The DOI matches its Crossref record; 42 of the 45 references it cites were found in a registry or an index.
Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells
Abstract
apoE-deficient mice have been created by homologous recombination in ES cells. On a low fat, low cholesterol chow diet these animals have plasma cholesterol levels of 494 mg/dl compared with 60 mg/dl in control animals, and when challenged with a high fat Western-type diet, these animals have plasma cholesterol levels of 1821 mg/dl compared with 132 mg/dl in controls. This marked hypercholesterolemia is primarily due to elevated levels of very low and intermediate density lipoproteins. At 10 weeks of age, apoE-deficient mice have already developed atherosclerotic lesions in the aorta and coronary and pulmonary arteries. apoE-deficient mice are a promising small animal model to help understand the role of apoE in vivo and the genetic and environmental determinants of atherosclerosis.
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In the citing paper
“The increased amounts of apoA-IV in VLDL and IDL we observed were seen in other reports of apoE deficiency4,5,9 and in apoE-deficient mice.31”
From Effects of the Absence of Apolipoprotein E on Lipoproteins, Neurocognitive Function, and Retinal Function (2014) · cites this paper as [31]
In the citing paper
“Increased amounts of apoA-I were observed in LDL from an apoE-deficient patient9 and in VLDL from ApoE knockout mice.31”
From Effects of the Absence of Apolipoprotein E on Lipoproteins, Neurocognitive Function, and Retinal Function (2014) · cites this paper as [31]
In the citing paper
“Disorders of apoE are associated with atherosclerosis, which causes dyslipidemia and myocardial infarction, and apoE-deficient mice are used as model animals for atherosclerosis2, 3).”
From Impact of Apolipoprotein E Variants: A Review of Naturally Occurring Variants and Clinical Features (2025) · cites this paper as [3]
In the citing paper
“Disorders of apoE are associated with atherosclerosis, which causes dyslipidemia and myocardial infarction, and apoE-deficient mice are used as model animals for atherosclerosis2, 3).”
From Impact of Apolipoprotein E Variants: A Review of Naturally Occurring Variants and Clinical Features (2025) · cites this paper as [3]
In the citing paper
“In vivo and in vitro studies have shown that mutations in apoE that diminish binding of apoE-containing lipoproteins to the LDL receptor are associated with high plasma cholesterol levels and cause premature atherosclerosis in humans and experimental animals ( 1-3, 7, 8 ).”
From Molecular etiology of a dominant form of type III hyperlipoproteinemia caused by R142C substitution in apoE4 (2011) · cites this paper as [1]
Citations
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Evidence for differential effects of apoE3 and apoE4 on HDL metabolism
Hopkins, P. C. R., Huang, Y., McGuire, J. G., & Pitas, R. E. (2002) · Journal of Lipid Research · doi:10.1194/jlr.m200172-jlr200 · Cited by 31
Genomics and Cardiovascular Drug Development
Plump, A. S., & Lum, P. Y. (2009) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2008.11.050 · Cited by 31
Self-citationGeorgiadou, D., Chroni, A., Vezeridis, A., Zannis, V. I., & Stratikos, E. (2011) · PLoS ONE · doi:10.1371/journal.pone.0027037 · Cited by 24
Impact of Apolipoprotein E Variants: A Review of Naturally Occurring Variants and Clinical Features
Matsunaga, A., & Saito, T. (2025) · Journal of Atherosclerosis and Thrombosis · doi:10.5551/jat.65393 · Cited by 14
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Vezeridis, A. M., Drosatos, K., & Zannis, V. I. (2011) · Journal of Lipid Research · doi:10.1194/jlr.m008409 · Cited by 11
Hepatic Lipoprotein Metabolism: Current and Future In Vitro Cell-Based Systems
Kiss, I., Neuwert, N., Oberle, R., Hengstschläger, M., Osmanagic-Myers, S., & Stangl, H. (2025) · Biomolecules · doi:10.3390/biom15070956 · Cited by 11
Pathophysiology of ischaemia-reperfusion injury
Carden, D. L., & Granger, D. N. (2000) · The Journal of Pathology · doi:10.1002/(sici)1096-9896(200002)190:3 · Cited by 5
Fotakis, P., Vezeridis, A., Dafnis, I., Chroni, A., Kardassis, D., & Zannis, V. I. (2014) · Journal of Lipid Research · doi:10.1194/jlr.m048348 · Cited by 4
Cole (2000) · doi:10.1002/1097-0029(20000815)50:4
Metadata
- Authors
- Plump, A. S., Smith, J. D., Hayek, T., Aalto-Setälä, K., Walsh, A., Verstuyft, J. G., Rubin, E. M., & Breslow, J. L.
- Affiliations
- Rockefeller University (US)
- Lawrence Berkeley National Laboratory (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1992-10
- Volume
- 71
- Issue
- 2
- Pages
- 343-353
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2048890665
- Cited by
- 2,263
- References
- 45
- Citations by year
- 2026: 23 · 2025: 41 · 2024: 28 · 2023: 32 · 2022: 52 · 2021: 39 · 2020: 60 · 2019: 69 · 2018: 57 · 2017: 66 · 2016: 57 · 2015: 71 · 2014: 71 · 2013: 82 · 2012: 75
- FWCI
- 72.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl033714-17)
- National Heart, Lung, and Blood Institute (HL 33714, R01HL032435, HL32435)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipoproteins and Cardiovascular Health
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Keywords
- apolipoprotein E deficiency, hypercholesterolemia, atherosclerosis, ApoE-deficient mice, homologous recombination, embryonic stem cells, serum cholesterol, VLDL, intermediate density lipoprotein, high-fat diet, aortic atherosclerotic lesions, coronary arteries, pulmonary artery, animal models of atherosclerosis
- MeSH terms
- Animals, Aorta, Apolipoproteins E, Arteriosclerosis, Cholesterol, Coronary Vessels, Disease Models, Animal, Hypercholesterolemia, Lipoproteins, Lipoproteins, VLDL, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Pulmonary Artery, Stem Cells, Mice, Lipoproteins, IDL
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