Cited by 754Aging and Gerontology ResearchGenetics, Aging, and Longevity in Model OrganismsT-cell and B-cell Immunology

The DOI matches its Crossref record; 8 of the 9 references it cites were found in a registry or an index.

Admission criteria for immunogerontological studies in man: The senieur protocol

Ligthart, Gerard J., Corberand, Joël X., Fournier, Catherine, Galanaud, Pierre, Hijmans, Willy, Kennes, Bernard, Müller-Hermelink, Hans K., Steinmann, Gerhard G.·1984·Mechanisms of Ageing and Development

DOI 10.1016/0047-6374(84)90152-0

Open this paper in CoChat — free account

Abstract

Immunogerontological studies in man have often led to conflicting results. One of the main reasons is the selection of the subjects to be studied. Admission criteria such as "apparently healthy" or "without overt disease" seem insufficient to exclude underlying disease which might influence the immune system and thereby the results. In an attempt to solve this problem, the SENIEUR protocol described in this paper was developed by a working party in the framework of the EURAGE Concerted Action Programme on Ageing of the European Community. This protocol establishes strict admission criteria for immunogerontological studies in man based on clinical information and laboratory data, and it sets limits to pharmacological interference. The use of this protocol will lead to standardization between centers and also to a closer study of the influence of age as such on the immune system. These findings in the immunologically "optimally aged" can also serve as reference values for immunogerontological studies in subjects who do not meet the SENIEUR criteria. In this way the use of this protocol can contribute to the dissection of the influence of disease versus ageing on the immune system.

Integrity signals

Registry

Crossref · 10.1016/0047-6374(84)90152-0

Cites

8 of 8 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Immunoglobulin patterns in humans over 95 years of age.
Radl, J., Sepers, J. M., Skvaril, F., Morell, A., & Hijmans, W. (1975) · PubMed Central · Cited by 146
Self-citation
Relationship between Aging and the Immune System
Kay, M. M. B., & Makinodan, T. (2015) · Chemical immunology/Fortschritte der Allergielehre/Progress in allergy/Chemical immunology and allergy · doi:10.1159/000400097 · Cited by 75
HISTOLOGY OF THE PROSTATE IN ELDERLY MEN
Harbitz, T. B., & Haugen, O. A. (1972) · Acta Pathologica Microbiologica Scandinavica Section A Pathology · doi:10.1111/j.1699-0463.1972.tb00347.x · Cited by 71
PHYTOHÆMAGGLUTININ-INDUCED LYMPHOCYTE TRANSFORMATION AND CIRCULATING AUTOANTIBODIES IN WOMEN TAKING ORAL CONTRACEPTIVES
Barnes, E. W., Loudon, N., MacCuish, A. C., Jordan, J., & Irvine, W. J. (1974) · The Lancet · doi:10.1016/s0140-6736(74)90348-1 · Cited by 70
Subpopulations of mononuclear cells in ageing: expansion of the null cell compartment and decrease in the number of T and B cells in human blood.
Ligthart, G. J., Schuit, H. R. E., & Hijmans, W. (1985) · PubMed Central · Cited by 64
Self-citation
Blood components in a 70-year-old population
Landahl, S., JAGENBURG, R., & Svanborg, A. (1981) · Clinica Chimica Acta · doi:10.1016/0009-8981(81)90453-8 · Cited by 56
Body fatness, relative weight and frame size in young adults
Baecke, J. A. H., BUREMA, J., & Deurenberg, P. (1982) · British Journal Of Nutrition · doi:10.1079/bjn19820081 · Cited by 44
Cellular Immunosenescence
Leech, S. H. (2009) · Gerontology · doi:10.1159/000212437 · Cited by 25

Show all 9 references

Research works printed but not linked: 3

Tezabwala, 1983, Studies on cell mediated immunity in women using different fertility regulatory methods, J. Clin. Lab. Immunol., 10, 199
de Viere, 1984, Immune senescence: I. Effect of age, sex and health state on human blood mononuclear subpopulations, Arch. Int. Gerontol.
Kennes, 1984, Immune senescence: II. Effect of age, sex and health state on human lymphocyte functions, Arch. Int. Gerontol.

Sorted by citations

Antibody response to influenza vaccination in the elderly: A quantitative review
GOODWIN, K., VIBOUD, C., & SIMONSEN, L. (2006) · Vaccine · doi:10.1016/j.vaccine.2005.08.105 · Cited by 1,064
The aging of the immune system
Weiskopf, D., Weinberger, B., & Grubeck-Loebenstein, B. (2009) · Transplant International · doi:10.1111/j.1432-2277.2009.00927.x · Cited by 908
Increased cytokine production in mononuclear cells of healthy elderly people
Fagiolo, U., Cossarizza, A., Scala, E., Fanales‐Belasio, E., Ortolani, C., Cozzi, E., Monti, D., Franceschi, C., & Paganelli, R. (1993) · European Journal of Immunology · doi:10.1002/eji.1830230950 · Cited by 665
Clinical Relevance of Age-Related Immune Dysfunction
Castle, S. C. (2000) · Clinical Infectious Diseases · doi:10.1086/313947 · Cited by 639
“One study of subjects who met the criteria of the SENIEUR protocol [2] (very healthy elderly) correlated impaired immune parameters with the prior inci-”
Influential
Age-related inflammatory cytokines and disease
Brüünsgaard, H., & Pedersen, B. K. (2003) · Immunology and Allergy Clinics of North America · doi:10.1016/s0889-8561(02)00056-5 · Cited by 608
“The SENIEUR protocol is used to separate the influence of morbidity and frailty from the physiologic process of aging [41].”
Method
Zinc and the immune system
Rink, L. (2000) · Proceedings of The Nutrition Society · doi:10.1017/s0029665100000781 · Cited by 598
“1982; Lighart et al .”
Aging and proinflammatory cytokines
Bruunsgaard, H., Pedersen, M., & Pedersen, B. K. (2001) · Current Opinion in Hematology · doi:10.1097/00062752-200105000-00001 · Cited by 690
Estrogen accelerates cutaneous wound healing associated with an increase in TGF-β1 levels
Ashcroft, G. S., Dodsworth, J., Boxtel, E. V., Tarnuzzer, R. W., Horan, M. A., Schultz, G. S., & Ferguson, M. W. J. (1997) · Nature Medicine · doi:10.1038/nm1197-1209 · Cited by 573
Innate Immune Function by Toll-like Receptors: Distinct Responses in Newborns and the Elderly
Kollmann, T. R., Levy, O., Montgomery, R. R., & Goriely, S. (2012) · Immunity · doi:10.1016/j.immuni.2012.10.014 · Cited by 591
“Should we try to boost the budding or the aging innate immune system, or would that augment a possibly negative impact, e.g., increase asthma and allergies in the young (Lisciandro and van den Biggelaar, 2010) or inflammaging in the elderly?”
BackgroundInfluential
Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response
Panda, A., Qian, F., Mohanty, S., van Duin, D., Newman, F. K., Zhang, L., Chen, S., Towle, V., Belshe, R. B., Fikrig, E., et al. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.0901022 · Cited by 548
“The older individuals in our cohort represented a largely healthy aged group, with nearly 80% reporting no major comorbid conditions; notably, we did not employ limiting enrollment criteria, such as SENIEUR, a protocol selecting for successfully aged individuals that excludes perhaps 80% of older individuals (35).”
Method
The immunology of exceptional individuals: the lesson of centenarians
Franceschi, C., Monti, D., Sansoni, P., & Cossarizza, A. (1995) · Immunology Today · doi:10.1016/0167-5699(95)80064-6 · Cited by 535
Human innate immunosenescence: causes and consequences for immunity in old age
Panda, A., Arjona, A., Sapey, E., Bai, F., Fikrig, E., Montgomery, R. R., Lord, J. M., & Shaw, A. C. (2009) · Trends in Immunology · doi:10.1016/j.it.2009.05.004 · Cited by 502
“For example, these studies relied upon different cell enrichment protocols, and variation in preparations of LPS might also have contributed to heterogeneity in results; in addition, some studies have limited enrollment using the highly restrictive SENIEUR protocol [11], while others have not.”
Result
The NLRP3 and NLRP1 inflammasomes are activated in Alzheimer’s disease
Saresella, M., La Rosa, F., Piancone, F., Zoppis, M., Marventano, I., Calabrese, E., Rainone, V., Nemni, R., Mancuso, R., & Clerici, M. (2016) · Molecular Neurodegeneration · doi:10.1186/s13024-016-0088-1 · Cited by 489
“Neurbiol Aging.”
MethodInfluential
Lymphocyte subsets and natural killer cell activity in healthy old people and centenarians [see comments]
Sansoni, P., Cossarizza, A., Brianti, V., Fagnoni, F. F., Snelli, G., Monti, D., Marcato, A., Passeri, G., Ortolani, C., & Forti, E. (1993) · Blood · doi:10.1182/blood.v82.9.2767.2767 · Cited by 457
The Involution of the Ageing Human Thymic Epithelium is Independent of Puberty
Steinmann, G. G., KLAUS, B. M., & Müller-Hermelink, H. K. (1985) · Scandinavian Journal of Immunology · doi:10.1111/j.1365-3083.1985.tb01916.x · Cited by 455
Self-citation
Senescence in innate immune responses: reduced neutrophil phagocytic capacity and CD16 expression in elderly humans
Butcher, S. K., Chahal, H., Nayak, L., Sinclair, A., Henriquez, N. V., Sapey, E., O'Mahony, D., & Lord, J. M. (2001) · Journal of Leukocyte Biology · doi:10.1189/jlb.70.6.881 · Cited by 436
“immunogerontological criteria of the SENIEUR protocol [16] were used in the”
Method
Topical Estrogen Accelerates Cutaneous Wound Healing in Aged Humans Associated with an Altered Inflammatory Response
Ashcroft, G. S., Greenwell-Wild, T., Horan, M. A., Wahl, S. M., & Ferguson, M. W. J. (1999) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)65217-0 · Cited by 420
Age-dependent alterations of monocyte subsets and monocyte-related chemokine pathways in healthy adults
Seidler, S., Zimmermann, H. W., Bartneck, M., Trautwein, C., & Tacke, F. (2010) · BMC Immunology · doi:10.1186/1471-2172-11-30 · Cited by 390
“Using these efforts, all participants, including the persons above 65 years of age, could be considered "healthy" based on admission criteria of the extensively validated 'senieur protocol' [17,18].”
Altered cytokine production in the elderly
Rink, L., Cakman, I., & Kirchner, H. (1998) · Mechanisms of Ageing and Development · doi:10.1016/s0047-6374(97)00153-x · Cited by 378
T cells and aging january 2002 update
Pawelec, G. (2002) · Frontiers in bioscience · doi:10.2741/a831 · Cited by 372
Age-Associated Defect in Human TLR-1/2 Function
van Duin, D., Mohanty, S., Thomas, V., Ginter, S., Montgomery, R. R., Fikrig, E., Allore, H. G., Medzhitov, R., & Shaw, A. C. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.178.2.970 · Cited by 352
“Consistent with this approach, we did not choose to apply restrictive enrollment criteria, such as the SENIEUR protocol, which is based on clinical data and laboratory measurements that typically result in the exclusion of 70% of subjects older than 65 years of age (23, 24).”
Method
T cells and aging
Pawelec, G. (1998) · Frontiers in bioscience · doi:10.2741/a266 · Cited by 357
“It was therefore anticipated that when data became available using donors selected according to standard criteria (namely, the SENIEUR protocol (127)), results on cytokine secretion and other immunogerontological parameters would become clearer.”
Aging and innate immune cells
Plackett, T. P., Boehmer, E. D., Faunce, D. E., & Kovacs, E. J. (2004) · Journal of Leukocyte Biology · doi:10.1189/jlb.1103592 · Cited by 351
“The SENIEUR protocol, which designates young as 25–34 years old and aged as 65 and above, is not exclusively used as a result of its limitations [8].”
Background
The Cytokine Response to Physical Activity and Training
Moldoveanu, A., Shephard, R., & Shek, P. (2001) · Sports Medicine · doi:10.2165/00007256-200131020-00004 · Cited by 339
“[150] determined that, compared with young individuals, the elderly (mean age 74 years) had significantly elevated IL-6, TNFα and IL-1β levels in the supernatants of mitogenstimulated cultures.”
Result
Major Depressive Disorder and Immunity
Schleifer, S. J. (1989) · Archives of General Psychiatry · doi:10.1001/archpsyc.1989.01810010083011 · Cited by 293
Markers to measure immunomodulation in human nutrition intervention studies
Albers, R., Antoine, J., Bourdet‐Sicard, R., Calder, P. C., Gleeson, M., Lesourd, B., Samartı́n, S., Sanderson, I. R., Loo, J. V., Dias, F. W. V., et al. (2005) · British Journal Of Nutrition · doi:10.1079/bjn20051469 · Cited by 284
“Both changes affect T cell functions with a progressive decline in both lymphocyte proliferation and IL-2 synthesis, which are strongly associated with higher prevalence of infectious diseases in most elderly persons, although those selected for extreme good health (e.g. using the SENIEUR protocol; Ligthart et al. 198…”
Background
Nutrition and immunity in the elderly: modification of immune responses with nutritional treatments
Lesourd, B. (1997) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/66.2.478s · Cited by 285
Peripheral blood dendritic cells and monocytes are differently regulated in the elderly
Bella, S. D., Bierti, L., Presicce, P., Arienti, R., Valenti, M., Saresella, M., Vergani, C., & Villa, M. L. (2006) · Clinical Immunology · doi:10.1016/j.clim.2006.09.012 · Cited by 280
Zinc-Altered Immune Function and Cytokine Production
Rink, L., & Kirchner, H. (2000) · Journal of Nutrition · doi:10.1093/jn/130.5.1407s · Cited by 274
Age-dependent decrease in Toll-like receptor 4-mediated proinflammatory cytokine production and mitogen-activated protein kinase expression
Boehmer, E. D., Góral, J., Faunce, D. E., & Kovacs, E. J. (2003) · Journal of Leukocyte Biology · doi:10.1189/jlb.0803389 · Cited by 266
“Earlier studies that indicated elevated inflammatory cytokines in aging humans used the SENIEUR protocol to exclude unhealthy subjects [42, 43].”
Background
The network and the remodeling theories of aging: historical background and new perspectives
Franceschi, C., Valensin, S., Bonafè, M., Paolisso, G., Yashin, A. I., Monti, D., & De Benedictis, G. (2000) · Experimental Gerontology · doi:10.1016/s0531-5565(00)00172-8 · Cited by 335
Hypercoagulability in centenarians: the paradox of successful aging
Mari, D., Mannucci, P. M., Coppola, R., Bottasso, B., Bauer, K. A., & ROSENBERG, R. D. (1995) · Blood · doi:10.1182/blood.v85.11.3144.bloodjournal85113144 · Cited by 257
CD45 isoforms expression on CD4+ and CD8+ T cells throughout life, from newborns to centenarians: implications for T cell memory
Cossarizza, A., Ortolani, C., Paganelli, R., Barbieri, D., Monti, D., Sansoni, P., Fagiolo, U., Castellani, G., Bersani, F., Londei, M., et al. (1996) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(95)01691-0 · Cited by 253
Plasma cytokine profiles in elderly humans
Forsey, R. J., Thompson, J. M., Ernerudh, J., HURST, T. L., Strindhall, J., Johansson, B. E. A., Nilsson, B.-O., & Wikby, A. (2003) · Mechanisms of Ageing and Development · doi:10.1016/s0047-6374(03)00025-3 · Cited by 249
The aging of the immune system
Grubeck-Loebenstein, B., & Wick, G. (2002) · Advances in immunology · doi:10.1016/s0065-2776(02)80017-7 · Cited by 233
Complex alteration of thyroid function in healthy centenarians.
Mariotti, S., Barbesino, G., Caturegli, P., Bartalena, L., Sansoni, P., Fagnoni, F., Monti, D., Fagiolo, U., Franceschi, C., & Pinchera, A. (1993) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.77.5.8077303 · Cited by 234
Exercise Studies with Elderly Volunteers
Greig, C. A., Young, A., Skelton, D. A., PIPPET, E., Butler, F., & MAHMUD, S. M. (1994) · Age and Ageing · doi:10.1093/ageing/23.3.185 · Cited by 229
“The SENIEUR protocol [6, 7] was developed to identify healthy subjects for immunological studies but is not ideal for exercise studies.”
Method
Vaccination in the elderly: an immunological perspective
Chen, W. H., Kozlovsky, B. F., Effros, R. B., Grubeck‐Loebenstein, B., Edelman, R., & Sztein, M. B. (2009) · Trends in Immunology · doi:10.1016/j.it.2009.05.002 · Cited by 228
Age-related changes in natural killer cell receptors from childhood through old age
Almeida-Oliveira, A., Smith-Carvalho, M., Pôrto, L. C., Cardoso-Oliveira, J., Ribeiro, A. D. S., Falcão, R. R., Abdelhay, E., Bouzas, L. F., Thuler, L. C. S., Ornellas, M. H., et al. (2011) · Human Immunology · doi:10.1016/j.humimm.2011.01.009 · Cited by 223
Aging of immune system: immune signature from peripheral blood lymphocyte subsets in 1068 healthy adults
Qin, L., Xie, J., Qiu, Z., Cao, W., Jiao, Y., Routy, J., & Li, T. (2016) · Aging · doi:10.18632/aging.100894 · Cited by 218
“The recruitment was strictly conducted according to the defined criteria from the SENIEUR protocol guideline [39, 40].”
Method
Innate and adaptive immunosenescence
Agarwal, S., & Busse, P. J. (2010) · Annals of Allergy Asthma & Immunology · doi:10.1016/j.anai.2009.11.009 · Cited by 215
Aging of the Immune System: Focus on Natural Killer Cells Phenotype and Functions
Brauning, A., Rae, M., Zhu, G., Fulton, E., Admasu, T. D., Stolzing, A., & Sharma, A. (2022) · Cells · doi:10.3390/cells11061017 · Cited by 215
“One study demonstrated a decreased sensitivity of NK cells to IL-2 when derived from older donors [62], which may at least in part be explained by the use of the SENIEUR patient selection criteria in this study, which attempted to avoid confounding factors that elicit strong immunological responses, such as current in…”
Result
Dysregulation of human Toll-like receptor function in aging
Shaw, A. C., Panda, A., Joshi, S. R., Qian, F., Allore, H. G., & Montgomery, R. R. (2011) · Ageing Research Reviews · doi:10.1016/j.arr.2010.10.007 · Cited by 215
“in genetically defined murine systems), but also heterogeneity in infectious exposures, medication use, comorbid medical conditions and other factors that accrue during a lifespan? One approach is a restrictive enrollment procedure such as the SENIEUR Protocol, which was developed in 1984 by the working party of the E…”
Association between natural killer cell activity and infection in immunologically normal elderly people
Ogata, K., An, E., Shioi, Y., Nakamura, K., Luo, S., Yokose, N., Minami, S., & Dan, K. (2001) · Clinical & Experimental Immunology · doi:10.1046/j.1365-2249.2001.01571.x · Cited by 207
Age-Associated B Cells with Proinflammatory Characteristics Are Expanded in a Proportion of Multiple Sclerosis Patients
Claes, N., Fraussen, J., Vanheusden, M., Hellings, N., Stinissen, P., Wijmeersch, B. V., Hupperts, R., & Somers, V. A. (2016) · The Journal of Immunology · doi:10.4049/jimmunol.1502448 · Cited by 206
The Immune System in the Elderly: Activation-Induced and Damage-Induced Apoptosis
Ginaldi, L., Martinis, M. D., Monti, D., & Franceschi, C. (2004) · Immunologic Research · doi:10.1385/ir:30:1:081 · Cited by 202
Ageing and the neutrophil: no appetite for killing?
Butcher, S. K., Chahel, H., & Lord, J. M. (2000) · Immunology · doi:10.1046/j.1365-2567.2000.00079.x · Cited by 200
The six-minute walk test in community dwelling elderly: influence of health status.
Bautmans, I., Lambert, M., & Mets, T. F. (2004) · BMC Geriatrics · doi:10.1186/1471-2318-4-6 · Cited by 212
“In order to distinguish completely healthy participants from those with a worse health status we used criteria derived from the SENIEUR protocol [22].”
MethodInfluential
Blood pressure and mortality in elderly people aged 85 and older: community based study
Boshuizen, H. C., Izaks, G. J., van Buuren, S., & Ligthart, G. J. (1998) · BMJ · doi:10.1136/bmj.316.7147.1780 · Cited by 196
Self-citation
Influence of Physical Activity on Serum IL-6 and IL-10 Levels in Healthy Older Men
Jankord, R., & Jemioło, B. (2004) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000128186.09416.18 · Cited by 196
“Acceptable volunteers met the clinical and pharmacological requirements of the SENIEUR protocol and were 1) without acute or chronic diseases: 2) nonsmokers (at least for last 2 yr): and 3) without infection, inflammation, or malignancy (11).”
Method
Antibody induction by influenza vaccines in the elderly: a review of the literature
BEYER, W. E. P., Palache, A. M., Baljet, M., & Masurel, N. (1989) · Vaccine · doi:10.1016/0264-410x(89)90150-3 · Cited by 194
Circulating, Interferon‐Producing Plasmacytoid Dendritic Cells Decline During Human Ageing
Shodell, M., & Siegal, F. P. (2002) · Scandinavian Journal of Immunology · doi:10.1046/j.1365-3083.2002.01148.x · Cited by 189
Increased activity of Th-17 and Th-9 lymphocytes and a skewing of the post-thymic differentiation pathway are seen in Alzheimer’s disease
Saresella, M., Calabrese, E., Marventano, I., Piancone, F., Gatti, A. V., Alberoni, M., Nemni, R., & Clerici, M. (2010) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2010.12.004 · Cited by 194
Decreased TNF-α synthesis by macrophages restricts cutaneous immunosurveillance by memory CD4+ T cells during aging
Agius, E., Lacy, K. E., Vukmanovic‐Stejic, M., Jagger, A. L., Papageorgiou, A., Hall, S., Reed, J. R., Curnow, J., Fuentes‐Duculan, J., Buckley, C. D., et al. (2009) · The Journal of Experimental Medicine · doi:10.1084/jem.20090896 · Cited by 190
“Exclusion criteria based on a modified version of the SENIEUR protocol were used to recruit old people to reduce confounding factors caused by associated significant comorbidity (Ligthart et al., 1984).”
Method
Effect of micronutrient status on natural killer cell immune function in healthy free-living subjects aged ≥90 y
Ravaglia, G., Forti, P., Maioli, F., Bastagli, L., Facchini, A., Mariani, E., Savarino, L., Sassi, S., Cucinotta, D., & Lenaz, G. (2000) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/71.2.590 · Cited by 189
Age-related differences in the temporal and spatial regulation of matrix metalloproteinases (MMPs) in normal skin and acute cutaneous wounds of healthy humans
Ashcroft, G. S., Horan, M. A., Herrick, S. E., Tarnuzzer, R. W., Schultz, G. S., & Ferguson, M. W. J. (1997) · Cell and Tissue Research · doi:10.1007/s004410050963 · Cited by 188
“One hundred and thirty-two Caucasian health-status-defined subjects of both sexes (screened by the SENIEUR protocol; Ligthart et al. 1984) aged between 19 years and 96 years of age were divided into three age-groups (19–39 years, young; 40–59 years, middle-aged; 60+ years, old) with equal numbers of males and females…”
Background
Dysregulation between TH1 and TH2 T cell subpopulations in the elderly
Cakman, I., Rohwer, J., Schütz, R.-M., Kirchner, H., & Rink, L. (1996) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(96)01708-3 · Cited by 184
Inadequately low serum levels of steroid hormones in relation to interleukin‐6 and tumor necrosis factor in untreated patients with early rheumatoid arthritis and reactive arthritis
Straub, R. H., Paimela, L., Peltomaa, R., Schölmerich, J., & Leirisalo‐Repo, M. (2002) · Arthritis & Rheumatism · doi:10.1002/art.10177 · Cited by 183
Stress, glucocorticoids and ageing of the immune system
Bauer, M. E. (2005) · Stress · doi:10.1080/10253890500100240 · Cited by 191
“Therefore, strenuous efforts have been made to circumvent this problem by separating “disease” from “ageing”, as exemplified by the application of the SENIEUR protocol (Ligthart et al. 1984), which defines rigorous criteria for selecting healthy individuals in immunogerontological studies.”
Background
Mitochondrial DNA polymorphism: its role in longevity of the Irish population
Ross, O. A. (2001) · Experimental Gerontology · doi:10.1016/s0531-5565(01)00094-8 · Cited by 181
Lipoprotein(a) and lipoprotein profile in healthy centenarians: a reappraisal of vascular risk factors
Baggio, G., Donazzan, S., Monti, D., Mari, D., Martini, S., Gabelli, C., Vestra, M. D., Previato, L., Guido, M., Pigozzo, S., et al. (1998) · The FASEB Journal · doi:10.1096/fasebj.12.6.433 · Cited by 178
“Group 4 included old volunteers carefully selected according to the SENIEUR Protocol proposed by Ligthart et al. ( 13 ).”
Method
Necessity of the assessment of health status in human immunogerontological studies: Evaluation of the senieur protocol
Ligthart, G. J., Corberand, J. X., Geertzen, H. G. M., Meinders, A. E., Knook, D. L., & Hijmans, W. (1990) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(90)90108-r · Cited by 179
Self-citation
Cytokine production and lymphocyte subpopulations in aged humans. An assessment during nocturnal sleep
Jan Born, A., Uthgenannt, D., Dodt, C., Nünninghoff, D., Ringvolt, E., Wagner, T., & Fehm, H. (1995) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(95)01638-4 · Cited by 174
Age-related modifications of the human alphabeta T cell repertoire due to different clonal expansions in the CD4+ and CD8+ subsets
Wack, A. (1998) · International Immunology · doi:10.1093/intimm/10.9.1281 · Cited by 173
Serum heat shock protein and anti-heat shock protein antibody levels in aging
Rea, I. M., McNerlan, S., & Pockley, A. G. (2001) · Experimental Gerontology · doi:10.1016/s0531-5565(00)00215-1 · Cited by 171
Measurement of Thirteen Biological Markers in CSF of Patients with Alzheimer’s Disease and Other Dementias
Blasko, I., Lederer, W., OBERBAUER, H., Walch, T., Kemmler, G., Hinterhuber, H., Marksteiner, J., & Humpel, C. (2005) · Dementia and Geriatric Cognitive Disorders · doi:10.1159/000089137 · Cited by 172
“ROC curves can be used to determine cut-off values for a given sensitivity or specificity level. Cut-off values were based on a maximal area under the curve. They were further used to determine the cut-off that maximizes the sum of sensitivity and specificity. Results Study Population The mean age was not significantl…”
Influential
Increased number of circulating Leu 11+ (CD 16) large granular lymphocytes and decreased NK activity during human ageing.
Facchini, A., Mariani, E., Mariani, A. R., Papa, S., Vitale, M., & Manzoli, F. A. (1987) · PubMed Central · Cited by 170
Physical exercise and immune system function in cancer survivors
Fairey, A., Courneya, K. S., Field, C. J., & Mackey, J. R. (2002) · Cancer · doi:10.1002/cncr.10244 · Cited by 164
“Importantly, research has shown that several components of the immune system are significantly influenced by such variables.(44,45) For instance, increasing age has been associated with decreases in the total number of peripheral blood lymphocytes and T cells (CD4 , CD8 ), T cell (CD45RA ) function, T cell responses t…”
Background
Impact of aging on innate immunity
Pawelec, G., Solana, R., Remarque, E. J., & Mariani, E. (1998) · Journal of Leukocyte Biology · doi:10.1002/jlb.64.6.703 · Cited by 163
Prevaccine Determination of the Expression of Costimulatory B7 Molecules in Activated Monocytes Predicts Influenza Vaccine Responses in Young and Older Adults
van Duin, D., Allore, H. G., Mohanty, S., Ginter, S., Newman, F. K., Belshe, R. B., Medzhitov, R., & Shaw, A. C. (2007) · The Journal of Infectious Diseases · doi:10.1086/516788 · Cited by 159
“In the present study, we chose not to employ a restrictive enrollment protocol such as SENIEUR, which utilizes clinical and laboratory data that results in the exclusion 170% of subjects 165 years of age [35, 36].”
The relationship of dehydroepiandrosterone sulfate (DHEAS) to endocrine- metabolic parameters and functional status in the oldest-old. Results from an Italian study on healthy free-living over-ninety-year-olds
Ravaglia, G., Forti, P., Maioli, F., Boschi, F., Bernardi, M., Pratelli, L., Pizzoferrato, A., & Gasbarrini, G. (1996) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.81.3.8772596 · Cited by 153
Age‐Related Immune Dysfunction in Health and in Human Immunodeficiency Virus (HIV) Disease: Association of Age and HIV Infection with Naive CD8+Cell Depletion, Reduced Expression of CD28 on CD8+Cells, and Reduced Thymic Volumes
Kalayjian, R. C., LANDAY, A. L., Pollard, R. B., Taub, D. D., Gross, B. H., Francis, I. R., Sevin, A. D., Pu, M., Spritzler, J., Chernoff, M., et al. (2003) · The Journal of Infectious Diseases · doi:10.1086/375372 · Cited by 158
Impaired production of proinflammatory cytokines in response to lipopolysaccharide (LPS) stimulation in elderly humans
Brüünsgaard, H., Pedersen, B. K., Schroll, M., Skinhøj, P., & Pedersen, B. K. (1999) · Clinical & Experimental Immunology · doi:10.1046/j.1365-2249.1999.01045.x · Cited by 158
Changes in circuIating B cells and immunoglobulin cIasses and subcIasses in a healthy aged popuIation
Paganelli, R., Quinti, I., Fagiolo, U., Cossarizza, A., ORTOLANI, C., GUERRA, E., Sansoni, P., Pucillo, L. P., Scala, E., Cozzi, E., et al. (1992) · Clinical & Experimental Immunology · doi:10.1111/j.1365-2249.1992.tb07954.x · Cited by 156
Nutrition and immunity in the elderly
Lesourd, B., & Mazari, L. (1999) · Proceedings of The Nutrition Society · doi:10.1017/s0029665199000907 · Cited by 154
“‡ Young adults (25–34 years) and young elderly (75–84 years) were selected according to the SENIEUR protocol (Ligthart et al. 1984) plus additional criteria (Lesourd et al. 1994; Lesourd & Mearne, 1994).”
Influential
Diseases of aging
Wick, G., Jansen‐Dürr, P., Berger, P., Blasko, I., & Grubeck‐Loebenstein, B. (2000) · Vaccine · doi:10.1016/s0264-410x(99)00489-2 · Cited by 152
“The SENIEUR-Protocol is used to de®ne ``healthy old'' probands and the respective young controls by precise information on their living habits (must be self-sustaining), laboratory values and drug intake [11,12].”
Method
Natural Killer Cells in the Late Decades of Human Life
Ogata, K., Yokose, N., Tamura, H., An, E., Nakamura, K., Dan, K., & Nomura, T. (1997) · Clinical Immunology and Immunopathology · doi:10.1006/clin.1997.4401 · Cited by 145
PD1 Negative and PD1 Positive CD4+ T Regulatory Cells in Mild Cognitive Impairment and Alzheimer's Disease
Saresella, M., Calabrese, E., Marventano, I., Piancone, F., Gatti, A. V., Calvo, M. G., Nemni, R., & Clerici, M. (2010) · Journal of Alzheimer s Disease · doi:10.3233/jad-2010-091696 · Cited by 146
“Healthy elderly human groups were selected in according to the SENIEUR protocol for immuno-gerontological studies of European Community’s Control Action Programme on Aging [9,32].”
Aging and its impact on innate immunity and inflammation: Implications for periodontitis
Hajishengallis, G. (2013) · Journal of Oral Biosciences · doi:10.1016/j.job.2013.09.001 · Cited by 150
“This protocol established rigorous enrollment criteria for immunogerontological studies, on the basis of clinical and laboratory data, and set limits to pharmacological interference [25].”
Daily micronutrient supplements enhance delayed-hypersensitivity skin test responses in older people
Bogden, J. D., Bendich, A., Kemp, F., Bruening, K. S., Shurnick, J., Denny, T. N., Baker, H., & Louria, D. B. (1994) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/60.3.437 · Cited by 144
“mission Criteria for Immunogeronological Studies in Man: The Senieur Protocol’ ‘ ( 20 ).”
Background
Cytometric analysis of immunosenescence
Cossarizza, A., Ortolani, C., Monti, D., & Franceschi, C. (1997) · Cytometry · doi:10.1002/(sici)1097-0320(19970401)27:4 · Cited by 142
The aging immune system: Primary and secondary alterations of immune reactivity in the elderly
Wick, G., & Grubeck‐Loebenstein, B. (1997) · Experimental Gerontology · doi:10.1016/s0531-5565(96)00152-0 · Cited by 142
Changes in apoptosis of human polymorphonuclear granulocytes with aging
Fülöp Jr, T., Fouquet, C., Allaire, P., Perrin, N., Lacombe, G., Stankova, J., Rola-Pleszczynski, M., Gagné, D., Wagner, J. R., Khalil, A., et al. (1997) · Mechanisms of Ageing and Development · doi:10.1016/s0047-6374(96)01881-7 · Cited by 141
Effect of 50- and 100-mg vitamin E supplements on cellular immune function in noninstitutionalized elderly persons
Pallast, E. G., Schouten, E. G., de Waart, F. G., Fonk, H. C., Doekes, G., von Blomberg, B. M., & Kok, F. J. (1999) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/69.6.1273 · Cited by 141
“To avoid distortion by concomitant chronic diseases or infectious diseases occurring during intervention, analyses were also carried out in a healthy, so-called “senieur” population that was selected according to the SENIEUR protocol of the working party of the EURAGE Concerted Action Programme on Ageing of the Europe…”
Method
Vaccine-induced antibody responses as parameters of the influence of endogenous and environmental factors.
Loveren, H. V., van Amsterdam, J., Vandebriel, R. J., Kimman, T. G., Rümke, H. C., Steerenberg, P. S., & Vos, J. G. (2001) · Environmental Health Perspectives · doi:10.1289/ehp.01109757 · Cited by 141
T cells and aging (update February 1999)
Pawelec, G. (1999) · Frontiers in bioscience · doi:10.2741/pawelec · Cited by 140
“It was nonetheless anticipated that when data became available using donors selected according to the strict standard criteria of the SENIEUR protocol (207)), results on cytokine secretion and other immunogerontological parameters would become more reproducible.”
Dietary intake and biochemical indices of nutritional status in an elderly population, with estimates of the precision of the 7-d food record
Payette, H., & Gray‐Donald, K. (1991) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/54.3.478 · Cited by 138
Aging and the Skin Immune System
Sunderkötter, C. H. (1997) · Archives of Dermatology · doi:10.1001/archderm.1997.03890460078009 · Cited by 133
Serum concentrations of zinc and selenium in elderly people: results in healthy nonagenarians/centenarians
Savarino, L., Granchi, D., Ciapetti, G., Cenni, E., Ravaglia, G., Forti, P., Maioli, F., & Mattioli, R. (2001) · Experimental Gerontology · doi:10.1016/s0531-5565(00)00218-7 · Cited by 133
“Subjects were selected as reported in a previous research (Ravaglia et al., 1997) following the suggestions of the SENIEUR protocol admission criteria (Ligthart et al., 1984).”
Toll‐Like Receptors in Older Adults
van Duin, D., & Shaw, A. C. (2007) · Journal of the American Geriatrics Society · doi:10.1111/j.1532-5415.2007.01300.x · Cited by 131
“This highly restrictive protocol typically results in the exclusion of more than 70% of communitydwelling individuals aged 65 and older.(43,44) Although SENIEUR was developed in an attempt to standardize studies on human aging (albeit restricting such studies to the subset of the successfully aged), no agreement on al…”
BackgroundMethod
CD69, CD25, and HLA-DR activation antigen expression on CD3+ lymphocytes and relationship to serum TNF-α, IFN-γ, and sIL-2R levels in aging
Rea, I. M., McNerlan, S., & Alexander, H. D. (1999) · Experimental Gerontology · doi:10.1016/s0531-5565(98)00058-8 · Cited by 129
Changes in serum cytokine concentrations during the menopausal transition
Yasui, T., Maegawa, M., Tomita, J., Miyatani, Y., Yamada, M., Uemura, H., Matsuzaki, T., Kuwahara, A., Kamada, M., Tsuchiya, N., et al. (2006) · Maturitas · doi:10.1016/j.maturitas.2006.11.002 · Cited by 129
The immune system in the elderly
Ginaldi, L., Martinis, M. D., D’Ostilio, A., Marini, L., Loreto, M. F., & Quaglino, D. (1999) · Immunologic Research · doi:10.1007/bf02786468 · Cited by 129
The frail elderly: role of dendritic cells in the susceptibility of infection
Uyemura, K., Castle, S. C., & Makinodan, T. (2002) · Mechanisms of Ageing and Development · doi:10.1016/s0047-6374(02)00033-7 · Cited by 127
Tumour necrosis factor-α-induced migration of human Langerhans cells: the influence of ageing: TNF-Α and LANGERHANS CELLS
Bhushan, M., Cumberbatch, M., Dearman, R. J., Andrew, S. M., Kimber, I., & Griffiths, C. E. M. (2002) · British Journal of Dermatology · doi:10.1046/j.1365-2133.2002.04549.x · Cited by 126
Aging of the Innate Immune System: An Update
Mahbub, S., Brubaker, A. L., & Kovacs, E. J. (2011) · Current Immunology Reviews · doi:10.2174/157339511794474181 · Cited by 128
“Another restriction could be due to the fact that some studies have limited enrollment as they followed the SENIEUR protocol [60].”
Background
Vitamin E ingestion improves several immune functions in elderly men and women
De la Fuente, M., Hernanz, A., Guayerbas, N., Manuel Victor, V., & Arnalich, F. (2008) · Free Radical Research · doi:10.1080/10715760801898838 · Cited by 124
“All elderly subjects were selected according to the immunogerontological ‘SENIEUR’ protocol [34].”
Method
The significance of zinc for leukocyte biology
Wellinghausen, N., & Rink, L. (1998) · Journal of Leukocyte Biology · doi:10.1002/jlb.64.5.571 · Cited by 123
“SENIEUR protocol [93]), impaired immunological functions”
Zinc supplementation reconstitutes the production of interferon-α by leukocytes from elderly persons
CAKMAN, I., KIRCHNER, H., & RINK, L. (1997) · Journal of Interferon & Cytokine Research · doi:10.1089/jir.1997.17.469 · Cited by 121
A Complex Proinflammatory Role for Peripheral Monocytes in Alzheimer's Disease
Saresella, M., Marventano, I., Calabrese, E., Piancone, F., Rainone, V., Gatti, A. V., Alberoni, M., Nemni, R., & Clerici, M. (2013) · Journal of Alzheimer s Disease · doi:10.3233/jad-131160 · Cited by 124
“0HandheldAutomatedCellCounter cultures 1 × 10 6 PBMC were cultured for 24 h in RPMI 1640 supplemented with 10% human”

Authors: Ligthart, G. J., Corberand, J. X., Fournier, C., Galanaud, P., Hijmans, W., Kennes, B., Müller-Hermelink, H. K., & Steinmann, G. G.
Affiliations: Leiden University (NL)
Hôpital Necker-Enfants Malades (FR)
Université Paris-Sud (FR)
Christian-Albrechts-Universität zu Kiel (DE)
Corresponding author: Ligthart, Gerard J.
ORCID: Galanaud, Pierre
Venue: Mechanisms of Ageing and Development
Publisher: Elsevier BV
Published: 1984-11
Volume: 28
Issue: 1
Pages: 47-55
ISSN: 0047-6374 (ISSN-L)
DOI: 10.1016/0047-6374(84)90152-0
OpenAlex: W2037412303
Cited by: 754
References: 9
Citations by year: 2026: 2 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 6 · 2021: 9 · 2020: 10 · 2019: 11 · 2018: 13 · 2017: 8 · 2016: 17 · 2015: 11 · 2014: 11 · 2013: 22 · 2012: 18
FWCI: 13.79
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Mechanisms of Ageing and Development (publishedVersion)
PubMed (publishedVersion)
Topics: Social Sciences › Psychology › Developmental and Educational Psychology › Aging and Gerontology Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords: healthy aging, reference values, age-related immune changes
MeSH terms: Adult, Aged, Aging, Cardiovascular Diseases, Humans, Immunity, Infections, Inflammation, Lymphoproliferative Disorders, Neoplasms, Research Design

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Admission criteria for immunogerontological studies in man: The senieur protocol

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Citations

Cites

Cited by

Related

Metadata

Record

About these checks