Hesperidin contributes to the vascular protective effects of orange juice: a randomized crossover study in healthy volunteers
Open accessAbstract
Background Although numerous human studies have shown consistent effects of some polyphenol-rich foods on several intermediate markers for cardiovascular diseases, it is still unknown whether their action could be specifically related to polyphenols.Objective We investigated the effect of orange juice and its major flavonoid, hesperidin, on microvascular reactivity, blood pressure, and cardiovascular risk biomarkers through both postprandial and chronic intervention studies.Design Twenty-four healthy, overweight men (age 50-65 y) were included in a randomized, controlled, crossover study. Throughout the three 4-wk periods, volunteers daily consumed 500 mL orange juice, 500 mL control drink plus hesperidin (CDH), or 500 mL control drink plus placebo (CDP). All measurements and blood collections were performed in overnight-fasted subjects before and after the 4-wk treatment periods. The postprandial study was conducted at the beginning of each experimental period.Results Diastolic blood pressure (DBP) was significantly lower after 4 wk consumption of orange juice or CDH than after consumption of CDP (P = 0.02), whereas microvascular endothelium-related reactivity was not significantly affected when measured after an overnight fast. However, both orange juice and CDH ingestion significantly improved postprandial microvascular endothelial reactivity compared with CDP (P < 0.05) when measured at the peak of plasma hesperetin concentration.Conclusions In healthy, middle-aged, moderately overweight men, orange juice decreases DBP when regularly consumed and postprandially increases endothelium-dependent microvascular reactivity. Our study suggests that hesperidin could be causally linked to the beneficial effect of orange juice. This trial is registered at clinicaltrials.gov as NCT00983086.
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Intervention studies comparing a whole food with one of its bioactive constituents have demonstrated that these compounds may contribute to the health effects of the food.
“Although these compounds may actually contribute to the health effects of the food, as has been well demonstrated in intervention studies in which a whole food has been compared with one of its bioactive constituents (177)”
From The food metabolome: a window over dietary exposure (2014) · cites this paper as [177]
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- Authors
- Morand, C., Dubray, C., Milenkovic, D., Lioger, D., Martin, J. F., Scalbert, A., & Mazur, A.
- Affiliations
- Université Clermont Auvergne (FR)
- Inserm (FR)
- Unité de Nutrition Humaine (FR)
- Venue
- The American Journal of Clinical Nutrition
- Publisher
- Elsevier BV
- Published
- 2011-01
- Volume
- 93
- Issue
- 1
- Pages
- 73-80
- ISSN
- 0002-9165 (ISSN-L)
- OpenAlex
- W2144628587
- Cited by
- 439
- References
- 50
- Citations by year
- 2026: 14 · 2025: 16 · 2024: 15 · 2023: 31 · 2022: 25 · 2021: 42 · 2020: 32 · 2019: 47 · 2018: 21 · 2017: 32 · 2016: 28 · 2015: 32 · 2014: 31 · 2013: 31 · 2012: 25
- FWCI
- 14.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- American Journal of Clinical Nutrition (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Bioactive Compounds in Plants
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
- Keywords
- orange juice, hesperidin, hesperetin, flavonoids, polyphenols, diastolic blood pressure, cardiovascular risk markers, postprandial effects, overweight men, dietary intervention
- MeSH terms
- Aged, Antioxidants, Beverages, C-Reactive Protein, Diastole, Endothelium, Vascular, Hesperidin, Humans, Male, Middle Aged, Vasodilation, Oxidative Stress, Cross-Over Studies, Citrus sinensis
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