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Targeted Gene Manipulation in Plants Using the CRISPR/Cas Technology

Zhang, Dandan, Li, Zhenxiang, Li, Jian-Feng·2016·Journal of Genetics and Genomics

Abstract

The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse organisms including plants, and has quickly evolved into a suite of versatile tools for sequence-specific gene manipulations beyond genome editing. Here, we review the most recent applications of the CRISPR/Cas toolkit in plants and also discuss key factors for improving CRISPR/Cas performance and strategies for reducing the off-target effects. Novel technical breakthroughs in mammalian research regarding the CRISPR/Cas toolkit will also be incorporated into this review in hope to stimulate prospective users from the plant research community to fully explore the potential of these technologies.

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Metadata

Authors
Zhang, D., Li, Z., & Li, J.-F.
Venue
Journal of Genetics and Genomics
Publisher
Elsevier BV
Published
2016-05
Volume
43
Issue
5
Pages
251-262
ISSN
1673-8527
Cited by
67
References
10
Language
en
Funders
  • National Natural Science Foundation of China (31522006, 31570276)
  • Sun Yat-sen University
  • China's Thousand Young Talents Program

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