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CRISPR-Cas systems: Overview, innovations and applications in human disease research and gene therapy
Open accessAbstract
Genome editing is the modification of genomic DNA at a specific target site in a wide variety of cell types and organisms, including insertion, deletion and replacement of DNA, resulting in inactivation of target genes, acquisition of novel genetic traits and correction of pathogenic gene mutations. Due to the advantages of simple design, low cost, high efficiency, good repeatability and short-cycle, CRISPR-Cas systems have become the most widely used genome editing technology in molecular biology laboratories all around the world. In this review, an overview of the CRISPR-Cas systems will be introduced, including the innovations, the applications in human disease research and gene therapy, as well as the challenges and opportunities that will be faced in the practical application of CRISPR-Cas systems.
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Citations
Cites
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The application of the CRISPR-Cas system in antibiotic resistance
Tao, S., Chen, H., Li, N., & Liang, W. (2022) · Infection and Drug Resistance · doi:10.2147/idr.s370869 · Cited by 147
The vibriophage-encoded inhibitor OrbA abrogates BREX-mediated defense through the ATPase BrxC
Oshiro, R. T., Dunham, D. T., & Seed, K. D. (2024) · Journal of Bacteriology · doi:10.1128/jb.00206-24 · Cited by 15
Sliti, A., Kim, R.-H., Lee, D., & Shin, J.-H. (2025) · Microbial Pathogenesis · doi:10.1016/j.micpath.2025.107405 · Cited by 5
Uti, D. E., Egbung, J. E., Anthony, B. O., Omari, O. R., Omang, W. A., Aggad, W. S., Almohaimeed, H. M., Bainsal, N., Shukla, S. K., Ealla, K. K. R., et al. (2026) · Discover Nano · doi:10.1186/s11671-026-04681-9 · Cited by 3
Metadata
- Authors
- Xu, Y., & Li, Z.
- Venue
- Computational and Structural Biotechnology Journal
- Published
- 2020
- Volume
- 18
- Pages
- 2401-2415
- ISSN
- 2001-0370 (ISSN-L)
- OpenAlex
- W3083369815
- Cited by
- 404
- References
- 57
- FWCI
- 6.02
- Type
- journal article
- Language
- en
- Open access
- gold
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