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Cited by 219Proteoglycans and glycosaminoglycans researchNeurogenesis and neuroplasticity mechanismsOsteoarthritis Treatment and Mechanisms

The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.

Differential regulation of expression of hyaluronan-binding proteoglycans in developing brain: aggrecan, versican, neurocan, and brevican.

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Milev, Peter, Maurel, Patrice, Chiba, Atsuro, Mevissen, Markus, Popp, Susanna, Yamaguchi, Yu, Margolis, Renée K., Margolis, Richard U.·1998·Biochemical and Biophysical Research Communications
DOI 10.1006/bbrc.1998.8759
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Abstract

We have used a slot-blot radioimmunoassay to quantitate the levels of hyaluronan-binding chondroitin sulfate proteoglycans in developing rat brain from embryonic day 14 (E 14) to eight months postnatal. Recombinant nonhomologous regions of the core proteins were used for immunization to obtain polyclonal antibodies specific for aggrecan, the alpha and beta domains of versican mRNA splice variants, and N- and C-terminal portions of neurocan, while brevican was quantitated using a specific monoclonal antibody. The concentration of aggrecan increased steadily during brain development up to 5 months of age, when it reached a level that was 18-fold higher than at E14. Alternatively spliced versican isoforms containing the alpha domain of the glycosaminoglycan attachment region were present at a relatively low level during the late embryonic and early postnatal period, decreased by approximately 50% between 1 and 2 weeks postnatal, and then increased steadily in concentration to reach a maximum at 100 days that was 7-fold that present at 10 days postnatal. In contrast to these results, versican isoforms containing the beta domain more than doubled in concentration between E14 and birth, after which they decreased by greater than 90% to reach a low "mature" level that remained unchanged between 2 and 8 months. The N- and C-terminal portions of neurocan (produced by a developmentally-regulated proteolytic cleavage in the middle of its chondroitin sulfate attachment region) both increased in embryonic brain during development, reached a peak in the early postnatal period, and then declined thereafter. As in the case of aggrecan, only traces of brevican were detected in embryonic brain and its concentration increased steadily after birth to reach an adult level that was approximately 14-fold higher than that present in neonatal brain. These striking and distinctive changes in the concentrations of the different members of this family of structurally related proteoglycans in developing brain, including changes in opposite directions for versican mRNA splice variants, indicate that the individual proteoglycans and their isoforms probably serve unique functions during nervous tissue histogenesis.

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Research works printed but not linked: 4

  1. Schwartz, 1996, Perspect. Dev. Neurobiol., 3, 291
  2. LeBaron, 1996, Perspect. Dev. Neurobiol., 3, 261
  3. Margolis, 1996, Perspect. Dev. Neurobiol., 3, 273
  4. Yamaguchi, 1996, Perspect. Dev. Neurobiol., 3, 307

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Metadata

Authors
Milev, P., Maurel, P., Chiba, A., Mevissen, M., Popp, S., Yamaguchi, Y., Margolis, R. K., & Margolis, R. U.
Affiliations
  • NYU Langone Health (US)
  • State University of New York (US)
  • Sanford Burnham Prebys Medical Discovery Institute (US)
Corresponding author
Margolis, Richard U.
ORCID
Yamaguchi, Yu
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
1998-06
Volume
247
Issue
2
Pages
207-212
ISSN
0006-291X (ISSN-L)
DOI
10.1006/bbrc.1998.8759
OpenAlex
W2137502661
Cited by
219
References
20
Citations by year
2026: 4 · 2025: 4 · 2024: 7 · 2023: 6 · 2022: 3 · 2021: 6 · 2020: 11 · 2019: 8 · 2018: 9 · 2017: 6 · 2016: 6 · 2015: 8 · 2014: 10 · 2013: 8 · 2012: 10
FWCI
3.74
Type
journal article
Language
en
Open access
Licence
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
Keywords
chondroitin sulfate proteoglycans, brain development, aggrecan, versican, neurocan, brevican, splice variants, proteolytic cleavage, embryonic brain, postnatal brain
MeSH terms
Animals, Brain, Carrier Proteins, Hyaluronic Acid, Nerve Tissue Proteins, Chondroitin Sulfate Proteoglycans, Proteoglycans, RNA, Messenger, Extracellular Matrix Proteins, Alternative Splicing, Gene Expression Regulation, Developmental, Lectins, C-Type, Rats, Aggrecans, Versicans, Neurocan, Brevican

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