Cited by 52Circadian rhythm and melatoninEstrogen and related hormone effectsHypothalamic control of reproductive hormones

Effect of estrogen on the expression of Cry1 and Cry2 mRNAs in the suprachiasmatic nucleus of female rats

Nakamura, Takahiro J, Shinohara, Kazuyuki, Funabashi, Toshiya, Kimura, Fukuko·2001·Neuroscience Research

Abstract

To determine whether estrogen has an effect on clock genes in the suprachiasmatic nucleus (SCN), we examined the effect of estrogen on the expression of Cry1 and Cry2 mRNAs in the SCN of female rats. Ovariectomized rats were injected with 20 microg 17beta-estradiol at zeitgeber time (ZT) 6 and ZT 18 and killed 24 h after the treatment. Northern blot revealed that the expression of Cry2 mRNA was significantly decreased in the SCN of estrogen-treated rats at both time points [correction]. But estrogen did not affect Cry1 mRNA levels in the SCN at any ZT. These results suggested that Cry1 and Cry2 mRNAs in the SCN were differently regulated by estrogen.

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Crossref · 10.1016/s0168-0102(01)00285-1

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Authors
Nakamura, T. J., Shinohara, K., Funabashi, T., & Kimura, F.
Affiliations
  • Yokohama City University (JP)
  • Dokkyo University (JP)
Corresponding author
Shinohara, Kazuyuki
Venue
Neuroscience Research
Publisher
Elsevier BV
Published
2001-11
Volume
41
Issue
3
Pages
251-255
ISSN
0168-0102 (ISSN-L)
OpenAlex
W2075158731
Cited by
52
References
17
Citations by year
2026: 1 · 2025: 2 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 4 · 2014: 2 · 2013: 2 · 2012: 2
FWCI
0.47
Type
journal article
Language
en
Open access
closed
Locations
  • Neuroscience Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Keywords
estrogen, estradiol, suprachiasmatic nucleus, CRY1, CRY2, clock genes, mRNA expression, circadian rhythm, ovariectomy, female rats
MeSH terms
Animals, Biological Clocks, Circadian Rhythm, Estradiol, Eye Proteins, Female, Flavoproteins, Ovariectomy, RNA, Messenger, Suprachiasmatic Nucleus, Time Factors, Rats, Wistar, Photoreceptor Cells, Invertebrate, Drosophila Proteins, Receptors, G-Protein-Coupled, Rats, Cryptochromes

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