Cited by 58Cellular transport and secretionNeuroscience and Neuropharmacology ResearchExtracellular vesicles in disease

Molecular and functional architecture of striatal dopamine release sites

Open access
Banerjee, Aditi, Imig, Cordelia, Balakrishnan, Karthik, Kershberg, Lauren, Lipstein, Noa, Uronen, Riikka-Liisa, Wang, Jiexin, Cai, Xintong +7 more·2022·Neuron

Published version of a preprint

Abstract

Despite the importance of dopamine for striatal circuit function, mechanistic understanding of dopamine transmission remains incomplete. We recently showed that dopamine secretion relies on the presynaptic scaffolding protein RIM, indicating that it occurs at active zone-like sites similar to classical synaptic vesicle exocytosis. Here, we establish using a systematic gene knockout approach that Munc13 and Liprin-α, active zone proteins for vesicle priming and release site organization, are important for dopamine secretion. Furthermore, RIM zinc finger and C 2 B domains, which bind to Munc13 and Liprin-α, respectively, are needed to restore dopamine release after RIM ablation. In contrast, and different from typical synapses, the active zone scaffolds RIM-BP and ELKS, and RIM domains that bind to them, are expendable. Hence, dopamine release necessitates priming and release site scaffolding by RIM, Munc13, and Liprin-α, but other active zone proteins are dispensable. Our work establishes that efficient release site architecture mediates fast dopamine exocytosis.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.neuron.2021.10.028

Cites

117 of 117 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The specificity of anti-Munc13-1 and anti-bMunc13-2 antibodies was confirmed using knock-out animals.

    “The specificity of anti-Munc13-1 and anti-bMunc13-2 antibodies was confirmed using replicas of acute hippocampal slices prepared from Munc13-1cKO-Munc13-2/3(−/−) animals [110,111].”

    From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [111]

    The STAR Methods (page unavailable) describe Munc13 knockout mice and anti-Munc13-1 antibodies used for Western blots, but do not report validating anti-Munc13-1 and anti-bMunc13-2 antibodies on replicas of acute hippocampal slices. Searches for antibody specificity, anti-bMunc13-2, and hippocampal-slice replicas found no passage addressing that validation. (LLM, conf=0.75)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

1 retracted

Flagged first, then by citations

1 of the 118 references couldn’t be matched to a known paper.

Research works printed but not linked: 1

  1. Descarries L, Watkins KC, Garcia S, Bosler O, and Doucet G (1996). Dual character, asynaptic and synaptic, of the dopamine innervation in adult rat neostriatum: A quantitative autoradiographic and immunocytochemical analysis. J. Comp. Neurol 375, 167– 186. 8915824

Cited by

Sorted by citations

Authors
Banerjee, A., Imig, C., Balakrishnan, K., Kershberg, L., Lipstein, N., Uronen, R.-L., Wang, J., Cai, X., Benseler, F., Rhee, J. S., Cooper, B. H., Liu, C., Wojcik, S. M., Brose, N., & Kaeser, P. S.
Affiliations
  • Harvard University (US)
  • Max Planck Institute of Experimental Medicine (DE)
Corresponding author
Kaeser, Pascal S.
Venue
Neuron
Publisher
Elsevier BV
Published
2022-01
Volume
110
Issue
2
Pages
248-265.e9
ISSN
0896-6273 (ISSN-L)
OpenAlex
W3214185212
Cited by
58
References
118
Citations by year
2026: 15 · 2025: 10 · 2024: 10 · 2023: 10 · 2022: 9 · 2021: 4
FWCI
4.31
Type
journal article
Language
en
Open access
Locations
  • Neuron (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion)
Funders
  • DFG (EXC 2067/1-390729940)
  • China Scholarship Council
  • ERC
  • NIH (F31NS105159, R01MH113349, R01NS103484)
  • European Commission
  • Massachusetts Institute of Technology
  • Xi’an Jiaotong University
  • Harvard University
  • European Research Council (EXC 2067/1-390729940)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
dopamine release, striatum, RIM, Munc13, Liprin-α, active zone, synaptic vesicle exocytosis, vesicle priming, elk, gene knockout, dopamine signaling
MeSH terms
Corpus Striatum, Dopamine, Exocytosis, Synaptic Transmission, Synapses

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review