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Rab10 regulates the sorting of internalised TrkB for retrograde axonal transport

Lazo, Oscar Marcelo, Schiavo, Giampietro·2023·eLife
DOI 10.7554/elife.81532PMC PMC10005780

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Abstract

Neurons process real-time information from axon terminals to coordinate gene expression, growth, and plasticity. Inputs from distal axons are encoded as a stream of endocytic organelles, termed signalling endosomes, targeted to the soma. Formation of these organelles depends on target-derived molecules, such as brain-derived neurotrophic factor (BDNF), which is recognised by TrkB receptors on the plasma membrane, endocytosed, and transported to the cell body along the microtubules network. Notwithstanding its physiological and neuropathological importance, the mechanism controlling the sorting of TrkB to signalling endosomes is currently unknown. In this work, we use primary mouse neurons to uncover the small GTPase Rab10 as critical for TrkB sorting and propagation of BDNF signalling from axon terminals to the soma. Our data demonstrate that Rab10 defines a novel membrane compartment that is rapidly mobilised towards the axon terminal upon BDNF stimulation, enabling the axon to fine-tune retrograde signalling depending on BDNF availability at the synapse. These results help clarifying the neuroprotective phenotype recently associated to Rab10 polymorphisms in Alzheimer’s disease and provide a new therapeutic target to halt neurodegeneration.

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Crossref · 10.7554/elife.81532

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  • LRRK2 activity plays a role in synaptic vesicle endocytosis, activation of autophagy, and axonal transport of growth factor receptors.

    “LRRK2 activity plays a role in synaptic vesicle endocytosis, activation of autophagy, and axonal transport of growth factor receptors [2, 7, 10, 24, 25, 36, 51, 59, 62, 75, 77, 96].”

    From Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brain (2023) · cites this paper as [36]

    The Discussion links LRRK2 activity to Rab10 phosphorylation and discusses possible effects on the axonal trafficking of TrkB, a growth factor receptor, but says the relevant transport mechanism remains under investigation. A reference title mentions LRRK2 in synaptic endocytosis; the paper's discussion does not establish the synaptic-vesicle claim, and the supplied passages do not support the autophagy claim. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

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Metadata

Authors
Lazo, O. M., & Schiavo, G.
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2023-03-10
Volume
12
ISSN
2050-084X
DOI
10.7554/elife.81532
PMC
PMC10005780
Cited by
26
References
2
Language
en
Licence
Funders
  • Medical Research Council (MR/T001976/1)
  • Wellcome Trust (107116/Z/15/Z, 223022/Z/21/Z)
  • UK Dementia Research Institute Foundation (UKDRI-1005)

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