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Cryo-EM Structure of the Human FLCN-FNIP2-Rag-Ragulator Complex
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“while the only other structures known are Longin and DENN domain-containing dimers of C9orf72/SMCR8 and Folliculin/FNIP2 that act as RAB GAP complexes.83,84,85,86,87”
From Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome (2023) · cites this paper as [85]
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The Rag GTPases Bind Raptor and Mediate Amino Acid Signaling to mTORC1
Sancak, Y., Peterson, T. R., Shaul, Y. D., Lindquist, R. A., Thoreen, C. C., Bar-Peled, L., & Sabatini, D. M. (2008) · Science · doi:10.1126/science.1157535 · Cited by 2,773
Self-citationSancak, Y., Bar-Peled, L., Zoncu, R., Markhard, A. L., Nada, S., & Sabatini, D. M. (2010) · Cell · doi:10.1016/j.cell.2010.02.024 · Cited by 2,464 · cited 1×
“Of these regulators, Ragulator is a pentameric protein complex that serves as a lysosomal membrane anchor for the Rag GTPases and also has non-canonical guanine exchange factor (GEF) activity toward RagC (Bar-Peled et al., 2012; Sancak et al., 2010; Shen and Sabatini, 2018).”
BackgroundSelf-citationRheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling
Inoki, K., Li, Y., Xu, T., & Guan, K.-L. (2003) · Genes & Development · doi:10.1101/gad.1110003 · Cited by 1,911
The Ras-RasGAP Complex: Structural Basis for GTPase Activation and Its Loss in Oncogenic Ras Mutants
Scheffzek, K., Ahmadian, M. R., Kabsch, W., Wiesmüller, L., Lautwein, A., Schmitz, F., & Wittinghofer, A. (1997) · Science · doi:10.1126/science.277.5324.333 · Cited by 1,543
Regulation of TORC1 by Rag GTPases in nutrient response
Kim, E., Goraksha-Hicks, P., Li, L., Neufeld, T. P., & Guan, K.-L. (2008) · Nature Cell Biology · doi:10.1038/ncb1753 · Cited by 1,349
Bar-Peled, L., Chantranupong, L., Cherniack, A. D., Chen, W. W., Ottina, K. A., Grabiner, B. C., Spear, E. D., Carter, S. L., Meyerson, M., & Sabatini, D. M. (2013) · Science · doi:10.1126/science.1232044 · Cited by 1,080 · cited 2×
“To determine the specificity of our assay, we carried out similar assays using RagA(Q66L) or RagC(Q120L) mutants, which have been shown to suppress the stimulatory effect of GATOR1 or FLCN-FNIP2, respectively (Bar-Peled et al., 2013; Tsun et al., 2013).”
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Metadata
- Authors
- Shen, K., Rogala, K. B., Chou, H.-T., Huang, R. K., Yu, Z., & Sabatini, D. M.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2019-11
- Volume
- 179
- Issue
- 6
- Pages
- 1319-1329.e8
- ISSN
- 0092-8674
- Cited by
- 134
- References
- 20
- Language
- en
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 392 d)
- Funders
- U.S. Department of Defense (W81XWH-15-1-0230)
- Howard Hughes Medical Institute
- American Cancer Society
- Lustgarten Foundation
- Life Sciences Research Foundation
- National Institutes of Health (R01 CA103866, R01 CA129105, R37 AI047389)
- Tuberous Sclerosis Association
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