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Ligands for Clathrin-Mediated Endocytosis Are Differentially Sorted into Distinct Populations of Early Endosomes

Lakadamyali, Melike, Rust, Michael J., Zhuang, Xiaowei·2006·Cell
DOI 10.1016/j.cell.2005.12.038
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Abstract

Cells rely on the correct sorting of endocytic ligands and receptors for proper function. Early endosomes have been considered as the initial sorting station where cargos for degradation separate from those for recycling. Using live-cell imaging to monitor individual endosomes and ligand particles in real time, we have discovered a sorting mechanism that takes place prior to early endosome entry. We show that early endosomes are in fact comprised of two distinct populations: a dynamic population that is highly mobile on microtubules and matures rapidly toward late endosomes and a static population that matures much more slowly. Several cargos destined for degradation are preferentially targeted to the dynamic endosomes, whereas the recycling ligand transferrin is nonselectively delivered to all early endosomes and effectively enriched in the larger, static population. This pre-early endosome sorting process begins at clathrin-coated vesicles, depends on microtubule-dependent motility, and appears to involve endocytic adaptors.

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  • There are conflicting reports as to whether LDLR and TfnR are internalized via the same or distinct CCPs.

    “there are conflicting reports as to whether LDLR and TfnR are internalized via the same or distinct CCPs (Keyel et al., 2006; Lakadamyali et al., 2006).”

    From Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis (2010) · cites this paper as Lakadamyali et al., 2006

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Metadata

Authors
Lakadamyali, M., Rust, M. J., & Zhuang, X.
Venue
Cell
Publisher
Elsevier BV
Published
2006-03
Volume
124
Issue
5
Pages
997-1009
ISSN
0092-8674
DOI
10.1016/j.cell.2005.12.038
Cited by
508
References
1
Language
en
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