The nicotinic antagonist mecamylamine precipitates nicotine abstinence syndrome in the rat
Abstract
Recently, a rodent model of nicotine abstinence syndrome has been developed based on observing the frequency of spontaneous behavioral signs following termination of continuous subcutaneous infusion of nicotine tartrate. In the present study, the nicotinic antagonist mecamylamine precipitated an abstinence syndrome in nicotine-dependent rats. Twelve rats were each infused for 7 days with 9 mg/kg per day nicotine tartrate in saline via Alzet osmotic minipumps; another 12 rats were sham-operated and remained nicotine-naive. Six rats from each group received 1 mg/kg mecamylamine in saline SC immediately before a 30-min observation, while the remaining six rats from each group received saline alone. Nicotine-infused rats receiving mecamylamine exhibited significantly more (P < 0.01), overall abstinence signs than all other groups. In terms of categories of signs, they displayed significantly more gasps/writhes, teeth chatter/chews, shakes/tremors and ptosis. In a second experiment utilizing only nicotine-naive rats, a far higher dose of mecamylamine (5 mg/kg sc) induced a quasi-nicotine abstinence syndrome. The results provide further validation for this rodent model of nicotine abstinence syndrome.
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In mice, after at least a week of chronic nicotine, stopping nicotine or giving an nAChR antagonist produces affective, somatic, and cognitive withdrawal signs.
“After at least a week of chronic nicotine administration in mice, cessation of nicotine exposure or administration of a nAChR antagonist induces affective, somatic, and cognitive signs of withdrawal (Malin et al. 1994; De Biasi and Salas 2008).”
From Nicotine Withdrawal (2015) · cites this paper as Malin et al. 1994
Cites
Smoking withdrawal symptoms in two weeks of abstinence
Shiffman, S. M., & Jarvik, M. E. (1976) · Psychopharmacology · doi:10.1007/bf00634151 · Cited by 548 · see citing works
The effects of nicotine on locomotor activity in non‐tolerant and tolerant rats
Clarke, P. B. S., & Kumar, R. (1983) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1983.tb09398.x · Cited by 457 · see citing works
Symptoms of Tobacco Withdrawal: A Replication and Extension
Hughes, J. R. (1991) · Archives of General Psychiatry · doi:10.1001/archpsyc.1991.01810250054007 · Cited by 449 · see citing works
Rodent model of nicotine abstinence syndrome
Malin, D. H., Lake, J. R., Newlin-Maultsby, P., Roberts, L. K., Lanier, J. G., Carter, V. A., Cunningham, J. S., & Wilson, O. B. (1992) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(92)90408-8 · Cited by 334 · see citing works
Self-citationMarks, M. J., & Collins, A. C. (1982) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)15183-3 · Cited by 310 · see citing works
QUANTITATIVE ASPECTS OF PRECIPITATED ABSTINENCE IN MORPHINE-DEPENDENT RATS
Wei, E. T., Loh, H. H., & Way, E. L. (1973) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)29423-x · Cited by 257 · see citing works
Nicotine-induced antinociception in rats and mice: correlation with nicotine brain levels.
Tripathi, H. L., Martin, B. R., & Aceto, M. D. G. (1982) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)33022-3 · Cited by 171 · see citing works
Neuroendocrine reactivity to nicotine in smokers
Pomerleau, O. F., Fertig, J. B., SEYLER, L. E., & Jaffe, J. H. (1983) · Psychopharmacology · doi:10.1007/bf00439275 · Cited by 155 · see citing works
Naloxone precipitates nicotine abstinence syndrome in the rat
Malin, D. H., Lake, J. R., Carter, V. A., Cunningham, J. S., & Wilson, O. B. (1993) · Psychopharmacology · doi:10.1007/bf02244930 · Cited by 147 · see citing works
Self-citationDavenport, K. E., Houdi, A. A., & Loon, G. R. V. (1990) · Neuroscience Letters · doi:10.1016/0304-3940(90)90491-q · Cited by 145 · see citing works
Nicotine and the central nervous system: Biobehavioral effects of cigarette smoking
Pomerleau, O. F. (1992) · The American Journal of Medicine · doi:10.1016/0002-9343(92)90619-m · Cited by 139 · see citing works
Effects of the nicotinic receptor blocker mecamylamine on radial-arm maze performance in rats
Levin, E. D., Castonguay, M., & Ellison, G. D. (1987) · Behavioral and Neural Biology · doi:10.1016/s0163-1047(87)90752-7 · Cited by 112 · see citing works
Chronic nicotine and withdrawal effects on body weight and food and water consumption in female rats
Levin, E. D., Morgan, M. M., Galvez, C., & Ellison, G. D. (1987) · Physiology & Behavior · doi:10.1016/0031-9384(87)90370-2 · Cited by 102 · see citing works
What is the nature of mecamylamine's antagonism of the central effects of nicotine?
Martin, B. R., Onaivi, E. S., & Martin, T. J. (1989) · Biochemical Pharmacology · doi:10.1016/0006-2952(89)90106-8 · Cited by 92 · see citing works
EEG Changes During Tobacco Withdrawal
Herning, R. I., Jones, R. T., & Bachman, J. (1983) · Psychophysiology · doi:10.1111/j.1469-8986.1983.tb03004.x · Cited by 89 · see citing works
Nicotine cue in rats analysed with drugs acting on cholinergic and 5-hydroxytryptamine mechanisms
Stolerman, I. P., Pratt, J. A., Garcha, H. S., et al. (1983) · Neuropharmacology · doi:10.1016/0028-3908(83)90021-7 · Cited by 85 · see citing works
Helton, D. R., Modlin, D. L., Tizzano, J. P., & Rasmussen, K. (1993) · Psychopharmacology · doi:10.1007/bf02245698 · Cited by 81 · see citing works
Neuroregulatory effects of nicotine
Pomerleau, O. F., & Rosecrans, J. A. (1989) · Psychoneuroendocrinology · doi:10.1016/0306-4530(89)90040-1 · Cited by 79 · see citing works
Mecamylamine increases nicotine preference and attenuates nicotine discrimination
Rose, J. E., Sampson, A. P., Levin, E. D., & Henningfield, J. E. (1989) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(89)90061-0 · Cited by 79 · see citing works
Pierzchała, K., Houdi, A. A., & Loon, G. R. V. (1987) · Peptides · doi:10.1016/0196-9781(87)90133-1 · Cited by 76 · see citing works
[3H]mecamylamine binding to rat brain membranes
Banerjee, S., Punzi, J. S., Kreilick, K. G., et al. (1990) · Biochemical Pharmacology · doi:10.1016/0006-2952(90)90241-c · Cited by 55 · see citing works
EFFECTS OF NICOTINE ON FIXED-INTERVAL BEHAVIOR AND THEIR MODIFICATION BY CHOLINERGIC ANTAGONISTS
Stitzer, M. L., MORRISON, J., & Domino, E. F. (1970) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)28461-0 · Cited by 49 · see citing works
Martin, T. J., Suchocki, J. A., May, E. L., & Martin, B. R. (1990) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)12619-0 · Cited by 47 · see citing works
Evidence for a behavioral deficit during withdrawal from chronic nicotine treatment
Corrigall, W. A., Herling, S., & Coen, K. M. (1989) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(89)90387-0 · Cited by 44 · see citing works
Rosecrans, J. A., Hendry, J. S., & Hong, J. (1985) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(85)90141-8 · Cited by 42 · see citing works
Auricular microelectrostimulation: Naloxone-reversible attenuation of opiate abstinence syndrome
Malin, D. H., Murray, J. B., Crucian, G. P., Schweitzer, F. C., Cook, R. E., & Skolnick, M. H. (1988) · Biological Psychiatry · doi:10.1016/0006-3223(88)90223-5 · Cited by 41 · see citing works
Self-citationHall, G. (1972) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1972.tb07303.x · Cited by 39 · see citing works
Carroll, M. E., Lac, S. T., Asencio, M., & Keenan, R. M. (1989) · Life Sciences · doi:10.1016/0024-3205(89)90025-8 · Cited by 37 · see citing works
Jensen, R. A., Gilbert, D. G., Meliska, C. J., Landrum, T. A., & Szary, A. (1990) · Behavioral and Neural Biology · doi:10.1016/0163-1047(90)90310-3 · Cited by 34 · see citing works
Meltzer, L. T., & Rosecrans, J. A. (1981) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(81)90332-4 · Cited by 33 · see citing works
Plasma enkephalin-like peptide response to chronic nicotine infusion in guinea pig
Hexum, T. D., & Russett, L. R. (1987) · Brain Research · doi:10.1016/0006-8993(87)90809-2 · Cited by 27 · see citing works
Wu, K. M., & Martin, W. R. (1983) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)22006-7 · Cited by 25 · see citing works
Nicotine-induced tail-tremor and drug effects
Gomita, Y., Suemaru, K., Furuno, K., & Araki, Y. (1989) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(89)90280-3 · Cited by 22 · see citing works
NICOTINE INDUCED TREMOR AND ANTIDIURESIS AND BRAIN NICOTINE LEVELS IN RATS
Mansner, R. (1977) · Abstracts · doi:10.1016/b978-0-08-021308-8.51271-0 · Cited by 16 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Guidelines on nicotine dose selection for in vivo research
Matta, S. G., Balfour, D. J., Benowitz, N. L., Boyd, R. T., Buccafusco, J. J., Caggiula, A. R., Craig, C. R., Collins, A. C., Damaj, M. I., Donny, E. C., et al. (2007) · Psychopharmacology · doi:10.1007/s00213-006-0441-0 · Cited by 824 · see citing works
Neurobiological similarities in depression and drug dependence: a self-medication hypothesis
Markou, A. (1998) · Neuropsychopharmacology · doi:10.1016/s0893-133x(97)00113-9 · Cited by 767 · see citing works
Dramatic decreases in brain reward function during nicotine withdrawal
Epping-Jordan, M. P., Watkins, S. S., Koob, G. F., & Markou, A. (1998) · Nature · doi:10.1038/30001 · Cited by 674 · see citing works
Neural mechanisms underlying nicotine addiction: acute positive reinforcement and withdrawal
S. Watkins, G. F. K. (2000) · Nicotine & Tobacco Research · doi:10.1080/14622200050011277 · Cited by 424 · see citing works
Salas, R., Sturm, R., Boulter, J., & De Biasi, M. (2009) · The Journal of Neuroscience · doi:10.1523/jneurosci.4934-08.2009 · Cited by 310 · see citing works
Neurobiology of the nicotine withdrawal syndrome
Kenny, P. J., & Markou, A. (2001) · Pharmacology Biochemistry and Behavior · doi:10.1016/s0091-3057(01)00651-7 · Cited by 310 · see citing works
The putative role of extra-synaptic mesolimbic dopamine in the neurobiology of nicotine dependence
Balfour, D. J. K., Wright, A. E., Benwell, M. E. M., & Birrell, C. E. (2000) · Behavioural Brain Research · doi:10.1016/s0166-4328(00)00202-3 · Cited by 231 · see citing works
Salas, R., Pieri, F., & De Biasi, M. (2004) · The Journal of Neuroscience · doi:10.1523/jneurosci.1939-04.2004 · Cited by 226 · see citing works
Watkins, S. S., Stinus, L., Koob, G. F., & Markou, A. (2000) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)35389-3 · Cited by 214 · see citing works
Hildebrand, B. E., Nomikos, G. G., Hertel, P., Schilström, B., & Svensson, T. H. (1998) · Brain Research · doi:10.1016/s0006-8993(97)01135-9 · Cited by 200 · see citing works
McLaughlin, I., Dani, J. A., & De Biasi, M. (2015) · Current Topics in Behavioral Neurosciences · doi:10.1007/978-3-319-13482-6_4 · Cited by 173 · see citing works
“After at least a week of chronic nicotine administration in mice, cessation of nicotine exposure or administration of a nAChR antagonist induces affective, somatic, and cognitive signs of withdrawal (Malin et al. 1994; De Biasi and Salas 2008).”
BackgroundMalin, D. H. (2001) · Pharmacology Biochemistry and Behavior · doi:10.1016/s0091-3057(01)00699-2 · Cited by 167 · see citing works
Self-citationDiminished nicotine withdrawal in adolescent rats: implications for vulnerability to addiction
O’Dell, L. E., Bruijnzeel, A. W., Smith, R. T., Parsons, L. H., Merves, M. L., Goldberger, B. A., Richardson, H. N., Koob, G. F., & Markou, A. (2006) · Psychopharmacology · doi:10.1007/s00213-006-0383-6 · Cited by 153 · see citing works
Rada, P., Jensen, K., & Hoebel, B. (2001) · Psychopharmacology · doi:10.1007/s002130100781 · Cited by 141 · see citing works
Antidepressantlike effects of chronic nicotine on learned helplessness paradigm in rats
Semba, J., Mataki, C., Yamada, S., Nankai, M., & Toru, M. (1998) · Biological Psychiatry · doi:10.1016/s0006-3223(97)00477-0 · Cited by 140 · see citing works
Hildebrand, B. E., Nomikos, G. G., Bondjers, C., Nisell, M., & Svensson, T. H. (1997) · Psychopharmacology · doi:10.1007/s002130050200 · Cited by 122 · see citing works
Common aspects of the action of nicotine and other drugs of abuse
Picciotto, M. R. (1998) · Drug and Alcohol Dependence · doi:10.1016/s0376-8716(98)00074-x · Cited by 117 · see citing works
Nicotine regulates multiple synaptic proteins by inhibiting proteasomal activity
Rezvani, K., Teng, Y., Shim, D., & De Biasi, M. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.3353-07.2007 · Cited by 113 · see citing works
The Neuronal Pathways Mediating the Behavioral and Addictive Properties of Nicotine
Balfour, D. J. K. (2009) · Handbook of Experimental Pharmacology · doi:10.1007/978-3-540-69248-5_8 · Cited by 99 · see citing works
Carboni, E., Bortone, L., Giua, C., & Di Chiara, G. (2000) · Drug and Alcohol Dependence · doi:10.1016/s0376-8716(99)00064-2 · Cited by 96 · see citing works
Rodent models of nicotine withdrawal syndrome
Malin, D. H., & Goyarzu, P. (2009) · Handbook of experimental pharmacology · doi:10.1007/978-3-540-69248-5_14 · Cited by 89 · see citing works
Self-citationSalas, R., Main, A., Gangitano, D., & De Biasi, M. (2007) · Neuropharmacology · doi:10.1016/j.neuropharm.2007.08.017 · Cited by 76 · see citing works
Natividad, L. A., Tejeda, H. A., Torres, O. V., & O'dell, L. E. (2010) · Synapse · doi:10.1002/syn.20713 · Cited by 64 · see citing works
Shram, M. J., Siu, E. C. K., Li, Z., Tyndale, R. F., & Lê, A. D. (2008) · Psychopharmacology · doi:10.1007/s00213-008-1115-x · Cited by 60 · see citing works
Kallupi, M., de Guglielmo, G., Larrosa, E., & George, O. (2019) · European Neuropsychopharmacology · doi:10.1016/j.euroneuro.2019.08.299 · Cited by 25 · see citing works
Hamilton, K. R., Perry, M. E., Berger, S. S., & Grunberg, N. E. (2010) · Nicotine & Tobacco Research · doi:10.1093/ntr/ntq179 · Cited by 27 · see citing works
- Authors
- Malin, D. H., Ronald Lake, J., Carter, V. A., Scott Cunningham, J., Hebert, K. M., Conrad, D. L., & Wilson, O. B.
- Affiliations
- University of Houston - Clear Lake (US)
- Baylor College of Medicine (US)
- Venue
- Psychopharmacology
- Published
- 1994-06
- Volume
- 115
- Issue
- 1-2
- Pages
- 180-184
- ISSN
- 0033-3158 (ISSN-L)
- 1432-2072
- OpenAlex
- W1982978113
- Cited by
- 165
- References
- 34
- Citations by year
- 2025: 1 · 2024: 5 · 2022: 2 · 2021: 3 · 2020: 5 · 2019: 5 · 2018: 5 · 2017: 4 · 2016: 6 · 2015: 5 · 2014: 3 · 2013: 5 · 2012: 3
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Psychopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Smoking Behavior and Cessation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Substance Exposure Effects
- Keywords
- mecamylamine, nicotinic antagonists, nicotine dependence, rodent models, nicotinic acetylcholine receptors
- MeSH terms
- Animals, Behavior, Animal, Male, Mecamylamine, Nicotine, Substance Withdrawal Syndrome, Rats, Sprague-Dawley, Nicotinic Antagonists, Substance-Related Disorders, Rats
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