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The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a

Liu, Liang, Li, Xueyan, Ma, Jun, Li, Zongqiang, You, Lilan, Wang, Jiuyu, Wang, Min, Zhang, Xinzheng, Wang, Yanli·2017·Cell
DOI 10.1016/j.cell.2017.06.050
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Abstract

Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. To understand how Cas13a is activated to cleave RNA, we have determined the crystal structure of Leptotrichia buccalis (Lbu) Cas13a bound to crRNA and its target RNA, as well as the cryo-EM structure of the LbuCas13a-crRNA complex. The crRNA-target RNA duplex binds in a positively charged central channel of the nuclease (NUC) lobe, and Cas13a protein and crRNA undergo a significant conformational change upon target RNA binding. The guide-target RNA duplex formation triggers HEPN1 domain to move toward HEPN2 domain, activating the HEPN catalytic site of Cas13a protein, which subsequently cleaves both single-stranded target and collateral RNAs in a non-specific manner. These findings reveal how Cas13a of type VI CRISPR-Cas systems defend against RNA phages and set the stage for its development as a tool for RNA manipulation.

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Metadata

Authors
Liu, L., Li, X., Ma, J., Li, Z., You, L., Wang, J., Wang, M., Zhang, X., & Wang, Y.
Venue
Cell
Publisher
Elsevier BV
Published
2017-08
Volume
170
Issue
4
Pages
714-726.e10
ISSN
0092-8674
DOI
10.1016/j.cell.2017.06.050
Cited by
538
References
3
Language
en
Licence
Funders
  • Salt Science Research Foundation
  • National Natural Science Foundation of China (31400640, 31570874, 31571335, 91440201)
  • Chinese Academy of Sciences (XDB08010203)
  • Ministry of Science and Technology of the People's Republic of China (2014CB910102)

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