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Cited by 30Neuroendocrine regulation and behaviorSexual Differentiation and DisordersHypothalamic control of reproductive hormones

Absence of progestin receptors alters distribution of vasopressin fibers but not sexual differentiation of vasopressin system in mice

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Rood, B.D., Murray, E.K., Laroche, J., Yang, M.K., Blaustein, J.D., De Vries, G.J.·2008·Neuroscience
DOI 10.1016/j.neuroscience.2008.03.087PMC PMC4017668
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Abstract

Perinatal estrogens increase the number of vasopressin-expressing cells and the density of vasopressin-immunoreactive fibers observed in adult male rodents. The mechanism of action of estrogens on sexual differentiation of the extra-hypothalamic vasopressin system is unknown. We hypothesized that the sexually dimorphic expression of progestin receptors (PRs) during development would masculinize vasopressin expression in mice. We compared the number of vasopressin-expressing cells in the bed nucleus of the stria terminalis (BNST) and medial amygdala and the density of vasopressin-immunoreactive fibers in several brain regions of male and female wild type and PRKO mice using in situ hybridization and immunohistochemistry. As expected, sex differences in vasopressin cell number were observed in the BNST and medial amygdaloid nucleus. Vasopressin-immunoreactive fiber density was sexually dimorphic in the lateral septum, lateral habenular nucleus, medial amygdaloid nucleus, and mediodorsal thalamus. Sex differences were also observed in the principal nucleus of the BNST and medial preoptic area but not in the dorsomedial hypothalamus, which are thought to receive vasopressin innervation from the suprachiasmatic nucleus. Deletion of PRs did not alter the sex difference in vasopressin mRNA expression and vasopressin fiber immunoreactivity in any area examined. However, deletion of PRs increased the density of vasopressin fiber immunoreactivity in the lateral habenular nucleus. Our data suggest that PRs modulate vasopressin levels, but not sexual differentiation of vasopressin innervation in mice.

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Cited by

Sorted by citations

Authors
Rood, B. D., Murray, E. K., Laroche, J., Yang, M. K., Blaustein, J. D., & De Vries, G. J.
Affiliations
  • University of Massachusetts Amherst (US)
  • Amherst College (US)
ORCID
Venue
Neuroscience
Publisher
Elsevier BV
Published
2008-06
Volume
154
Issue
3
Pages
911-921
ISSN
0306-4522 (ISSN-L)
DOI
10.1016/j.neuroscience.2008.03.087
PMC
PMC4017668
OpenAlex
W2016403033
Cited by
30
References
11
Citations by year
2023: 3 · 2022: 3 · 2021: 4 · 2018: 2 · 2016: 1 · 2014: 1 · 2013: 5 · 2012: 3
FWCI
3.57
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute of Mental Health (T32 MH 47538, K02-MH01497, R01MH47538, R21MH067630, MH 067630, K02MH001497, R01MH047538)
  • National Institute of Neurological Disorders and Stroke (R01NS019327, NS-19327)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (U54HD028934, U54-HD-28934)
Topics
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Keywords
progestin receptor, vasopressin, sexual differentiation, bed nucleus of the stria terminalis, medial amygdala, sex differences, lateral septum, medial preoptic area, mediodorsal thalamus
MeSH terms
Animals, Cell Count, Drug Implants, Female, Immunohistochemistry, Male, Nerve Fibers, Receptors, Progesterone, RNA, Messenger, Sex Differentiation, Steroids, Testosterone, Vasopressins, Silver Staining, In Situ Hybridization, Mice, Knockout, Mice

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