Opportunities and Challenges in the Delivery of mRNA-Based Vaccines
Open accessAbstract
In the past few years, there has been increasing focus on the use of messenger RNA (mRNA) as a new therapeutic modality. Current clinical efforts encompassing mRNA-based drugs are directed toward infectious disease vaccines, cancer immunotherapies, therapeutic protein replacement therapies, and treatment of genetic diseases. However, challenges that impede the successful translation of these molecules into drugs are that (i) mRNA is a very large molecule, (ii) it is intrinsically unstable and prone to degradation by nucleases, and (iii) it activates the immune system. Although some of these challenges have been partially solved by means of chemical modification of the mRNA, intracellular delivery of mRNA still represents a major hurdle. The clinical translation of mRNA-based therapeutics requires delivery technologies that can ensure stabilization of mRNA under physiological conditions. Here, we (i) review opportunities and challenges in the delivery of mRNA-based therapeutics with a focus on non-viral delivery systems, (ii) present the clinical status of mRNA vaccines, and (iii) highlight perspectives on the future of this promising new type of medicine.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
At present, the 3′ UTR is mainly derived from hemoglobin subunit α (HBA) and subunit β (HBB) genes, but it can also be derived from albumin (ALB), heat-shock protein 70 (Hsp70), tyrosine hydroxylase (TH), and collagen alpha 1 (COL1A1) genes.
“At present, the 3′ UTR is mainly derived from hemoglobin subunit α (HBA) and subunit β (HBB) genes,140 but it can also be derived from albumin (ALB), heat-shock protein 70 (Hsp70), tyrosine hydroxylase (TH), and collagen alpha 1 (COL1A1) genes.141–143”
From Advances in COVID-19 mRNA vaccine development (2022) · cites this paper as [140]
The 5′ cap protects mRNA sterically from degradation by exonucleases and works synergistically with the poly(A) tail, poly(A) binding proteins and translation initiation factor proteins to circularize mRNA and recruit ribosomes for initiating translation.
“Further, the 5′ cap protects the mRNA sterically from degradation by exonucleases, and it works synergistically with the poly(A) tail at the 3′ end, poly(A) binding proteins and translation initiation factor proteins to circularize mRNA and recruit ribosomes for initiating translation10,11.”
From mRNA vaccines for infectious diseases: principles, delivery and clinical translation (2021) · cites this paper as [10]
2′-O-methylation of the first or second nucleotide from the 5′ end prevents recognition by cytosolic sensors of viral RNA and hence prevents unintended immune responses.
“As in mammals, the first or second nucleotide from the 5′ end is methylated on the 2′ hydroxyl of the ribose (2′-O-methylation), which prevents recognition by cytosolic sensors of viral RNA10, and hence prevents unintended immune responses.”
From mRNA vaccines for infectious diseases: principles, delivery and clinical translation (2021) · cites this paper as [10]
The 5′ and 3′ UTRs flanking the coding region regulate mRNA translation, half-life and subcellular localization.
“The 5′ and 3′ UTRs flanking the coding region regulate mRNA translation, half-life and subcellular localization10,14.”
From mRNA vaccines for infectious diseases: principles, delivery and clinical translation (2021) · cites this paper as [10]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
mRNA vaccines — a new era in vaccinology
Pardi, N., Hogan, M. J., Porter, F. W., & Weissman, D. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2017.243 · Cited by 4,667 · see citing works
Direct Gene Transfer into Mouse Muscle in Vivo
Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L. (1990) · Science · doi:10.1126/science.1690918 · Cited by 4,173 · cited 2× · see citing works
“The development of mRNA‐based drugs dates back to 1990 with the successful expression of a number of different proteins upon injecting mRNA encoding these proteins directly into the muscles of mice [24].”
BackgroundLiposome: classification, preparation, and applications
Akbarzadeh, A., Rezaei-Sadabady, R., Davaran, S., Joo, S. W., Zarghami, N., Hanifehpour, Y., Samiei, M., Kouhi, M., & Nejati‐Koshki, K. (2013) · Nanoscale Research Letters · doi:10.1186/1556-276x-8-102 · Cited by 3,648 · see citing works
Mechanisms of type-I- and type-II-interferon-mediated signalling
Platanias, L. C. (2005) · Nature reviews. Immunology · doi:10.1038/nri1604 · Cited by 3,480 · see citing works
Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA
Diebold, S. S., Kaisho, T., Hemmi, H., Akira, S., & Reis e Sousa, C. (2004) · Science · doi:10.1126/science.1093616 · Cited by 3,444 · see citing works
mRNA-based therapeutics--developing a new class of drugs
Sahin, U., Karikó, K., & Türeci, Ö. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4278 · Cited by 2,474 · see citing works
Toxicity of cationic lipids and cationic polymers in gene delivery
Lv, H., Zhang, S., Wang, B., Cui, S., & Yan, J. (2006) · Journal of Controlled Release · doi:10.1016/j.jconrel.2006.04.014 · Cited by 2,286 · see citing works
The alphaviruses: gene expression, replication, and evolution.
Strauss, J. H., & Strauss, E. G. (1994) · Microbiological Reviews · doi:10.1128/mmbr.58.3.491-562.1994 · Cited by 2,221 · see citing works
Karikó, K., Muramatsu, H., Welsh, F. A., Ludwig, J., Kato, H., Akira, S., & Weissman, D. (2008) · Molecular Therapy · doi:10.1038/mt.2008.200 · Cited by 1,930 · see citing works
Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapy
Kranz, L. M., Diken, M., Haas, H., Kreiter, S., Loquai, C., Reuter, K. C., Meng, M., Fritz, D., Vascotto, F., Hefesha, H., et al. (2016) · Nature · doi:10.1038/nature18300 · Cited by 1,771 · see citing works
The Many Pathways of RNA Degradation
Houseley, J. M., & Tollervey, D. W. (2009) · Cell · doi:10.1016/j.cell.2009.01.019 · Cited by 1,220 · see citing works
Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery
Kowalski, P. S., Rudra, A., Miao, L., & Anderson, D. G. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.02.012 · Cited by 1,127 · see citing works
Pardi, N., Tuyishime, S., Muramatsu, H., Kariko, K., Mui, B. L., Tam, Y. K., Madden, T. D., Hope, M. J., & Weissman, D. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.08.007 · Cited by 1,014 · see citing works
Polyethylenimine-based non-viral gene delivery systems
Lungwitz, U., Breunig, M., Blunk, T., & Göpferich, A. M. (2005) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2004.11.011 · Cited by 985 · see citing works
Cationic liposome-mediated RNA transfection.
Malone, R. W., Felgner, P. L., & Verma, I. M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.16.6077 · Cited by 911 · see citing works
mRNA capping: biological functions and applications
Ramanathan, A., Robb, G. B., & Chan, S. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw551 · Cited by 892 · see citing works
Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination
Pardi, N., Hogan, M. J., Pelc, R. S., Muramatsu, H., Andersen, H., DeMaso, C. R., Dowd, K. A., Sutherland, L. L., Scearce, R. M., Parks, R., et al. (2017) · Nature · doi:10.1038/nature21428 · Cited by 884 · see citing works
Tools for translation: non-viral materials for therapeutic mRNA delivery
Hajj, K. A., & Whitehead, K. A. (2017) · Nature Reviews Materials · doi:10.1038/natrevmats.2017.56 · Cited by 799 · see citing works
Kauffman, K. J., Dorkin, J. R., Yang, J. H., Heartlein, M. W., DeRosa, F., Mir, F. F., Fenton, O. S., & Anderson, D. G. (2015) · Nano Letters · doi:10.1021/acs.nanolett.5b02497 · Cited by 785 · see citing works
Non Viral Vectors in Gene Therapy- An Overview
Ramamoorth, M. (2015) · JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH · doi:10.7860/jcdr/2015/10443.5394 · Cited by 771 · see citing works
Expression of therapeutic proteins after delivery of chemically modified mRNA in mice
Kormann, M. S. D., Hasenpusch, G., Aneja, M. K., Nica, G., Flemmer, A. W., Herber-Jonat, S., Huppmann, M., Mays, L. E., Illenyi, M., Schams, A., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1733 · Cited by 733 · see citing works
Miao, L., Li, L., Huang, Y., Delcassian, D., Chahal, J., Han, J., Shi, Y., Sadtler, K., Gao, W., Lin, J., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0247-3 · Cited by 723 · see citing works
Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation
Anderson, B. R., Muramatsu, H., Nallagatla, S. R., Bevilacqua, P. C., Sansing, L. H., Weissman, D., & Karikó, K. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq347 · Cited by 682 · see citing works
Advances in mRNA Vaccines for Infectious Diseases
Zhang, C., Maruggi, G., Shan, H., & Li, J. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.00594 · Cited by 680 · see citing works
Gene delivery by lipoplexes and polyplexes
de Ilarduya, C. T., Sun, Y., & Düzgüneş, N. (2010) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2010.03.019 · Cited by 626 · see citing works
Holtkamp, S., Kreiter, S., Selmi, A., Simon, P., Koslowski, M., Huber, C., Türeci, O., & Sahin, U. (2006) · Blood · doi:10.1182/blood-2006-04-015024 · Cited by 625 · see citing works
mRNA vaccine delivery using lipid nanoparticles
Reichmuth, A. M., Oberli, M. A., Jaklenec, A., Langer, R., & Blankschtein, D. (2016) · Therapeutic Delivery · doi:10.4155/tde-2016-0006 · Cited by 624 · see citing works
Developing mRNA-vaccine technologies
Schlake, T., Thess, A., Fotin-Mleczek, M., & Kallen, K.-J. (2012) · RNA Biology · doi:10.4161/rna.22269 · Cited by 622 · see citing works
Sharova, L. V., Sharov, A. A., Nedorezov, T., Piao, Y., Shaik, N., & Ko, M. S. H. (2009) · DNA Research · doi:10.1093/dnares/dsn030 · Cited by 591 · see citing works
A facile method for the removal of dsRNA contaminant from in vitro-transcribed mRNA
Baiersdörfer, M., Boros, G., Muramatsu, H., Mahiny, A., Vlatkovic, I., Sahin, U., & Karikó, K. (2019) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2019.02.018 · Cited by 569 · see citing works
Recognition of Endogenous Nucleic Acids by the Innate Immune System
Roers, A., Hiller, B., & Hornung, V. (2016) · Immunity · doi:10.1016/j.immuni.2016.04.002 · Cited by 541 · see citing works
Transdermal Delivery of Drugs with Microneedles—Potential and Challenges
Ita, K. B. (2015) · Pharmaceutics · doi:10.3390/pharmaceutics7030090 · Cited by 527 · see citing works
Gary, D. J., Puri, N., & Won, Y. (2007) · Journal of Controlled Release · doi:10.1016/j.jconrel.2007.05.021 · Cited by 514 · see citing works
mRNA as a Transformative Technology for Vaccine Development to Control Infectious Diseases
Maruggi, G., Zhang, C., Li, J., Ulmer, J. B., & Yu, D. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.01.020 · Cited by 507 · see citing works
Vogel, A. B., Lambert, L., Kinnear, E., Busse, D., Erbar, S., Reuter, K. C., Wicke, L., Perkovic, M., Beissert, T., Haas, H., et al. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2017.11.017 · Cited by 501 · see citing works
Mukherjee, A., Waters, A. K., Kalyan, P., Achrol, A. S., Kesari, S., & Yenugonda, V. M. (2019) · International Journal of Nanomedicine · doi:10.2147/ijn.s198353 · Cited by 499 · see citing works
Coller, J., & Parker, R. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.074032 · Cited by 484 · see citing works
Induction of virus‐specific cytotoxic T lymphocytes <i>in vivo</i> by liposome‐entrapped mRNA
Martinon, F., Krishnan, S., Lenzen, G., Magné, R., Gomard, E., Guillet, J., Lévy, J., & Meulien, P. (1993) · European Journal of Immunology · doi:10.1002/eji.1830230749 · Cited by 473 · see citing works
An mRNA m7G Cap Binding-like Motif within Human Ago2 Represses Translation
Kiriakidou, M., Tan, G. S., Lamprinaki, S., Planell-Saguer, M. D., Nelson, P. T., & Mourelatos, Z. (2007) · Cell · doi:10.1016/j.cell.2007.05.016 · Cited by 462 · see citing works
Zhao, Y., Moon, E. K., Carpenito, C., Paulos, C. M., Liu, X., Brennan, A. L., Chew, A., Carroll, R. G., Scholler, J., Levine, B. L., et al. (2010) · Cancer Research · doi:10.1158/0008-5472.can-10-2880 · Cited by 448 · see citing works
Filion, M. C., & Phillips, N. C. (1997) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(97)00126-0 · Cited by 428 · see citing works
Monomethylated cap structures facilitate RNA export from the nucleus
Hamm, J., & Mattaj, I. W. (1990) · Cell · doi:10.1016/0092-8674(90)90292-m · Cited by 413 · see citing works
Nanotechnologies in delivery of mRNA therapeutics using nonviral vector-based delivery systems
Guan, S., & Rosenecker, J. (2017) · Gene Therapy · doi:10.1038/gt.2017.5 · Cited by 389 · see citing works
mRNA poly(A) tail, a 3' enhancer of translational initiation.
Munroe, D. J., & Jacobson, A. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.7.3441 · Cited by 351 · see citing works
Polymer–Lipid Nanoparticles for Systemic Delivery of mRNA to the Lungs
Kaczmarek, J. C., Patel, A. K., Kauffman, K. J., Fenton, O. S., Webber, M. J., Heartlein, M. W., DeRosa, F., & Anderson, D. G. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201608450 · Cited by 337 · see citing works
Fotin-Mleczek, M., Duchardt, K. M., Lorenz, C., Pfeiffer, R., Ojkić-Zrna, S., Probst, J., & Kallen, K.-J. (2011) · Journal of Immunotherapy · doi:10.1097/cji.0b013e3181f7dbe8 · Cited by 335 · cited 1× · see citing works
“This may be accomplished by introducing adenylate‐uridylate‐rich elements in the 3’‐UTRs of the mRNA, eventually resulting in faster mRNA degradation and shortening of the duration of protein expression [63].”
BackgroundLipid Nanoparticle Formulations for Enhanced Co-delivery of siRNA and mRNA
Ball, R. L., Hajj, K. A., Vizelman, J., Bajaj, P., & Whitehead, K. A. (2018) · Nano Letters · doi:10.1021/acs.nanolett.8b01101 · Cited by 314 · see citing works
Kreiter, S., Selmi, A., Diken, M., Koslowski, M., Britten, C. M., Huber, C., Türeci, Ö., & Sahin, U. (2010) · Cancer Research · doi:10.1158/0008-5472.can-10-0699 · Cited by 309 · see citing works
Cell specific delivery of modified mRNA expressing therapeutic proteins to leukocytes
Veiga, N., Goldsmith, M., Granot, Y., Rosenblum, D., Dammes, N., Kedmi, R., Ramishetti, S., & Peer, D. (2018) · Nature Communications · doi:10.1038/s41467-018-06936-1 · Cited by 308 · see citing works
A comparison of gene transfer methods in human dendritic cells.
Arthur, J. F., Butterfield, L. H., Roth, M. D., Bùi, L. A., Kiertscher, S. M., Lau, R., Dubinett, S., Glaspy, J. A., McBride, W. H., & Economou, J. S. (1997) · PubMed · Cited by 305 · see citing works
Fenton, O. S., Kauffman, K. J., Kaczmarek, J. C., McClellan, R. L., Jhunjhunwala, S., Tibbitt, M. W., Zeng, M. D., Appel, E. A., Dorkin, J. R., Mir, F. F., et al. (2017) · Advanced Materials · doi:10.1002/adma.201606944 · Cited by 289 · see citing works
Su, X., Fricke, J., Kavanagh, D. G., & Irvine, D. J. (2011) · Molecular Pharmaceutics · doi:10.1021/mp100390w · Cited by 287 · see citing works
Novel “anti-reverse” cap analogs with superior translational properties
Jemielity, J., Fowler, T. E., Zuberek, J., Stȩpiński, J., Lewdorowicz, M., Niedźwiecka-Kornaś, A., Stolarski, R., Darżynkiewicz, E., & Rhoads, R. E. (2003) · RNA · doi:10.1261/rna.5430403 · Cited by 263 · see citing works
Vaidyanathan, S., Azizian, K. T., Haque, A. K. M. A., Henderson, J. M., Hendel, A., Shore, S., Antony, J. S., Hogrefe, R. I., Kormann, M. S. D., Porteus, M. H., et al. (2018) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2018.06.010 · Cited by 262 · see citing works
Results of the First Phase I/II Clinical Vaccination Trial With Direct Injection of mRNA
Weide, B., Carralot, J.-P., Reese, A., Scheel, B., Eigentler, T. K., Hoerr, I., Rammensee, H.-G., Garbe, C., & Pascolo, S. (2008) · Journal of Immunotherapy · doi:10.1097/cji.0b013e31815ce501 · Cited by 261 · see citing works
Bioinspired Alkenyl Amino Alcohol Ionizable Lipid Materials for Highly Potent In Vivo mRNA Delivery
Fenton, O. S., Kauffman, K. J., McClellan, R. L., Appel, E. A., Dorkin, J. R., Tibbitt, M. W., Heartlein, M. W., DeRosa, F., Langer, R., & Anderson, D. G. (2016) · Advanced Materials · doi:10.1002/adma.201505822 · Cited by 254 · see citing works
Pollard, C., Rejman, J., De Haes, W., Verrier, B., Van Gulck, E., Naessens, T., De Smedt, S., Bogaert, P., Grooten, J., Vanham, G., et al. (2013) · Molecular Therapy · doi:10.1038/mt.2012.202 · Cited by 248 · see citing works
Regulatory Framework for Advanced Therapy Medicinal Products in Europe and United States
Iglesias-López, C., Agustí, A., Obach, M., & Vallano, A. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.00921 · Cited by 242 · see citing works
McKinlay, C. J., Benner, N. L., Haabeth, O. A. W., Waymouth, R. M., & Wender, P. A. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1805358115 · Cited by 240 · see citing works
Mechanism of action of mRNA-based vaccines
Iavarone, C., O’hagan, D. T., Yu, D., Delahaye, N. F., & Ulmer, J. B. (2017) · Expert Review of Vaccines · doi:10.1080/14760584.2017.1355245 · Cited by 234 · see citing works
mRNA as gene therapeutic: How to control protein expression
Tavernier, G., Andries, O., Demeester, J., Sanders, N. N., De Smedt, S. C., & Rejman, J. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2010.10.020 · Cited by 232 · see citing works
Cationic lipids enhance siRNA-mediated interferon response in mice
Ma, Z., Li, J., He, F., Wilson, A. S., Pitt, B. R., & Li, S. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.03.041 · Cited by 225 · see citing works
RNA-Based Vaccines in Cancer Immunotherapy
McNamara, M. A., Nair, S. K., & Holl, E. K. (2015) · Journal of Immunology Research · doi:10.1155/2015/794528 · Cited by 223 · see citing works
Increased Antigen Presentation Efficiency by Coupling Antigens to MHC Class I Trafficking Signals
Kreiter, S., Selmi, A., Diken, M., Sebastian, M., Osterloh, P., Schild, H., Huber, C., Türeci, Ö., & Şahin, U. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.180.1.309 · Cited by 221 · see citing works
mRNA Delivery for Therapeutic Anti-HER2 Antibody Expression In Vivo
Rybakova, Y., Kowalski, P. S., Huang, Y., Gonzalez, J. T., Heartlein, M. W., DeRosa, F., Delcassian, D., & Anderson, D. G. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.05.012 · Cited by 218 · see citing works
Preclinical Evaluation of TriMix and Antigen mRNA-Based Antitumor Therapy
Lint, S. V., Goyvaerts, C., Maenhout, S. K., Goethals, L., Disy, A., Benteyn, D., Pen, J. J., Bonehill, A., Heirman, C., Breckpot, K., et al. (2012) · Cancer Research · doi:10.1158/0008-5472.can-11-2957 · Cited by 210 · see citing works
Lipid-based mRNA vaccine delivery systems
Midoux, P., & Pichon, C. (2014) · Expert Review of Vaccines · doi:10.1586/14760584.2015.986104 · Cited by 209 · see citing works
Intradermal delivery of modified mRNA encoding VEGF-A in patients with type 2 diabetes
Gan, L., Fermer, M. L., Carlsson, L. G., Arfvidsson, C., Egnell, A., Rudvik, A., Kjaer, M., Collén, A., Thompson, J. D., Joyal, J. L., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-08852-4 · Cited by 209 · see citing works
Probst, J., Weide, B., Scheel, B., Pichler, B. J., Hoerr, I., Rammensee, H.-G., & Pascolo, S. (2007) · Gene Therapy · doi:10.1038/sj.gt.3302964 · Cited by 206 · see citing works
How mRNA therapeutics are entering the monoclonal antibody field
Van Hoecke, L., & Roose, K. (2019) · Journal of Translational Medicine · doi:10.1186/s12967-019-1804-8 · Cited by 205 · see citing works
In Vitro Transcription of Long RNA Containing Modified Nucleosides
Pardi, N., Muramatsu, H., Weissman, D., & Karikó, K. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-260-5_2 · Cited by 204 · see citing works
Viger-Gravel, J., Schantz, A., Pinon, A. C., Rossini, A. J., Schantz, S., & Emsley, L. (2018) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.7b10795 · Cited by 202 · see citing works
Comparison of DNA and mRNA vaccines against cancer
Jahanafrooz, Z., Baradaran, B., Mosafer, J., Hashemzaei, M., Rezaei, T., Mokhtarzadeh, A., & Hamblin, M. R. (2019) · Drug Discovery Today · doi:10.1016/j.drudis.2019.12.003 · Cited by 202 · see citing works
The Role of the Helper Lipid on the DNA Transfection Efficiency of Lipopolyplex Formulations
Du, Z., Munye, M. M., Tagalakis, A. D., Manunta, M., & Hart, S. L. (2014) · Scientific Reports · doi:10.1038/srep07107 · Cited by 200 · see citing works
Sedic, M., Senn, J. J., Lynn, A., Laska, M., Smith, M., Platz, S. J., Bolen, J., Hoge, S., Bulychev, A., Jacquinet, E., et al. (2018) · Veterinary Pathology · doi:10.1177/0300985817738095 · Cited by 195 · see citing works
Current prospects for mRNA gene delivery
Yamamoto, A., Kormann, M., Rosenecker, J., & Rudolph, C. (2008) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2008.09.016 · Cited by 193 · see citing works
Dendritic Cell-Targeted Vaccines
Cohn, L. B., & Delamarre, L. (2014) · Frontiers in Immunology · doi:10.3389/fimmu.2014.00255 · Cited by 191 · see citing works
Wang, Y.-H., Su, H.-H., Yang, Y., Hu, Y.-X., Zhang, L., Blancafort, P., & Huang, L. (2013) · Molecular Therapy · doi:10.1038/mt.2012.250 · Cited by 189 · see citing works
Lipopolyplex potentiates anti-tumor immunity of mRNA-based vaccination
Persano, S., Guevara, M. L., Li, Z., Mai, J., Ferrari, M., Pompa, P. P., & Shen, H. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.02.019 · Cited by 187 · see citing works
HPLC purification of in vitro transcribed long RNA
Weissman, D., Pardi, N., Muramatsu, H., & Karikó, K. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-260-5_3 · Cited by 180 · see citing works
Nucleic acid vaccines: prospects for non-viral delivery of mRNA vaccines
Deering, R. P., Kommareddy, S., Ulmer, J. B., Brito, L. A., & Geall, A. J. (2014) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2014.901308 · Cited by 177 · see citing works
Biomaterials for mRNA delivery
Islam, M. A., Reesor, E. K. G., Xu, Y., Zope, H. R., Zetter, B. R., & Shi, J. (2015) · Biomaterials Science · doi:10.1039/c5bm00198f · Cited by 176 · see citing works
Udhayakumar, V. K., De Beuckelaer, A., McCaffrey, J., McCrudden, C. M., Kirschman, J. L., Vanover, D., Van Hoecke, L., Roose, K., Deswarte, K., De Geest, B. G., et al. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201601412 · Cited by 169 · cited 8× · see citing works
“Similarly, the CPP RALA has been used to effectively deliver both eGFP and OVA mRNA and has been demonstrated to outperform the cationic lipid DOTAP and the fusogenic lipid DOPE [165].”
MethodInfluentialPhua, K. K. L., Leong, K. W., & Nair, S. K. (2013) · Journal of Controlled Release · doi:10.1016/j.jconrel.2012.12.029 · Cited by 159 · see citing works
Granot-Matok, Y., & Peer, D. (2017) · Seminars in Immunology · doi:10.1016/j.smim.2017.08.015 · Cited by 154 · see citing works
Lorenz, C., Fotin‐Mleczek, M., Roth, G. D., Becker, C., Dam, T. C., Verdurmen, W. P. R., Brock, R. E., Probst, J., & Schlake, T. (2011) · RNA Biology · doi:10.4161/rna.8.4.15394 · Cited by 152 · see citing works
Phosphorothioate cap analogs stabilize mRNA and increase translational efficiency in mammalian cells
Grudzien‐Nogalska, E., Jemielity, J., Kowalska, J., Darżynkiewicz, E., & Rhoads, R. E. (2007) · RNA · doi:10.1261/rna.701307 · Cited by 152 · see citing works
Nakanishi, M., & Otsu, M. (2012) · Current Gene Therapy · doi:10.2174/156652312802762518 · Cited by 147 · see citing works
Li, Y., & Kiledjian, M. (2010) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.15 · Cited by 145 · see citing works
mRNA Poly (A) Tail, a 3′ Enhancer of Translational Initiation
Munroe, D. J., & Jacobson, A. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.7.3441-3455.1990 · Cited by 138 · see citing works
New insights into decapping enzymes and selective mRNA decay
Grudzien‐Nogalska, E., & Kiledjian, M. (2016) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.1379 · Cited by 137 · see citing works
Structural and molecular mechanisms for the control of eukaryotic 5′–3′ mRNA decay
Mugridge, J. S., Coller, J., & Gross, J. D. (2018) · Nature Structural & Molecular Biology · doi:10.1038/s41594-018-0164-z · Cited by 133 · see citing works
Induction of HIV-1 gag specific immune responses by cationic micelles mediated delivery of gag mRNA
Zhao, M., Li, M., Zhang, Z., Gong, T., & Sun, X. (2016) · Drug Delivery · doi:10.3109/10717544.2015.1038856 · Cited by 132 · see citing works
mRNA as novel technology for passive immunotherapy
Schlake, T., Theß, A., Thran, M., & Jordan, I. (2018) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-018-2935-4 · Cited by 132 · see citing works
Physical methods of nucleic acid transfer: general concepts and applications
Julien Villemejane, A., & Mir, L. M. (2009) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2009.00032.x · Cited by 127 · see citing works
Poly(A) binding proteins: are they all created equal?
Goss, D. J., & Kleiman, F. E. (2013) · WIREs RNA · doi:10.1002/wrna.1151 · Cited by 127 · see citing works
Multifunctional triblock copolymers for intracellular messenger RNA delivery
Cheng, C., Convertine, A. J., Stayton, P. S., & Bryers, J. D. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.06.020 · Cited by 123 · see citing works
Chong, R. H. E., González-González, E., Lara, M. F., Speaker, T. J., Contag, C. H., Kaspar, R. L., Coulman, S. A., Hargest, R., & Birchall, J. C. (2013) · Journal of Controlled Release · doi:10.1016/j.jconrel.2012.12.030 · Cited by 122 · see citing works
Kallen, K., & Theß, A. (2013) · Therapeutic Advances in Vaccines · doi:10.1177/2051013613508729 · Cited by 120 · see citing works
mRNA therapeutics deliver a hopeful message
Zhong, Z., Cafferty, S. M., Combes, F., Huysmans, H., Temmerman, J. D., Gitsels, A., Vanrompay, D. C. G., Catani, J. P., & Sanders, N. N. (2018) · Nano Today · doi:10.1016/j.nantod.2018.10.005 · Cited by 120 · see citing works
Sun, N., Ning, B., Hansson, K. M., Bruce, A. C., Seaman, S. A., Zhang, C., Rikard, S. M., DeRosa, C. A., Fraser, C. L., Wågberg, M., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-35570-6 · Cited by 119 · see citing works
Dewitte, H., Lint, S. V., Heirman, C., Thielemans, K., Smedt, S. C. D., Breckpot, K., & Lentacker, I. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.08.011 · Cited by 118 · see citing works
Gary, D. J., Lee, H., Sharma, R., Lee, J., Kim, Y.-W., Cui, Z., Jia, D., Bowman, V. D., Chipman, P. R., Wan, L., et al. (2011) · ACS Nano · doi:10.1021/nn102540y · Cited by 117 · see citing works
Peptide-based gene delivery vectors
Kang, Z., Meng, Q., & Liu, K. (2019) · Journal of Materials Chemistry B · doi:10.1039/c8tb03124j · Cited by 117 · see citing works
Delivery of mRNA Therapeutics for the Treatment of Hepatic Diseases
Trepotec, Z., Lichtenegger, E., Plank, C., Aneja, M. K., & Rudolph, C. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2018.12.012 · Cited by 115 · see citing works
Optimizing Cardiac Delivery of Modified mRNA
Sultana, N., Magadum, A., Hadas, Y., Kondrat, J., Singh, N., Youssef, E., Calderon, D., Chepurko, E., Dubois, N. C., Hajjar, R. J., et al. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2017.03.016 · Cited by 114 · see citing works
Evading innate immunity in nonviral mRNA delivery: don't shoot the messenger.
Devoldere, J., Dewitte, H., De Smedt, S. C., & Remaut, K. (2016) · Drug Discovery Today · doi:10.1016/j.drudis.2015.07.009 · Cited by 112 · see citing works
Muttach, F., Muthmann, N., & Rentmeister, A. (2017) · Beilstein Journal of Organic Chemistry · doi:10.3762/bjoc.13.274 · Cited by 112 · see citing works
Poly(glycoamidoamine) Brushes Formulated Nanomaterials for Systemic siRNA and mRNA Delivery in Vivo
Dong, Y., Dorkin, J. R., Wang, W., Chang, P., Webber, M. J., Tang, B. C., Yang, J., Ionita, I. A., Danino, D., DeRosa, F., et al. (2016) · Nano Letters · doi:10.1021/acs.nanolett.5b02428 · Cited by 111 · see citing works
Optimization of mRNA untranslated regions for improved expression of therapeutic mRNA
Asrani, K. H., Farelli, J. D., Stahley, M. R., Miller, R. L., Cheng, C. J., Subramanian, R. R., & Brown, J. M. (2018) · RNA Biology · doi:10.1080/15476286.2018.1450054 · Cited by 111 · see citing works
Type I Interferons Modulate CD8 + T Cell Immunity to mRNA Vaccines
Beuckelaer, A. D., Grooten, J., & Koker, S. D. (2017) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2017.01.006 · Cited by 109 · see citing works
Kigasawa, K., Kajimoto, K., Hama, S., Saito, A., Kanamura, K., & Kogure, K. (2009) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2009.08.036 · Cited by 106 · see citing works
Koh, K. J., Liu, Y., Lim, S. H., Loh, X. J., Kang, L., Lim, C. Y., & Phua, K. K. L. (2018) · Scientific Reports · doi:10.1038/s41598-018-30290-3 · Cited by 105 · see citing works
Yu, H., Guo, C., Feng, B., Liu, J., Chen, X., Wang, D., Teng, L., Li, Y., Yin, Q., Zhang, Z., et al. (2015) · Theranostics · doi:10.7150/thno.13515 · Cited by 100 · see citing works
Zakrewsky, M., Kumar, S., & Mitragotri, S. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.09.017 · Cited by 99 · see citing works
Sunshine, J. C., Sunshine, S. B., Bhutto, I. A., Handa, J. T., & Green, J. J. (2012) · PLoS ONE · doi:10.1371/journal.pone.0037543 · Cited by 95 · see citing works
Wojtczak, B. A., Sikorski, P. J., Fac-Dabrowska, K., Nowicka, A. M., Warmiński, M., Kubacka, D., Nowak, E., Nowotny, M., Kowalska, J., & Jemielity, J. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.8b02597 · Cited by 95 · see citing works
Use of Dendritic Cell Receptors as Targets for Enhancing Anti-Cancer Immune Responses
Hossain, M. K., & Wall, K. A. (2019) · Cancers · doi:10.3390/cancers11030418 · Cited by 94 · see citing works
Vanlandingham, D. L., Tsetsarkin, K. A., Hong, C., Klingler, K. A., McElroy, K. L., Lehane, M. J., & Higgs, S. (2005) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2005.05.008 · Cited by 91 · see citing works
Kore, A. R., Shanmugasundaram, M., Charles, I., Vlassov, A. V., & Barta, T. J. (2009) · Journal of the American Chemical Society · doi:10.1021/ja901655p · Cited by 90 · see citing works
Selmi, A., Vascotto, F., Kautz‐Neu, K., Türeci, Ö., Şahin, U., von Stebut, E., Diken, M., & Kreiter, S. (2016) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-016-1869-7 · Cited by 90 · see citing works
Lu, Y. Q., Kawakami, S., Yamashita, F., & Hashida, M. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.03.028 · Cited by 88 · see citing works
Golombek, S., Pilz, M., Steinle, H., Kochba, E., Levin, Y., Lunter, D., Schlensak, C., Wendel, H. P., & Avci-Adali, M. (2018) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2018.03.005 · Cited by 88 · see citing works
Peptide-mediated delivery of therapeutic mRNA in ovarian cancer
van den Brand, D., Gorris, M. A. J., van Asbeck, A. H., Palmen, E., Ebisch, I. M. W., Dolstra, H., Hällbrink, M., Massuger, L. F. A. G., & Brock, R. E. (2019) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2019.05.014 · Cited by 88 · see citing works
Topical Delivery of Anti-sense Oligonucleotides Using Low-Frequency Sonophoresis
Tezel, A., Dokka, S., Kelly, S., Hardee, G. E., & Mitragotri, S. (2004) · Pharmaceutical Research · doi:10.1007/s11095-004-7674-6 · Cited by 87 · see citing works
Mechanistic profiling of the siRNA delivery dynamics of lipid–polymer hybrid nanoparticles
Colombo, S., Cun, D., Remaut, K., Bunker, M., Zhang, J., Martin-Bertelsen, B., Yaghmur, A., Braeckmans, K., Nielsen, H. M., & Foged, C. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.12.026 · Cited by 85 · see citing works
Self-citationLipid Polymer Hybrid Nanomaterials for mRNA Delivery
Zhao, W., Zhang, C., Li, B., Zhang, X., Luo, X. L., Zeng, C., Li, W., Gao, M., & Dong, Y. (2018) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-018-0536-9 · Cited by 85 · see citing works
Development of an optimized RNA-based murine norovirus reverse genetics system
Yunus, M. A., Chung, L. M. W., Chaudhry, Y., Bailey, D., & Goodfellow, I. (2010) · Journal of Virological Methods · doi:10.1016/j.jviromet.2010.07.006 · Cited by 80 · see citing works
Viral and Synthetic RNA Vector Technologies and Applications
Schott, J. W., Morgan, M., Galla, M., & Schambach, A. (2016) · Molecular Therapy · doi:10.1038/mt.2016.143 · Cited by 76 · see citing works
RNA and DNA interactions with zwitterionic and charged lipid membranes — A DSC and QCM-D study
Michanek, A., Kristen, N., Höök, F., Nylander, T., & Sparr, E. (2009) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2009.12.009 · Cited by 74 · see citing works
Recent advances in polymeric materials for the delivery of RNA therapeutics
Ulkoski, D., Bak, A., Wilson, J. T., & Krishnamurthy, V. R. (2019) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2019.1663822 · Cited by 74 · see citing works
RNA Viruses as Tools in Gene Therapy and Vaccine Development
Lundstrom, K. H. (2019) · Genes · doi:10.3390/genes10030189 · Cited by 73 · see citing works
Zohra, F. T., Chowdhury, E. H., Tada, S., Hoshiba, T., & Akaike, T. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.04.059 · Cited by 71 · see citing works
Thanki, K., Zeng, X., Justesen, S., Tejlmann, S., Falkenberg, E., Driessche, E. V., Nielsen, H. M., Franzyk, H., & Foged, C. (2017) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2017.07.014 · Cited by 71 · see citing works
Self-citationMessenger RNA (mRNA) nanoparticle tumour vaccination
Phua, K. K. L., Nair, S. K., & Leong, K. W. (2014) · Nanoscale · doi:10.1039/c4nr01346h · Cited by 70 · see citing works
Alphavirus vectors for vaccine production and gene therapy
Lundstrom, K. H. (2003) · Expert Review of Vaccines · doi:10.1586/14760584.2.3.445 · Cited by 67 · see citing works
Bragazzi, N. L., Gianfredi, V., Villarini, M., Rosselli, R., Nasr, A., Hussein, A., Martini, M., & Behzadifar, M. (2018) · Frontiers in Public Health · doi:10.3389/fpubh.2018.00062 · Cited by 67 · see citing works
Self-Replicating RNA Viruses for RNA Therapeutics
Lundstrom, K. H. (2018) · Molecules · doi:10.3390/molecules23123310 · Cited by 65 · see citing works
Jansen, M. A. A., Klausen, L. H., Thanki, K., Lyngsø, J., Pedersen, J. S., Franzyk, H., Nielsen, H. M., van Eden, W., Dong, M., Broere, F., et al. (2019) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2019.06.009 · Cited by 65 · see citing works
Self-citationToxicity profiling of several common RNAi-based nanomedicines: a comparative study
Landesman‐Milo, D., & Peer, D. (2013) · Drug Delivery and Translational Research · doi:10.1007/s13346-013-0158-7 · Cited by 64 · see citing works
Investigation of charge ratio variation in mRNA – DEAE-dextran polyplex delivery systems
Siewert, C., Haas, H., Nawroth, T., Ziller, A., Nogueira, S. S., Schroer, M. A., Blanchet, C. E., Svergun, D. I., Rădulescu, A., Bates, F., et al. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.10.020 · Cited by 63 · see citing works
Picornavirus Modification of a Host mRNA Decay Protein
Rozovics, J. M., Chase, A. J., Cathcart, A. L., Chou, W., Gershon, P. D., Palusa, S. G., Wilusz, J., & Semler, B. L. (2012) · mBio · doi:10.1128/mbio.00431-12 · Cited by 61 · see citing works
Wang, F., Xiao, W., Elbahnasawy, M. A., Bao, X., Zheng, Q., Gong, L., Zhou, Y., Yang, S., Fang, A., Farag, M. M. S., et al. (2018) · Frontiers in Pharmacology · doi:10.3389/fphar.2018.00980 · Cited by 60 · see citing works
Regulation of protein synthesis and the role of eIF3 in cancer
Hershey, J. W. B. (2010) · Brazilian Journal of Medical and Biological Research · doi:10.1590/s0100-879x2010007500098 · Cited by 59 · see citing works
Chandler, M. R., Johnson, M. B., Panigaj, M., & Afonin, K. A. (2019) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2019.10.011 · Cited by 59 · see citing works
Pärnaste, L., Arukuusk, P., Langel, K., Tenson, T., & Langel, Ü. (2017) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2017.02.003 · Cited by 58 · see citing works
Enhanced Delivery and Potency of Self-Amplifying mRNA Vaccines by Electroporation in Situ
Cu, Y., Broderick, K. E., Banerjee, K., Hickman, J., Otten, G. R., Barnett, S. W., Kichaev, G., Sardesai, N. Y., Ulmer, J. B., & Geall, A. J. (2013) · Vaccines · doi:10.3390/vaccines1030367 · Cited by 57 · see citing works
Modified ARCA analogs providing enhanced translational properties of capped mRNAs
Kocmik, I., Piecyk, K., Rudzińska, M., Niedźwiecka-Kornaś, A., Darżynkiewicz, E., Grzela, R., & Jankowska‐Anyszka, M. (2018) · Cell Cycle · doi:10.1080/15384101.2018.1486164 · Cited by 57 · see citing works
Kowalska, J., del Nogal, A. W., Darżynkiewicz, Z. M., Buck, J., Nicola, C., Kuhn, A. N., Łukaszewicz, M., Zuberek, J., Strenkowska, M., Ziemniak, M., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku757 · Cited by 56 · see citing works
Delivery of a Modified mRNA Encoding IL-22 Binding Protein (IL-22BP) for Colon Cancer Gene Therapy
Zhang, R., Men, K., Zhang, X., Huang, R., Tian, Y., Zhou, B., Yu, C., Wang, Y., Xiao, J., Hu, Q., et al. (2018) · Journal of Biomedical Nanotechnology · doi:10.1166/jbn.2018.2577 · Cited by 56 · see citing works
Creusot, R. J., Chang, P. P., Healey, D. G., Tcherepanova, I. Y., Nicolette, C. A., & Fathman, C. G. (2010) · Molecular Therapy · doi:10.1038/mt.2010.146 · Cited by 52 · see citing works
Michel, T., Luft, D., Abraham, M.-K., Reinhardt, S., Medina, M. L. S., Kurz, J., Schaller, M., Avci‐Adali, M., Schlensak, C., Peter, K., et al. (2017) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2017.07.013 · Cited by 50 · see citing works
de Groot, A. M., Thanki, K., Gangloff, M., Falkenberg, E., Zeng, X., Bijnen, D. C. J. V., van Eden, W., Franzyk, H., Nielsen, H. M., Broere, F., et al. (2018) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2018.02.003 · Cited by 42 · see citing works
Self-citationAlphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons
Ehrengruber, M. U., Schlesinger, S., & Lundstrom, K. H. (2011) · Current Protocols in Neuroscience · doi:10.1002/0471142301.ns0422s57 · Cited by 40 · see citing works
Polylactide-Based Reactive Micelles as a Robust Platform for mRNA Delivery
Lacroix, C., Humanes, A., Coiffier, C., Gigmès, D., Verrier, B., & Trimaille, T. (2020) · Pharmaceutical Research · doi:10.1007/s11095-019-2749-6 · Cited by 39 · see citing works
Suknuntha, K., Tao, L., Brok-Volchanskaya, V., D’Souza, S. S., Kumar, A., & Slukvin, I. (2018) · Stem Cell Reviews and Reports · doi:10.1007/s12015-018-9805-1 · Cited by 35 · see citing works
Myc Regulation of mRNA Cap Methylation
Cowling, V. H., & Cole, M. D. (2010) · Genes & Cancer · doi:10.1177/1947601910378025 · Cited by 34 · see citing works
mRNA Transfection of Mouse and Human Neural Stem Cell Cultures
McLenachan, S., Zhang, D., Palomo, A. B. A., Edel, M. J., & Chen, F. K. (2013) · PLoS ONE · doi:10.1371/journal.pone.0083596 · Cited by 33 · see citing works
Zimmermann, O., Homann, J. M., Bangert, A., Müller, A., Hristov, G., Göser, S., Wiehe, J. M., Zittrich, S., Rottbauer, W., Torzewski, J., et al. (2012) · Journal of the American Heart Association · doi:10.1161/jaha.112.003293 · Cited by 33 · see citing works
Interaction of Poliovirus-Specific RNAs with HeLa Cells and E. coli
Koch, G. (1973) · Modern aspects of electrochemistry · doi:10.1007/978-3-642-65772-6_4 · Cited by 32 · see citing works
Rydzik, A. M., Warmiński, M., Sikorski, P. J., Baranowski, M. R., Walczak, S., Kowalska, J., Zuberek, J., Łukaszewicz, M., Nowak, E., Claridge, T. D. W., et al. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx569 · Cited by 32 · see citing works
In Vitro-Transcribed (IVT)-mRNA CAR Therapy Development
Miliotou, Α. N., & Papadopoulou, L. C. (2019) · Methods in molecular biology · doi:10.1007/978-1-0716-0146-4_7 · Cited by 32 · see citing works
Hecker, J. G. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-3271-9_23 · Cited by 29 · see citing works
Bounds, C. E., Terry, F. E., Moise, L., Hannaman, D., Martin, W. D., Groot, A. S. D., Suschak, J. J., Dupuy, L. C., & Schmaljohn, C. S. (2017) · Human Vaccines & Immunotherapeutics · doi:10.1080/21645515.2017.1329788 · Cited by 28 · see citing works
Synergistic Transdermal Delivery of Biomacromolecules Using Sonophoresis after Microneedle Treatment
Ryu, Y. C., Kim, D. I., Kim, S. H., Wang, H., & Hwang, B. H. (2018) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-018-0070-6 · Cited by 28 · see citing works
Recombinant AAV-directed gene therapy for type I glycogen storage diseases
CHOU, J. Y., & Mansfield, B. C. (2011) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2011.578067 · Cited by 27 · see citing works
Walther, W., Stein, U., Fichtner, I., Kobelt, D., Aumann, J., Arlt, F., & Schlag, P. M. (2005) · Molecular Therapy · doi:10.1016/j.ymthe.2005.07.700 · Cited by 26 · see citing works
Synthetic Messenger RNA and Cell Metabolism Modulation
Rabinovich, P. M. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-260-5 · Cited by 26 · see citing works
Shanmugasundaram, M., Charles, I., & Kore, A. R. (2016) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2016.01.048 · Cited by 24 · see citing works
Effect of locally administered Syk siRNA on allergen-induced arthritis and asthma
Huang, Z.-Y., Kim, M.-K., Kim-Han, T.-H., Indik, Z. K., & Schreiber, A. D. (2012) · Molecular Immunology · doi:10.1016/j.molimm.2012.06.011 · Cited by 23 · see citing works
Zhao, Y., Piao, Y. Z., Zhang, C., Jiang, Y., Liu, A., Cui, S., Zhi, D., Zhen, Y., & Zhang, S. (2017) · Journal of Materials Chemistry B · doi:10.1039/c7tb01915g · Cited by 21 · see citing works
A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells
Takahashi, M., Elbarbary, R. A., Nakashima, A., Abé, M., Watanabe, N., Narita, M., Takahashi, M., Tamura, M., Yoshida, T., & Nashimoto, M. (2012) · Cancer Letters · doi:10.1016/j.canlet.2012.10.016 · Cited by 20 · see citing works
Adibzadeh, S., Fardaei, M., Takhshid, M. A., Miri, M. R., Dehbidi, G. R., Farhadi, A., Ranjbaran, R., Alavi, P., Nikouyan, N., Seyyedi, N., et al. (2019) · Europe PMC (PubMed Central) · Cited by 20 · see citing works
MAR characteristic motifs mediate episomal vector in CHO cells.
Lin, Y., Li, Z., Wang, T.-Y., Wang, X.-Y., Wang, L., Dong, W.-H., Jing, C.-Q., & Yang, X. (2015) · Gene · doi:10.1016/j.gene.2015.01.032 · Cited by 19 · see citing works
Taylor, K., Kolokoltsova, O. A., Ronca, S. E., Estes, M., & Paessler, S. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00081 · Cited by 19 · see citing works
Phosphate-modified analogues of m7GTP and m7Gppppm7G—Synthesis and biochemical properties
Ziemniak, M., Kowalska, J., Łukaszewicz, M., Zuberek, J., Wnek, K., Darżynkiewicz, E., & Jemielity, J. (2015) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2015.07.052 · Cited by 17 · see citing works
Viral Hybrid-Vectors for Delivery of Autonomous Replicons
Zhang, W., Hagedorn, C., Ehrke‐Schulz, E., Lipps, H., & Ehrhardt, A. (2014) · Current Gene Therapy · doi:10.2174/1566523213666131223130024 · Cited by 17 · see citing works
Bhattacharya, N., Ganguli‐Indra, G., & Indra, A. K. (2019) · Expert Review of Proteomics · doi:10.1080/14789450.2019.1646128 · Cited by 12 · see citing works
Alphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons
Ehrengruber, M. U., & Lundstrom, K. H. (2007) · Current Protocols in Neuroscience · doi:10.1002/0471142301.ns0422s41 · Cited by 12 · see citing works
Ahlemeyer, B., Vogt, J.-F., Michel, V., Hahn-Kohlberger, P., & Baumgart‐Vogt, E. (2014) · Histochemistry and Cell Biology · doi:10.1007/s00418-014-1254-6 · Cited by 11 · see citing works
Polyethylenimine-based polyplex nanoparticles and features of their behavior in cells and tissues
Rosenkranz, A. A., & Sobolev, A. S. (2015) · Russian Chemical Bulletin · doi:10.1007/s11172-015-1220-z · Cited by 9 · see citing works
Hoerr, I. (2000) · doi:10.1002/1521-4141(200001)30:1
Flagged first, then by citations
2 of the 184 references couldn’t be matched to a known paper.
Research works printed but not linked: 2
- [54]Shin D. Azizian K.T. Henderson J.M. Lebedev A. Hogrefe R.I. Houston M. McCaffrey A.P. BioTechnologies T. Exploring the Messenger RNA Capping Code: CleanCap Co-Transcriptional Capping Allows Synthesis of Cap 0, Cap 1, Cap 2 and (M6) A (M) Capped RNAs Cell Press Cambridge, MA, USA 2018 91
- [81]Sahin U. Karikó K. Türeci Ö. mRNA-based therapeutics — developing a new class of drugs Nat. Pub. Gr. 2014 10.1038/nrd4278 25233993 · DOI · Paper page
Cited by
Sorted by citations
mRNA vaccines for infectious diseases: principles, delivery and clinical translation
Chaudhary, N., Weissman, D., & Whitehead, K. A. (2021) · Nature Reviews Drug Discovery · doi:10.1038/s41573-021-00283-5 · Cited by 1,555 · see citing works
The clinical progress of mRNA vaccines and immunotherapies
Barbier, A. J., Jiang, A. Y., Zhang, P., Wooster, R., & Anderson, D. G. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01294-2 · Cited by 823 · see citing works
mRNA-based therapeutics: powerful and versatile tools to combat diseases
Qin, S., Tang, X., Chen, Y., Chen, K., Fan, N., Xiao, W., Zheng, Q., Li, G., Teng, Y., Wu, M., et al. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01007-w · Cited by 801 · see citing works
Advances in COVID-19 mRNA vaccine development
Fang, E., Liu, X., Li, M., Zhang, Z., Song, L., Zhu, B., Wu, X., Liu, J., Zhao, D., & Li, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00950-y · Cited by 601 · see citing works
Endosomal escape: A bottleneck for LNP-mediated therapeutics
Chatterjee, S., Kon, E., Sharma, P., & Peer, D. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2307800120 · Cited by 607 · see citing works
Lipid nanoparticles for nucleic acid delivery: Current perspectives
Samaridou, E., Heyes, J. A., & Lutwyche, P. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.06.002 · Cited by 593 · see citing works
Nanomaterial Delivery Systems for mRNA Vaccines
Buschmann, M. D., Carrasco, M. J., Alishetty, S., Paige, M., Alameh, M. G., & Weissman, D. (2021) · Vaccines · doi:10.3390/vaccines9010065 · Cited by 582 · see citing works
Self-amplifying RNA vaccines for infectious diseases
Bloom, K., van den Berg, F., & Arbuthnot, P. (2021) · Gene Therapy · doi:10.1038/s41434-020-00204-y · Cited by 487 · see citing works
Sebastiani, F., Yanez Arteta, M., Lerche, M., Porcar, L., Lang, C., Bragg, R. A., Elmore, C. S., Krishnamurthy, V. R., Russell, R. A., Darwish, T., et al. (2021) · ACS Nano · doi:10.1021/acsnano.0c10064 · Cited by 409 · see citing works
Challenges of Storage and Stability of mRNA-Based COVID-19 Vaccines
Uddin, M. N., & Roni, M. A. (2021) · Vaccines · doi:10.3390/vaccines9091033 · Cited by 371 · see citing works
mRNA Vaccine Era—Mechanisms, Drug Platform and Clinical Prospection
Xu, S., Yang, K., Li, R., & Zhang, L. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21186582 · Cited by 368 · see citing works
Nanotechnology in healthcare, and its safety and environmental risks
Ma, X., Tian, Y., Yang, R., Wang, H., Allahou, L. W., Chang, J., Williams, G. R., Knowles, J. C., & Poma, A. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02901-x · Cited by 362 · see citing works
mRNA vaccines for COVID-19: what, why and how
Park, J. W., Lagniton, P. N. P., Liu, Y., & Xu, R.-H. (2021) · International Journal of Biological Sciences · doi:10.7150/ijbs.59233 · Cited by 321 · see citing works
Recent advances in exosome-mediated nucleic acid delivery for cancer therapy
Zhang, Y., Liu, Q., Zhang, X., Huang, H., Tang, S., Chai, Y., Xu, Z., Li, M., Chen, X., Liu, J., et al. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01472-z · Cited by 306 · see citing works
The Importance of Apparent pKa in the Development of Nanoparticles Encapsulating siRNA and mRNA
Patel, P., Ibrahim, N. M., & Cheng, K. (2021) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2021.03.002 · Cited by 293 · see citing works
Role of nanotechnology behind the success of mRNA vaccines for COVID-19
Khurana, A., Allawadhi, P., Khurana, I., Allwadhi, S., Weiskirchen, R., Banothu, A. K., Chhabra, D., Joshi, K., & Bharani, K. K. (2021) · Nano Today · doi:10.1016/j.nantod.2021.101142 · Cited by 286 · see citing works
The Critical Contribution of Pseudouridine to mRNA COVID-19 Vaccines
Morais, P., Adachi, H., & Yu, Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.789427 · Cited by 274 · see citing works
Lipid Nanoparticle–mRNA Formulations for Therapeutic Applications
Wang, C., Zhang, Y., & Dong, Y. (2021) · Accounts of Chemical Research · doi:10.1021/acs.accounts.1c00550 · Cited by 270 · see citing works
From influenza to COVID-19: Lipid nanoparticle mRNA vaccines at the frontiers of infectious diseases
Pilkington, E. H., Suys, E. J. A., Trevaskis, N. L., Wheatley, A. K., Zukancic, D., Algarni, A., Al-Wassiti, H. A., Davis, T. P., Pouton, C. W., Kent, S. J., et al. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.06.023 · Cited by 265 · see citing works
Advances in Lipid Nanoparticles for mRNA-Based Cancer Immunotherapy
Guevara, M. L., Persano, F., & Persano, S. (2020) · Frontiers in Chemistry · doi:10.3389/fchem.2020.589959 · Cited by 257 · see citing works
mRNA vaccines in disease prevention and treatment
Zhang, G., Tang, T., Chen, Y., Huang, X., & Liang, T. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01579-1 · Cited by 253 · see citing works
Lipid Nanoparticles as Delivery Systems for RNA-Based Vaccines
Aldosari, B. N., Alfagih, I. M., & Almurshedi, A. S. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13020206 · Cited by 242 · see citing works
Jeong, M., Lee, Y., Park, J., Jung, H., & Lee, H. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114990 · Cited by 227 · see citing works
Vaccine delivery systems toward lymph nodes
Ding, Y., Li, Z., Jaklenec, A., & Hu, Q. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.113914 · Cited by 189 · see citing works
Exosomes as bio-inspired nanocarriers for RNA delivery: preparation and applications
Amiri, A., Bagherifar, R., Ansari Dezfouli, E., Kiaie, S. H., Jafari, R., & Ramezani, R. (2022) · Journal of Translational Medicine · doi:10.1186/s12967-022-03325-7 · Cited by 186 · see citing works
Souto, E. B., Blanco-Llamero, C., Krambeck, K., Kiran, N. S., Yashaswini, C., Postwala, H. I., Severino, P. B., Priefer, R., Prajapati, B. G., & Maheshwari, R. (2024) · Acta Biomaterialia · doi:10.1016/j.actbio.2024.04.010 · Cited by 182 · see citing works
Recent Advances in Lipid Nanoparticles for Delivery of mRNA
Yang, L., Gong, L., Wang, P., Zhao, X., Zhao, F. H., Zhang, Z., Li, Y., & Huang, W. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14122682 · Cited by 178 · see citing works
Recent Advancement in mRNA Vaccine Development and Applications
Fayez, N. A., Nassar, M. S., Alshehri, A. A., Alnefaie, M. K., Almughem, F. A., Alshehri, B. Y., Alawad, A. O., & Tawfik, E. A. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15071972 · Cited by 177 · see citing works
Factors Affecting Stability of RNA – Temperature, Length, Concentration, pH, and Buffering Species
Chheda, U., Pradeepan, S., Esposito, E. A., Strezsak, S. R., Fernandez‐Delgado, O., & Kranz, J. K. (2023) · Journal of Pharmaceutical Sciences · doi:10.1016/j.xphs.2023.11.023 · Cited by 166 · see citing works
Qin, F., Xia, F., Chen, H., Cui, B., Feng, Y., Zhang, P., Chen, J., & Luo, M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.633776 · Cited by 156 · see citing works
Comparison of DLin-MC3-DMA and ALC-0315 for siRNA Delivery to Hepatocytes and Hepatic Stellate Cells
Ferraresso, F., Strilchuk, A. W., Juang, L. J., Poole, L. G., Luyendyk, J. P., & Kastrup, C. J. (2022) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.2c00033 · Cited by 151 · see citing works
Therapeutic induction of antigen-specific immune tolerance
Kenison, J. E., Stevens, N. A., & Quintana, F. J. (2023) · Nature reviews. Immunology · doi:10.1038/s41577-023-00970-x · Cited by 154 · see citing works
Muskan, M., Abeysinghe, P., Cecchin, R., Branscome, H., Morris, K. V., & Kashanchi, F. (2024) · Molecular Therapy · doi:10.1016/j.ymthe.2024.02.025 · Cited by 142 · see citing works
Non-viral delivery of the CRISPR/Cas system: DNA versus RNA versus RNP
Lin, Y., Wagner, E., & Lächelt, U. (2022) · Biomaterials Science · doi:10.1039/d1bm01658j · Cited by 141 · see citing works
Nanomedicine-Based Approaches for mRNA Delivery
Uchida, S., Perche, F., Pichon, C., & Cabral, H. (2020) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.0c00618 · Cited by 139 · see citing works
Zhang, H., Vandesompele, J., Braeckmans, K., Smedt, S. C. D., & Remaut, K. (2023) · Chemical Society Reviews · doi:10.1039/d3cs00194f · Cited by 140 · see citing works
Antigen Presentation of mRNA-Based and Virus-Vectored SARS-CoV-2 Vaccines
Rijkers, G. T., Weterings, N., Obregón‐Henao, A., Dutt, T., Dutt, T. S., van Overveld, F. J., & Henao-Tamayo, M. I. (2021) · Vaccines · doi:10.3390/vaccines9080848 · Cited by 134 · see citing works
Game changers in science and technology - now and beyond
Betz, U. A. K., Arora, L., Assal, R. A., de Azevedo, H. F. Z., Baldwin, J., Becker, M. S., Bostock, S., Cheng, V. W. T., Egle, T., Ferrari, N., et al. (2023) · Technological Forecasting and Social Change · doi:10.1016/j.techfore.2023.122588 · Cited by 146 · see citing works
Nanoplatforms for mRNA Therapeutics
Meng, C., Chen, Z., Li, G., Welte, T., & Shen, H. (2021) · Advanced Therapeutics · doi:10.1002/adtp.202000099 · Cited by 130 · see citing works
Circular RNA: A promising new star of vaccine
Xie, J., Ye, F., Deng, X., Tang, Y., Liang, J., Huang, X., Sun, Y., Tang, H., Lei, J., Zheng, S., et al. (2023) · Journal of Translational Internal Medicine · doi:10.2478/jtim-2023-0122 · Cited by 127 · see citing works
Liposome-polymer complex for drug delivery system and vaccine stabilization
Sriwidodo, S., Umar, A. K., Wathoni, N., Zothantluanga, J. H., Das, S., & Luckanagul, J. A. (2022) · Heliyon · doi:10.1016/j.heliyon.2022.e08934 · Cited by 119 · see citing works
A perspective on oligonucleotide therapy: Approaches to patient customization
Thakur, S., Sinhari, A., Jain, P., & Jadhav, H. R. (2022) · Frontiers in Pharmacology · doi:10.3389/fphar.2022.1006304 · Cited by 114 · see citing works
From COVID-19 to Cancer mRNA Vaccines: Moving From Bench to Clinic in the Vaccine Landscape
Chakraborty, C., Sharma, A. R., Bhattacharya, M., & Lee, S. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.679344 · Cited by 111 · see citing works
Advances in mRNA non-viral delivery approaches
Ibba, M. L., Ciccone, G., Esposito, C. L., Catuogno, S., & Giangrande, P. H. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.113930 · Cited by 107 · see citing works
An overview of rational design of mRNA-based therapeutics and vaccines
To, K. K. W., & Cho, W. C. S. (2021) · Expert Opinion on Drug Discovery · doi:10.1080/17460441.2021.1935859 · Cited by 110 · see citing works
Mutations in SARS-CoV-2: Insights on structure, variants, vaccines, and biomedical interventions
Abulsoud, A. I., El‐Husseiny, H. M., El-Husseiny, A. A., El‐Mahdy, H. A., Ismail, A., Elkhawaga, S. Y., Khidr, E. G., Fathi, D., Mady, E. A., Najda, A., et al. (2022) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2022.113977 · Cited by 108 · see citing works
mRNA-Based Cancer Vaccines: A Therapeutic Strategy for the Treatment of Melanoma Patients
Bidram, M., Zhao, Y., Shebardina, N. G., Baldin, A. V., Bazhin, A. V., Ganjalikhany, M. R., Zamyatnin, A. A., & Ganjalıkhani-Hakemi, M. (2021) · Vaccines · doi:10.3390/vaccines9101060 · Cited by 106 · see citing works
The Progress and Promise of RNA Medicine─An Arsenal of Targeted Treatments
Sasso, J. M., Ambrose, B. J. B., Tenchov, R., Datta, R. S., Basel, M. T., DeLong, R. K., & Zhou, Q. A. (2022) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.2c00024 · Cited by 104 · see citing works
Engineering synthetic RNA devices for cell control
Dykstra, P. B., Kaplan, M., & Smolke, C. D. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-021-00436-7 · Cited by 111 · see citing works
Sung, J. J., Alghoul, Z., Long, D., Yang, C., & Merlin, D. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121707 · Cited by 103 · see citing works
Wu, Z., & Li, T. (2021) · Pharmaceutical Research · doi:10.1007/s11095-021-03015-x · Cited by 102 · see citing works
Review of structural design guiding the development of lipid nanoparticles for nucleic acid delivery
Cárdenas, M., Campbell, R. A., Arteta, M. Y., Lawrence, M. J., & Sebastiani, F. (2023) · Current Opinion in Colloid & Interface Science · doi:10.1016/j.cocis.2023.101705 · Cited by 101 · see citing works
Advances in gene-based vaccine platforms to address the COVID-19 pandemic
Pushparajah, D., Jimenez, S., Wong, S. L., Alattas, H., Nafissi, N., & Slavcev, R. A. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.01.003 · Cited by 98 · see citing works
New vaccine production platforms used in developing SARS-CoV-2 vaccine candidates
Ura, T., Yamashita, A., Mizuki, N., Okuda, K., & Shimada, M. (2020) · Vaccine · doi:10.1016/j.vaccine.2020.11.054 · Cited by 96 · see citing works
RNA therapeutics in the clinic
Curreri, A. M., Sankholkar, D., Mitragotri, S., & Zhao, Z. (2022) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10374 · Cited by 96 · see citing works
Gene therapy to enhance angiogenesis in chronic wounds
Shaabani, E., Sharifiaghdam, M., Majidi, R. F., Smedt, S. C. D., Braeckmans, K., & Fraire, J. C. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.08.020 · Cited by 99 · see citing works
Therapeutic cancer vaccines: From biological mechanisms and engineering to ongoing clinical trials
Sobhani, N., Scaggiante, B., Morris, R. M., Chai, D., Catalano, M., Tardiel-Cyril, D. R., Neeli, P. K., Roviello, G., Mondani, G., & Li, Y. (2022) · Cancer Treatment Reviews · doi:10.1016/j.ctrv.2022.102429 · Cited by 99 · see citing works
Zhang, C., Ma, Y., Zhang, J., Kuo, J. C., Zhang, Z., Xie, H., Zhu, J., & Liu, T. (2022) · Molecules · doi:10.3390/molecules27061943 · Cited by 93 · see citing works
New Advances in Biomedical Application of Polymeric Micelles
Figueiras, A., Domingues, C., Jarak, I., Santos, A. I., Parra, A., Pais, A. A. C. C., Alvarez‐Lorenzo, C., Concheiro, Á., Kabanov, A. V., Cabral, H., et al. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14081700 · Cited by 95 · see citing works
A predictive mechanistic model of drug release from surface eroding polymeric nanoparticles
Stiepel, R. T., Pena, E. S., Ehrenzeller, S. A., Gallovic, M. D., Lifshits, L. M., Genito, C. J., Bachelder, E. M., & Ainslie, K. M. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.09.067 · Cited by 94 · see citing works
Gómez, X., Sanon, S., Zambrano, K., Asquel, S., Bassantes, M., Morales, J. E., Otáñez, G., Pomaquero, C., Villarroel, S., Zurita, A., et al. (2021) · npj Microgravity · doi:10.1038/s41526-021-00162-8 · Cited by 92 · see citing works
Nucleic Acid Vaccines for COVID-19: A Paradigm Shift in the Vaccine Development Arena
Chavda, V. P., Hossain, M. M. M., Beladiya, J., & Apostolopoulos, V. (2021) · Biologics · doi:10.3390/biologics1030020 · Cited by 92 · see citing works
Pesticide pestilence: Global scenario and recent advances in detection and degradation methods
Raj, A., Dubey, A., Malla, M. A., & Kumar, A. (2023) · Journal of Environmental Management · doi:10.1016/j.jenvman.2023.117680 · Cited by 91 · see citing works
Synthetic modified messenger RNA for therapeutic applications
Gao, M., Zhang, Q., Feng, X.-H., & Liu, J. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.06.020 · Cited by 89 · see citing works
Rational design and combinatorial chemistry of ionizable lipids for RNA delivery
Xu, Y., Golubovic, A., Xu, S., Pan, A., & Li, B. (2023) · Journal of Materials Chemistry B · doi:10.1039/d3tb00649b · Cited by 84 · see citing works
A Systematic Review on COVID-19 Vaccine Strategies, Their Effectiveness, and Issues
Khandker, S. S., Godman, B., Jawad, M. I., Meghla, B. A., Tisha, T. A., Khondoker, M. U., Haq, M. A., Charan, J., Talukder, A. A., Azmuda, N., et al. (2021) · Vaccines · doi:10.3390/vaccines9121387 · Cited by 84 · see citing works
Nanoparticles for vaccine and gene therapy: Overcoming the barriers to nucleic acid delivery
Mollé, L. M., Smyth, C. H., Yuen, D., & Johnston, A. P. R. (2022) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.1809 · Cited by 86 · see citing works
Luo, M., Lee, L. K. C., Peng, B., Choi, C. H. J., Tong, W. Y., & Voelcker, N. H. (2022) · Advanced Science · doi:10.1002/advs.202201740 · Cited by 81 · see citing works
mRNA-Based Vaccines and Therapeutics for COVID-19 and Future Pandemics
Chavda, V. P., Soni, S., Vora, L. K., Soni, S., Khadela, A. D., & Ajabiya, J. (2022) · Vaccines · doi:10.3390/vaccines10122150 · Cited by 82 · see citing works
Zukancic, D., Suys, E. J. A., Pilkington, E. H., Algarni, A., Al-Wassiti, H. A., & Truong, N. P. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12111068 · Cited by 79 · see citing works
Nanoparticles as Adjuvants and Nanodelivery Systems for mRNA-Based Vaccines
Alfagih, I. M., Aldosari, B. N., AlQuadeib, B. T., Almurshedi, A. S., & Alfagih, M. M. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics13010045 · Cited by 79 · see citing works
An overview on inactivated and live‐attenuated SARS‐CoV‐2 vaccines
Khoshnood, S., Arshadi, M., Akrami, S., Koupaei, M., Ghahramanpour, H., Shariati, A., Sadeghifard, N., & Heidary, M. (2022) · Journal of Clinical Laboratory Analysis · doi:10.1002/jcla.24418 · Cited by 76 · see citing works
Recent Advances in Cancer Vaccines: Challenges, Achievements, and Futuristic Prospects
Gupta, M., Wahi, A., Sharma, P., Nagpal, R., Raina, N., Kaurav, M., Bhattacharya, J., Oliveira, S. M. R., Dolma, K. G., Paul, A. K., et al. (2022) · Vaccines · doi:10.3390/vaccines10122011 · Cited by 79 · see citing works
van de Berg, D., Kis, Z., Behmer, C. F., Samnuan, K., Blakney, A. K., Kontoravdi, C., Shattock, R. J., & Shah, N. (2021) · npj Vaccines · doi:10.1038/s41541-021-00322-7 · Cited by 75 · see citing works
Self-Amplifying RNA Approach for Protein Replacement Therapy
Papukashvili, D., Rcheulishvili, N., Liu, C., Ji, Y., He, Y., & Wang, P. G. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232112884 · Cited by 76 · see citing works
Microneedle-Based Vaccine Delivery: Review of an Emerging Technology
Mansoor, I., Eassa, H. A., Mohammed, K. H. A., El-Fattah, M. A. A., Abdo, M. H., Rashad, E., Eassa, H. A., Saleh, A., Amin, O. M., Nounou, M. I., et al. (2022) · AAPS PharmSciTech · doi:10.1208/s12249-022-02250-8 · Cited by 74 · see citing works
Current Status of COVID‐19 (Pre)Clinical Vaccine Development
Ye, T., Zhong, Z., García‐Sastre, A., Schotsaert, M., & Geest, B. G. D. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.202008319 · Cited by 73 · see citing works
Gene Editing by Extracellular Vesicles
Kostyushev, D. S., Kostyusheva, A., Brezgin, S., Vv, S., Volchkova, E. V., Lukashev, A. N., & Chulanov, V. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21197362 · Cited by 73 · see citing works
Lipid Nanoparticles as Promising Carriers for mRNA Vaccines for Viral Lung Infections
Asr, M. H., Dayani, F., Segherloo, F. S., Kamedi, A., Neill, A. O., MacLoughlin, R. J., & Doroudian, M. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15041127 · Cited by 73 · see citing works
mRNA – A game changer in regenerative medicine, cell-based therapy and reprogramming strategies
Chabanovska, O., Galow, A.-M., David, R., & Lemcke, H. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.114002 · Cited by 76 · see citing works
Nanomaterials for mRNA ‐based therapeutics: Challenges and opportunities
Li, D., Liu, Q., Yang, M., Xu, H., Zhu, M., Zhang, Y., Xu, J., Tian, C., Yao, J., Wang, L., et al. (2023) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10492 · Cited by 71 · see citing works
Mildner, R., Hak, S., Parot, J., Hyldbakk, A., Borgos, S. E., Some, D., Johann, C., & Caputo, F. (2021) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2021.03.004 · Cited by 71 · see citing works
mRNA Therapeutic Modalities Design, Formulation and Manufacturing under Pharma 4.0 Principles
Ouranidis, A., Vavilis, T., Mandala, E., Davidopoulou, C., Stamoula, Ε., Markopoulou, C. K., Karagianni, A., & Kachrimanis, K. (2021) · Biomedicines · doi:10.3390/biomedicines10010050 · Cited by 72 · see citing works
Zhang, Z., Shen, Q., & Chang, H. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.843928 · Cited by 69 · see citing works
The Delivery of mRNA Vaccines for Therapeutics
Nitika, Wei, J., & Hui, A. (2022) · Life · doi:10.3390/life12081254 · Cited by 70 · see citing works
Siddiqui, S. A., Bahmid, N. A., Taha, A., Abdel‐Moneim, A. E., Shehata, A. M., Tan, C., Kharazmi, M. S., Li, Y., Assadpour, E., Castro‐Muñoz, R., et al. (2022) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2022.102772 · Cited by 68 · see citing works
Optimization of DOTAP/chol Cationic Lipid Nanoparticles for mRNA, pDNA, and Oligonucleotide Delivery
Sun, M., Dang, U. J., Yuan, Y., Psaras, A. M., Osipitan, O. O., Brooks, T. A., Lu, F., & Pasqua, A. J. D. (2022) · AAPS PharmSciTech · doi:10.1208/s12249-022-02294-w · Cited by 66 · see citing works
Zhou, J., Ye, T., Yang, Y., Li, E., Zhang, K., Wang, Y., Chen, S., Hu, J., Zhang, K., Liu, F., et al. (2024) · Molecular Therapy · doi:10.1016/j.ymthe.2024.04.028 · Cited by 66 · see citing works
Delivery of mRNA for regulating functions of immune cells
Shi, J. Y., Huang, M., Lu, Z.-D., Du, X., Shen, S., Xu, C., & Wang, J. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.03.033 · Cited by 62 · see citing works
Marques, R., Lacerda, R., & Romão, L. (2022) · Biomedicines · doi:10.3390/biomedicines10081865 · Cited by 65 · see citing works
Neoantigen Vaccines; Clinical Trials, Classes, Indications, Adjuvants and Combinatorial Treatments
Niemi, J. V. L., Sokolov, A. V., & Schiöth, H. B. (2022) · Cancers · doi:10.3390/cancers14205163 · Cited by 63 · see citing works
Optimization of formulation and atomization of lipid nanoparticles for the inhalation of mRNA
Miao, H., Huang, K., Li, Y., Li, R., Zhou, X., Shi, J., Tong, Z., Sun, Z., & Yu, A. (2023) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2023.123050 · Cited by 64 · see citing works
Nanoparticle-based delivery strategies of multifaceted immunomodulatory RNA for cancer immunotherapy
Yoo, Y. J., Lee, C. H., Park, S. H., & Lim, Y. T. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.01.047 · Cited by 62 · see citing works
The impact of BNT162b2 mRNA vaccine on adaptive and innate immune responses
Föhse, K., Geckin, B., Zoodsma, M., Kılıç, G., Liu, Z., Röring, R. J., Overheul, G. J., van de Maat, J., Bulut, O. P., Hoogerwerf, J. J., et al. (2023) · Clinical Immunology · doi:10.1016/j.clim.2023.109762 · Cited by 62 · see citing works
Recent Advances in the Noninvasive Delivery of mRNA
Qin, M., Du, G., & Sun, X. (2021) · Accounts of Chemical Research · doi:10.1021/acs.accounts.1c00493 · Cited by 60 · see citing works
The paradigm shift in treatment from Covid-19 to oncology with mRNA vaccines
Wei, J., & Hui, A.-M. (2022) · Cancer Treatment Reviews · doi:10.1016/j.ctrv.2022.102405 · Cited by 57 · see citing works
Principles of lipid nanoparticle design for mRNA delivery
Zhang, Y., Zhang, X., Gao, Y., & Liu, S. (2024) · BMEMat · doi:10.1002/bmm2.12116 · Cited by 59 · see citing works
mRNA, a Revolution in Biomedicine
Baptista, B., Carapito, R., Laroui, N., Pichon, C., & de Sousa, F. P. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13122090 · Cited by 59 · see citing works
Advances of mRNA vaccine in tumor: a maze of opportunities and challenges
Yuan, Y., Gao, F., Chang, Y., Zhao, Q., & He, X. (2023) · Biomarker Research · doi:10.1186/s40364-023-00449-w · Cited by 58 · see citing works
A Review on the Stability Challenges of Advanced Biologic Therapeutics
Sarvepalli, S., Pasika, S. R., Verma, V., Thumma, A., Bolla, S., Nukala, P. K., Butreddy, A., & Bolla, P. K. (2025) · Pharmaceutics · doi:10.3390/pharmaceutics17050550 · Cited by 58 · see citing works
- Authors
- Wadhwa, A., Aljabbari, A., Lokras, A., Foged, C., & Thakur, A.
- Affiliations
- University of Copenhagen (DK)
- Corresponding author
- Thakur, Aneesh
- Venue
- Pharmaceutics
- Publisher
- MDPI AG
- Published
- 2020-01-28
- Volume
- 12
- Issue
- 2
- Pages
- 102
- ISSN
- 1999-4923 (ISSN-L)
- PMC
- PMC7076378
- OpenAlex
- W3004416358
- Cited by
- 584
- References
- 184
- Citations by year
- 2026: 55 · 2025: 110 · 2024: 90 · 2023: 109 · 2022: 125 · 2021: 76 · 2020: 19
- FWCI
- 7.87
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Pharmaceutics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Det Frie Forskningsråd (9041-00198B)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- mRNA vaccines, mRNA delivery, mRNA stability, nucleic acid degradation, immune activation, clinical translation, cancer immunotherapy, genetic disease treatment
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
