Effect of estrogen on the expression of Cry1 and Cry2 mRNAs in the suprachiasmatic nucleus of female rats
Abstract
To determine whether estrogen has an effect on clock genes in the suprachiasmatic nucleus (SCN), we examined the effect of estrogen on the expression of Cry1 and Cry2 mRNAs in the SCN of female rats. Ovariectomized rats were injected with 20 microg 17beta-estradiol at zeitgeber time (ZT) 6 and ZT 18 and killed 24 h after the treatment. Northern blot revealed that the expression of Cry2 mRNA was significantly decreased in the SCN of estrogen-treated rats at both time points [correction]. But estrogen did not affect Cry1 mRNA levels in the SCN at any ZT. These results suggested that Cry1 and Cry2 mRNAs in the SCN were differently regulated by estrogen.
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The phasing and amplitude of clock gene rhythms (Per2, Cry2) are sensitive to estrogen in females.
“For example, the phasing and amplitude of clock gene rhythms (Per2, Cry2) are sensitive to estrogen in females [133, 134].”
From The neuroendocrine control of the circadian system: Adolescent chronotype (2012) · cites this paper as [134]
The phasing and amplitude of clock gene rhythms (Per2, Cry2) are sensitive to estrogen in females.
“For example, the phasing and amplitude of clock gene rhythms (Per2, Cry2) are sensitive to estrogen in females [133, 134].”
From The neuroendocrine control of the circadian system: Adolescent chronotype (2012) · cites this paper as [133]
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Clock genes and female reproduction
Chen, C. (2009) · ERA
- Authors
- Nakamura, T. J., Shinohara, K., Funabashi, T., & Kimura, F.
- Affiliations
- Yokohama City University (JP)
- Dokkyo University (JP)
- Corresponding author
- Shinohara, Kazuyuki
- Venue
- Neuroscience Research
- Publisher
- Elsevier BV
- Published
- 2001-11
- Volume
- 41
- Issue
- 3
- Pages
- 251-255
- ISSN
- 0168-0102 (ISSN-L)
- OpenAlex
- W2075158731
- Cited by
- 52
- References
- 17
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 4 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- estrogen, estradiol, suprachiasmatic nucleus, CRY1, CRY2, clock genes, mRNA expression, circadian rhythm, ovariectomy, female rats
- MeSH terms
- Animals, Biological Clocks, Circadian Rhythm, Estradiol, Eye Proteins, Female, Flavoproteins, Ovariectomy, RNA, Messenger, Suprachiasmatic Nucleus, Time Factors, Rats, Wistar, Photoreceptor Cells, Invertebrate, Drosophila Proteins, Receptors, G-Protein-Coupled, Rats, Cryptochromes
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