, R., Silva‐Correia, J., Reis, R. L., Silva, T. H., & Oliveira, J. M. (2017) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.7b00655 · Cited by 139 · see citing works
Biomedical applications of mRNA nanomedicine
Xiong, Q., Lee, G. Y., Ding, J., Li, W., & Shi, J. (2018) · Nano Research · doi:10.1007/s12274-018-2146-1 · Cited by 125 · see citing works
Sayers, E. J., Peel, S. E., Schantz, A., England, R., Beano, M., Bates, S. M., Desai, A. S., Puri, S., Ashford, M. B., & Jones, A. T. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.07.018 · Cited by 117 · see citing works
Nguyen, M. K., Jeon, O., Dang, P. N., Huynh, C. T., Varghai, D., Riazi, H., McMillan, A., Herberg, S., & Alsberg, E. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.06.007 · Cited by 110 · see citing works
An overview of rational design of mRNA-based therapeutics and vaccines
To, K. K. W., & Cho, W. C. S. (2021) · Expert Opinion on Drug Discovery · doi:10.1080/17460441.2021.1935859 · Cited by 110 · see citing works
Inhaled RNA Therapy: From Promise to Reality
Chow, M. Y. T., Qiu, Y., & Lam, J. K. W. (2020) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2020.08.002 · Cited by 105 · see citing works
TCR Redirected T Cells for Cancer Treatment: Achievements, Hurdles, and Goals
Manfredi, F., Cianciotti, B. C., Potenza, A., Tassi, E., Noviello, M., Biondi, A., Ciceri, F., Bonini, C., & Ruggiero, E. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.01689 · Cited by 105 · see citing works
Leal, L., Guardo, A. C., Morón‐López, S., Salgado, M., Mothe, B., Heirman, C., Pannus, P., Vanham, G., van den Ham, H., Gruters, R. A., et al. (2018) · AIDS · doi:10.1097/qad.0000000000002026 · Cited by 100 · see citing works
Gene therapy to enhance angiogenesis in chronic wounds
Shaabani, E., Sharifiaghdam, M., Majidi, R. F., Smedt, S. C. D., Braeckmans, K., & Fraire, J. C. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.08.020 · Cited by 99 · see citing works
Lou, B., Koker, S. D., Lau, C. Y. J., Hennink, W. E., & Mastrobattista, E. (2018) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.8b00524 · Cited by 91 · see citing works
Golombek, S., Pilz, M., Steinle, H., Kochba, E., Levin, Y., Lunter, D., Schlensak, C., Wendel, H. P., & Avci-Adali, M. (2018) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2018.03.005 · Cited by 88 · see citing works
Maiyo, F., & Singh, M. (2019) · Pharmaceuticals · doi:10.3390/ph12040164 · Cited by 84 · see citing works
Moradian, H., Roch, T., Lendlein, A., & Gossen, M. (2020) · Scientific Reports · doi:10.1038/s41598-020-60506-4 · Cited by 73 · see citing works
Zha, W., Wang, J., Guo, Z., Zhang, Y., Wang, Y., Dong, S., Liu, C., Xing, H., & Li, X. (2022) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2022.122565 · Cited by 74 · see citing works
The mRNA Vaccine Revolution: COVID-19 Has Launched the Future of Vaccinology
Ye, Z., Harmon, J., Ni, W., Li, Y., Wich, D., & Xu, Q. (2023) · ACS Nano · doi:10.1021/acsnano.2c12584 · Cited by 71 · see citing works
In vitro evolution of enhanced RNA replicons for immunotherapy
Li, Y., Teague, B., Zhang, Y., Su, Z., Porter, E., Dobosh, B., Wagner, T., Irvine, D. J., & Weiss, R. (2019) · Scientific Reports · doi:10.1038/s41598-019-43422-0 · Cited by 71 · see citing works
Enabling mRNA Therapeutics: Current Landscape and Challenges in Manufacturing
Youssef, M., Hitti, C., Fulber, J. P. C., & Kamen, A. (2023) · Biomolecules · doi:10.3390/biom13101497 · Cited by 62 · see citing works
Zhang, X., Men, K., Zhang, Y., Zhang, R., Yang, L., & Duan, X. (2019) · International Journal of Nanomedicine · doi:10.2147/ijn.s198747 · Cited by 57 · see citing works
Lipid-Based Vectors for Therapeutic mRNA-Based Anti-Cancer Vaccines
Guevara, M. L., Persano, S., & Persano, F. (2019) · Current Pharmaceutical Design · doi:10.2174/1381612825666190619150221 · Cited by 56 · see citing works
Immunological Analysis of a CCHFV mRNA Vaccine Candidate in Mouse Models
Farzani, T. A., Földes, K., Ergünay, K., Gürdal, H., Baştuğ, A., & Özkul, A. (2019) · Vaccines · doi:10.3390/vaccines7030115 · Cited by 55 · see citing works
A scalable and robust cationic lipid/polymer hybrid nanoparticle platform for mRNA delivery
Meyer, R. A., Hussmann, G. P., Peterson, N. C., Santos, J. L., & Tuesca, A. D. (2021) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2021.121314 · Cited by 55 · see citing works
Firdessa-Fite, R., & Creusot, R. J. (2019) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2019.10.015 · Cited by 53 · see citing works
Modulating intracellular pathways to improve non-viral delivery of RNA therapeutics
de Vyver, T. V., Smedt, S. C. D., & Raemdonck, K. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.114041 · Cited by 51 · see citing works
NK Cells Armed with Chimeric Antigen Receptors (CAR): Roadblocks to Successful Development
Dezfouli, A. B., Yazdi, M., Pockley, A. G., Khosravi, M., Kobold, S., Wagner, E., & Multhoff, G. (2021) · Cells · doi:10.3390/cells10123390 · Cited by 51 · see citing works
Kramps, T., & Elbers, K. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-6481-9_1 · Cited by 50 · see citing works
Dynamic mRNA polyplexes benefit from bioreducible cleavage sites for in vitro and in vivo transfer
Levačić, A. K., Berger, S., Müller, J., Wegner, A., Lächelt, U., Dohmen, C., Rudolph, C., & Wagner, E. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.09.016 · Cited by 47 · see citing works
mRNA Vaccines: Why Is the Biology of Retroposition Ignored?
Domazet‐Lošo, T. (2022) · Genes · doi:10.3390/genes13050719 · Cited by 44 · see citing works
Du, R., Wang, C., Zhu, L., & Yang, Y. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14102236 · Cited by 44 · see citing works
Hepatocellular-Targeted mRNA Delivery Using Functionalized Selenium Nanoparticles In Vitro
Singh, D., & Singh, M. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13030298 · Cited by 44 · see citing works
Bioinspired cellular membrane-derived vesicles for mRNA delivery
Xu, X., Xu, L., Wang, J., Wen, C., Xia, J., Zhang, Y., & Liang, Y. (2024) · Theranostics · doi:10.7150/thno.93755 · Cited by 41 · see citing works
Non-coding RNA delivery for bone tissue engineering: Progress, challenges, and potential solutions
Guan, S., Zhang, Z., & Wu, J. (2022) · iScience · doi:10.1016/j.isci.2022.104807 · Cited by 39 · see citing works
Cancer Vaccines in the Immunotherapy Era: Promise and Potential
Verma, C., Pawar, V. A., Srivastava, S., Tyagi, A., Kaushik, G., Shukla, S. K., & Kumar, V. (2023) · Vaccines · doi:10.3390/vaccines11121783 · Cited by 37 · see citing works
RNA ‐based therapies for genodermatoses
Bornert, O., Peking, P., Bremer, J., Koller, U., van den Akker, P. C., Aartsma-Rus, A. M., Pasmooij, A. M. G., Murauer, E. M., & Nyström, A. (2016) · Experimental Dermatology · doi:10.1111/exd.13141 · Cited by 37 · see citing works
Zhang, J., Liu, Y., Li, C., Xiao, Q., Zhang, D., Chen, Y., Rosenecker, J., Ding, X., & Guan, S. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15092182 · Cited by 39 · see citing works
Wang, K., Wang, X., Jiang, D. M., Pei, Y., Wang, Z., Zhou, X., Wu, J., Mo, X., & Wang, H. (2022) · Composites Part B Engineering · doi:10.1016/j.compositesb.2022.109648 · Cited by 35 · see citing works
Personalized mRNA vaccines in glioblastoma therapy: from rational design to clinical trials
Karimi-Sani, I., Molavi, Z., Naderi, S., Mirmajidi, S. H., Zare, I., Naeimzadeh, Y., Mansouri, A., Tajbakhsh, A., Savardashtaki, A., & Sahebkar, A. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02882-x · Cited by 38 · see citing works
Kafshgari, M. H., Agiotis, L., Largillière, I., Patskovsky, S., & Michel Meunier, A. (2021) · Small · doi:10.1002/smll.202007577 · Cited by 31 · see citing works
Jabłońska, A., Shea, D. J., Cao, S., Bulte, J. W. M., Janowski, M., Κωνσταντόπουλος, Κ., & Walczak, P. (2017) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x17703888 · Cited by 31 · see citing works
Recent Advances and Prospects of Nucleic Acid Therapeutics for Anti-Cancer Therapy
Lee, M., Lee, M.-J., Song, Y., Kim, S., & Park, N. (2024) · Molecules · doi:10.3390/molecules29194737 · Cited by 29 · see citing works
Beigi, A., Naghib, S. M., Matini, A., Tajabadi, M., & Mozafari, M. R. (2024) · Current Gene Therapy · doi:10.2174/0115665232292768240503050508 · Cited by 28 · see citing works
Gonzalez-Fernandez, T., Kelly, D., & O'Brien, F. (2018) · Advances in Therapy · doi:10.1002/adtp.201800038 · Cited by 24 · see citing works
Oliveira, A. C., Fernandes, J., Gonçalves, A., Gomes, A. C., & Oliveira, M. E. C. D. R. (2018) · Current Drug Targets · doi:10.2174/1389450119666180703145410 · Cited by 25 · see citing works
Development of an RNA Expression Platform Controlled by Viral Internal Ribosome Entry Sites
Ko, H. L., Park, H., Kim, J., Kim, H., Youn, H., & Nam, J. (2019) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1811.11019 · Cited by 22 · see citing works
Self-citationPhysical transfection technologies for macrophages and dendritic cells in immunotherapy
Harizaj, A., Smedt, S. C. D., Lentacker, I., & Braeckmans, K. (2020) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2021.1828340 · Cited by 21 · see citing works
mRNA Vaccine Platform: mRNA Production and Delivery
Litvinova, V. R., Рудометов, А. П., Карпенко, Л. И., & Ilyichev, A. A. (2023) · Russian Journal of Bioorganic Chemistry · doi:10.1134/s1068162023020152 · Cited by 21 · see citing works
Granot-Matok, Y., Ezra, A., Ramishetti, S., Sharma, P., Naidu, G. S., Benhar, I., & Peer, D. (2023) · Theranostics · doi:10.7150/thno.82228 · Cited by 20 · see citing works
Shah, S. A., Sohail, M. F., Nakielski, P., Rinoldi, C., Zargarian, S. S., Kosik‐Kozioł, A., Ziai, Y., Bayan, M. A. H., Zakrzewska, A., Rybak, D., et al. (2024) · Biomacromolecules · doi:10.1021/acs.biomac.4c01133 · Cited by 18 · see citing works
Chávez, J. C. P., McGrath, M., Kearney, C. J., Browne, S., & O’Brien, F. J. (2025) · Cell Biomaterials · doi:10.1016/j.celbio.2025.100073 · Cited by 18 · see citing works
Chambers, P., Ziminska, M., Elkashif, A., Wilson, J., Redmond, J., Tzagiollari, A., Ferreira, C., Balouch, A. P., Bogle, J., Donahue, S. W., et al. (2023) · Journal of Controlled Release · doi:10.1016/j.jconrel.2023.09.006 · Cited by 18 · see citing works
Włodarczyk, J., Leng, A., Abadchi, S. N., Shababi, N., Mokhtari‐Esbuie, F., Gheshlaghi, S., Ravari, M. R., Pippenger, E. K., Afrasiabi, A., Ha, J. S., et al. (2024) · Scientific Reports · doi:10.1038/s41598-024-54941-w · Cited by 17 · see citing works
Denzinger, M., Link, A., Kurz, J., Krauß, S., Thoma, R., Schlensak, C., Wendel, H. P., & Krajewski, S. (2018) · Nucleic Acid Therapeutics · doi:10.1089/nat.2018.0737 · Cited by 17 · see citing works
Lim, J., Oh, D., Cheng, M., Chintapula, U. K., Liu, S., Reynolds, D., Zhang, X., Zhou, Y., Xu, X., & Ko, J. (2025) · Small · doi:10.1002/smll.202410975 · Cited by 16 · see citing works
mRNA therapeutics for disease therapy: principles, delivery, and clinical translation
Zhou, D., Wang, K., Zhang, Y., Ma, K., Yang, X., Li, Z.-Y., Yu, S., Chen, K., & Qiao, S. (2023) · Journal of Materials Chemistry B · doi:10.1039/d2tb02782h · Cited by 17 · see citing works
Satyal, U., Draghici, B., Dragic, L., Zhang, Q., Norris, K. W., Madesh, M., Brailoiu, E., & Ilies, M. A. (2017) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.7b07066 · Cited by 15 · see citing works
Vectored gene delivery for lifetime animal contraception: Overview and hurdles to implementation
Hay, B. A., Li, J., & Guo, M. (2017) · Theriogenology · doi:10.1016/j.theriogenology.2017.11.003 · Cited by 15 · see citing works
Delivery strategies of RNA therapeutics to leukocytes
Tarab‐Ravski, D., Stotsky‐Oterin, L., & Peer, D. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.01.016 · Cited by 15 · see citing works
Plasmid DNA and mRNA delivery: Approaches and challenges
Singh, A. K., Goel, K., & Dhanawat, M. (2025) · Advances in immunology · doi:10.1016/bs.ai.2024.12.001 · Cited by 13 · see citing works
Context base editing for splice correction of IVSI-110 β-thalassemia
Naiisseh, B., Papasavva, P. L., Papaioannou, N. Y., Tomazou, M., Koniali, L., Felekis, X., Constantinou, C. G., Sitarou, M., Christou, S., Kleanthous, M., et al. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102183 · Cited by 13 · see citing works
Chatterjee, S., Bhattacharya, M., Lee, S., & Chakraborty, C. (2023) · Current Research in Biotechnology · doi:10.1016/j.crbiot.2023.100150 · Cited by 12 · see citing works
Development of a Novel Polymer-Based mRNA Coating for Surgical Suture to Enhance Wound Healing
Link, A., Haag, H., Michel, T., Denzinger, M., Wendel, H. P., Schlensak, C., & Krajewski, S. (2019) · Coatings · doi:10.3390/coatings9060374 · Cited by 11 · see citing works
Hoffmann, M., Gerlach, S., Takamiya, M., Tarazi, S., Hersch, N., Csiszár, Á., Springer, R., Dreissen, G., Scharr, H., Rastegar, S., et al. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15041210 · Cited by 11 · see citing works
Self‐Organization of Iron Sulfide Nanoparticles into Complex Multicompartment Supraparticles
Emre, E. S. T., Emre, A. E., Vecchio, D. A., Kadiyala, U., VanEpps, J. S., & Kotov, N. A. (2023) · Advanced Materials · doi:10.1002/adma.202211244 · Cited by 12 · see citing works
Biodegradable Dendronized Polymers for Efficient mRNA Delivery
Oldenhuis, N. J., Eldredge, A. C., Burts, A. O., Ryu, K. A., Chung, J., Johnson, M. E., & Guan, Z. (2016) · ChemistrySelect · doi:10.1002/slct.201600939 · Cited by 10 · see citing works
Self-citationNucleic Acid Nanocapsules as a New Platform to Deliver Therapeutic Nucleic Acids for Gene Regulation
Pal, S., Cannata, J. N., & Rouge, J. L. (2025) · Accounts of Chemical Research · doi:10.1021/acs.accounts.5c00126 · Cited by 8 · see citing works
Prime editing in mammals: From promise to practicalities
Brooks, I. R., Graham, C., Kazemizadeh, A., & Jackow, J. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102719 · Cited by 8 · see citing works
Seong, S., Vijayan, V., Kim, J. H., Kim, K., Kim, I., Cherukula, K., Park, I., & Kim, N. (2023) · Biomaterials Science · doi:10.1039/d2bm02038f · Cited by 8 · see citing works
Анатольевич, Г. А., Savkina, M. V., Obukhov, Y. I., Меркулов, В. А., & Olefir, Y. V. (2019) · Biological Products Prevention Diagnosis Treatment · doi:10.30895/2221-996x-2019-19-2-72-80 · Cited by 8 · see citing works
Oyama, N., Takahashi, H., Kawaguchi, M., Miyamoto, H., Nishida, K., Tsurumaru, M., Nakashima, M., Yamashita, F., Hashida, M., & Kawakami, S. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12020114 · Cited by 8 · see citing works
Polymeric Vehicles for Nucleic Acid Delivery: Enhancing the Therapeutic Efficacy and Cellular Uptake
Gupta, P., Sharma, A., & Mittal, V. (2024) · Recent Advances in Drug Delivery and Formulation · doi:10.2174/0126673878324536240805060143 · Cited by 7 · see citing works
Supramolecular Gene Transfection Agents
Pitard, B., & Habrant, D. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-409547-2.12563-6 · Cited by 7 · see citing works
Next-Generation Therapeutics: mRNA as a Novel Therapeutic Option for Single-Gene Disorders
Michel, T., Wendel, H.-P., & Krajewski, S. (2016) · InTech eBooks · doi:10.5772/62243 · Cited by 6 · see citing works
Moradian, H., Dunne, C. M., Gossen, M., & Hartlieb, M. (2025) · Advanced Science · doi:10.1002/advs.202512653 · Cited by 6 · see citing works
Michel, T., Link, A., Abraham, M.-K., Schlensak, C., Peter, K., Wendel, H.-P., Wang, X., & Krajewski, S. (2019) · Journal of Visualized Experiments · doi:10.3791/58444 · Cited by 7 · see citing works
Chen, F., Liu, Q., Xiong, Y., & Xu, L. (2022) · Frontiers in Pharmacology · doi:10.3389/fphar.2022.984981 · Cited by 5 · see citing works
Gram‐Scale Chemical Synthesis of Base‐Modified Ribonucleoside‐5′‐O‐Triphosphates
Shanmugasundaram, M., Senthilvelan, A., & Kore, A. R. (2016) · Current Protocols in Nucleic Acid Chemistry · doi:10.1002/cpnc.20 · Cited by 4 · see citing works
Modeling the intracellular dynamics of the dengue viral infection and the innate immune response.
Castaño-Arcila, M., Aguilera, L. U., & Rodríguez-González, J. (2020) · Journal of Theoretical Biology · doi:10.1016/j.jtbi.2020.110529 · Cited by 3 · see citing works
mRNA vaccines: Why and how they should be modified
Feliciello, I., & Procino, A. (2021) · Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale · doi:10.4081/jbr.2021.10072 · Cited by 4 · see citing works
TRAIL Therapy for Breast Cancer Treatment by Employing Lipopolymer mRNA Delivery
Thapa, B., Remant, K. C., Liu, X., Fu, W. E., Seol, D., & Uludağ, H. (2022) · GEN Biotechnology · doi:10.1089/genbio.2021.0007 · Cited by 4 · see citing works
Pareek, A., Modi, D., Kapoor, D. U., Prajapati, B. G., Fareed, M., & Alsaidan, O. A. (2026) · International Journal of Polymeric Materials · doi:10.1080/00914037.2025.2610712 · Cited by 3 · see citing works
Deconvolution of synthetic mRNA expression: Nucleoside chemistry alters translatability
Moradian, H., Schwestka, M., Roch, T., & Gossen, M. (2023) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10622 · Cited by 3 · see citing works
Key to Delivery: The (Epi-)genome Editing Vector Toolbox
Just, S., & Büning, H. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-7774-1_7 · Cited by 3 · see citing works
Sithole, M. N., Khan, M. R., Mohammed, H. A., Khan, R. A., Naik, K., & Choonara, Y. E. (2026) · Virus Research · doi:10.1016/j.virusres.2026.199720 · Cited by 2 · see citing works
Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter
Butterfield, K., McGrath, P. S., Han, C. M., Kogut, I., & Bilousova, G. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-0655-1_2 · Cited by 2 · see citing works
mRNA-Vaccine Platform: Features of Obtaining and Delivery of mRNA
Litvinova, V. R., Рудометов, А. П., Карпенко, Л. И., & Ilyichev, A. A. (2023) · Биоорганическая химия · doi:10.31857/s013234232302015x · Cited by 2 · see citing works
Kreofsky, N. W., Roy, P., Ghosh, R., Panda, S., Sarısözen, C., & Reineke, T. M. (2025) · Biomacromolecules · doi:10.1021/acs.biomac.5c00901 · Cited by 1 · see citing works
mRNA nanotherapeutics for sepsis: immune modulation strategies and translational challenges
Liu, Y., Wang, K., Gao, F., Bai, X., Yang, J., Li, Z., & Wang, Y.-C. (2026) · Journal of Nanobiotechnology · doi:10.1186/s12951-026-04366-6 · Cited by 1 · see citing works
Tanskanen, E., Sun, H., Cheng, K.-C., Ishihara, J., & Patel, A. K. (2026) · RSC Pharmaceutics · doi:10.1039/d6pm00136j · Cited by 1 · see citing works
Less is more: slow-codon windows enhance eGFP mRNA resilience against RNA interference
Müller, J., Schwake, G., Reiser, A., Woschée, D., Alirezaeizanjani, Z., Rädler, J. O., & Rudorf, S. (2025) · Journal of The Royal Society Interface · doi:10.1098/rsif.2024.0582
Gene Therapy in Cardiac Disease
Vakrou, S., & Malliaras, K. G. (2019) · Springer eBooks · doi:10.1007/978-3-319-98186-4_18
mRNA AŞILARINDA GÜNCEL YAKLAŞIMLAR
BATUR, K., & Yardımcı, H. (2022) · Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni · doi:10.38137/vftd.1021843
A stochastic model for hepatitis C viral infection dynamics with the innate immune response
Castaño-Arcila, M., & Rodríguez-González, J. (2022) · Elsevier eBooks · doi:10.1016/b978-0-32-390171-0.00018-4
- Authors
- Youn, H., & Chung, J.-K.
- Affiliations
- Seoul National University (KR)
- Corresponding author
- Chung, June-Key
- Venue
- Expert Opinion on Biological Therapy
- Publisher
- Informa UK Limited
- Published
- 2015-06-30
- Volume
- 15
- Issue
- 9
- Pages
- 1337-1348
- ISSN
- 1471-2598 (ISSN-L)
- 1744-7682
- PMC
- PMC4696419
- OpenAlex
- W1667670121
- Cited by
- 114
- References
- 73
- Citations by year
- 2026: 11 · 2025: 11 · 2024: 8 · 2023: 17 · 2022: 12 · 2021: 10 · 2020: 8 · 2019: 14 · 2018: 10 · 2017: 7 · 2016: 6
- FWCI
- 0.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Expert Opinion on Biological Therapy (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Deutsches Krebsforschungszentrum
- National Research Foundation (2011-0030001)
- Seoul National University (2011-0030001)
- Ministry of Science, ICT and Future Planning (2011‐0030001)
- Korea Health Industry Development Institute (HI14C1072, HI13C0826)
- National Research Foundation of Korea (2011‐0030001, HI14C1072)
- Seoul National University Hospital
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- mRNA modification, plasmid DNA, gene therapy, therapeutic vaccination, mRNA delivery, transfection efficiency, immunogenicity, in vivo gene therapy
- MeSH terms
- Animals, Humans, Nucleosides, Plasmids, RNA Caps, RNA, Messenger, Transfection, Vaccination, Genetic Therapy, Immunotherapy, Active, Untranslated Regions
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