Advancements in MAFLD Modeling with Human Cell and Organoid Models

Wang, Shi-Xiang; Yan, Ji-Song; Chan, Yun-Shen

doi:10.3390/ijms231911850

Cited by 22Liver Disease Diagnosis and TreatmentLiver physiology and pathologyDrug-Induced Hepatotoxicity and Protection

Advancements in MAFLD Modeling with Human Cell and Organoid Models

Open access

Wang, Shi-Xiang, Yan, Ji-Song, Chan, Yun-Shen·2022·International Journal of Molecular Sciences

DOI 10.3390/ijms231911850PMC PMC9569457

Open this paper in CoChat — free account Open PDF

Abstract

Metabolic (dysfunction) associated fatty liver disease (MAFLD) is one of the most prevalent liver diseases and has no approved therapeutics. The high failure rates witnessed in late-phase MAFLD drug trials reflect the complexity of the disease, and how the disease develops and progresses remains to be fully understood. In vitro, human disease models play a pivotal role in mechanistic studies to unravel novel disease drivers and in drug testing studies to evaluate human-specific responses. This review focuses on MAFLD disease modeling using human cell and organoid models. The spectrum of patient-derived primary cells and immortalized cell lines employed to model various liver parenchymal and non-parenchymal cell types essential for MAFLD development and progression is discussed. Diverse forms of cell culture platforms utilized to recapitulate tissue-level pathophysiology in different stages of the disease are also reviewed.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.3390/ijms231911850

Cites

200 of 200 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Limited translation of preclinical data from animal models due to interspecies differences increases the importance of human-oriented in vitro platforms compatible with controlled design and high-throughput screening.
“Against the background of the limited translation of preclinical data from animal models due to interspecies differences, the importance of human-oriented in vitro platforms compatible with controlled design and high-throughput screening is increasing.”
From The HepG2 Cell Line as a Model for Studying Metabolic Dysfunction-Associated Steatotic Liver Disease (2026) · cites this paper as [27]
Section 1.4 says species-specific differences may hinder translation from animal MAFLD models and calls human in vitro models critical. The Concluding Remarks identify high-throughput drug screening as an application, while Section 4.1 describes precise control of microfluidic models but says their low throughput makes high-throughput screening challenging. The claim therefore overgeneralizes the combination of controlled design and high-throughput compatibility. (LLM, conf=0.85)
Experimental — accuracy not yet measured
The advantages of the MASLD model in HepG2 include reproducibility, preservation of key lipid metabolism pathways, high transfection efficiency, suitability for high-throughput screening, rapid lipid accumulation upon exposure to saturated fatty acids, and low cost.
“The advantages of the MASLD model in this cell line include reproducibility, preservation of key lipid metabolism pathways, high transfection efficiency, suitability for high-throughput screening (HTS), rapid lipid accumulation upon exposure to SFA, low cost, etc. [27,29,79].”
From The HepG2 Cell Line as a Model for Studying Metabolic Dysfunction-Associated Steatotic Liver Disease (2026) · cites this paper as [27]
Section 2.1 (page not specified) describes HepG2 as a stable, widely used MAFLD model and reports lipid accumulation after oleic-acid treatment, including models co-treated with saturated fatty acids. Section 2 also describes stable cell lines as suitable for genetic perturbation. The copy does not establish the claimed high transfection efficiency, preservation of key lipid-metabolism pathways, HepG2-specific high-throughput suitability, rapid lipid accumulation specifically upon saturated-fatty-acid exposure, or low cost. (LLM, conf=0.86)
Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221 · see citing works
Embryonic Stem Cell Lines Derived from Human Blastocysts
Thomson, J. A., Itskovitz‐Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., & Jones, J. M. (1998) · Science · doi:10.1126/science.282.5391.1145 · Cited by 15,947 · see citing works
Design and validation of a histological scoring system for nonalcoholic fatty liver disease†
Kleiner, D. E., Brunt, E. M., Van Natta, M., Behling, C., Contos, M. J., Cummings, O. W., Ferrell, L. D., Liu, Y., Torbenson, M. S., Unalp‐Arida, A., et al. (2005) · Hepatology · doi:10.1002/hep.20701 · Cited by 10,791 · see citing works
Liver fibrosis
Bataller, R., & Brenner, D. A. (2005) · Journal of Clinical Investigation · doi:10.1172/jci24282 · Cited by 4,958 · see citing works
Mitochondria, Oxidants, and Aging
Balaban, R. S., Nemoto, S., & Finkel, T. (2005) · Cell · doi:10.1016/j.cell.2005.02.001 · Cited by 4,388 · see citing works
Steatohepatitis: A tale of two “hits”?
Day, C. P., & James, O. F. W. (1998) · Gastroenterology · doi:10.1016/s0016-5085(98)70599-2 · Cited by 4,285 · see citing works
Nonalcoholic Steatohepatitis: A Proposal for Grading and Staging The Histological Lesions
Brunt, E. M., Janney, C. G., Di Bisceglie, A. M., Neuschwander-Tetri, B. A., & Bacon, B. R. (1999) · American Journal of Gastroenterology · doi:10.1111/j.1572-0241.1999.01377.x · Cited by 3,907 · see citing works
Nonalcoholic fatty liver disease: a spectrum of clinical and pathological severity.
MATTEONI, C., YOUNOSSI, Z., GRAMLICH, T., BOPARAI, N., LIU, Y., & MCCULLOUGH, A. (1999) · Gastroenterology · doi:10.1016/s0016-5085(99)70506-8 · Cited by 3,345 · see citing works
Microfluidic organs-on-chips
Bhatia, S. N., & Ingber, D. E. (2014) · Nature Biotechnology · doi:10.1038/nbt.2989 · Cited by 3,305 · see citing works
MAFLD: a consensus-driven proposed nomenclature for metabolic associated fatty liver disease
Eslam, M., Sanyal, A. J., George, J., Sanyal, A., Neuschwander-Tetri, B., Tiribelli, C., Kleiner, D. E., Brunt, E., Bugianesi, E., Yki-Järvinen, H., et al. (2020) · Gastroenterology · doi:10.1053/j.gastro.2019.11.312 · Cited by 3,286 · see citing works
Modeling the epidemic of nonalcoholic fatty liver disease demonstrates an exponential increase in burden of disease
Estes, C., Razavi, H. A., Loomba, R. S., Younossi, Z. M., & Sanyal, A. J. (2017) · Hepatology · doi:10.1002/hep.29466 · Cited by 2,416 · see citing works
The global epidemiology of NAFLD and NASH in patients with type 2 diabetes: A systematic review and meta-analysis
Younossi, Z. M., Golabi, P., de Avila, L., Paik, J. M., Srishord, M., Fukui, N., Qiu, Y., Burns, L., Afendy, A., & Nader, F. (2019) · Journal of Hepatology · doi:10.1016/j.jhep.2019.06.021 · Cited by 2,235 · see citing works
Vascularized and functional human liver from an iPSC-derived organ bud transplant
Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170 · see citing works
Mechanisms and disease consequences of nonalcoholic fatty liver disease
Loomba, R., Friedman, S. L., & Shulman, G. I. (2021) · Cell · doi:10.1016/j.cell.2021.04.015 · Cited by 2,002 · see citing works
Increased risk of mortality by fibrosis stage in nonalcoholic fatty liver disease: Systematic review and meta‐analysis
Dulai, P. S., Singh, S., Patel, J., Soni, M., Prokop, L. J., Younossi, Z., Sebastiani, G., Ekstedt, M., Hagstrom, H., Nasr, P., et al. (2017) · Hepatology · doi:10.1002/hep.29085 · Cited by 1,965 · see citing works
Resolving the fibrotic niche of human liver cirrhosis at single-cell level
Ramachandran, P., Dobie, R., Wilson-Kanamori, J. R., Dora, E. F., Henderson, B. E. P., Luu, N. T., Portman, J. R., Matchett, K. P., Brice, M., Marwick, J. A., et al. (2019) · Nature · doi:10.1038/s41586-019-1631-3 · Cited by 1,784 · see citing works
Fibrosis Progression in Nonalcoholic Fatty Liver vs Nonalcoholic Steatohepatitis: A Systematic Review and Meta-analysis of Paired-Biopsy Studies
Singh, S., Allen, A. M., Wang, Z., Prokop, L. J., Murad, M. H., & Loomba, R. (2015) · Clinical Gastroenterology and Hepatology · doi:10.1016/j.cgh.2014.04.014 · Cited by 1,748 · see citing works
Progression of NAFLD to diabetes mellitus, cardiovascular disease or cirrhosis
Anstee, Q. M., Targher, G., & Day, C. P. (2013) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2013.41 · Cited by 1,747 · see citing works
Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver
Huch, M., Gehart, H., van Boxtel, R., Hamer, K., Blokzijl, F., Verstegen, M. M. A., Ellis, E., van Wenum, M., Fuchs, S. A., de Ligt, J., et al. (2015) · Cell · doi:10.1016/j.cell.2014.11.050 · Cited by 1,619 · see citing works
Association of nonalcoholic fatty liver disease with insulin resistance
Marchesini, G., Brizi, M., Morselli‐Labate, A. M., Bianchi, G. P., Bugianesi, E., McCullough, A. J., Forlani, G., & Melchionda, N. (1999) · The American Journal of Medicine · doi:10.1016/s0002-9343(99)00271-5 · Cited by 1,564 · see citing works
Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations
MacParland, S. A., Liu, J. C., Ma, X.-Z., Innes, B. T., Bartczak, A. M., Gage, B. K., Manuel, J., Khuu, N., Echeverri, J., Linares, I., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-06318-7 · Cited by 1,552 · see citing works
The liver
Trefts, E., Gannon, M., & Wasserman, D. H. (2017) · Current Biology · doi:10.1016/j.cub.2017.09.019 · Cited by 1,452 · see citing works
Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line
Aden, D. P., Fogel, A., Plotkin, S. A., Damjanov, I., & Knowles, B. B. (1979) · Nature · doi:10.1038/282615a0 · Cited by 1,317 · see citing works
Liver fibrosis and repair: Immune regulation of wound healing in a solid organ
Pellicoro, A., Ramachandran, P., Iredale, J. P., & Fallowfield, J. A. (2014) · Nature Reviews Immunology · doi:10.1038/nri3623 · Cited by 1,310 · see citing works
Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells
Si-Tayeb, K., Noto, F. K., Nagaoka, M., Li, J., Battle, M. A., Duris, C., North, P. E., Dalton, S., & Duncan, S. A. (2010) · Hepatology · doi:10.1002/hep.23354 · Cited by 1,282 · see citing works
Microscale culture of human liver cells for drug development
Khetani, S. R., & Bhatia, S. N. (2008) · Nature Biotechnology · doi:10.1038/nbt1361 · Cited by 1,219 · see citing works
Engineering organoids
Hofer, M., & Lutolf, M. P. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00279-y · Cited by 1,184 · see citing works
Prospective Study of Outcomes in Adults with Nonalcoholic Fatty Liver Disease
Sanyal, A. J., Van Natta, M. L., Clark, J., Neuschwander-Tetri, B. A., Diehl, A., Dasarathy, S., Loomba, R., Chalasani, N., Kowdley, K., Hameed, B., et al. (2021) · New England Journal of Medicine · doi:10.1056/nejmoa2029349 · Cited by 1,146 · see citing works
The role of hepatic lipids in hepatic insulin resistance and type 2 diabetes
Perry, R. J., Samuel, V. T., Petersen, K. F., & Shulman, G. I. (2014) · Nature · doi:10.1038/nature13478 · Cited by 1,136 · see citing works
Gut Microbiome-Based Metagenomic Signature for Non-invasive Detection of Advanced Fibrosis in Human Nonalcoholic Fatty Liver Disease
Loomba, R., Seguritan, V., Li, W., Long, T., Klitgord, N., Bhatt, A., Dulai, P. S., Caussy, C., Bettencourt, R., Highlander, S. K., et al. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.04.001 · Cited by 1,123 · see citing works
Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis
Xu, L., Hui, A. Y., Albanis, E., Arthur, M. J., O’Byrne, S. M., Blaner, W. S., Mukherjee, P., Friedman, S. L., & Eng, F. J. (2004) · Gut · doi:10.1136/gut.2004.042127 · Cited by 1,020 · see citing works
Advances in organ-on-a-chip engineering
Zhang, B., Korolj, A., Lai, B. F. L., & Radisic, M. (2018) · Nature Reviews Materials · doi:10.1038/s41578-018-0034-7 · Cited by 1,017 · see citing works
Genetics and epigenetics of NAFLD and NASH: Clinical impact
Eslam, M., Valenti, L., & Romeo, S. (2018) · Journal of Hepatology · doi:10.1016/j.jhep.2017.09.003 · Cited by 971 · see citing works
The role of macrophages in nonalcoholic fatty liver disease and nonalcoholic steatohepatitis
Kazankov, K., Jørgensen, S. M. D., Thomsen, K. L., Møller, H. J., Vilstrup, H., George, J., Schuppan, D., & Grønbæk, H. (2019) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-018-0082-x · Cited by 941 · see citing works
Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors
Huang, P., He, Z., Ji, S., Sun, H., Xiang, D., Liu, C., Hu, Y., Wang, X., & Hui, L. (2011) · Nature · doi:10.1038/nature10116 · Cited by 864 · see citing works
Fructose consumption as a risk factor for non-alcoholic fatty liver disease
Ouyang, X., Cirillo, P., Sautin, Y. Y., McCall, S. J., Bruchette, J. L., Diehl, A. M., Johnson, R. J., & Abdelmalek, M. F. (2008) · Journal of Hepatology · doi:10.1016/j.jhep.2008.02.011 · Cited by 862 · see citing works
Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors
Sekiya, S., & Suzuki, A. (2011) · Nature · doi:10.1038/nature10263 · Cited by 846 · see citing works
Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids
Hu, H., Gehart, H., Artegiani, B., LÖpez-Iglesias, C., Dekkers, F., Basak, O., van Es, J., Chuva de Sousa Lopes, S. M., Begthel, H., Korving, J., et al. (2018) · Cell · doi:10.1016/j.cell.2018.11.013 · Cited by 837 · see citing works
Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation
Broutier, L., Andersson-Rolf, A., Hindley, C. J., Boj, S. F., Clevers, H., Koo, B.-K., & Huch, M. (2016) · Nature Protocols · doi:10.1038/nprot.2016.097 · Cited by 834 · see citing works
A Protein-Truncating <i>HSD17B13</i> Variant and Protection from Chronic Liver Disease
Abul-Husn, N. S., Cheng, X., Li, A. H., Xin, Y., Schurmann, C., Stevis, P., Liu, Y., Kozlitina, J., Stender, S., Wood, G. C., et al. (2018) · New England Journal of Medicine · doi:10.1056/nejmoa1712191 · Cited by 827 · see citing works
Increased Fructose Consumption Is Associated With Fibrosis Severity in Patients With Nonalcoholic Fatty Liver Disease
Abdelmalek, M. F., Suzuki, A., Guy, C. D., Ünalp–Arida, A., Colvin, R., Johnson, R. J., & Diehl, A. M. (2010) · Hepatology · doi:10.1002/hep.23535 · Cited by 767 · see citing works
Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease
Bell, C. C., Hendriks, D. F. G., Moro, S. M. L., Ellis, E., Walsh, J., Renblom, A., Fredriksson Puigvert, L., Dankers, A. C. A., Jacobs, F., Snoeys, J., et al. (2016) · Scientific Reports · doi:10.1038/srep25187 · Cited by 739 · see citing works
Defective hepatic mitochondrial respiratory chain in patients with nonalcoholic steatohepatitis.
Pérez-Carreras, M., Del Hoyo, P., Martín, M. A., Rubio, J. C., Martín, A., Castellano, G., Colina, F., Arenas, J., & Solis-Herruzo, J. A. (2003) · Hepatology · doi:10.1002/hep.1840380426 · Cited by 734 · see citing works
Molecular phenomics and metagenomics of hepatic steatosis in non-diabetic obese women
Hoyles, L., Fernández-Real, J.-M., Federici, M., Serino, M., Abbott, J., Charpentier, J., Heymes, C., Luque, J. L., Anthony, E., Barton, R. H., et al. (2018) · Nature Medicine · doi:10.1038/s41591-018-0061-3 · Cited by 720 · see citing works
Mouse models in non‐alcoholic fatty liver disease and steatohepatitis research
Anstee, Q. M., & Goldin, R. D. (2006) · International Journal of Experimental Pathology · doi:10.1111/j.0959-9673.2006.00465.x · Cited by 714 · see citing works
Mechanisms of Fibrosis Development in Nonalcoholic Steatohepatitis
Schwabe, R. F., Tabas, I., & Pajvani, U. B. (2020) · Gastroenterology · doi:10.1053/j.gastro.2019.11.311 · Cited by 671 · see citing works
Differential effect of oleic and palmitic acid on lipid accumulation and apoptosis in cultured hepatocytes
Ricchi, M., Odoardi, M. R., Carulli, L., Anzivino, C., Ballestri, S., Pinetti, A., Fantoni, L. I., Marra, F., Bertolotti, M., Banni, S., et al. (2009) · Journal of Gastroenterology and Hepatology · doi:10.1111/j.1440-1746.2008.05733.x · Cited by 652 · see citing works
Directed differentiation of human embryonic stem cells into functional hepatic cells†
Cai, J., Zhao, Y., Liu, Y., Ye, F., Song, Z., Qin, H., Meng, S., Chen, Y., Zhou, R., Song, X., et al. (2007) · Hepatology · doi:10.1002/hep.21582 · Cited by 628 · see citing works
Nonalcoholic Fatty Liver Disease: Pathogenesis and Disease Spectrum
Hardy, T., Oakley, F., Anstee, Q. M., & Day, C. P. (2016) · Annual Review of Pathology Mechanisms of Disease · doi:10.1146/annurev-pathol-012615-044224 · Cited by 619 · see citing works
A Human Hepatocellular in Vitro Model to Investigate Steatosis
Gómez-Lechón, M. J., Donato, M. T., Martínez-Romero, A., Jiménez, N., Castell, J. V., & O’Connor, J.-E. (2007) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2006.11.004 · Cited by 576 · see citing works
Immune cell-mediated features of non-alcoholic steatohepatitis
Huby, T., & Gautier, E. L. (2022) · Nature Reviews Immunology · doi:10.1038/s41577-021-00639-3 · Cited by 573 · see citing works
Animal models of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis
Takahashi, Y. (2012) · World Journal of Gastroenterology · doi:10.3748/wjg.v18.i19.2300 · Cited by 554 · see citing works
Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids
Ouchi, R., Togo, S., Kimura, M., Shinozawa, T., Koido, M., Koike, H., Thompson, W., Karns, R. A., Mayhew, C. N., McGrath, P. S., et al. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.05.007 · Cited by 537 · see citing works
Non-alcoholic fatty liver diseases in patients with COVID-19: A retrospective study
Ji, D., Qin, E., Xu, J., Zhang, D., Cheng, G. Y.-M., Wang, Y., & Lau, G. K. K. (2020) · Journal of Hepatology · doi:10.1016/j.jhep.2020.03.044 · Cited by 524 · see citing works
Emerging therapeutic approaches for the treatment of NAFLD and type 2 diabetes mellitus
Ferguson, D., & Finck, B. N. (2021) · Nature Reviews Endocrinology · doi:10.1038/s41574-021-00507-z · Cited by 507 · see citing works
Cytokines and Nash: A Pilot Study of the Effects of Lifestyle Modification and Vitamin E
Kugelmas, M., Hill, D. B., Vivian, B., Marsano, L. S., & McClain, C. J. (2003) · Hepatology · doi:10.1053/jhep.2003.50316 · Cited by 503 · see citing works
Generation of Functional Hepatocytes From Human Embryonic Stem Cells Under Chemically Defined Conditions That Recapitulate Liver Development
Touboul, T., Hannan, N. R. F., Corbineau, S., Martinez, A., Martinet, C., Branchereau, S., Goulinet, S., Strick‐Marchand, H., Pedersen, R. A., Santo, J. P. D., et al. (2009) · Hepatology · doi:10.1002/hep.23506 · Cited by 499 · see citing works
Inhibition of protein kinase Cε prevents hepatic insulin resistance in nonalcoholic fatty liver disease
Samuel, V. T., Liu, Z.-X., Wang, A., Beddow, S. A., Geisler, J. G., Kahn, M., Zhang, X.-M., Monia, B. P., Bhanot, S., & Shulman, G. I. (2007) · Journal of Clinical Investigation · doi:10.1172/jci30400 · Cited by 497 · see citing works
Animal models of nonalcoholic fatty liver disease
Hebbard, L., & George, J. (2010) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2010.191 · Cited by 495 · see citing works
The portal inflammatory infiltrate and ductular reaction in human nonalcoholic fatty liver disease
Gadd, V. L., Skoien, R., Powell, E. E., Fagan, K. J., Winterford, C., Horsfall, L., Irvine, K., & Clouston, A. D. (2014) · Hepatology · doi:10.1002/hep.26937 · Cited by 452 · see citing works
NAFLD as a driver of chronic kidney disease
Byrne, C. D., & Targher, G. (2020) · Journal of Hepatology · doi:10.1016/j.jhep.2020.01.013 · Cited by 449 · see citing works
Guidelines on the use of liver biopsy in clinical practice from the British Society of Gastroenterology, the Royal College of Radiologists and the Royal College of Pathology
Neuberger, J., Patel, J., Caldwell, H., Davies, S., Hebditch, V., Hollywood, C., Hubscher, S., Karkhanis, S., Lester, W., Roslund, N., et al. (2020) · Gut · doi:10.1136/gutjnl-2020-321299 · Cited by 449 · see citing works
Efficient differentiation of hepatocytes from human embryonic stem cells exhibiting markers recapitulating liver development in vivo
Hay, D. C., Zhao, D., Fletcher, J., Hewitt, Z. A., McLean, D., Urruticoechea-Uriguen, A., Black, J. R., Elcombe, C., Ross, J. A., Wolf, R., et al. (2008) · Stem Cells · doi:10.1634/stemcells.2007-0718 · Cited by 441 · see citing works
Multiple Parallel Hits Hypothesis in Nonalcoholic Fatty Liver Disease: Revisited After a Decade
Tilg, H., Adolph, T. E., & Moschen, A. R. (2020) · Hepatology · doi:10.1002/hep.31518 · Cited by 440 · see citing works
Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation
Mazza, G., Rombouts, K., Rennie Hall, A., Urbani, L., Vinh Luong, T., Al-Akkad, W., Longato, L., Brown, D., Maghsoudlou, P., Dhillon, A. P., et al. (2015) · Scientific Reports · doi:10.1038/srep13079 · Cited by 415 · see citing works
Palmitic acid induces production of proinflammatory cytokine interleukin-8 from hepatocytes
Joshi‐Barve, S., Barve, S. S., Amancherla, K., Gobejishvili, L., Hill, D. B., Cave, M. C., Hote, P., & McClain, C. J. (2007) · Hepatology · doi:10.1002/hep.21752 · Cited by 406 · see citing works
Platelet GPIbα is a mediator and potential interventional target for NASH and subsequent liver cancer
Malehmir, M., Pfister, D., Gallage, S., Szydlowska, M., Inverso, D., Kotsiliti, E., Leone, V., Peiseler, M., Surewaard, B. G. J., Rath, D., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0379-5 · Cited by 398 · cited 1× · see citing works
“In recent years, other immune cells, including neutrophils [134] and platelet cells [135], are also identified to play a role in MAFLD development, prompting a need for such human cell types in in vitro models (further discussed below).”
Background
Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation
Sampaziotis, F., de Brito, M. C., Madrigal, P., Bertero, A., Saeb‐Parsy, K., Soares, F. A. C., Schrumpf, E., Melum, E., Karlsen, T. H., Bradley, J. A., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3275 · Cited by 398 · see citing works
Heritability of Hepatic Fibrosis and Steatosis Based on a Prospective Twin Study
Loomba, R. S., Schork, N. J., Chen, C., Bettencourt, R., Bhatt, A., Ang, B., Nguyen, P., Hernandez, C., Richards, L., Salotti, J., et al. (2015) · Gastroenterology · doi:10.1053/j.gastro.2015.08.011 · Cited by 388 · see citing works
Chemical reprogramming of human somatic cells to pluripotent stem cells.
Guan, J., Wang, G., Wang, J., Zhang, Z., Fu, Y., Cheng, L., Meng, G., Lyu, Y., Zhu, J., Li, Y., et al. (2022) · Nature · doi:10.1038/s41586-022-04593-5 · Cited by 388 · see citing works
Cenicriviroc Treatment for Adults With Nonalcoholic Steatohepatitis and Fibrosis: Final Analysis of the Phase 2b CENTAUR Study
Ratziu, V., Sanyal, A., Harrison, S. A., Wong, V. W., Francque, S., Goodman, Z., Aithal, G. P., Kowdley, K. V., Seyedkazemi, S., Fischer, L., et al. (2020) · Hepatology · doi:10.1002/hep.31108 · Cited by 378 · see citing works
Adaptive immunity: an emerging player in the progression of NAFLD
Sutti, S., & Albano, E. (2020) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-019-0210-2 · Cited by 374 · see citing works
Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids
Mun, S. J., Ryu, J., Lee, M., Son, Y. S., Oh, S. J., Cho, H., Son, M., Kim, S. J., Kim, S. J., Yoo, H. J., et al. (2019) · Journal of Hepatology · doi:10.1016/j.jhep.2019.06.030 · Cited by 369 · see citing works
Role of liver sinusoidal endothelial cells in non-alcoholic fatty liver disease
Hammoutene, A., & Rautou, P.-E. (2019) · Journal of Hepatology · doi:10.1016/j.jhep.2019.02.012 · Cited by 364 · see citing works
Pathological Features of Fatty Liver Disease
Yeh, M. M., & Brunt, E. M. (2014) · Gastroenterology · doi:10.1053/j.gastro.2014.07.056 · Cited by 339 · see citing works
A Gut Microbiome Signature for Cirrhosis Due to Nonalcoholic Fatty Liver Disease
Caussy, C., Tripathi, A., Humphrey, G., Bassirian, S., Singh, S., Faulkner, C., Bettencourt, R., Rizo, E., Richards, L., Xu, Z. Z., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09455-9 · Cited by 338 · see citing works
Presence and significance of microvesicular steatosis in nonalcoholic fatty liver disease
Tandra, S., Yeh, M. M., Brunt, E. M., Vuppalanchi, R., Cummings, O. W., Ünalp–Arida, A., Wilson, L., & Chalasani, N. (2010) · Journal of Hepatology · doi:10.1016/j.jhep.2010.11.021 · Cited by 328 · see citing works
Fructose stimulated de novo lipogenesis is promoted by inflammation
Todoric, J., Di Caro, G., Reibe, S., Henstridge, D. C., Green, C. R., Vrbanac, A., Ceteci, F., Conche, C., McNulty, R., Shalapour, S., et al. (2020) · Nature Metabolism · doi:10.1038/s42255-020-0261-2 · Cited by 328 · see citing works
Directed differentiation of cholangiocytes from human pluripotent stem cells
Ogawa, M., Ogawa, S., Bear, C. E., Ahmadi, S., Chin, S., Li, B., Grompe, M., Keller, G. M., Kamath, B. M., & Ghanekar, A. (2015) · Nature Biotechnology · doi:10.1038/nbt.3294 · Cited by 318 · see citing works
Expression and Characterization of a PNPLA3 Protein Isoform (I148M) Associated with Nonalcoholic Fatty Liver Disease
Huang, Y., Cohen, J. C., & Hobbs, H. H. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.290114 · Cited by 312 · see citing works
Genetic contributions to NAFLD: leveraging shared genetics to uncover systems biology
Eslam, M., & George, J. (2019) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-019-0212-0 · Cited by 310 · see citing works
Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents
Mersch‐Sundermann, V., Knasmüller, S., Wu, X.-J., Darroudi, F., & Kassie, F. (2004) · Toxicology · doi:10.1016/j.tox.2004.02.009 · Cited by 303 · see citing works
Progression from Nonalcoholic Fatty Liver to Nonalcoholic Steatohepatitis Is Marked by a Higher Frequency of Th17 Cells in the Liver and an Increased Th17/Resting Regulatory T Cell Ratio in Peripheral Blood and in the Liver
Rau, M., Schilling, A.-K., Meertens, J., Hering, I., Weiss, J., Jurowich, C., Kudlich, T., Hermanns, H. M., Bantel, H., Beyersdorf, N., et al. (2016) · The Journal of Immunology · doi:10.4049/jimmunol.1501175 · Cited by 302 · see citing works
Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver‐Like Metabolic Biofactories
Bernal, P. N., Bouwmeester, M., Madrid-Wolff, J. A., Falandt, M., Florczak, S., Rodriguez, N. G., Li, Y., Größbacher, G., Samsom, R., van Wolferen, M. E., et al. (2022) · Advanced Materials · doi:10.1002/adma.202110054 · Cited by 290 · see citing works
Accumulation of natural killer T cells in progressive nonalcoholic fatty liver disease
Syn, W.-K., Oo, Y. H., Pereira, T. A., Karaca, G. F., Jung, Y., Omenetti, A., Witek, R. P., Choi, S. S., Guy, C. D., Fearing, C. M., et al. (2010) · Hepatology · doi:10.1002/hep.23599 · Cited by 283 · see citing works
Hepatocyte-Specific Smad7 Expression Attenuates TGF-β–Mediated Fibrogenesis and Protects Against Liver Damage
Dooley, S., Hamzavi, J., Ciuclan, L. I., Godoy, P., Ilkavets, I., Ehnert, S., Ueberham, E., Gebhardt, R., Kanzler, S., Geier, A., et al. (2008) · Gastroenterology · doi:10.1053/j.gastro.2008.04.038 · Cited by 280 · see citing works
Fructose and NAFLD: The Multifaceted Aspects of Fructose Metabolism
Jegatheesan, P., & Bandt, J. P. D. (2017) · Nutrients · doi:10.3390/nu9030230 · Cited by 280 · see citing works
Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease
Ramli, M. N. B., Lim, Y. S., Koe, C. T., Demircioglu, D., Tng, W., Gonzales, K. A. U., Tan, C. P., Szczerbinska, I., Liang, H., Soe, E. L., et al. (2020) · Gastroenterology · doi:10.1053/j.gastro.2020.06.010 · Cited by 278 · see citing works
Self-citation
The global NAFLD policy review and preparedness index: Are countries ready to address this silent public health challenge?
Lazarus, J. V., Mark, H. E., Villota‐Rivas, M., Palayew, A., Carrieri, P., Colombo, M., Ekstedt, M., Esmat, G., George, J., Marchesini, G., et al. (2021) · Journal of Hepatology · doi:10.1016/j.jhep.2021.10.025 · Cited by 270 · see citing works
Mouse liver repopulation with hepatocytes generated from human fibroblasts
Zhu, S.-Y., Rezvani, M., Harbell, J., Mattis, A. N., Wolfe, A., Benet, L., Willenbring, H., & Ding, S. (2014) · Nature · doi:10.1038/nature13020 · Cited by 266 · see citing works
Generation of hepatobiliary organoids from human induced pluripotent stem cells
Wu, F., Wu, D., Ren, Y., Huang, Y., Feng, B., Zhao, N. J., Zhang, T., Chen, X., Chen, S., & Xu, A. (2019) · Journal of Hepatology · doi:10.1016/j.jhep.2018.12.028 · Cited by 264 · see citing works
Pivotal role of liver sinusoidal endothelial cells in NAFLD/NASH progression
Miyao, M., Kotani, H., Ishida, T., Kawai, C., Manabe, S., Abiru, H., & Tamaki, K. (2015) · Laboratory Investigation · doi:10.1038/labinvest.2015.95 · Cited by 255 · see citing works
In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity
Zhang, K., Zhang, L., Liu, W., Ma, X., Cen, J., Sun, Z., Wang, C., Feng, S., Zhang, Z., Yue, L., et al. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.10.018 · Cited by 252 · see citing works
Genetic Factors That Affect Risk of Alcoholic and Nonalcoholic Fatty Liver Disease
Anstee, Q. M., Seth, D., & Day, C. P. (2016) · Gastroenterology · doi:10.1053/j.gastro.2016.01.037 · Cited by 252 · see citing works
Insulin receptor Thr1160 phosphorylation mediates lipid-induced hepatic insulin resistance
Petersen, M. C., Madiraju, A. K., Gassaway, B. M., Marcel, M., Nasiri, A. R., Butrico, G. M., Marcucci, M., Zhang, D., Abulizi, A., Zhang, X., et al. (2016) · Journal of Clinical Investigation · doi:10.1172/jci86013 · Cited by 242 · see citing works
Ballooned hepatocytes in steatohepatitis: The value of keratin immunohistochemistry for diagnosis
Lackner, C., Gogg-Kamerer, M., Zatloukal, K., Stumptner, C., Brunt, E. M., & Denk, H. (2008) · Journal of Hepatology · doi:10.1016/j.jhep.2008.01.026 · Cited by 240 · see citing works
Pnpla3/Adiponutrin deficiency in mice does not contribute to fatty liver disease or metabolic syndrome
Basantani, M. K., Sitnick, M. T., Cai, L., Brenner, D., Gardner, N. P., Li, J. Z., Schoiswohl, G., Yang, K., Kumari, M., Gross, R. W., et al. (2010) · Journal of Lipid Research · doi:10.1194/jlr.m011205 · Cited by 239 · see citing works
Lipid accumulation in hepatocytes induces fibrogenic activation of hepatic stellate cells
Wobser, H., Dorn, C., Weiß, T. S., Amann, T., Bollheimer, C., Büttner, R., Schölmerich, J., & Hellerbrand, C. (2009) · Cell Research · doi:10.1038/cr.2009.73 · Cited by 235 · see citing works
Generation of Hepatic Stellate Cells from Human Pluripotent Stem Cells Enables In Vitro Modeling of Liver Fibrosis
Coll, M., Perea, L., Boon, R., Leite, S. B., Vallverdú, J., Mannaerts, I., Smout, A., El Taghdouini, A., Blaya, D., Rodrigo-Torres, D., et al. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.05.027 · Cited by 233 · see citing works
New insights into the antifibrotic effects of sorafenib on hepatic stellate cells and liver fibrosis
Wang, Y., Gao, J., Zhang, D., Zhang, J., Ma, J., & Jiang, H. (2010) · Journal of Hepatology · doi:10.1016/j.jhep.2010.02.027 · Cited by 232 · see citing works
Comparison of Gene Expression Patterns Between Mouse Models of Nonalcoholic Fatty Liver Disease and Liver Tissues From Patients
Teufel, A., Itzel, T., Erhart, W., Brosch, M., Wang, X. Y., Kim, Y. O., von Schönfels, W., Herrmann, A., Brückner, S., Stickel, F., et al. (2016) · Gastroenterology · doi:10.1053/j.gastro.2016.05.051 · Cited by 229 · see citing works
Intrahepatic Diacylglycerol Content Is Associated With Hepatic Insulin Resistance in Obese Subjects
Magkos, F., Su, X., Bradley, D. P., Fabbrini, E., Conte, C., Eagon, J. C., Varela, J. E., Brunt, E. M., Patterson, B. W., & Klein, S. (2012) · Gastroenterology · doi:10.1053/j.gastro.2012.03.003 · Cited by 223 · see citing works
Association of metabolic dysfunction-associated fatty liver disease with kidney disease
Wang, T.-Y., Wang, R.-F., Bu, Z.-Y., Targher, G., Byrne, C. D., Sun, D.-Q., & Zheng, M.-H. (2022) · Nature Reviews Nephrology · doi:10.1038/s41581-021-00519-y · Cited by 221 · see citing works
A Roadmap for Human Liver Differentiation from Pluripotent Stem Cells
Ang, L. T., Tan, A. K. Y., Autio, M. I., Goh, S. H., Choo, S. H., Lee, K. L., Tan, J., Pan, B., Lee, J. J. H., Lum, J. J., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.01.087 · Cited by 220 · see citing works
Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids
Artegiani, B., van Voorthuijsen, L., Lindeboom, R. G. H., Seinstra, D., Heo, I., Tapia, P., López-Iglesias, C., Postrach, D., Dayton, T., Oka, R., et al. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.04.017 · Cited by 218 · see citing works
The Role of Innate Immune Cells in Nonalcoholic Steatohepatitis
Cai, J., Zhang, X., & Li, H. (2019) · Hepatology · doi:10.1002/hep.30506 · Cited by 207 · see citing works
Risk of severe illness from COVID-19 in patients with metabolic dysfunction-associated fatty liver disease and increased fibrosis scores
Targher, G., Mantovani, A., Byrne, C. D., Wang, X., Yan, H.-D., Sun, Q., Pan, K.-H., Zheng, K. I., Chen, Y.-P., Eslam, M., et al. (2020) · Gut · doi:10.1136/gutjnl-2020-321611 · Cited by 203 · see citing works
A Systematic Review of Animal Models of NAFLD Finds High‐Fat, High‐Fructose Diets Most Closely Resemble Human NAFLD
Im, Y. R., Hunter, H., de Gracia Hahn, D., Duret, A., Cheah, Q., Dong, J., Fairey, M., Hjalmarsson, C., Li, A., Lim, H. K., et al. (2021) · Hepatology · doi:10.1002/hep.31897 · Cited by 195 · see citing works
ROS-Mediated p53 Induction of Lpin1 Regulates Fatty Acid Oxidation in Response to Nutritional Stress
Assaily, W., Rubinger, D. A., Wheaton, K., Lin, Y., Ma, W., Xuan, W., Brown‐Endres, L., Tsuchihara, K., Mak, T. W., & Benchimol, S. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.08.038 · Cited by 192 · see citing works
Current therapies and new developments in NASH
Dufour, J., Anstee, Q. M., Bugianesi, E., Harrison, S. A., Loomba, R. S., Paradis, V., Tilg, H., Wong, V. W., & Zelber‐Sagi, S. (2022) · Gut · doi:10.1136/gutjnl-2021-326874 · Cited by 188 · see citing works
Induced pluripotent stem cell–derived hepatocytes have the functional and proliferative capabilities needed for liver regeneration in mice
Espejel, S., Roll, G. R., McLaughlin, K. J., Lee, A. Y., Zhang, J. Y., Laird, D. J., Okita, K., Yamanaka, S., & Willenbring, H. (2010) · Journal of Clinical Investigation · doi:10.1172/jci43267 · Cited by 187 · see citing works
Quantification and mechanisms of oleic acid-induced steatosis in HepG2 cells.
(2010) · PubMed Central · Cited by 181 · see citing works
Hepatic fat loss in advanced nonalcoholic steatohepatitis: Are alterations in serum adiponectin the cause?
van der Poorten, D., Samer, C. F., Ramezani–Moghadam, M., Coulter, S., Kacevska, M., Schrijnders, D., Wu, L. E., McLeod, D. J., Bugianesi, E., Komuta, M., et al. (2012) · Hepatology · doi:10.1002/hep.26072 · Cited by 178 · see citing works
Investigating Nonalcoholic Fatty Liver Disease in a Liver-on-a-Chip Microfluidic Device
Gori, M., Simonelli, M. C., Giannitelli, S. M., Businaro, L., Trombetta, M., & Rainer, A. (2016) · PLoS ONE · doi:10.1371/journal.pone.0159729 · Cited by 177 · see citing works
Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies
Underhill, G. H., & Khetani, S. R. (2017) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2017.11.012 · Cited by 167 · see citing works
Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens
Fu, G.-B., Huang, W.-J., Zeng, M., Zhou, X., Wu, H.-P., Liu, C.-C., Wu, H., Weng, J., Zhang, H.-D., Cai, Y.-C., et al. (2019) · Cell Research · doi:10.1038/s41422-018-0103-x · Cited by 167 · see citing works
Modelling liver cancer initiation with organoids derived from directly reprogrammed human hepatocytes
Sun, L., Wang, Y., Cen, J., Ma, X., Cui, L., Qiu, Z., Zhang, Z., Li, H., Yang, R., Wang, C., et al. (2019) · Nature Cell Biology · doi:10.1038/s41556-019-0359-5 · Cited by 160 · see citing works
Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
Feaver, R. E., Cole, B. K., Lawson, M. J., Hoang, S. A., Marukian, S., Blackman, B. R., Figler, R. A., Sanyal, A. J., Wamhoff, B. R., & Dash, A. (2016) · JCI Insight · doi:10.1172/jci.insight.90954 · Cited by 153 · see citing works
Human Multilineage 3D Spheroids as a Model of Liver Steatosis and Fibrosis
Pingitore, P., Sasidharan, K., Ekstrand, M., Prill, S., Lindén, D., & Romeo, S. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20071629 · Cited by 152 · see citing works
Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease
Kostrzewski, T. M., Cornforth, T. V., Snow, S., Ouro-Gnao, L., Rowe, C., Large, E. M., & Hughes, D. J. (2017) · World Journal of Gastroenterology · doi:10.3748/wjg.v23.i2.204 · Cited by 149 · see citing works
Endocrine and liver interaction: the role of endocrine pathways in NASH
Loria, P., Carulli, L., Bertolotti, M., & Lonardo, A. (2009) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2009.33 · Cited by 148 · see citing works
Hepatic natural killer T‐cell and CD8+ T‐cell signatures in mice with nonalcoholic steatohepatitis
Bhattacharjee, J., Kirby, M., Softic, S., Miles, L., Salazar‐Gonzalez, R., Shivakumar, P., & Kohli, R. (2017) · Hepatology Communications · doi:10.1002/hep4.1041 · Cited by 146 · see citing works
Diagnosis and Assessment of NAFLD: Definitions and Histopathological Classification
Lackner, C., TINIAKOS, D. G., & Burt, A. D. (2015) · Seminars in Liver Disease · doi:10.1055/s-0035-1562942 · Cited by 145 · see citing works
B2-Lymphocyte Responses to Oxidative Stress-Derived Antigens Contribute to the Evolution of Nonalcoholic Fatty Liver Disease (NAFLD)
Bruzzì, S., Sutti, S., Giudici, G., Burlone, M. E., Ramavath, N. N., Toscani, A., Bozzola, C., Schneider, P., Morello, E., Parola, M., et al. (2018) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2018.06.015 · Cited by 139 · see citing works
Self‐assembled liver organoids recapitulate hepatobiliary organogenesis in vitro
Vyas, D., Baptista, P. M., Brovold, M., Moran, E. C., Gaston, B., Booth, C., Samuel, M., Atala, A., & Söker, S. (2017) · Hepatology · doi:10.1002/hep.29483 · Cited by 134 · see citing works
New insight into inter-organ crosstalk contributing to the pathogenesis of non-alcoholic fatty liver disease (NAFLD)
Zhang, X., Ji, X., Wang, Q., & Li, J. Z. (2017) · Protein & Cell · doi:10.1007/s13238-017-0436-0 · Cited by 131 · see citing works
Precision-cut liver slices: a versatile tool to advance liver research
Palma, E., Doornebal, E. J., & Chokshi, S. (2019) · Hepatology International · doi:10.1007/s12072-018-9913-7 · Cited by 125 · see citing works
The TM6SF2 E167K genetic variant induces lipid biosynthesis and reduces apolipoprotein B secretion in human hepatic 3D spheroids
Prill, S., Caddeo, A. M., Baselli, G., Jamialahmadi, O., Dongiovanni, P., Rametta, R., Kanebratt, K. P., Pujia, A., Pingitore, P., Mancina, R. M., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-47737-w · Cited by 123 · see citing works
CTRP9 transgenic mice are protected from diet-induced obesity and metabolic dysfunction
Peterson, J. M., Wei, Z., Seldin, M. M., Byerly, M. S., Aja, S. M., & Wong, G. W. (2013) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00110.2013 · Cited by 122 · see citing works
Liver macrophages regulate systemic metabolism through non-inflammatory factors
Morgantini, C., Jager, J., Li, X., Levi, L., Azzimato, V., Sulen, A., Barreby, E., Xu, C., Tencerová, M., Näslund, E. B., et al. (2019) · Nature Metabolism · doi:10.1038/s42255-019-0044-9 · Cited by 113 · see citing works
Levels of serum hyaluronic acid, TNF-alpha and IL-8 in patients with nonalcoholic steatohepatitis.
Bahçecioğlu, İ. H., Yalnız, M., Ataseven, H., İlhan, A., Özercan, İ. H., Seçkin, D., & Şahin, K. (2005) · PubMed · Cited by 111 · see citing works
Modeling human nonalcoholic fatty liver disease (NAFLD) with an organoids-on-a-chip system
Wang, Y., Wang, H., Deng, P., Tao, T., Liu, H., Wu, S., Chen, W., & Qin, J. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c00682 · Cited by 109 · see citing works
Modeling Nonalcoholic Fatty Liver Disease with Human Pluripotent Stem Cell-Derived Immature Hepatocyte-Like Cells Reveals Activation of PLIN2 and Confirms Regulatory Functions of Peroxisome Proliferator-Activated Receptor Alpha
Graffmann, N., Ring, S., Kawala, M.-A., Wruck, W., Ncube, A., Trompeter, H., & Adjaye, J. (2016) · Stem Cells and Development · doi:10.1089/scd.2015.0383 · Cited by 109 · see citing works
Three-Dimensional spatially resolved geometrical and functional models of human liver tissue reveal new aspects of NAFLD progression
Segovia-Miranda, F., Morales-Navarrete, H., Kücken, M., Moser, V., Seifert, S., Repnik, U., Rost, F., Brosch, M., Hendricks, A., Hinz, S., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0660-7 · Cited by 109 · see citing works
Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts
Gage, B. K., Liu, J. C., Innes, B. T., MacParland, S. A., McGilvray, I. D., Bader, G. D., & Keller, G. M. (2020) · Cell stem cell · doi:10.1016/j.stem.2020.06.007 · Cited by 104 · see citing works
Human Nonalcoholic Steatohepatitis on a Chip
Freag, M. S., Namgung, B., Reyna Fernandez, M. E., Gherardi, E., Sengupta, S., & Jang, H. L. (2021) · Hepatology Communications · doi:10.1002/hep4.1647 · Cited by 102 · see citing works
Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism
de l’Hortet, A. C., Takeishi, K., Guzman‐Lepe, J., Morita, K., Achreja, A., Popovic, B., Wang, Y., Handa, K., Mittal, A., Meurs, N., et al. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.06.017 · Cited by 101 · see citing works
Gene expression profiling in human precision cut liver slices in response to the FXR agonist obeticholic acid
Ijssennagger, N., Janssen, A. W. F., Milona, A., Pittol, J. M. R., Hollman, D. A. A., Mokrý, M., Betzel, B., Berends, F. J., Janssen, I. M. C., van Mil, S. W. C., et al. (2016) · Journal of Hepatology · doi:10.1016/j.jhep.2016.01.016 · Cited by 93 · see citing works
Maresin 1 attenuates NAFLD by suppression of endoplasmic reticulum stress via AMPK–SERCA2b pathway
Jung, T. W., Kim, H., El‐Aty, A. M. A., & Jeong, J. H. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra117.000885 · Cited by 91 · see citing works
CD4+ T Cells Mediate the Development of Liver Fibrosis in High Fat Diet-Induced NAFLD in Humanized Mice
Her, Z., Tan, J. H. L., Lim, Y.-S., Tan, S. Y., Chan, X., Tan, W., Liu, M., Yong, K. S. M., Lai, F., Ceccarello, E., et al. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.580968 · Cited by 90 · see citing works
Self-citation
Identification of expandable human hepatic progenitors which differentiate into mature hepatic cells in vivo
Nowak, G., Ericzon, B.-G., Nava, S., Jaksch, M., Westgren, M., & Sumitran-Holgersson, S. (2005) · Gut · doi:10.1136/gut.2005.064477 · Cited by 89 · see citing works
Functional Characterization of Organoids Derived From Irreversibly Damaged Liver of Patients With NASH
McCarron, S., Bathon, B., Conlon, D. M., Abbey, D., Rader, D. J., Gawronski, K., Brown, C. D., Olthoff, K. M., Shaked, A., & Raabe, T. D. (2021) · Hepatology · doi:10.1002/hep.31857 · Cited by 89 · see citing works
Modeling alcohol-associated liver disease in a human Liver-Chip
Nawroth, J. C., Petropolis, D. B., Manatakis, D. V., Maulana, T. I., Burchett, G., Schlünder, K., Witt, A., Shukla, A., Kodella, K., Ronxhi, J., et al. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109393 · Cited by 88 · see citing works
Highly Efficient Differentiation of Functional Hepatocytes From Human Induced Pluripotent Stem Cells
Ma, X., Duan, Y., Tschudy-Seney, B., Roll, G. R., Behbahan, I. S., Ahuja, T. P., Tolstikov, V., Wang, C. X., McGee, J., Khoobyari, S., et al. (2013) · Stem Cells Translational Medicine · doi:10.5966/sctm.2012-0160 · Cited by 87 · see citing works
In vitro treatment of HepG2 cells with saturated fatty acids reproduces mitochondrial dysfunction found in non-alcoholic steatohepatitis
García‐Ruíz, I., Solís‐Muñoz, P., Fernández-Moreira, D., Muñoz‐Yagüe, T., & Solis-Herruzo, J. A. (2014) · Disease Models & Mechanisms · doi:10.1242/dmm.018234 · Cited by 83 · see citing works
p38γ and p38δ reprogram liver metabolism by modulating neutrophil infiltration
González‐Terán, B., Matesanz, N., Nikolić, I., Verdugo, M. A., Sreeramkumar, V., Hernández‐Cosido, L., Mora, A., Crainiciuc, G., Sáiz, M. L., Bernardo, E., et al. (2016) · The EMBO Journal · doi:10.15252/embj.201591857 · Cited by 83 · see citing works
Microarray analyses and molecular profiling of steatosis induction in immortalized human hepatocytes
Gottardi, A. D., Vinciguerra, M., Sgroi, A., Moukil, M. A., Ravier, F., Pazienza, V., Pugnale, P., Foti, M., & Hadengue, A. (2007) · Laboratory Investigation · doi:10.1038/labinvest.3700590 · Cited by 79 · see citing works
Modeling PNPLA3‐Associated NAFLD Using Human‐Induced Pluripotent Stem Cells
Tilson, S. G., Morell, C. M., Lenaerts, A., Park, S. B., Hu, Z., Jenkins, B., Koulman, A., Liang, T. J., & Vallier, L. (2021) · Hepatology · doi:10.1002/hep.32063 · Cited by 75 · see citing works
In vitro cellular models of human hepatic fatty acid metabolism: differences between Huh7 and HepG2 cell lines in human and fetal bovine culturing serum
Gunn, P. J., Green, C., Pramfalk, C., & Hodson, L. (2017) · Physiological Reports · doi:10.14814/phy2.13532 · Cited by 74 · see citing works
Enhanced GLUT2 gene expression in an oleic acid-induced in vitro fatty liver model
Okamoto, Y. (2002) · Hepatology Research · doi:10.1016/s1386-6346(01)00172-3 · Cited by 72 · see citing works
C1q/TNF-Related Protein 9 (CTRP9) attenuates hepatic steatosis via the autophagy-mediated inhibition of endoplasmic reticulum stress
Jung, T. W., Hong, H. C., Hwang, H., Yoo, H. J., Baik, S. H., & Choi, K. M. (2015) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2015.09.027 · Cited by 71 · see citing works
Advantageous use of HepaRG cells for the screening and mechanistic study of drug-induced steatosis
Tolosa, L., Gómez‐Lechón, M. J., Jiménez, N., Hervás, D., Jover, R., & Donato, M. T. (2016) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2016.04.007 · Cited by 71 · see citing works
The Role of Leaky Gut in Nonalcoholic Fatty Liver Disease: A Novel Therapeutic Target
Kessoku, T., Kobayashi, T., Tanaka, K., Yamamoto, A., Takahashi, K., Iwaki, M., Ozaki, A., Kasai, Y., Nogami, A., Honda, Y., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22158161 · Cited by 71 · see citing works
Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply
Du, K., Li, S., Li, C., Li, P., Miao, C., Luo, T., Qiu, B., & Ding, W. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.07.013 · Cited by 69 · see citing works
Microengineered cultures containing human hepatic stellate cells and hepatocytes for drug development
Davidson, M. D., Kukla, D. A., & Khetani, S. R. (2017) · Integrative Biology · doi:10.1039/c7ib00027h · Cited by 66 · see citing works
miR-122 inhibition in a human liver organoid model leads to liver inflammation, necrosis, steatofibrosis and dysregulated insulin signaling
Sendi, H., Mead, I., Wan, M., Mehrab‐Mohseni, M., Koch, K. L., Atala, A., Bonkovsky, H. L., & Bishop, C. E. (2018) · PLoS ONE · doi:10.1371/journal.pone.0200847 · Cited by 66 · see citing works
A scalable and sensitive steatosis chip with long-term perfusion of in situ differentiated HepaRG organoids
Teng, Y., Zhao, Z., Tasnim, F., Huang, X., & Yu, H. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.120904 · Cited by 62 · see citing works
Bioengineered Multicellular Liver Microtissues for Modeling Advanced Hepatic Fibrosis Driven Through Non‐Alcoholic Fatty Liver Disease
Cho, H., Kim, H., Lee, K., Lasli, S., Ung, A., Hoffman, T., Nasiri, R., Bandaru, P., Ahadian, S., Dokmeci, M. R., et al. (2021) · Small · doi:10.1002/smll.202007425 · Cited by 62 · see citing works
Mechanism of idiosyncratic drug induced liver injury (DILI): unresolved basic issues
Teschke, R., & Uetrecht, J. (2021) · Annals of Translational Medicine · doi:10.21037/atm-2020-ubih-05 · Cited by 61 · see citing works
LECT2 as a hepatokine links liver steatosis to inflammation via activating tissue macrophages in NASH
Takata, N., Ishii, K., Takayama, H., Nagashimada, M., Kamoshita, K., Tanaka, T., Kikuchi, A., Takeshita, Y., Matsumoto, Y., Ota, T., et al. (2021) · Scientific Reports · doi:10.1038/s41598-020-80689-0 · Cited by 60 · see citing works
A 3D primary human cell-based in vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates
Ströbel, S., Kostadinova, R. M., Fiaschetti‐Egli, K., Rupp, J., Bieri, M., Pawłowska, A., Busler, D., Hofstetter, T., Sanchez, K., Grepper, S., et al. (2021) · Scientific Reports · doi:10.1038/s41598-021-01951-7 · Cited by 58 · see citing works
Three dimensional culture of HepG2 liver cells on a rat decellularized liver matrix for pharmacological studies
Hussein, K. H., Park, K. M., Ghim, J. H., Yang, S. R., & Woo, H. M. (2016) · Journal of Biomedical Materials Research Part B: Applied Biomaterials · doi:10.1002/jbm.b.33384 · Cited by 56 · see citing works
Pathogen- or damage-associated molecular patterns during nonalcoholic fatty liver disease development
Alisi, A., Carsetti, R., & Nobili, V. (2011) · Hepatology · doi:10.1002/hep.24611 · Cited by 56 · see citing works
Poly(vinyl alcohol)/gelatin Hydrogels Cultured with HepG2 Cells as a 3D Model of Hepatocellular Carcinoma: A Morphological Study
Moscato, S., Ronca, F., Campani, D., & Danti, S. (2015) · Journal of Functional Biomaterials · doi:10.3390/jfb6010016 · Cited by 55 · see citing works
In vitro differentiation of pluripotent stem cells into hepatocyte like cells – Basic principles and current progress
Graffmann, N., Scherer, B., & Adjaye, J. (2022) · Stem Cell Research · doi:10.1016/j.scr.2022.102763 · Cited by 54 · see citing works
High-Fat Diet Triggers Mallory-Denk Body Formation Through Misfolding and Crosslinking of Excess Keratin 8
Kücükoglu, Ö., Güldiken, N., Chen, Y., Usachov, V., Heliebi, A. E., Haybaeck, J., Denk, H., Trautwein, C., & Strnad, P. (2014) · Hepatology · doi:10.1002/hep.27068 · Cited by 47 · see citing works
Metabolic Fatty Liver Disease in Children: A Growing Public Health Problem
Garf, S. L., Nègre, V., Anty, R., & Gual, P. (2021) · Biomedicines · doi:10.3390/biomedicines9121915 · Cited by 47 · see citing works
Fatty macroregenerative nodule in non-steatotic liver cirrhosis
Terada, T., Nakanuma, Y., Hoso, M., Saito, K., Sasaki, M., & Nonomura, A. (1989) · Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin · doi:10.1007/bf00784350 · Cited by 47 · see citing works
Specialized Proresolving Mediators: Enhancing Nonalcoholic Steatohepatitis and Fibrosis Resolution
Musso, G., Gambino, R., Cassader, M., Paschetta, E., & Sircana, A. (2018) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2018.01.003 · Cited by 44 · see citing works
Histological assessment based on liver biopsy: the value and challenges in NASH drug development
Tong, X., Wang, Q., Zhao, X., Sun, Y., Wu, X., Yang, L., Lu, Z., Ou, X., Jia, J., & You, H. (2022) · Acta Pharmacologica Sinica · doi:10.1038/s41401-022-00874-x · Cited by 44 · see citing works
Role of immunodeficient animal models in the development of fructose induced NAFLD
Bhattacharjee, J., Kumar, J. M., Arindkar, S., Das, B., Pramod, U., Juyal, R. C., Majumdar, S. S., & Nagarajan, P. (2014) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2013.10.010 · Cited by 44 · see citing works
Exendin-4 alleviates steatosis in an in vitro cell model by lowering FABP1 and FOXA1 expression via the Wnt/-catenin signaling pathway
Khalifa, O., Al-Akl, N. S., Errafii‬, K., & Arredouani, A. (2022) · Scientific Reports · doi:10.1038/s41598-022-06143-5 · Cited by 43 · see citing works
Modelling non-alcoholic fatty liver disease in human hepatocyte-like cells
Lyall, M. J., Cartier, J., Thomson, J. P., Cameron, K., Meseguer-Ripolles, J., O’Duibhir, E., Szkolnicka, D., Lucendo‐Villarin, B., Wang, Y., Blanco, G. R., et al. (2018) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2017.0362 · Cited by 42 · see citing works
WISP1 promotes non‐alcoholic fatty liver disease and skeletal muscle insulin resistance via TLR4/JNK signaling
Jung, T. W., Kang, C. M., Goh, J., Chae, S. I., Kim, H., Lee, T. J., El‐Aty, A. M. A., & Jeong, J. H. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.26449 · Cited by 40 · see citing works
A human pluripotent stem cell model for the analysis of metabolic dysfunction in hepatic steatosis
Sinton, M. C., Meseguer-Ripolles, J., Lucendo‐Villarin, B., Wernig-Zorc, S., Thomson, J. P., Carter, R. N., Lyall, M. J., Walker, P. D., Thakker, A., Meehan, R. R., et al. (2020) · iScience · doi:10.1016/j.isci.2020.101931 · Cited by 40 · see citing works
Humanin attenuates palmitate-induced hepatic lipid accumulation and insulin resistance via AMPK-mediated suppression of the mTOR pathway
Kwon, C., Sun, J. L., Jeong, J. H., & Jung, T. W. (2020) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2020.03.128 · Cited by 40 · see citing works
PU.1 drives specification of pluripotent stem cell-derived endothelial cells to LSEC-like cells
Smedt, J. D., van Os, E. A., Talón, I., Ghosh, S., Toprakhisar, B., da Costa, R. M., Zaunz, S., Vázquez, M. A., Boon, R., Baatsen, P., et al. (2021) · Cell Death and Disease · doi:10.1038/s41419-020-03356-2 · Cited by 39 · see citing works
Sodium valproate blocks the transforming growth factor (TGF)-β1 autocrine loop and attenuates the TGF-β1-induced collagen synthesis in a human hepatic stellate cell line
Watanabe, T. (2011) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2011.768 · Cited by 37 · see citing works
Induced Pluripotent Stem Cell–derived Hepatocytes From Patients With Nonalcoholic Fatty Liver Disease Display a Disease-specific Gene Expression Profile
Duwaerts, C. C., Guillou, D. L., Her, C. L., Phillips, N., Willenbring, H., Mattis, A. N., & Maher, J. J. (2021) · Gastroenterology · doi:10.1053/j.gastro.2021.02.050 · Cited by 36 · see citing works
Emerging liver organoid platforms and technologies
Lam, D. T. U. H., Dan, Y. Y., Chan, Y.-S., & Ng, H. (2021) · Cell Regeneration · doi:10.1186/s13619-021-00089-1 · Cited by 34 · see citing works
Self-citation
Elafibranor restricts lipogenic and inflammatory responses in a human skin stem cell-derived model of NASH
Boeckmans, J., Buyl, K., Natale, A., Vandenbempt, V., Branson, S., Boe, V. D., Rogiers, V., Kock, J. D., Rodrigues, R. M., & Vanhaecke, T. (2019) · Pharmacological Research · doi:10.1016/j.phrs.2019.04.016 · Cited by 32 · see citing works
Bone Morphogenetic Protein-8B Expression is Induced in Steatotic Hepatocytes and Promotes Hepatic Steatosis and Inflammation In Vitro
Mahli, A., Seitz, T., Beckröge, T., Freese, K., Thasler, W. E., Benkert, M., Dietrich, P., Weiskirchen, R., Bosserhoff, A., & Hellerbrand, C. (2019) · Cells · doi:10.3390/cells8050457 · Cited by 30 · see citing works
Stratification of patients in NASH clinical trials: A pitfall for trial success
Ampuero, J., & Romero‐Gómez, M. (2020) · JHEP Reports · doi:10.1016/j.jhepr.2020.100148 · Cited by 30 · see citing works
In Vitro Intestinal and Liver Models for Toxicity Testing
Orbach, S. M., Less, R. R., Kothari, A., & Rajagopalan, P. (2017) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.6b00699 · Cited by 28 · see citing works
A 3D Human Liver Model of Nonalcoholic Steatohepatitis
Duriez, M., Jacquet, A., Hoet, L., Roche, S., Bock, M., Rocher, C., Haussy, G., Vigé, X., Böcskei, Z., Slavnic, T., et al. (2020) · Journal of Clinical and Translational Hepatology · doi:10.14218/jcth.2020.00015 · Cited by 27 · see citing works
Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-β-mediated profibrotic gene expression pattern
Ruehl, M., Erben, U., Kim, K., Freise, C., Dagdelen, T., Eisele, S., Trowitzsch‐Kienast, W., Zeitz, M., Jia, J., Stickel, F., et al. (2008) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2008.06.003 · Cited by 26 · see citing works
An integrated analysis of human myeloid cells identifies gaps in in vitro models of in vivo biology
Rajab, N., Angel, P. W., Deng, Y., Gu, J. L., Jameson, V. J., Kurowska‐Stolarska, M., Milling, S. W. F., Pacheco, C. M., Rutar, M., Laslett, A. L., et al. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.04.010 · Cited by 26 · see citing works
Patatin-like phospholipase domain-containing protein 3 is involved in hepatic fatty acid and triglyceride metabolism through X-box binding protein 1 and modulation of endoplasmic reticulum stress in mice
Ochi, T., Munekage, K., Ono, M., Higuchi, T., Tsuda, M., Hayashi, Y., Okamoto, N., Toda, K., Sakamoto, S., Oben, J. A., et al. (2015) · Hepatology Research · doi:10.1111/hepr.12587 · Cited by 20 · see citing works
Dual role of neutrophils in modulating liver injury and fibrosis during development and resolution of diet-induced murine steatohepatitis
Kim, A. D., Kim, S. E., Leszczynska, A., Kaufmann, B., Reca, A., Kim, D. J., & Feldstein, A. E. (2021) · Scientific Reports · doi:10.1038/s41598-021-03679-w · Cited by 19 · see citing works
Gene Signatures Detect Damaged Liver Sinusoidal Endothelial Cells in Chronic Liver Diseases
Verhulst, S., van Os, E. A., Smet, V. D., Eysackers, N., Mannaerts, I., & van Grunsven, L. A. (2021) · Frontiers in Medicine · doi:10.3389/fmed.2021.750044 · Cited by 18 · see citing works
Development of a cytokine-producing immortalized murine Kupffer cell line
Wang, Z., Burlak, C., Klaunig, J. E., & Kamendulis, L. M. (2014) · Cytokine · doi:10.1016/j.cyto.2014.07.251 · Cited by 17 · see citing works
Changes induced by non-alcoholic fatty liver disease in liver sinusoidal endothelial cells and hepatocytes: spectroscopic imaging of single live cells at the subcellular level
Kochan, K., Kuś, E., Szafraniec, E., Wislocka, A., Chłopicki, S., & Barańśka, M. (2017) · The Analyst · doi:10.1039/c7an00865a · Cited by 16 · see citing works
Arctigenin protects against steatosis in WRL68 hepatocytes through activation of phosphoinositide 3-kinase/protein kinase B and AMP-activated protein kinase pathways
Chen, K.-Y., Lin, J., Yao, H.-Y., Hsu, A.-C., Tai, Y., Chen, J., Hsieh, M.-C., Shen, T., Hsu, R.-Y., Wu, H.-T., et al. (2018) · Nutrition Research · doi:10.1016/j.nutres.2018.02.004 · Cited by 16 · see citing works
Primary hepatocyte cultures for liver disease modeling
Vinken, M. (2020) · Current Opinion in Toxicology · doi:10.1016/j.cotox.2020.08.003 · Cited by 14 · see citing works
Pluripotent Stem Cell-Derived Hepatocytes Phenotypic Screening Reveals Small Molecules Targeting the CDK2/4-C/EBPα/DGAT2 Pathway Preventing ER-Stress Induced Lipid Accumulation
Parafati, M., Bae, S. H., Kirby, R. J., Fitzek, M., Iyer, P., Engkvist, O., Smith, D. M., & Malany, S. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21249557 · Cited by 12 · see citing works
Bioengineered Liver Models for Investigating Disease Pathogenesis and Regenerative Medicine
Kukla, D. A., & Khetani, S. R. (2021) · Seminars in Liver Disease · doi:10.1055/s-0041-1731016 · Cited by 11 · see citing works
Palmitate and Fructose Interact to Induce Human Hepatocytes to Produce Pro-Fibrotic Transcriptional Responses in Hepatic Stellate Cells Exposed to Conditioned Media
Piras, I. S., Gerhard, G. S., & DiStefano, J. K. (2020) · Cellular Physiology and Biochemistry · doi:10.33594/000000288 · Cited by 10 · see citing works
Statins for treatment of chronic liver disease
Marrache, M. K., & Rockey, D. C. (2021) · Current Opinion in Gastroenterology · doi:10.1097/mog.0000000000000716 · Cited by 7 · see citing works
Cellular immortalization and neoplastic transformation
Mondello, C., & Chiodi, I. (2013) · Cell Cycle · doi:10.4161/cc.24940 · Cited by 6 · see citing works
Induced pluripotent stem cell technology: venturing into the second decade
Shi, Y., Inoue, H., Takahashi, J. B., & Yamanaka, S. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818422-6.00095-2 · Cited by 3 · see citing works

Flagged first, then by citations

2 of the 202 references couldn’t be matched to a known paper.

Research works printed but not linked: 1

[152]Burt A.D. Ferrell L.D. Hübscher S.G. MacSween’s Pathology of the Liver-Elsevier 7th ed. Elsevier Amsterdam, The Netherlands 2017

Sorted by citations

Metabolic dysfunction-associated steatotic liver disease-induced changes in the antioxidant system: a review
Svobodová, G., Horní, M., Velecká, E., & Boušová, I. (2024) · Archives of Toxicology · doi:10.1007/s00204-024-03889-x · Cited by 72 · see citing works
Antifibrotic therapy in nonalcoholic steatohepatitis: time for a human-centric approach
Brennan, P. N., Elsharkawy, A. M., Kendall, T. J., Loomba, R., Mann, D. A., & Fallowfield, J. A. (2023) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-023-00796-x · Cited by 68 · see citing works
Human liver cancer organoids: Biological applications, current challenges, and prospects in hepatoma therapy
Chen, L., Wei, X., Gu, D., Xu, Y., & Zhou, H. (2023) · Cancer Letters · doi:10.1016/j.canlet.2022.216048 · Cited by 62 · see citing works
Quercetin alleviates metabolic-associated fatty liver disease by tuning hepatic lipid metabolism, oxidative stress and inflammation
Jiang, L., Yi, R., Chen, H., & Wu, S. (2024) · Animal Biotechnology · doi:10.1080/10495398.2024.2442351 · Cited by 55 · see citing works
Development of LXR inverse agonists to treat MAFLD, NASH, and other metabolic diseases
Griffett, K., & Burris, T. P. (2023) · Frontiers in Medicine · doi:10.3389/fmed.2023.1102469 · Cited by 37 · see citing works
A decade of liver organoids: Advances in disease modeling
Liu, Y., Sheng, J.-Y., Yang, C.-F., Ding, J., & Chan, Y.-S. (2023) · Clinical and Molecular Hepatology · doi:10.3350/cmh.2022.0428 · Cited by 30 · see citing works
Self-citation
Bioactive Compounds from Brown Algae Alleviate Nonalcoholic Fatty Liver Disease: An Extensive Review
Wu, Y., Jin, X., Zhang, Y., Liu, J., Wu, M., & Tong, H. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.2c06578 · Cited by 26 · see citing works
Metabolic dysfunction-associated steatotic liver disease and steatohepatitis-associated hepatocarcinoma preclinical models
Leslie, J., Krishnamurthy, K. A., Gopalsamy, I. K., Inácio, P., Huch, M., Gallage, S., Oakley, F., & Vacca, M. (2026) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-025-01162-9 · Cited by 25 · see citing works
Hepatic organoids as a platform for liver disease modeling and the development of novel therapies
Luce, E., Messina, A., & Duclos‐Vallée, J. (2025) · Clinics and Research in Hepatology and Gastroenterology · doi:10.1016/j.clinre.2025.102647 · Cited by 20 · see citing works
Ex Vivo Tools and Models in MASLD Research
Velliou, R.-I., Giannousi, E., Ralliou, C., Kassi, E., & Chatzigeorgiou, A. (2024) · Cells · doi:10.3390/cells13221827 · Cited by 15 · see citing works
Modeling hepatic steatosis with human adult stem cell-derived liver organoids
Zhu, L., Liu, S., Wang, Z., Yang, Y., Han, P., Tong, W., Zhao, T., Wang, L., Cui, T., Yang, L., et al. (2025) · iScience · doi:10.1016/j.isci.2025.112344 · Cited by 10 · see citing works
Integrating network analysis and pharmacokinetics to investigate the mechanisms of Danzhi Tiaozhi Decoction in metabolic-associated fatty liver disease (MAFLD)
Jiang, X., Tang, N., Liu, Y., Wang, Z., Chen, J., Liu, F., Zhang, P., Sui, M., & Xu, W. G. (2023) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2023.117008 · Cited by 10 · see citing works
MASLD, diabetes and PMOS across the female life stages
Stener‐Victorin, E., Carlsson, S., Hagström, H., & Taebnia, N. (2026) · Diabetologia · doi:10.1007/s00125-026-06761-y · Cited by 6 · see citing works
The HepG2 Cell Line as a Model for Studying Metabolic Dysfunction-Associated Steatotic Liver Disease
Kotlyarova, A., Iskrina, A., & Kotlyarov, S. (2026) · International journal of molecular sciences · doi:10.3390/ijms27083399 · Cited by 5 · see citing works
Honokiol Mitigates Metabolic-Associated Fatty Liver Disease by Regulating Nrf2 and RIPK3 Signaling Pathways
Cao, W., Chen, Z., Lin, C., Lin, X., Chen, Y., & Zhang, J. (2024) · The Turkish Journal of Gastroenterology · doi:10.5152/tjg.2024.23470 · Cited by 4 · see citing works
Engineering a three-dimensional liver steatosis model
Johnston, E. K., Zhou, F., Soto–Gutiérrez, A., Taner, C. B., Cook, K. E., Liu, Y., & Abbott, R. D. (2025) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2025.167888 · Cited by 3 · see citing works
Hepatocyte-like cells and liver organoids: the application of iPSCs and their derivants for treating liver diseases
Ju, R., Tian, S., Shang, Y., Ma, S., Zhang, M., Liu, J., Sun, K., Cui, L., Zhou, X., & Han, Y. (2024) · Materials Advances · doi:10.1039/d4ma00373j · Cited by 2 · see citing works
Advanced models to study liver sinusoids
Campinoti, S., Palma, E., Chokshi, S., & Urbani, L. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-95262-0.00007-3 · Cited by 1 · see citing works
Community Series - Liver Fibrosis and MAFLD: from Molecular Aspects to Novel Pharmacological Strategies, Volume II
(2024) · Frontiers research topics · doi:10.3389/978-2-8325-4473-0
Modeling Steatohepatitis with Human Adult Stem Cell-Derived Liver OrganoidsPreprint
Zhu, L., Liu, S., Tong, W., Yang, Y., Han, P., Wang, Z., Zhao, T., Wang, L., Cui, T., Yang, L., et al. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4848267
Assessment of Knowledge, Attitudes and Practices on Metabolic Dysfunction Associated Fatty Liver Disease (MAFLD) Among Community: A Hospital-Based Analytical Cross-Sectional StudyPreprint
Bolla, H., Aradya, H. V., & Shilpa, D. M. (2026) · Research Square · doi:10.21203/rs.3.rs-10098823/v1
Suppressor with morphogenetic effect on genitalia 7 (SMG7) drives hepatocellular carcinoma progression through context-dependent nonsense-mediated decay (NMD) dysregulation
Ojeda-Perez, B., Fernandez-Ramirez, V. J., Pozo-Relano, M. I., Gracia-Herencia, B., Hawro, I., Córdoba-Chacón, J., Luque, R. M., Rodriguez-Peralvarez, M. L., Lopez-Canovas, J. L., & Gahete, M. D. (2026) · Molecular Biomedicine · doi:10.1186/s43556-026-00577-3

Primary Human Tissue Models for Metabolic Dysfunction‐Associated Liver Disease ‐ toward Streamlining Drug Discovery with Patient‐Derived Assays
Youhanna, S., Taebnia, N., Liang, Y., Cheng, N., Wang, Y., Michel, M., & Lauschke, V. M. (2025) · Advanced Biology · doi:10.1002/adbi.202500337 · Cited by 9 · see citing works
From fatty hepatocytes to impaired bile flow: Matching model systems for liver biology and disease
Kunst, R. F., Niemeijer, M., van der Laan, L. J. W., Spee, B., & van de Graaf, S. F. J. (2020) · Biochemical Pharmacology · doi:10.1016/j.bcp.2020.114173 · Cited by 9 · see citing works
Research progress on animal models of metabolic dysfunction-associated fatty liver disease
Lin-Yun, Z. H. A. O., Zhang, X., Zi-Meng, X. I., ZHANG, Q., Chun-Li, M. A., & Bao, Y. (2026) · Shengming kexue · doi:10.3724/cbls.2026017
Metabolic dysfunction-associated fatty liver disease (MAFLD) : Development of a relevant in vitro model
Merheb, C. (2024) · doi:10.70675/586dfe12z71ffz499bza426z93faada5a8c6
Organoids as preclinical platforms for metabolic dysfunction-associated steatotic liver disease: Model construction, mechanistic dissection, and therapeutic translation
Xue, H.-Y., Ren, T., Pan, Q., & Fan, J.-G. (2026) · World Journal of Gastroenterology · doi:10.3748/wjg.123934

Authors: Wang, S.-X., Yan, J.-S., & Chan, Y.-S.
Affiliations: Guangzhou Laboratory, No. 9 Xing Dao Huan Bei Road, Guangzhou International Bio Island, Guangzhou 510005, China
Yunnan University (CN)
Guangzhou Laboratory (CN)
Corresponding author: Chan, Yun-Shen
ORCID: Chan, Yun-Shen
Venue: International Journal of Molecular Sciences
Publisher: MDPI AG
Published: 2022-10-06
Volume: 23
Issue: 19
Pages: 11850
ISSN: 1422-0067 (ISSN-L)
DOI: 10.3390/ijms231911850
PMC: PMC9569457
OpenAlex: W4303699718
Cited by: 22
References: 202
Citations by year: 2026: 5 · 2025: 3 · 2024: 8 · 2023: 6
FWCI: 0.68
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
Locations: International Journal of Molecular Sciences (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics: Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
Keywords: non-alcoholic fatty liver disease, disease modeling, human organoids, patient-derived primary cells, immortalized cell lines, liver parenchymal cells, non-parenchymal liver cells, in vitro disease model, drug testing, disease progression
MeSH terms: Humans, Liver Diseases, Organoids, Cell Culture Techniques, Non-alcoholic Fatty Liver Disease

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Advancements in MAFLD Modeling with Human Cell and Organoid Models

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Self-check

Cites

Cited by

Related

Record

About these checks