Extraction and fractionation of microalgae-based protein products

IOP Conference Series: Earth and Environmental Science

Hermansah, H., Sandi, N., & Naspendra, Z. (2020) · IOP Conference Series Earth and Environmental Science · doi:10.1088/issn.1755-1315 · Cited by 12,775

Biofuels from Microalgae—A Review of Technologies for Production, Processing, and Extractions of Biofuels and Co-Products

Brennan, L., & Owende, P. (2010) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2009.10.009 · Cited by 4,894

Commercial Applications of Microalgae

Spolaore, P., Joannis-Cassan, C., Duran, E., & Isambert, A. (2006) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.101.87 · Cited by 4,285

Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor

Rodolfi, L., Chini Zittelli, G., Bassi, N., Padovani, G., Biondi, N., Bonini, G., & Tredici, M. R. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22033 · Cited by 2,879

Micro-algae as a source of protein

Becker, E. W. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2006.11.002 · Cited by 2,311

Recovery of microalgal biomass and metabolites: Process options and economics

Molina Grima, E., Belarbi, E.-H., Acién Fernández, F. G., Robles Medina, A., & Chisti, Y. (2003) · Biotechnology Advances · doi:10.1016/s0734-9750(02)00050-2 · Cited by 2,286

Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review

Chen, C.-Y., Yeh, K.-L., Aisyah, R., Lee, D.-J., & Chang, J.-S. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.159 · Cited by 1,860

Heterotrophic cultures of microalgae: Metabolism and potential products

Perez‐Garcia, O., Escalante, F. M. E., de‐Bashan, L. E., & Yoav Bashan, A. (2010) · Water Research · doi:10.1016/j.watres.2010.08.037 · Cited by 1,820

Algae as nutritional and functional food sources: revisiting our understanding.

Wells, M. L., Potin, P., Craigie, J. S., Raven, J. A., Merchant, S. S., Helliwell, K. E., Smith, A. G., Camire, M. E., & Brawley, S. H. (2017) · Journal of Applied Phycology · doi:10.1007/s10811-016-0974-5 · Cited by 1,562

Future Protein Supply and Demand: Strategies and Factors Influencing a Sustainable Equilibrium

Henchion, M., Hayes, M., Mullen, A., Fenelon, M., & Tiwari, B. (2017) · Foods · doi:10.3390/foods6070053 · Cited by 1,519

Biodiesel production from heterotrophic microalgal oil

Miao, X., & Wu, Q. (2006) · Bioresource Technology · doi:10.1016/j.biortech.2005.04.008 · Cited by 1,358

Comparison of several methods for effective lipid extraction from microalgae

Lee, J.-Y., Yoo, C., Jun, S.-Y., Ahn, C.-Y., & Oh, H.-M. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2009.03.058 · Cited by 1,279

Algal Proteins: Extraction, Application, and Challenges Concerning Production

Bleakley, S., & Hayes, M. (2017) · Foods · doi:10.3390/foods6050033 · Cited by 1,092

Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions

Liang, Y., Sarkany, N., & Cui, Y. (2009) · Biotechnology Letters · doi:10.1007/s10529-009-9975-7 · Cited by 1,059

Nutritional properties of microalgae for mariculture

Brown, M. R., Jeffrey, S. W., Volkman, J. K., & Dunstan, G. A. (1997) · Aquaculture · doi:10.1016/s0044-8486(96)01501-3 · Cited by 1,041

Micro-algae cultivation for biofuels: Cost, energy balance, environmental impacts and future prospects

Slade, R., & Bauen, A. (2013) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2012.12.019 · Cited by 978

Comparative energy life-cycle analyses of microalgal biomass production in open ponds and photobioreactors

Jorquera, O., Kiperstok, A., Sales, E. A., Embiruçu, M., & Ghirardi, M. L. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2009.09.038 · Cited by 863

Biochemistry Of Meat Hydration

Hamm, R. (1961) · Advances in food research · doi:10.1016/s0065-2628(08)60141-x · Cited by 837

Microalgal production — A close look at the economics

Norsker, N.-H., Barbosa, M. J., Vermuë, M. H., & Wijffels, R. H. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2010.08.005 · Cited by 819

Cell disruption for microalgae biorefineries

Günerken, E., D'Hondt, E., Eppink, M. H. M., Garcia‐Gonzalez, L., Elst, K., & Wijffels, R. H. (2015) · Biotechnology Advances · doi:10.1016/j.biotechadv.2015.01.008 · Cited by 801

Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation

Cheirsilp, B., & Torpee, S. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.01.125 · Cited by 782

Single Cell Protein—State-of-the-Art, Industrial Landscape and Patents 2001–2016

Ritala, A., Häkkinen, S. T., Toivari, M. H., & Wiebe, M. G. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.02009 · Cited by 754

Production cost of a real microalgae production plant and strategies to reduce it

Acién, F. G., Fernández, J. M., Magán, J. J., & Molina, E. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.02.005 · Cited by 735

Microalgae-based carbohydrates for biofuel production

Chen, C.-Y., Zhao, X.-Q., Yen, H.-W., Ho, S.-H., Cheng, C.-L., Lee, D.-J., Bai, F.-W., & Chang, J.-S. (2013) · Biochemical Engineering Journal · doi:10.1016/j.bej.2013.03.006 · Cited by 724

Spirulina Platensis Arthrospira

(1997) · doi:10.1201/9781482272970 · Cited by 704

Bioethanol production using carbohydrate-rich microalgae biomass as feedstock

Ho, S.-H., Huang, S.-W., Chen, C.-Y., Hasunuma, T., Kondo, A., & Chang, J.-S. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2012.10.015 · Cited by 683

Towards industrial products from microalgae

Ruiz, J., Olivieri, G., de Vree, J. H., Bosma, R., Willems, P., Reith, J. H., Eppink, M. H. M., Kleinegris, D. M. M., Wijffels, R. H., & Barbosa, M. J. (2016) · Energy & Environmental Science · doi:10.1039/c6ee01493c · Cited by 661

HANDBOOK OF MICROALGAL CULTURE: BIOTECHNOLOGY AND APPLIED PHYCOLOGY

Henry, E. C. (2004) · Journal of Phycology · doi:10.1111/j.1529-8817.2004.40502.x · Cited by 653

Microalgae in Human and Animal Nutrition

Becker, W. (2003) · Handbook of Microalgal Culture · doi:10.1002/9780470995280.ch18 · Cited by 613

Structural and Emulsifying Properties of Soy Protein Isolate Subjected to Acid and Alkaline pH-Shifting Processes

Jiang, J., Chen, J., & Xiong, Y. L. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf901585n · Cited by 597

Microalgal mass culture systems and methods: their limitation and potential

Lee, Y.-K. (2001) · Journal of Applied Phycology · doi:10.1023/a:1017560006941 · Cited by 528

Microalgae for the production of bulk chemicals and biofuels

Wijffels, R. H., Barbosa, M. J., & Eppink, M. H. M. (2010) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.215 · Cited by 522

Angiotensin Converting Enzyme Inhibitory Peptides Derived from Food Proteins: Biochemistry, Bioactivity and Production

Murray, B., & Fitzgerald, R. J. (2007) · Current Pharmaceutical Design · doi:10.2174/138161207780363068 · Cited by 486

Sustainable Protein Sources

(2016) · Elsevier eBooks · doi:10.1016/c2014-0-03542-3 · Cited by 461

Comparison of microalgal biomass profiles as novel functional ingredient for food products

Batista, A. P., Gouveia, L., Bandarra, N. M., Franco, J. M., & Raymundo, A. (2013) · Algal Research · doi:10.1016/j.algal.2013.01.004 · Cited by 450

Mixotrophic cultivation of Chlorella vulgaris using industrial dairy waste as organic carbon source

Abreu, A. P., Fernandes, B., Vicente, A. A., Teixeira, J., & Dragone, G. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.05.055 · Cited by 408

Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems

Sheih, I., Wu, T., & Fang, T. J. (2009) · Bioresource Technology · doi:10.1016/j.biortech.2009.02.014 · Cited by 405

Aqueous extraction of proteins from microalgae: Effect of different cell disruption methods

Safi, C., Ursu, A.-V., Laroche, C., Zebib, B., Merah, O., Pontalier, P.-Y., & Vaca‐García, C. (2014) · Algal Research · doi:10.1016/j.algal.2013.12.004 · Cited by 394

Extraction, fractionation and functional properties of proteins from the microalgae Chlorella vulgaris

Ursu, A.-V., Marcati, A., Sayd, T., Sante-Lhoutellier, V., Djelveh, G., & Michaud, P. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.01.071 · Cited by 367

Recent trends in the mass cultivation of algae in raceway ponds

Kumar, K., Mishra, S. K., Shrivastav, A., Park, M., & Yang, J.-W. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.06.033 · Cited by 342

Disruption of Chlorella vulgaris Cells for the Release of Biodiesel-Producing Lipids: A Comparison of Grinding, Ultrasonication, Bead Milling, Enzymatic Lysis, and Microwaves

Zheng, H., Yin, J., Gao, Z., Huang, H., Ji, X., & Dou, C. (2011) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-011-9207-1 · Cited by 340

Effect of pH on the functional properties of Arthrospira (Spirulina) platensis protein isolate

Benelhadj, S., Gharsallaoui, A., Degraeve, P., Attia, H., & Ghorbel, D. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.08.133 · Cited by 327

Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials

Heredia-Arroyo, T., Wei, W., Ruan, R. R., & Hu, B. (2011) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2011.02.036 · Cited by 326

Effects of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalga Chlorella vulgaris ESP-31

Yeh, K.-L., & Chang, J. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.11.103 · Cited by 317

Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp.

Schwenzfeier, A., Wierenga, P. A., & Gruppen, H. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.07.046 · Cited by 314

Selective extraction from microalgae Nannochloropsis sp. using different methods of cell disruption

Grimi, N., Dubois, A., Marchal, L., Jubeau, S., Lebovka, N., & Vorobiev, E. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.12.011 · Cited by 314

Autotrophic and heterotrophic microalgae and cyanobacteria cultivation for food and feed: life cycle assessment

Smetana, S. M., Sandmann, M., Rohn, S., Pleißner, D., & Heinz, V. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.08.113 · Cited by 309

Interfacial properties of whey protein and whey protein hydrolysates and their influence on O/W emulsion stability

Schröder, A., Berton‐Carabin, C., Venema, P., & Cornacchia, L. (2017) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2017.06.001 · Cited by 308

Nutritional Evaluation of Australian Microalgae as Potential Human Health Supplements

Kent, M., Welladsen, H. M., Mangott, A., & Li, Y. (2015) · PLoS ONE · doi:10.1371/journal.pone.0118985 · Cited by 304

Investigation of lifestyle choices of individuals following a vegan diet for health and ethical reasons

Radnitz, C. L., Beezhold, B., & DiMatteo, J. (2015) · Appetite · doi:10.1016/j.appet.2015.02.026 · Cited by 294

Marine-Based Nutraceuticals: An Innovative Trend in the Food and Supplement Industries

Suleria, H. A. R., Osborne, S. A., Masci, P. P., & Gobe, G. C. (2015) · Marine Drugs · doi:10.3390/md13106336 · Cited by 289

Influence of microalgae cell wall characteristics on protein extractability and determination of nitrogen-to-protein conversion factors

Safi, C., Charton, M., Pignolet, O., Silvestre, F., Vaca-Garcia, C., & Pontalier, P.-Y. (2013) · Journal of Applied Phycology · doi:10.1007/s10811-012-9886-1 · Cited by 283

Algal biofuels: Challenges and opportunities

Leite, G. B., Abdelaziz, A. E. M., & Hallenbeck, P. C. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.02.007 · Cited by 282

Multi-Product Microalgae Biorefineries: From Concept Towards Reality

Lam, G. P. ‘., Vermuë, M. H., Eppink, M. H. M., Wijffels, R. H., & van den Berg, C. (2017) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.10.011 · Cited by 265

Bioethanol production from the nutrient stress-induced microalga Chlorella vulgaris by enzymatic hydrolysis and immobilized yeast fermentation

Kim, K. H., Choi, I. S., Kim, H. M., Wi, S. G., & Bae, H. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.11.059 · Cited by 253

Anticoagulant activity of marine green and brown algae collected from Jeju Island in Korea

ATHUKORALA, Y., LEE, K., KIM, S., & JEON, Y. (2007) · Bioresource Technology · doi:10.1016/j.biortech.2006.07.034 · Cited by 253

The biotechnology of cultivating the halotolerant algaDunaliella

Ben‐Amotz, A., & Avron, M. (1990) · Trends in biotechnology · doi:10.1016/0167-7799(90)90152-n · Cited by 251

Enzyme assisted protein extraction from rapeseed, soybean, and microalgae meals

Sari, Y. W., Bruins, M. E., & Sanders, J. P. M. (2012) · Industrial Crops and Products · doi:10.1016/j.indcrop.2012.07.014 · Cited by 247

Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa

Waghmare, A. G., Salve, M. K., LeBlanc, J. G., & Arya, S. S. (2016) · Bioresources and Bioprocessing · doi:10.1186/s40643-016-0094-8 · Cited by 245

Heterotrophic/mixotrophic cultivation of oleaginous Chlorella vulgaris on industrial co-products

Mitra, D., van Leeuwen, J. (., & Lamsal, B. (2012) · Algal Research · doi:10.1016/j.algal.2012.03.002 · Cited by 239

Strategies to enhance the production of photosynthetic pigments and lipids in chlorophycae species

Benavente‐Valdés, J. R., Aguilar, C. N., Contreras‐Esquivel, J. C., Méndez‐Zavala, A., & Montañez, J. (2016) · Biotechnology Reports · doi:10.1016/j.btre.2016.04.001 · Cited by 235

Heterotrophic cultivation of microalgae using aquaculture wastewater: A biorefinery concept for biomass production and nutrient remediation

Guldhe, A., Ansari, F. A., Singh, P., & Bux, F. (2016) · Ecological Engineering · doi:10.1016/j.ecoleng.2016.11.013 · Cited by 234

Influence of processing parameters on disintegration of Chlorella cells in various types of homogenizers

Doucha, J., & Lívanský, K. (2008) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1660-6 · Cited by 231

Mild disintegration of the green microalgae Chlorella vulgaris using bead milling

Postma, P. R., Miron, T. L., Olivieri, G., Barbosa, M. J., Wijffels, R. H., & Eppink, M. H. M. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.09.033 · Cited by 223

Food proteins : properties and characterization

Nakai, S., & Modler, H. W. (1996) · Medical Entomology and Zoology · Cited by 217

Pulsed Electric Fields Technology for the Food Industry

Raso, J., & Heinz, V. (2006) · Food engineering series · doi:10.1007/978-0-387-31122-7 · Cited by 217

Dewatering and Drying Methods for Microalgae

Chen, C.-L., Chang, J., & Lee, D. (2015) · Drying Technology · doi:10.1080/07373937.2014.997881 · Cited by 217

Identification and Inhibitory Properties of Multifunctional Peptides from Pea Protein Hydrolysate

Li, H., & Aluko, R. E. (2010) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf102538g · Cited by 213

Purification of novel anti-inflammatory peptides from enzymatic hydrolysate of the edible microalgal Spirulina maxima

Vo, T.-S., Ryu, B., & Kim, S.-K. (2013) · Journal of Functional Foods · doi:10.1016/j.jff.2013.05.001 · Cited by 209

Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field–temperature treatment

Postma, P. R., Pataro, G., Capitoli, M. M., Barbosa, M. J., Wijffels, R. H., Eppink, M. H. M., Olivieri, G., & Ferrari, G. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.12.012 · Cited by 202

DISTRIBUTION OF INTRACELLULAR NITROGEN IN MARINE MICROALGAE: BASIS FOR THE CALCULATION OF SPECIFIC NITROGEN‐TO‐PROTEIN CONVERSION FACTORS

Lourenço, S. O., Barbarino, E., Marquez, Ú. M. L., & Aidar, E. (1998) · Journal of Phycology · doi:10.1046/j.1529-8817.1998.340798.x · Cited by 191

Effects of nitrogen sources on cell growth and biochemical composition of marine chlorophyte Tetraselmis sp. for lipid production

Kim, G., Mujtaba, G., & Lee, K. (2016) · ALGAE · doi:10.4490/algae.2016.31.8.18 · Cited by 178

Enzymatic cell disruption of the microalgae Chlamydomonas reinhardtii for lipid and protein extraction

Sierra, L. S., Dixon, C. K., & Wilken, L. R. (2017) · Algal Research · doi:10.1016/j.algal.2017.04.004 · Cited by 176

Technology of Production of Edible Flours and Protein Products from Soybeans

Berk, Z. (1992) · Medical Entomology and Zoology · Cited by 175

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Chua, E. T., & Schenk, P. M. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.05.124 · Cited by 174

Effects of CO2 concentrations on the freshwater microalgae, Chlamydomonas reinhardtii, Chlorella pyrenoidosa and Scenedesmus obliquus (Chlorophyta)

Yang, Y., & Gao, K. (2003) · Journal of Applied Phycology · doi:10.1023/a:1026021021774 · Cited by 171

Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects

Gong, Y., Hu, H., Gao, Y., Xu, X., & Gao, H. C. (2011) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-011-1032-6 · Cited by 168

Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass

Gerde, J. A., Wang, T., Yao, L., Jung, S., Johnson, L. A., & Lamsal, B. P. (2013) · Algal Research · doi:10.1016/j.algal.2013.02.001 · Cited by 166

Functional properties and health benefits of bioactive peptides derived from Spirulina : A review

Ovando, C. A., de Carvalho, J. C., Pereira, G. V. D. M., Jacques, P., Thomaz‐Soccol, V., & Soccol, C. R. (2016) · Food Reviews International · doi:10.1080/87559129.2016.1210632 · Cited by 164

Angiotensin I-converting enzyme inhibitor derived from soy protein hydrolysate and produced by using membrane reactor

Chiang, W., Tsou, M.-J., Tsai, Z.-Y., & Tsai, T.-C. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2005.06.038 · Cited by 158

Green microalgae biomolecule separations and recovery

Dixon, C. K., & Wilken, L. R. (2018) · Bioresources and Bioprocessing · doi:10.1186/s40643-018-0199-3 · Cited by 155

Understanding the effect of cell disruption methods on the diffusion of Chlorella vulgaris proteins and pigments in the aqueous phase

Safi, C., Frances, C., Ursu, A. V., Laroche, C., Pouzet, C., Vaca-Garcia, C., & Pontalier, P.-Y. (2015) · Algal Research · doi:10.1016/j.algal.2015.01.002 · Cited by 151

Applications of Pulsed Electric Fields Technology for the Food Industry

Toepfl, S., Heinz, V., & Knorr, D. (2006) · Food engineering series · doi:10.1007/978-0-387-31122-7_7 · Cited by 139

An investigation of ultrasound effect on microalgal cell integrity and lipid extraction efficiency

Keris-Sen, U. D., Sen, U., Soydemir, G., & Gurol, M. D. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.11.018 · Cited by 139

Microalgae as a potential source of single-cell proteins. A review

Barka, A., & Blecker, C. (2016) · BASE · doi:10.25518/1780-4507.13132 · Cited by 138

Pea proteins: A review of chemistry, technology of production, and utilization

Owusu-Ansah, Y. J., & McCURDY, S. M. (1991) · Food Reviews International · doi:10.1080/87559129109540903 · Cited by 136

Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana

Safi, C., Rodriguez, L. C., Mulder, W., Engelen-Smit, N., Spekking, W. T. J., van den Broek, L. A. M., Olivieri, G., & Sijtsma, L. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.05.012 · Cited by 135

Production of high-density Chlorella culture grown in fermenters

Doucha, J., & Lívanský, K. (2011) · Journal of Applied Phycology · doi:10.1007/s10811-010-9643-2 · Cited by 133

Comparative studies onChlorella cell walls: Induction of protoplast formation

Yamada, T., & Sakaguchi, K. (1982) · Archives of Microbiology · doi:10.1007/bf00690809 · Cited by 129

Protein and carbohydrate extraction from S. platensis biomass by ultrasound and mechanical agitation

Menegotto, A. L. L., Bispo, L. D. O., Colla, L. M., Costa, J. A. V., Canan, C., & Colla, E. (2016) · Food Research International · doi:10.1016/j.foodres.2016.11.036 · Cited by 126

Utilisation of Chlorellavulgaris cell biomass for the production of enzymatic protein hydrolysates

Morris, H. J., Almarales, Á., Carrillo-Farnés, O., & Bermúdez, R. C. (2008) · Bioresource Technology · doi:10.1016/j.biortech.2008.01.080 · Cited by 124

Influence of the Cell Wall on Intracellular Delivery to Algal Cells by Electroporation and Sonication

Azencott, H. R., Peter, G. F., & Prausnitz, M. R. (2007) · Ultrasound in Medicine & Biology · doi:10.1016/j.ultrasmedbio.2007.05.008 · Cited by 121

Biorefinery of microalgal soluble proteins by sequential processing and membrane filtration

Safi, C., Olivieri, G., Campos, R. P., Engelen-Smit, N., Mulder, W., van den Broek, L. A. M., & Sijtsma, L. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.11.068 · Cited by 120

Process for selective extraction of pigments and functional proteins from Chlorella vulgaris

Kulkarni, S., & Nikolov, Z. L. (2018) · Algal Research · doi:10.1016/j.algal.2018.08.024 · Cited by 117

Biomass Volume Estimation and Valorization for Energy

Tumuluru, J. S. (2017) · InTech eBooks · doi:10.5772/62678 · Cited by 110

Effect of Cell Size and Shape on Single-Cell Electroporation

Agarwal, A., Zudans, I., Weber, E. A., Olofsson, J., Orwar, O., & Weber, S. G. (2007) · Analytical Chemistry · doi:10.1021/ac062049e · Cited by 109

Lipid extracted algae as a source for protein and reduced sugar: A step closer to the biorefinery

Ansari, F. A., Shriwastav, A., Gupta, S. K., Rawat, I., Guldhe, A., & Bux, F. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.12.047 · Cited by 108

Sequential heterotrophic/autotrophic cultivation – An efficient method of producing Chlorella biomass for health food and animal feed

Ogbonna, J. C., Masui, H., & Tanaka, H. (1997) · Journal of Applied Phycology · doi:10.1023/a:1007981930676 · Cited by 104

Optimization of protein electroextraction from microalgae by a flow process

Coustets, M., Joubert-Durigneux, V., Hérault, J., Schoefs, B., Blanckaert, V. D., Garnier, J.-P., & Teissié, J. (2014) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2014.08.022 · Cited by 100

Evaluating the industrial potential of biodiesel from a microalgae heterotrophic culture: Scale-up and economics

Tabernero, A., del Valle, E. M. M., & Galán, M. Á. (2011) · Biochemical Engineering Journal · doi:10.1016/j.bej.2011.11.006 · Cited by 97

Effect of drying and extraction conditions on the recovery of bioactive compounds from Chlorella vulgaris

Stramarkou, M., Papadaki, S., Kyriakopoulou, K., & Krokida, M. K. (2017) · Journal of Applied Phycology · doi:10.1007/s10811-017-1181-8 · Cited by 96

Investigation of in vitro digestibility of dietary microalga Chlorella vulgaris and cyanobacterium Spirulina platensis as a nutritional supplement

Köse, A., Ozen, M. O., Elibol, M., & Öncel, S. Ş. (2017) · 3 Biotech · doi:10.1007/s13205-017-0832-4 · Cited by 95

Effects of solvent and n-3 rich fish oil on physicochemical properties of electrospun zein fibres

Moomand, K., & Lim, L. (2014) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2014.12.014 · Cited by 94

Effects of different photoperiod and trophic conditions on biomass, protein and lipid production by the marine alga Nannochloropsis gaditana at optimal concentration of desalination concentrate

Matos, Â. P., Cavanholi, M. G., Moecke, E. H. S., & Sant'Anna, E. S. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2016.11.004 · Cited by 91

Comparison of Protein Extracts from Various Unicellular Green Sources

Teuling, E., Wierenga, P. A., Schrama, J. W., & Gruppen, H. (2017) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.7b01788 · Cited by 91

Mixotrophic cultivation of Chlorella for local protein production using agro-food by-products

Salati, S., D’Imporzano, G., Menin, B., Veronesi, D., Scaglia, B., Abbruscato, P., Mariani, P., & Adani, F. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.01.030 · Cited by 89

Production of whey protein hydrolysates with reduced allergenicity in a stable membrane reactor

Guadix, A., Camacho, F., & Guadix, E. M. (2005) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2004.12.022 · Cited by 87

Enzymatic pre‐treatment of microalgae cells for enhanced extraction of proteins

Al‐Zuhair, S., Ashraf, S. M., Hisaindee, S., Darmaki, N. A., Battah, S., Svistunenko, D. A., Reeder, B. J., Stanway, G., & Chaudhary, A. K. (2016) · Engineering in Life Sciences · doi:10.1002/elsc.201600127 · Cited by 87

Exploration of Microalgae Biorefinery by Optimizing Sequential Extraction of Major Metabolites from Scenedesmus obliquus

Ansari, F. A., Shriwastav, A., Gupta, S. K., Rawat, I., & Bux, F. (2017) · Industrial & Engineering Chemistry Research · doi:10.1021/acs.iecr.6b04814 · Cited by 83

The development of electro-membrane filtration for the isolation of bioactive peptides: the effect of membrane selection and operating parameters on the transport rate

Bargeman, G., Koops, G. H., Houwing, J., Breebaart, I., van der Horst, H. C., & Weßling, M. (2002) · Desalination · doi:10.1016/s0011-9164(02)00824-x · Cited by 82

Antioxidant Peptides from Protein Hydrolysate of Microalgae Navicula incerta and their Protective Effects in Hepg2/CYP2E1 Cells Induced by Ethanol

Kang, K., Qian, Z., Ryu, B., Karadeniz, F., Kim, D., & Kim, S. (2012) · Phytotherapy Research · doi:10.1002/ptr.4603 · Cited by 79

Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions

Chen, F., Chen, H., & Gong, X. (1997) · Bioresource Technology · doi:10.1016/s0960-8524(97)00115-6 · Cited by 74

Specific electropermeabilization of leucocytes in a blood sample and application to large volumes of cells

Sixou, S., & Teissié, J. (1990) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(90)90149-i · Cited by 71

Production of Intracellular Phytochemicals in Chlorella under Heterotrophic Conditions

Sansawa, H., & Endô, H. (2004) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(05)00309-9 · Cited by 69

The CIDES process: Fractionation of concentrated microalgal paste for co-production of biofuel, nutraceuticals, and high-grade protein feed

Halim, R., Webley, P. A., & Martin, G. J. O. (2015) · Algal Research · doi:10.1016/j.algal.2015.09.018 · Cited by 67

Residual biomasses and protein hydrolysates of three green microalgae species exhibit antioxidant and anti-aging activity

Norzagaray-Valenzuela, C. D., Valdez-Ortíz, Á., Shelton, L. M., Jiménez‐Edeza, M., Rivera‐López, J., Valdez-Flores, M. A., & Germán-Báez, L. J. (2016) · Journal of Applied Phycology · doi:10.1007/s10811-016-0938-9 · Cited by 61

Process for Preparing Value-Added Products from Microalgae Using Textile Effluent through a Biorefinery Approach

Bhattacharya, S., Pramanik, S. K., Gehlot, P. S., Patel, H., Gajaria, T. K., Mishra, S., & Kumar, A. (2017) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.7b01961 · Cited by 57

Properties of microalgal enzymatic protein hydrolysates: Biochemical composition, protein distribution and FTIR characteristics

Köse, A., & Öncel, S. Ş. (2015) · Biotechnology Reports · doi:10.1016/j.btre.2015.02.005 · Cited by 56

Cyanobacteria and eukaryotic microalgae as potential sources of antibiotics

Senhorinho, G. N. A., Ross, G. M., & Scott, J. A. (2015) · Phycologia · doi:10.2216/14-092.1 · Cited by 55

Development of Bioactive Peptides from Fish Proteins and Their Health Promoting Ability

Senevirathne, M., & Kim, S. (2012) · Advances in food and nutrition research · doi:10.1016/b978-0-12-416003-3.00015-9 · Cited by 55

Electro-membrane fractionation of antioxidant peptides from protein hydrolysates of rainbow trout (Oncorhynchus mykiss) byproducts

Suwal, S., Ketnawa, S., Liceaga, A. M., & Huang, J. (2017) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2017.08.016 · Cited by 55

Protective Effect of Enzymatic Extracts from Microalgae Against DNA Damage Induced by H2O2

Karawita, R., Senevirathne, M., Athukorala, Y., Affan, A., Lee, Y. J., Kim, S., Lee, J.-B., & Jeon, Y. (2007) · Marine Biotechnology · doi:10.1007/s10126-007-9007-3 · Cited by 53

High Density Outdoor Microalgal Culture

Doucha, J., & Lívanský, K. (2014) · Algal Biorefineries · doi:10.1007/978-94-007-7494-0_6 · Cited by 50

Biosystems Engineering: Biofactories for Food Production in the Century XXI

Guevara-González, R. G., & Torres‐Pacheco, I. (2014) · Springer eBooks · doi:10.1007/978-3-319-03880-3 · Cited by 50

Tetrameric peptide purified from hydrolysates of biodiesel byproducts of Nannochloropsis oculata induces osteoblastic differentiation through MAPK and Smad pathway on MG-63 and D1 cells

Nguyen, M. H. T., Qian, Z., Nguyen, V.-T., Choi, I., Heo, S., Oh, C. H., Kang, D.-H., Kim, G., & Jung, W. (2013) · Process Biochemistry · doi:10.1016/j.procbio.2013.06.031 · Cited by 49

The effects of nitrogen and phosphorus deficiencies and nitrite addition on the lipid content of Chlorella vulgaris (Chlorophyceae)

Mutlu, Y., Uslu, L., Ko, K., & Durmaz, Y. (2011) · AFRICAN JOURNAL OF BIOTECHNOLOGY · Cited by 49

Drying of alga as a source of bioenergy feedstock and food supplement – A review

Bennamoun, L., Afzal, M. T., & Léonard, A. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.04.196 · Cited by 42

Role of phosphoenolpyruvate carboxylase in anaplerosis in the green microalga Dunaliella salina cultured under different nitrogen regimes

Norici, A., Dalsass, A., & Giordano, M. (2002) · Physiologia Plantarum · doi:10.1034/j.1399-3054.2002.1160207.x · Cited by 42

Protein Fractions with Techno-Functional and Antioxidant Properties from Nannochloropsis gaditana Microalgal Biomass

Medina, C., Rubilar, M., Shene, C., Torres, S., & Verdugo, M. (2015) · Journal of Biobased Materials and Bioenergy · doi:10.1166/jbmb.2015.1534 · Cited by 42

Protein targeting to the chloroplasts of photosynthetic eukaryotes: getting there is half the fun

Nassoury, N., & Morse, D. (2004) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2004.09.017 · Cited by 40

MICROALGAL BIOTRANSFORMATION OF STEROIDS1

Faramarzi, M. A., Adrangi, S., & Yazdi, M. T. (2008) · Journal of Phycology · doi:10.1111/j.1529-8817.2007.00446.x · Cited by 36

Microstructure of Spray-Dried and Freeze-Dried Microalgal Powders

Lin, L. P. (1985) · Digital Commons - USU (Utah State University) · Cited by 36

Heterotrophic Microalgae

Klamczynska, B., & Mooney, W. D. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-802778-3.00020-2 · Cited by 35

Enzymatic hydrolysis of cell proteins in green algae Chlorella and Scenedesmus after extraction with organic solvents

Tchorbanov, B., & Божкова, М. (1988) · Enzyme and Microbial Technology · doi:10.1016/0141-0229(88)90072-5 · Cited by 26

Fractionation of dairy based functional peptides using ion-exchange membrane adsorption chromatography and cross-flow electro membrane filtration

Leeb, E., Holder, A., Letzel, T., Cheison, S. C., Kulozik, U., & Hinrichs, J. (2014) · International Dairy Journal · doi:10.1016/j.idairyj.2013.12.006 · Cited by 26

Techno-economical evaluation of protein extraction for microalgae biorefinery

Sari, Y. W., Sanders, J. P. M., & Bruins, M. E. (2016) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/31/1/012034 · Cited by 24

Disintegration of Nannochloropsis sp. cells in an improved turbine bead mill

Pan, Z., Huang, Y. L., Wang, Y., & Wu, Z. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.08.146 · Cited by 19

Microalgae and Cyanobacteria Production for Feed and Food Supplements

Vanthoor-Koopmans, M., Cordoba-Matson, M. V., Arredondo-Vega, B. O., Lozano-Ramírez, C., García‐Trejo, J. F., & Rodríguez-Palacio, M. C. (2014) · Springer eBooks · doi:10.1007/978-3-319-03880-3_8 · Cited by 17

Microalgal Biomass: A Biorefinery Approach

Fernández-Linares, L. C., Falfán, K. Á. G., & Ramírez-López, C. (2017) · InTech eBooks · doi:10.5772/65827 · Cited by 17

AlgaeEconomics: bio-economic production models of micro-algae and downstream processing to produce bio energy carriers

Spruijt, J., Schipperus, R., Kootstra, A. M. J., & de Visser, C. L. M. (2015) · Socio-Environmental Systems Modeling · Cited by 15

Economic Feasibility Analysis of the Industrial Production of Fish Protein Hydrolysates using Conceptual Process Simulation Software

Christopher, S. H. (2014) · Journal of Bioprocessing & Biotechniques · doi:10.4172/2155-9821.1000191 · Cited by 13

PROTEIN ISOLATES FROM CHLORELLA ALGAE, TORULA YEASTS, AND HYDROCARBON-ASSIMILATING MICROORGANISMS

Mitsuda, H. (1973) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.19.1 · Cited by 8

Utilization of non-conventional systems for conversion of biomass to food components

Karel, M., & Nakhost, Z. (1989) · NASA STI Repository (National Aeronautics and Space Administration) · Cited by 7

Biorefinery of microalgae

Wijffels, R. H., & Barbosa, M. J. (2011) · Socio-Environmental Systems Modeling · Cited by 2

Microalgae wastewater treatment: Biological and technological approaches

Wollmann, F., Dietze, S., Ackermann, J., Bley, T., Walther, T., Steingroewer, J., & Krujatz, F. (2019) · Engineering in Life Sciences · doi:10.1002/elsc.201900071 · Cited by 369

Production of microalgae with high lipid content and their potential as sources of nutraceuticals

Udayan, A., Pandey, A. K., Sirohi, R., Sreekumar, N., Sang, B.-I., Sim, S. J., Kim, S. H., & Pandey, A. (2023) · Phytochemistry Reviews · doi:10.1007/s11101-021-09784-y · Cited by 302

Algal proteins: Production strategies and nutritional and functional properties

Geada, P., Moreira, C., Silva, M., Nunes, R., Madureira, L., Rocha, C. M. R., Pereira, R. N., Vicente, A. A., & Teixeira, J. A. (2021) · Bioresource Technology · doi:10.1016/j.biortech.2021.125125 · Cited by 301

Bioactive food compounds from microalgae: an innovative framework on industrial biorefineries

Lopes, E. J., Maroneze, M. M., Deprá, M. C., Sartori, R. B., Dias, R. R., & Zepka, L. Q. (2018) · Current Opinion in Food Science · doi:10.1016/j.cofs.2018.12.003 · Cited by 255

Phycocyanin from Spirulina: A Review of Extraction Methods and Stability

Pez Jaeschke, D., Rocha Teixeira, I., Damasceno Ferreira Marczak, L., & Domeneghini Mercali, G. (2021) · Food Research International · doi:10.1016/j.foodres.2021.110314 · Cited by 254

Process development of enzymatically-generated algal protein hydrolysates for specialty food applications

Sierra, L. S., Wilken, L. R., Mallawarachchi, S., & Nikolov, Z. L. (2021) · Algal Research · doi:10.1016/j.algal.2021.102248 · Cited by 38

Microalgal proteins: Extraction, interfacial properties, bioactivities, and future perspectives – A review

Wang, J., Li, J., Yu, W., Wang, G., Cifuentes, A., Ibáñez, E., & Lu, W. (2025) · Food Chemistry · doi:10.1016/j.foodchem.2025.144680 · Cited by 15

Microalgal-Based Protein By-Products: Extraction, Purification, and Applications

Chiong, T., Acquah, C., Lau, S. Y., Khor, E. H., & Danquah, M. K. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-802391-4.00012-4 · Cited by 17

Production of Microalgae-based Protein Products from Economics and Bioprocessing Perspectives

Sierra, L. S. (2020) · OakTrust (Texas A&M University Libraries)

Enhancing microalgal proteins for nutraceutical and functional food applications

Kaur, M., Bhatia, S., Bagchi, D., Tak, Y., Kaur, G., Kaur, C., Kaur, A., & Sharma, N. (2025) · Future Foods · doi:10.1016/j.fufo.2025.100564 · Cited by 19